Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 290,303 Δ1 bp 3.2% intergenic (‑36/+14) RCAP_rcc00253 ← / ← RCAP_rcc00254 major facilitator superfamily protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034290,3030G→. 3.2% 10.4 ‑1.6 ‑0.1187intergenic (‑36/+14)RCAP_rcc00253/RCAP_rcc00254major facilitator superfamily protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/1):  ref base (90/90):  total (95/92)
Fisher's exact test strand distribution p-value = 2.12e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.99e-01

Only 100 of 238 total aligned reads displayed.

AAGCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGATAGCC  >  NC_014034/290203‑290403
                                                                                                    |                                                                                                    
aaGCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCg                                                                                                      >  1:1093896/1‑101 (MQ=255)
 aGCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCgg                                                                                                     >  2:2834700/1‑101 (MQ=255)
 aGCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCgg                                                                                                     <  1:2825922/101‑1 (MQ=255)
  gCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATGAGGCGGGGCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCggg                                                                                                    >  2:3987333/1‑101 (MQ=255)
  gCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCggg                                                                                                    >  1:1922737/1‑101 (MQ=255)
    caggCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCggggg                                                                                                  >  1:2421026/4‑101 (MQ=255)
    cAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCggggg                                                                                                  >  2:1574243/1‑101 (MQ=255)
    cAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCggggg                                                                                                  >  1:329404/1‑101 (MQ=255)
     aaGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCgggggg                                                                                                 >  1:1051125/1‑101 (MQ=255)
     aaGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCgggggg                                                                                                 >  1:1819991/1‑101 (MQ=255)
       gCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCgggggggg                                                                                               >  1:1646833/1‑101 (MQ=255)
        cATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCggggggggc                                                                                              <  1:4031151/101‑2 (MQ=255)
         aTCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGc                                                                                             >  2:2155131/1‑101 (MQ=255)
           cAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTg                                                                                           >  1:2113717/1‑101 (MQ=255)
           cAAGATAATGGAGCCGCCGAGAAGGCGGGGGGGAAACAGGCGGGTCTGGGTCATTTGGTCTCCCCAGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTg                                                                                           <  2:2963324/101‑1 (MQ=255)
            aaGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGc                                                                                          >  2:2628847/1‑101 (MQ=255)
                  atgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAAt                                                                                    >  1:3533797/1‑101 (MQ=255)
                  agggCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCCCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAAt                                                                                    <  1:1989383/99‑1 (MQ=255)
                    ggCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCt                                                                                  >  1:101487/1‑101 (MQ=255)
                    ggCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGC.GGGGGGGGCTGCGTCAATCTg                                                                                 <  2:3750380/101‑1 (MQ=255)
                    ggCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCCCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCt                                                                                  <  1:3158484/101‑1 (MQ=255)
                      cgccgcCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGt                                                                                <  2:2195927/101‑1 (MQ=255)
                        ccgccCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCg                                                                              <  1:2614183/101‑1 (MQ=255)
                         cgccCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGa                                                                             >  1:607673/1‑101 (MQ=255)
                          gccCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGAt                                                                            <  2:1763956/101‑1 (MQ=255)
                           cccACAAGGGCGGGGGTGGTCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGGGGGGGGGGGCTGCGTCAATCTGTCGAtt                                                                           <  1:2629039/101‑1 (MQ=255)
                             cacaAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATtgt                                                                         >  1:2355885/1‑101 (MQ=255)
                             cacaAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATtgt                                                                         <  1:2774933/101‑1 (MQ=255)
                              acaAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATtgtg                                                                        >  2:814678/1‑101 (MQ=255)
                              acaAGGACGGGGGTGATCAGGCGGGTCTGGGGCATTTGGTCTCCTTGGCAAGTGGGCAGGTTGACCATTGGGGGGGGGGTGGCTTAAATCTGTCGTTggtg                                                                        >  2:4211652/1‑101 (MQ=255)
                                aaGGACGGGGGTGATCAGGCGGGGCTGGGTCATTTGGGCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCGGCGTCAATCTCTCTAATCTGcc                                                                      >  2:639685/1‑101 (MQ=255)
                                 aGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGccc                                                                     >  2:3569967/1‑101 (MQ=255)
                                  tctcGGGGGTGATCAGGCGGGTCTGGGTCATTTGGCCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGcccc                                                                    <  2:1508441/98‑1 (MQ=255)
                                   gACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCg                                                                   <  2:3363046/101‑1 (MQ=255)
                                   gACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCg                                                                   >  1:2703751/1‑101 (MQ=255)
                                    aCGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGa                                                                  >  2:2585784/1‑101 (MQ=255)
                                     cGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGccccaga                                                                 >  1:1508545/1‑98 (MQ=255)
                                        gggTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACaa                                                              <  2:627217/101‑1 (MQ=255)
                                          gTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATc                                                            >  1:3903088/1‑101 (MQ=255)
                                            gATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATcgc                                                          >  1:119843/1‑101 (MQ=255)
                                               cAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgcc                                                       <  2:1051031/101‑1 (MQ=255)
                                               cAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGC.GGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgccg                                                      >  2:3123688/1‑101 (MQ=255)
                                                aGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgccg                                                      <  2:4037826/101‑1 (MQ=255)
                                                aGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGC.GGGGGGGGCTGCGGCAATCTTTCGGTTGTGGCCCCAACAATCGcgccgc                                                     >  2:3853234/1‑101 (MQ=255)
                                                 ggCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGGTGCGTCAATCTGTCGATTTTGCCCCGAAACATCGcgccgc                                                     >  1:3010266/1‑101 (MQ=255)
                                                 ggCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgccgc                                                     <  1:2940114/101‑1 (MQ=255)
                                                 ggCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGCGCCCC.AA.AATCGcgcccccc                                                   >  2:1413927/1‑100 (MQ=255)
                                                  gCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgccgcc                                                    >  1:3204215/1‑101 (MQ=255)
                                                   cGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgccgccg                                                   <  2:3199491/101‑1 (MQ=255)
                                                    gggTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgccgccgc                                                  >  2:441733/1‑101 (MQ=255)
                                                     ggTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCgccgccgcc                                                 >  2:1593168/1‑101 (MQ=255)
                                                       tCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTg                                               >  1:880625/1‑101 (MQ=255)
                                                        cTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGc                                              >  2:1356215/1‑101 (MQ=255)
                                                        cTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGc                                              >  1:1320690/1‑101 (MQ=255)
                                                         tGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCgccgccgccaccc                                             <  1:1585072/101‑5 (MQ=255)
                                                         tGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGcc                                             <  1:2834919/101‑1 (MQ=255)
                                                         tGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGcc                                             >  1:3789046/1‑101 (MQ=255)
                                                         ggggTCTTTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCTGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGcc                                             <  2:3510781/100‑1 (MQ=255)
                                                          gggTCATTGGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGccc                                            <  2:1497311/101‑1 (MQ=255)
                                                            gTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCgg                                          <  1:2155276/101‑1 (MQ=255)
                                                             tCATTTGGACTCCCGCGCAAGGCGGCCAGTGCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGc                                         <  2:1213377/101‑1 (MQ=255)
                                                              cATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCg                                        >  1:2731676/1‑101 (MQ=255)
                                                              cATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCg                                        >  2:252921/1‑101 (MQ=255)
                                                               aTTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGa                                       >  1:4053665/1‑101 (MQ=255)
                                                                 ttGGTCTCCCTCGCAAGGCGGCCGGTTCGCCATGGGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGAcg                                     <  1:256028/101‑1 (MQ=255)
                                                                 ttGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGAcg                                     <  2:2882251/101‑1 (MQ=255)
                                                                 ttGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGAcg                                     <  2:1093802/101‑1 (MQ=255)
                                                                 ttGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGGCCATTGTGCCCCGAACAATCGCGCCACCGCCTGCCCCGCGAcg                                     >  2:3807490/1‑101 (MQ=255)
                                                                  tGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGAcgc                                    >  2:2303602/1‑101 (MQ=255)
                                                                  tGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGAcgc                                    <  1:4224576/101‑1 (MQ=255)
                                                                   ggTCTCCCTCGCAAGGCGGCCAGTTCTCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGAcgcg                                   <  1:1358618/101‑1 (MQ=255)
                                                                   ggTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGTTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGAcgcg                                   <  2:1687355/101‑1 (MQ=255)
                                                                    gTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCgg                                  >  1:2269109/1‑101 (MQ=255)
                                                                     tctcCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCCCGCCGCCGCCTGCCCGGCGACGCGGc                                 >  2:589006/1‑101 (MQ=255)
                                                                        cccTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCg                              >  1:1509010/1‑101 (MQ=255)
                                                                          cTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTc                            <  1:814759/101‑1 (MQ=255)
                                                                            cGCAAGGCGGCGAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGc                          <  2:265850/101‑1 (MQ=255)
                                                                             gCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGcc                         >  2:531024/1‑101 (MQ=255)
                                                                               aaGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAg                       >  2:969405/1‑101 (MQ=255)
                                                                                  gcggcCAGTTCGCCAGGGGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACa                    <  1:890816/101‑1 (MQ=255)
                                                                                  gcgccCGGTTCGCCATG.GGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAg                   <  1:3853493/101‑1 (MQ=255)
                                                                                    ggcCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCGGCCGCCTGCCCGGCGACGCGGGCCGCCGCCAGACAGc                  >  2:3285762/1‑101 (MQ=255)
                                                                                    ggcCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGc                  <  2:313716/101‑1 (MQ=255)
                                                                                      ccAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCg                >  1:3674218/1‑101 (MQ=255)
                                                                                      ccAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCg                >  1:85852/1‑101 (MQ=255)
                                                                                      ccAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGCGCCCCGAACAATCGCGCCGTCGCTTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCg                >  1:3618521/1‑101 (MQ=255)
                                                                                       cAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCgg               >  2:2545860/1‑101 (MQ=255)
                                                                                         gTTCGCCATGCGGGGGGGGGCCGCGTCAATCTGTCGATTTGGCCCCGAACAATCGCGCCGCCCCCTGCCCGGGGACGCGGCCCCTCGCCCGCCACCCgggc             >  2:1845309/1‑99 (MQ=255)
                                                                                          ttCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTca            <  2:2948287/101‑1 (MQ=255)
                                                                                          ttCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGGca            >  1:3727891/1‑101 (MQ=255)
                                                                                           tCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTcag           <  2:102997/101‑1 (MQ=255)
                                                                                            cGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTcagc          >  2:1522439/1‑101 (MQ=255)
                                                                                            cGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTcagc          >  2:2095485/1‑101 (MQ=255)
                                                                                              ccATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTcagcag        <  1:638588/101‑1 (MQ=255)
                                                                                              ccATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTcagcag        >  2:1657204/1‑101 (MQ=255)
                                                                                              ccATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTcagcag        <  2:2703545/101‑1 (MQ=255)
                                                                                               cATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGa       <  1:969496/101‑1 (MQ=255)
                                                                                                 tgggggggggggCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGATa     <  1:532490/98‑1 (MQ=255)
                                                                                                   cGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGATAgc   >  2:668032/1‑101 (MQ=255)
                                                                                                    gggggggggCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGATAgcc  <  1:151771/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
AAGCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGATAGCC  >  NC_014034/290203‑290403

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG