Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,254,687 Δ1 bp 100% intergenic (+12/‑171) glpX → / → recJ fructose‑1,6‑bisphosphatase/single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,254,6870G→.100.0% 52.9 107intergenic (+12/‑171)glpX/recJfructose‑1,6‑bisphosphatase/single‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (1/88):  ref base (17/0):  total (19/88)
Fisher's exact test strand distribution p-value = 9.22e-19
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.70e-10

Only 100 of 144 total aligned reads displayed.

ACCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.CCGGGGGGTATGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTG  >  NC_014034/3254587‑3254780
                                                                                                     |                                                                                               
 cccGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGGGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgg                                                                                                >  1:1919069/1‑101 (MQ=255)
   cGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACcggg                                                                                              >  2:653176/1‑101 (MQ=255)
    gAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACggggg                                                                                             >  2:154752/1‑101 (MQ=255)
     aaaTTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACggggga                                                                                            <  1:3779224/101‑2 (MQ=255)
     aaaTTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACggggga                                                                                            >  2:2549896/1‑100 (MQ=255)
       aTTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggact                                                                                          <  2:662073/101‑4 (MQ=255)
       aTTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggact                                                                                          <  1:1752695/101‑4 (MQ=255)
        ttCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCTACCcgggggcg                                                                                          >  2:1546865/1‑99 (MQ=255)
        ttCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggactg                                                                                         <  1:3294858/101‑5 (MQ=255)
         tCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggggggc                                                                                        >  1:1762419/1‑97 (MQ=255)
         tCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggactgc                                                                                        <  2:2883316/101‑6 (MQ=255)
         tCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACcgggggcgcc                                                                                        >  1:3378401/1‑97 (MQ=255)
          cATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCTACCgggggggggc                                                                                        >  1:2695307/1‑97 (MQ=255)
          cATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCTACCgggggggcgc                                                                                        >  2:2291687/1‑97 (MQ=255)
          cATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggactgcc                                                                                       <  2:1006146/101‑7 (MQ=255)
           aTCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggggcggcc                                                                                      >  2:2277552/1‑95 (MQ=255)
             cGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGCTCCGCTGAGGCAACGNC.ACgggggtctncnnnn                                                                                    >  2:1414957/1‑92 (MQ=255)
               ggaCCGAAACGATCCTGATGCGATCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTccc                                                                                 <  1:818320/100‑1 (MQ=255)
               agaCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCTACCggggggggccggccc                                                                                   >  1:2374824/1‑92 (MQ=255)
                gaCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTcccc                                                                                <  1:443784/101‑1 (MQ=255)
                 aCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTccccc                                                                               <  2:516546/101‑1 (MQ=255)
                 aCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTccccc                                                                               <  1:129560/101‑1 (MQ=255)
                 aCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTccccc                                                                               <  2:3311283/101‑1 (MQ=255)
                    gAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GTGGGACTGCCGTCCCCCGCa                                                                            <  2:332946/101‑1 (MQ=255)
                     aaaCGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCAc                                                                           <  1:578331/101‑1 (MQ=255)
                      aaCGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCAcc                                                                          <  2:884382/101‑1 (MQ=255)
                       aCGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCACCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCAccc                                                                         <  1:489362/101‑1 (MQ=255)
                       aCGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCAccc                                                                         <  1:2550087/101‑1 (MQ=255)
                        cGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCAcccc                                                                        <  2:1445440/101‑1 (MQ=255)
                           tCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTg                                                                     <  1:3966850/101‑1 (MQ=255)
                            ccTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTgc                                                                    <  1:3129750/101‑1 (MQ=255)
                              tGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTgcgc                                                                  <  1:2457509/101‑1 (MQ=255)
                                aTGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGt                                                                <  2:645650/101‑1 (MQ=255)
                                aTGCGCTCGAAAACCGGCTCGGGGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.GTGCCGTCCCCCCCCCCCCCCCcgcgc                                                                >  1:588972/1‑100 (MQ=255)
                                    gcTCGAAAACCGTCTCGGTGCGCCGGGTGATCTATCGCACCCCGATCCGCTGAGGCAACGCT.CC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.ttt                                                           <  2:4032380/101‑1 (MQ=255)
                                    gcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.ttt                                                           <  1:237258/101‑1 (MQ=255)
                                    gcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.ttt                                                           <  2:2767783/101‑1 (MQ=255)
                                    gcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.ttt                                                           <  1:798979/101‑1 (MQ=255)
                                         aaaaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.Ca                                                     <  2:1739656/101‑1 (MQ=255)
                                         aaaaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.Ca                                                     <  2:3638232/101‑1 (MQ=255)
                                          aaaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.Caa                                                    <  1:2784905/101‑1 (MQ=255)
                                           aaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCCGCCCCCCCCCCCCCCGCGCGGA.TTTTTc.ccaa                                                   >  2:3463162/1‑98 (MQ=255)
                                           aaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAg                                                   <  1:758377/101‑1 (MQ=255)
                                               gtcTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGaaa                                               <  2:2217131/99‑1 (MQ=38)
                                               ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGGCGCCGGCCCCCCCCCCCCCCCCCCGTC.TTTTGC.Cacgtaa                                                >  2:3322313/1‑96 (MQ=255)
                                               ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGaaa                                               <  2:3443484/101‑1 (MQ=38)
                                               ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGaaa                                               <  1:3474576/101‑1 (MQ=38)
                                               ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGaaa                                               <  2:912067/101‑1 (MQ=38)
                                               ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGaaa                                               <  2:2081779/101‑1 (MQ=38)
                                                gCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCCGCCCCCCCCCCCCCCCCGCGTG.TTTTTC.CCAGGaaaa                                              >  2:368613/1‑101 (MQ=255)
                                                gCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGaaaa                                              <  1:3421783/101‑1 (MQ=38)
                                                  tCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACg                                            <  1:3682707/101‑1 (MQ=38)
                                                  tCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACg                                            <  1:1513635/101‑1 (MQ=38)
                                                   cGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACgg                                           <  2:249460/101‑1 (MQ=38)
                                                    ggTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.GTGCCGGCCCCCCCCCCCCCCCCCCGCC.TTTTTC.CCAGGAAAAAggg                                          >  1:1859035/1‑101 (MQ=255)
                                                    ggTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACCGGGGGGGGGCGGCCCCCCCCCCCCCCCCCCCCCC.TTTTTC.CAAGGaaaaaag                                           >  2:2207472/1‑98 (MQ=255)
                                                     gTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACgggg                                         <  1:2207301/101‑1 (MQ=38)
                                                      tGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGA.GCCGTCCCCCCCCCCCCCCCCGCGCA.TTTTTT.CAAGGAAAAAAGGGc                                        >  1:1131513/1‑101 (MQ=255)
                                                      tGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGc                                        <  1:54828/101‑1 (MQ=38)
                                                       gcgcCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGcc                                       <  2:730245/101‑1 (MQ=38)
                                                       gcgcCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGcc                                       <  1:3153603/101‑1 (MQ=38)
                                                         gcCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCACCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGt                                     <  1:1132523/101‑1 (MQ=38)
                                                          ccGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGtt                                    <  1:653253/101‑1 (MQ=38)
                                                           cGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGttt                                   >  1:3908832/1‑101 (MQ=38)
                                                           cGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGACCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGttt                                   <  2:197639/101‑1 (MQ=38)
                                                            gggTGATCTATCGCACCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCCCCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTc                                  <  1:3469453/101‑1 (MQ=38)
                                                            gggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTc                                  <  2:712743/101‑1 (MQ=38)
                                                            gggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTc                                  <  2:4012417/101‑1 (MQ=38)
                                                             ggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCCCCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGTCCGTTTCa                                 <  2:605702/101‑1 (MQ=38)
                                                             ggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTCa                                 <  2:602518/101‑1 (MQ=38)
                                                              gTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.GTGCCGTCCCCCCCACCCCCCCCGCGCATTTTTCC.CAAGAAAAAAGGGGcgggtctc                                 >  2:3276193/1‑94 (MQ=255)
                                                              gTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.GTCCCGGCCCCCGCCCCCCCCCCGCGTT.TTTTTG.CAAGGAAAAAGGGGGCGGTTGAc                                >  2:3522992/1‑101 (MQ=255)
                                                              gTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGcctctgcca                                <  2:3306716/101‑8 (MQ=37)
                                                               tGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGGCGTCCCCCGCCCCCCCCCCGCGCA.TTTTGC.CAAGGGAAACGGGGGCGTTTCCcc                               >  2:2598823/1‑101 (MQ=255)
                                                               tGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGGCCCCCCCACCCCCCGCGCGGA.TTTTGC.CAACGAAAACCGGGGCGGTTCAcc                               >  2:1056263/1‑101 (MQ=255)
                                                                 aTCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTCACCtt                             <  2:1206395/101‑1 (MQ=38)
                                                                  tCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTCACCTTg                            <  2:2482124/101‑1 (MQ=38)
                                                                    tATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTCACCTTGGc                          <  1:346560/101‑1 (MQ=38)
                                                                       cgaaaCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCTCCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTCACCTTGGCCGa                       <  2:1157432/98‑1 (MQ=38)
                                                                       cGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTCACCTTGGCCGa                       <  2:949579/101‑1 (MQ=38)
                                                                        gagaCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGCACCCCGCTCCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTCACCTTGGCCGaa                      <  1:2163952/98‑1 (MQ=37)
                                                                        gCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGGGGCGGCCCCCCCCCCCCCCCCCCCGCG.TTTTTG.CAGGGAAAACGGGGCGGTTCTCCCCTGGCCgc                       >  1:422436/1‑100 (MQ=255)
                                                                        gCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTCACCTTGGCCGaa                      <  1:1913470/101‑1 (MQ=38)
                                                                          aaCCCGATCCGCTGAGGCAACGCT.CC.GGGGGCCTGCCGGCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAat                    <  1:4049177/101‑1 (MQ=38)
                                                                           aCCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CCGCCGCCCCCCGCCCCCCCCGCGCGGA.TTTTGT.CAAAGAAAACGGGGCCGCTTCACCCTGGCCGacaca                   >  2:2053847/1‑97 (MQ=255)
                                                                            cccGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTCACCCTGGCCGAAatat                  <  2:2695101/101‑1 (MQ=38)
                                                                              cGATCCGCTGAGGCAACGCT.ACGGGGGG.GGGCCGGCCCCCCCCCCCCCCCCGCGGA.TTTTTCTCAGGAAAAAAGGGGCGGTTTC.CCTTTGCCGCAAAATcc                >  1:2624035/1‑101 (MQ=255)
                                                                              cGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATcc                <  2:2858568/101‑1 (MQ=38)
                                                                               gATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATccc               <  2:2129508/101‑1 (MQ=38)
                                                                               gATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATccc               <  2:864603/101‑1 (MQ=38)
                                                                                aTCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCg              <  2:2567984/101‑1 (MQ=38)
                                                                                 tCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCgg             <  2:1859590/101‑1 (MQ=38)
                                                                                     cTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCgggggg         <  1:1227345/101‑1 (MQ=38)
                                                                                       gAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGa       <  2:1737455/101‑1 (MQ=38)
                                                                                        aGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGaa      <  2:1538256/101‑1 (MQ=38)
                                                                                         ggCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAAt     <  2:2832792/101‑1 (MQ=38)
                                                                                          gCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAAtt    <  1:64055/101‑1 (MQ=38)
                                                                                          gCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAAtt    <  1:3314392/101‑1 (MQ=38)
                                                                                          gCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAAtt    <  2:3909891/101‑1 (MQ=38)
                                                                                            aaCGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTg  <  2:2581988/101‑1 (MQ=38)
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ACCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.CCGGGGGGTATGCCGTCCCCCGCACCCCCTGCGCGTA.TTTTGC.CAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTG  >  NC_014034/3254587‑3254780

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG