Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 549,629 Δ1 bp 100% coding (470/471 nt) greA → transcription elongation factor GreA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034549,6290A→.100.0% 649.6 149coding (470/471 nt)greAtranscription elongation factor GreA
Reads supporting (aligned to +/- strand):  new base (82/65):  ref base (1/0):  total (84/65)

Only 100 of 170 total aligned reads displayed.

CTGGCCCGCGCGCTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGTAAAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGCCCGTGCCGCA  >  NC_014034/549529‑549725
                                                                                                    |                                                                                                
 tGGCCCGCGCGCTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGTaa                                                                                                 <  2:4074438/101‑1 (MQ=255)
  ggCCCGCGCGCTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGTaag                                                                                                <  2:3755070/101‑2 (MQ=255)
  ggCCCGCGCGCTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGTaag                                                                                                >  1:649928/1‑100 (MQ=255)
   gCCCGCGCGCTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGTaagc                                                                                               >  2:3673228/1‑99 (MQ=255)
   gCCCGCGCGCTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGTaagc                                                                                               <  2:617296/101‑3 (MQ=255)
    cccGCGCGCTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGTaagcg                                                                                              <  1:2417612/101‑4 (MQ=255)
     ccGCGCGCTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGTaagcgg                                                                                             <  2:1482334/101‑5 (MQ=255)
     catccagcTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGTaagcgg                                                                                             >  2:2024087/7‑97 (MQ=255)
        cgcgcTGATCGGCAAGGACGAAGGCGACAGNGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGcc                                                                                         <  1:266569/101‑1 (MQ=255)
         gcgcTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGccc                                                                                        <  1:3196459/101‑1 (MQ=255)
         gcgcTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGccc                                                                                        <  1:4181076/101‑1 (MQ=255)
          cgcTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGcccc                                                                                       <  2:2438324/101‑1 (MQ=255)
          cgcTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGcccc                                                                                       >  2:87104/1‑101 (MQ=255)
          cgcTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGcccc                                                                                       >  2:3029713/1‑101 (MQ=255)
            cTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCg                                                                                     >  1:573141/1‑101 (MQ=255)
             tGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCgc                                                                                    >  2:1226654/1‑100 (MQ=255)
             tGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGa                                                                                    <  1:1572504/101‑1 (MQ=255)
              gATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGAc                                                                                   <  2:76722/101‑1 (MQ=255)
               aTCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGAcc                                                                                  >  2:1799034/1‑101 (MQ=255)
                tCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCa                                                                                 <  2:520163/101‑1 (MQ=255)
                tCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCa                                                                                 <  1:1622207/101‑1 (MQ=255)
                tCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCa                                                                                 >  2:164386/1‑101 (MQ=255)
                tCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCa                                                                                 <  2:2675814/101‑1 (MQ=255)
                 cGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATACTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCaa                                                                                <  2:1443048/101‑1 (MQ=255)
                  ggCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAg                                                                               >  2:4007417/1‑101 (MQ=255)
                   gCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAgg                                                                              <  2:976056/101‑1 (MQ=255)
                    cAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGa                                                                             >  1:1109304/1‑101 (MQ=255)
                    cAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGa                                                                             <  1:4114196/101‑1 (MQ=255)
                      aGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATg                                                                           <  2:3901429/101‑1 (MQ=255)
                      aGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATg                                                                           <  1:2444302/101‑1 (MQ=255)
                         aCGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACg                                                                        <  1:2714955/101‑1 (MQ=255)
                         aCGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACg                                                                        <  1:3441113/101‑1 (MQ=255)
                          cGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACga                                                                       <  2:2973522/101‑1 (MQ=255)
                           gAAGGCGACAGCGTCGAGGGCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACgag                                                                      >  2:429914/1‑101 (MQ=255)
                              ggCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATg                                                                   <  2:3751025/101‑1 (MQ=255)
                               gCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATgg                                                                  <  2:2696232/101‑1 (MQ=255)
                                cGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATggg                                                                 >  2:3931749/1‑101 (MQ=255)
                                 gACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGc                                                                >  1:1462237/1‑101 (MQ=255)
                                   cAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGa                                                              >  2:423802/1‑101 (MQ=255)
                                    aGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAg                                                             >  2:2218723/1‑101 (MQ=255)
                                       gTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGccc                                                          >  1:1797424/1‑101 (MQ=255)
                                         cGAGGTCCGCGCCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCa                                                        <  1:1934092/101‑1 (MQ=255)
                                         cGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCa                                                        <  2:3271949/101‑1 (MQ=255)
                                         cGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCa                                                        >  2:410939/1‑101 (MQ=255)
                                          gAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAg                                                       >  1:514567/1‑101 (MQ=255)
                                          gAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAg                                                       <  2:2087246/101‑1 (MQ=255)
                                          gAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAg                                                       >  2:2524022/1‑101 (MQ=255)
                                          gAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGCCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCAAGCCCCAg                                                       >  2:1292341/1‑101 (MQ=255)
                                            ggTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTc                                                     <  2:2145884/101‑1 (MQ=255)
                                              tCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCg                                                   >  1:2181838/1‑101 (MQ=255)
                                               ccGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGa                                                  <  2:1090685/101‑1 (MQ=255)
                                               ccGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGa                                                  <  1:389380/101‑1 (MQ=255)
                                                  cACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAgcg                                               >  2:2790880/1‑101 (MQ=255)
                                                  cACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAgcg                                               >  1:1821811/1‑101 (MQ=255)
                                                      ccGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAg                                           <  2:2048103/101‑1 (MQ=255)
                                                       cgggcgggCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGa                                          <  1:1799153/101‑1 (MQ=255)
                                                        gggcgggcAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGAc                                         >  2:786951/1‑101 (MQ=255)
                                                        gggcgggaAACGCAGTTATGAAATCCTGTGGATCCGTTTCCAGT.AAGCGGTCCCCCGACCAAGGATGGCGAGATGGGCGAGCCCCAGTCCGAGCGCAAGAc                                         <  2:2581732/101‑1 (MQ=255)
                                                           cgggcAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGAcccc                                      >  2:359813/1‑101 (MQ=255)
                                                           cgggcAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGAcccc                                      >  2:812926/1‑101 (MQ=255)
                                                            gggcAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGAccccc                                     >  2:344894/1‑101 (MQ=255)
                                                            gggcAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGAccccc                                     <  1:1299472/101‑1 (MQ=255)
                                                            gggcAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGAccccc                                     <  2:1950206/101‑1 (MQ=255)
                                                            gggcAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGAccccc                                     <  2:4155248/101‑1 (MQ=255)
                                                                aGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGaaa                                 >  1:2886708/1‑101 (MQ=255)
                                                                aGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGaaa                                 <  1:1079659/101‑1 (MQ=255)
                                                                 gcgcAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAg                                >  1:118112/1‑101 (MQ=255)
                                                                  cgcAGTTATGAAATCGTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAgc                               >  1:3566417/1‑101 (MQ=255)
                                                                  cgcAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAgc                               <  1:1307107/101‑1 (MQ=255)
                                                                  cgcAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGAGAGCCCCAGTCCGAGCGCTAGACCCCCGAAAgc                               >  1:3650620/1‑101 (MQ=255)
                                                                   gcAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAgcg                              <  2:3071201/101‑1 (MQ=255)
                                                                   gcAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAgcg                              >  2:656429/1‑101 (MQ=255)
                                                                      gTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAgcggtg                           >  1:2864097/1‑101 (MQ=255)
                                                                          tGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAgcggtgcggt                       >  1:938038/1‑101 (MQ=255)
                                                                          tGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAgcggtgcggg                       >  2:1550726/1‑100 (MQ=255)
                                                                           gAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGcggtgcggtg                      <  1:410990/101‑1 (MQ=255)
                                                                            aaaTCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCggtgcggtgc                     <  2:4182742/101‑1 (MQ=255)
                                                                             aaTCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGcc                    >  2:1360132/1‑101 (MQ=255)
                                                                              aTCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCa                   >  2:1080971/1‑101 (MQ=255)
                                                                                tcTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATg                 <  2:2225567/100‑1 (MQ=255)
                                                                                 cTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATgg                <  1:1033378/101‑1 (MQ=255)
                                                                                  tGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGt               >  2:3218883/1‑101 (MQ=255)
                                                                                  tGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGt               >  1:4195073/1‑101 (MQ=255)
                                                                                   gTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTc              <  2:2648757/101‑1 (MQ=255)
                                                                                      ggTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGcc           >  1:1240032/1‑101 (MQ=255)
                                                                                      ggTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGcc           >  2:3177216/1‑101 (MQ=255)
                                                                                       gTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGccc          >  1:3972180/1‑101 (MQ=255)
                                                                                       gTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGccc          <  1:3932009/101‑1 (MQ=255)
                                                                                       gTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGccc          >  1:1379353/1‑101 (MQ=255)
                                                                                       gTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGccc          >  2:2907867/1‑101 (MQ=255)
                                                                                        tCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGCCCg         >  1:1766533/1‑101 (MQ=255)
                                                                                         ccGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGCCCGt        <  2:1812362/101‑1 (MQ=255)
                                                                                          cGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGCCCGTg       <  1:423853/101‑1 (MQ=255)
                                                                                           gTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGCCCGTGc      >  1:3789010/1‑101 (MQ=255)
                                                                                             ttCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGCCCGTGCCg    <  2:3896519/101‑1 (MQ=255)
                                                                                             ttCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGCCCGTGCCg    <  2:2510440/101‑1 (MQ=255)
                                                                                              tCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGCCCGTGCCGc   >  1:2931829/1‑101 (MQ=255)
                                                                                               ccTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGCCCGTGCCGCa  <  1:656506/101‑1 (MQ=255)
                                                                                               ccTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGCCCGTGCCGCa  >  2:1662730/1‑101 (MQ=255)
                                                                                               ccTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGCCCGTGCCGCa  <  1:1330349/101‑1 (MQ=255)
                                                                                                    |                                                                                                
CTGGCCCGCGCGCTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGTAAAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGCCCGTGCCGCA  >  NC_014034/549529‑549725

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG