BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	408,487	Δ1 bp	100%	intergenic (+105/+43)	glnA1 → / ← dapA	glutamine synthetase/dihydrodipicolinate synthase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	408,487	0	A→.	100.0%	478.1	145	intergenic (+105/+43)	glnA1/dapA	glutamine synthetase/dihydrodipicolinate synthase
Reads supporting (aligned to +/- strand): new base (92/50): ref base (1/1): total (94/51)

Only 100 of 165 total aligned reads displayed.


CTGGGGGCTCTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCAA.AGACGGGG..G.....GC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGC  >  NC_014034/408387‑408568


cagggcgCTCTGCCCCCAGACCCCTGGGGAATTTGCGGCAGGATGAAGCCGATGTCCTTCATCTTGCAGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCa                                                                                                <  2:2714523/99‑1 (MQ=25)


 tGGGGGCTCTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCaa                                                                                               <  2:394060/101‑1 (MQ=255)


  gggggCTCTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCaag                                                                                              <  2:3247817/101‑2 (MQ=255)


  gggggCTCTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCaag                                                                                              >  1:1816371/1‑100 (MQ=255)


   ggggCCCCGCCCCCAGGCCCCCGGGATTTTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCaaga                                                                                             <  1:1655760/101‑3 (MQ=25)


    ccgatctGCCCCCAGCCCCCCGGGATATTGGCGGCAAGATAAGGCCGATGTCCTTCATCTTGCCGCAAATTTCCCGGGGGGAGGCTTGCTGAAAGCaagac                                                                                            <  1:3028721/97‑4 (MQ=25)


     ggCTCTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCaagacg                                                                                           <  2:729607/101‑5 (MQ=255)


      gCTCTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATTCCGGGGGGAGGCTTGCTGAAAGCaagaccg                                                                                          >  2:3775853/1‑96 (MQ=255)


        tctGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCaagactggg                                                                                        >  1:3790936/1‑94 (MQ=255)


        tctGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATACCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACgggg                                                                                      >  1:3657348/1‑101 (MQ=255)


          tGCCCCCAGACCCCCGGGGTATTTGCGGGAAGAATAAACCGACGTTCCTTATTTTGCCGCCAAAAACCCCGGGGGGGGGTGTCCGAAAAAAA.AAACgggg..g                                                                                   >  2:853569/1‑101 (MQ=255)


          cccccccGGCCCCCGGGGATTTTTGGGGAAAAATAAGGCCGATTTCTTTCATTTTCCCGCAAATTTCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACgggg..g.....g                                                                             <  1:1592805/99‑1 (MQ=255)


            cccccAGCCCCCCGGGATATTTGGGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGACTTGCTGAAAGC.A.AGACGGGG..G.....Gca                                                                           <  1:4025070/101‑2 (MQ=25)


              cccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..G.....Gcaga                                                                         <  1:2468328/101‑4 (MQ=255)


              cccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..G.....Gcaga                                                                         <  1:1377659/101‑4 (MQ=255)


              cccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..G.....Gcaga                                                                         <  2:3358852/101‑4 (MQ=255)


               ccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..G.....Gcagag                                                                        >  2:2967539/1‑97 (MQ=255)


                 agcccccgGGGATTTTTGCGGAAAGAGAAAGCCGATGTCCTTAACTTTGCCGAAAATTTCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..G.....Gcagagcc                                                                      <  1:3196961/98‑7 (MQ=255)


                 aGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..G.....Gcagagcc                                                                      >  2:4153993/1‑95 (MQ=255)


                 aGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAG.AA.AGACGGGG..G.....Gcagagag                                                                      >  2:3261970/1‑95 (MQ=255)


                    ccccgGGTATTTTGGCGGAAAAATGAACCCGTTTCCTTTCATCTTCCCAAAAATCCCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..G.....Gcagagccccc                                                                   <  1:879360/101‑10 (MQ=255)


                     ccccGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..G.....Gcagagcccccc                                                                  <  2:2782432/101‑11 (MQ=25)


                     ccccGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..G.....Gcagagcccccc                                                                  >  2:1859944/1‑91 (MQ=25)


                                  gCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCa                                                     >  2:2067999/1‑101 (MQ=21)


                                  gCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCa                                                     >  1:3474698/1‑101 (MQ=21)


                                   cGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCaa                                                    >  1:586166/1‑101 (MQ=21)


                                   cGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCaa                                                    <  2:962094/101‑1 (MQ=21)


                                   cGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCaa                                                    >  1:1194680/1‑101 (MQ=21)


                                    ggCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCaaa                                                   >  1:2669173/1‑101 (MQ=21)


                                    ggCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCaaa                                                   >  1:2603345/1‑101 (MQ=21)


                                     gaaaGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCaaaa                                                  <  2:3597311/99‑1 (MQ=21)


                                     gCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCaaaa                                                  >  2:1244510/1‑101 (MQ=21)


                                      cAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAg                                                 >  2:3658910/1‑101 (MQ=21)


                                      cAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAg                                                 >  2:2452975/1‑101 (MQ=21)


                                       aaGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAgg                                                >  1:342787/1‑101 (MQ=21)


                                        aGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGga                                               >  1:574909/1‑101 (MQ=21)


                                        aGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGCCGCCAAAAGga                                               >  2:460123/1‑101 (MQ=18)


                                         gaggAAGCCGATGTCCTTGATCTTGCCGCAAATAGCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGgac                                              <  1:631105/98‑1 (MQ=255)


                                         gATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGGAGAGC.....CCCCCGCACAGGGCGCCAAAAGgac                                              >  2:1508479/1‑101 (MQ=21)


                                         gATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGgac                                              >  1:2759788/1‑101 (MQ=21)


                                         gATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGgac                                              <  1:1949175/101‑1 (MQ=21)


                                           tGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGgaccg                                            >  1:2826789/1‑101 (MQ=21)


                                            gAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGgaccga                                           >  1:2783109/1‑101 (MQ=21)


                                            gAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGgaccga                                           <  2:1137507/101‑1 (MQ=21)


                                             aaGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGgaccgac                                          >  2:2858809/1‑101 (MQ=21)


                                              accagATGCCCTTAATCTTGCCGCAAATCTCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGgaccgacc                                         <  1:3854971/97‑1 (MQ=19)


                                              aGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGgaccgacc                                         >  1:1031664/1‑101 (MQ=21)


                                               gCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCt                                        >  2:2256487/1‑101 (MQ=21)


                                               gCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GACAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCt                                        >  2:2825734/1‑101 (MQ=21)


                                               gCCGATGTCCTTCATCTTGCCGCAAATACCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCt                                        <  2:1816251/101‑1 (MQ=21)


                                                ccGATGTCCTTCATTTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCAA.GAAGGGGG..GCAGA.GC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTc                                       <  1:4182524/101‑1 (MQ=21)


                                                 cGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCa                                      >  1:107444/1‑101 (MQ=21)


                                                 cGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCa                                      >  2:2480062/1‑101 (MQ=21)


                                                 cGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCa                                      <  2:3417897/101‑1 (MQ=21)


                                                 cGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCa                                      >  1:2055735/1‑101 (MQ=21)


                                                  gATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAg                                     <  2:2747460/101‑1 (MQ=21)


                                                  gATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAg                                     <  2:3830015/101‑1 (MQ=21)


                                                   aTGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGa                                    <  1:2457365/101‑1 (MQ=21)


                                                   aTGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGa                                    >  2:2332950/1‑101 (MQ=21)


                                                   aTGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGa                                    >  2:409701/1‑101 (MQ=21)


                                                    tGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGAt                                   <  1:976197/101‑1 (MQ=21)


                                                    tGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGAt                                   >  1:1249390/1‑101 (MQ=21)


                                                    ggcccTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGAt                                   <  2:2485896/98‑1 (MQ=18)


                                                     gcccTTCATCTGGACGCAAATACCACGGGGGGAGGCTTGCTGAAAGCAAGAGGGGGGGCAGA....GC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATc                                  <  2:1631936/99‑1 (MQ=255)


                                                      tCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCa                                 <  1:1169721/101‑1 (MQ=21)


                                                      tCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCa                                 <  1:432479/101‑1 (MQ=21)


                                                       ccTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCTACCTCAGATCAg                                >  2:3778110/1‑101 (MQ=18)


                                                       ccTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAg                                >  1:1998602/1‑101 (MQ=21)


                                                       ccTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAg                                >  1:622243/1‑101 (MQ=21)


                                                        cTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGc                               >  1:3803174/1‑101 (MQ=21)


                                                           cATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCg                            >  2:19976/1‑101 (MQ=21)


                                                           cATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCg                            >  2:1428358/1‑101 (MQ=21)


                                                           cATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCg                            >  2:1424036/1‑101 (MQ=21)


                                                           cATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCg                            >  2:3335189/1‑101 (MQ=21)


                                                            aTCTTGCGGCAAATATCTCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCgg                           <  2:2608844/101‑1 (MQ=255)


                                                            aTCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAAccccg                           >  1:782389/1‑99 (MQ=21)


                                                             tCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGc                          <  2:1365626/101‑1 (MQ=21)


                                                             tCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGc                          >  1:3785937/1‑101 (MQ=21)


                                                              cTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCg                         <  1:228935/101‑1 (MQ=25)


                                                                tGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..G.....GCAGAGGCCCCCCCACAGGTCGCCAAAAGGGCCGACCTCAGATCAGCCCGGCgtg                       >  1:14229/1‑101 (MQ=21)


                                                                 gCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCgtgt                      <  1:223354/101‑1 (MQ=25)


                                                                  ccGCAAATTTCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGtt                     <  1:2231206/101‑1 (MQ=255)


                                                                  ccGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGtt                     <  1:3276284/101‑1 (MQ=25)


                                                                       aaTATCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATc                >  2:1930649/1‑101 (MQ=255)


                                                                        atatCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATcg               >  2:1627531/1‑101 (MQ=255)


                                                                           tCCCGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCgcgg            >  2:3867694/1‑101 (MQ=255)


                                                                             ccGGGGGGAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCgcggcg          <  2:1799479/101‑1 (MQ=255)


                                                                               ggggggAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGAACTCAGATCAGCCCGGCGTGTTGCATCGCGGCGta        >  2:3023796/1‑100 (MQ=255)


                                                                                gggggAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGGGTCa       >  2:174247/1‑101 (MQ=255)


                                                                                gggggAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCa       >  2:193211/1‑101 (MQ=255)


                                                                                gggggAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCa       >  2:2983468/1‑101 (MQ=255)


                                                                                 ggggAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCaa      <  1:4154255/101‑1 (MQ=255)


                                                                                  gggAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAg     >  2:3722250/1‑101 (MQ=255)


                                                                                  gggAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAg     <  2:3087060/101‑1 (MQ=255)


                                                                                  gggAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAg     >  2:56671/1‑101 (MQ=255)


                                                                                   ggAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgc    >  1:380876/1‑101 (MQ=255)


                                                                                    gAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgcg   >  2:2020580/1‑101 (MQ=255)


                                                                                    gAGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgcg   >  1:3154481/1‑101 (MQ=255)


                                                                                     aGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgcgc  >  1:1369964/1‑101 (MQ=255)


                                                                                     aGGCTTGCTGAAAGC.A.AGACGGGG..GGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgcgc  >  1:893713/1‑101 (MQ=255)


CTGGGGGCTCTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCAA.AGACGGGG..G.....GC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGC  >  NC_014034/408387‑408568

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG