Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 568,802 Δ1 bp 5.5% intergenic (‑98/+68) RCAP_rcc00530 ← / ← carB hypothetical protein/carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034568,8020C→. 5.5% 18.2 ‑4.3 ‑0.3146intergenic (‑98/+68)RCAP_rcc00530/carBhypothetical protein/carbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (2/6):  ref base (97/41):  total (99/47)
Fisher's exact test strand distribution p-value = 1.39e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.89e-01

Only 100 of 201 total aligned reads displayed.

CATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGCC  >  NC_014034/568702‑568902
                                                                                                      |                                                                                                     
cATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGc                                                                                                       >  2:3543353/1‑101 (MQ=255)
 aTTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGcc                                                                                                      <  2:2292043/101‑1 (MQ=255)
 aTTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGcc                                                                                                      >  2:1977756/1‑101 (MQ=255)
 aTTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGcc                                                                                                      >  1:4122929/1‑101 (MQ=255)
 aTTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGcc                                                                                                      >  1:1776980/1‑101 (MQ=255)
    ccTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGccccc                                                                                                   >  1:4013285/1‑101 (MQ=255)
    ccTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGccccc                                                                                                   <  1:2987477/101‑1 (MQ=255)
       gCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGcccccccc                                                                                                <  1:2096656/101‑1 (MQ=255)
        ccTGCAGTTTCTGTATGTGAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGccccccccc                                                                                               <  1:1012342/101‑1 (MQ=255)
         cTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCCTCCTGgctccccccc                                                                                              >  2:3504449/1‑101 (MQ=255)
         cTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCT.GTGGCGGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGcccccccccc                                                                                              <  1:2366180/101‑1 (MQ=255)
            cAGTTTCTGTATGTGAACCGGGGCTTACGCCT.GTGGCTGGGGTGGGGCAATGGCCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGAc                                                                                           <  2:1387478/101‑1 (MQ=255)
               gttCTGTATGTTGACCGGGGCTTTCGCCTGGGGGCTG.GGTGGGGGAAAGGCCCCGCCGGGGTTTTGAAGGCCGTTGGGGC.TCCCGCCCCCCCCCCGACggg                                                                                        <  2:2151452/100‑1 (MQ=255)
                ttCTGTATGTGAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGGTGCGGCATCCTGCCCCCCCCCCCACgggg                                                                                       >  1:161210/1‑100 (MQ=255)
                ttCTGTATGTGAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGa                                                                                       >  1:3952804/1‑101 (MQ=255)
                ttCTGTATGGGAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGCCCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGa                                                                                       <  2:2349742/101‑1 (MQ=255)
                  cTGTATGTGAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGggg                                                                                     >  2:1146990/1‑101 (MQ=255)
                  cTGTATGTGAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTG.CCCCCCCCCGACGGGaggg                                                                                    >  2:1593921/1‑101 (MQ=255)
                    gTATGTGAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGCCGGGggggc                                                                                   >  2:598555/1‑101 (MQ=255)
                    gTATGTGAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGagggc                                                                                   >  1:3239632/1‑101 (MQ=255)
                     tATGTGAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGagggca                                                                                  <  2:1350582/101‑1 (MQ=255)
                     tATGTGAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGagggca                                                                                  >  1:388799/1‑101 (MQ=255)
                      ttgtgAACCGGGGCTTACGCCT.GTGGCTGGGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGagggcag                                                                                 <  2:2853812/100‑1 (MQ=255)
                      aTGTGAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGagggcag                                                                                 <  2:2719609/101‑1 (MQ=255)
                      aTGTGAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGagggcag                                                                                 <  2:874157/101‑1 (MQ=255)
                         tgAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGaggggcgggg                                                                              >  2:2380573/1‑95 (MQ=255)
                          gAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGCCGGGggggcaggggt                                                                             >  2:2367697/1‑99 (MQ=255)
                          gAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCt                                                                             <  2:452572/101‑1 (MQ=255)
                           aaCCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCCACGGGGGGGGCGGGCTg                                                                            >  2:77651/1‑101 (MQ=255)
                            acgggggCTTACGCCT.GTGGCGGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTG.CCCCCCCCCGACGGGAGGGCAGGGCTGGt                                                                          <  1:1506319/98‑1 (MQ=255)
                              cGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGGGGGCAGGGCTGGtt                                                                         >  1:1750680/1‑101 (MQ=255)
                              cGGGGCTTACGCCT.GTGGCTGCGGGGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGtt                                                                         <  2:3070802/101‑1 (MQ=255)
                              cGGGGCTCACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACCGGAGGGCAGGGCTGGtt                                                                         >  2:603125/1‑101 (MQ=255)
                               ggggCTTACGCCT.GTGGCTGGGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGttt                                                                        <  2:3849156/101‑1 (MQ=255)
                                  gCTTACGCCT.GTGGCTGCGGTGGGGCAATGCCCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCgg                                                                     <  1:2048753/101‑1 (MQ=255)
                                  gCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGGTTCgg                                                                     >  2:3945850/1‑101 (MQ=255)
                                   cTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACCGGAGGGCAGGGCTGGGTTGggg                                                                    >  2:4148940/1‑101 (MQ=255)
                                    ttACGCCT.GTGGCTGGGGTGGGGCAATGACCCCCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGggc                                                                   <  1:2983509/101‑1 (MQ=255)
                                    ttACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGGGGGCAGGGCTGGTTTCGggc                                                                   >  1:1963751/1‑101 (MQ=255)
                                    ttACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGGTTCGggc                                                                   >  1:463748/1‑101 (MQ=255)
                                    ttACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCCCCGGGGGGGCAGGGCTGGTTTCgggg                                                                   >  1:3161992/1‑100 (MQ=255)
                                     tACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGGGGGCAGGGCTGGTTTCGggcg                                                                  >  1:2908726/1‑101 (MQ=255)
                                      aCGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGCCGGGAGGGCAGGGCTGGTTTCGggcgg                                                                 >  1:2709636/1‑101 (MQ=255)
                                      aCGCCT.GTGGCGGGGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGggcgg                                                                 <  1:3263874/101‑1 (MQ=255)
                                       tccgctttgGCTGCGGTGGGGGAATGTCCCCCACGGGGTTGGGAAGG.CGCTGCGGCTTCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGggcgg                                                                 <  1:1219146/94‑1 (MQ=255)
                                        gCCT.GGGGCGGCGGTGGGGCAATGCCCCGCGCGGGCTTGGGAGGG.CCCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCa                                                               <  2:1668861/101‑1 (MQ=255)
                                         ccT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGCCGGGGGGGCAGGGCTGGGTTCGGGCGGCaa                                                              >  1:3803998/1‑101 (MQ=255)
                                          cT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCGGGGCTGGGTTTGGGGGGCAAg                                                             >  1:1922279/1‑101 (MQ=255)
                                           t.gtgGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGCCGGGGGGGCGGGGCTGGGTTCGGGCGGCAAgg                                                            >  2:2910199/1‑101 (MQ=255)
                                           t.gtgGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAgg                                                            <  2:2126767/101‑1 (MQ=255)
                                             gtgGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGgc                                                           >  1:548048/1‑101 (MQ=255)
                                              tgGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGGGGGCAGGGCTGGTTTCGGGCGGCAAgggg                                                          >  1:3273318/1‑99 (MQ=255)
                                                gCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGGGGGCAGGGCTGGGTTTGGGGGGCAAGGGGcc                                                        >  2:3175839/1‑101 (MQ=255)
                                                 cggCGGGGGGGCAATGCCCCCCACGGGTTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGccc                                                       <  1:348092/99‑1 (MQ=255)
                                                 cTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGccc                                                       >  2:3711795/1‑101 (MQ=255)
                                                 cTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGccc                                                       >  1:1465062/1‑101 (MQ=255)
                                                  tggggTGGGGCAATGACCCGCCCGGGCTTGTGAAGG.CGCTGCGGCCTCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcg                                                      <  2:505454/98‑1 (MQ=255)
                                                   gCGGTGGGGCAATGCCCCGCCCGGGCTTGGGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgc                                                     <  1:2595010/101‑1 (MQ=255)
                                                         gggCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTG.CCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcggcg.cgg                                             <  2:1402875/101‑1 (MQ=255)
                                                            cAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGcc                                           >  2:3185944/1‑101 (MQ=255)
                                                             aaTGCCCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCg                                          <  2:2564532/101‑1 (MQ=255)
                                                              aTGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGCGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.GGGACgg                                         >  1:1794472/1‑100 (MQ=255)
                                                                gACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGc                                       >  2:179568/1‑101 (MQ=255)
                                                                 ccccGCACGGGCTTGTGAAGG.CGCTGGGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGcc                                      <  1:77675/100‑1 (MQ=255)
                                                                    cGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGAGGCAAGGCGCCCGCGGCG.CGGCCGTGCCCGc                                   >  1:3026860/1‑101 (MQ=255)
                                                                     gCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCCACGGGGGGGGCGGGCTGGGTTTGGGGGGCAAGGGGCCCGCGGCG.CGGCCGTGCCCGCt                                  >  1:3685257/1‑101 (MQ=255)
                                                                       ccGGGCTTGTGAAGG.CGCTGCGGCATCCGGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGCCTGCTTa                                <  1:1977882/100‑1 (MQ=255)
                                                                       ccGGGCTTGGGAAGG.CCGTGGGGCGCCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGCCTGCTTa                                <  1:3082398/100‑1 (MQ=255)
                                                                       aCGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGGGGGCAGGGCTGGGTTCGGGCGGCAAGGCGCCCGCGGGG.CGGCCGCGCCCGCCTa                                >  2:3109284/1‑101 (MQ=255)
                                                                       aCGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGGGCCTGCttc                                >  1:1140811/1‑100 (MQ=255)
                                                                        cGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGCCTGCTTAc                               <  2:3420847/101‑1 (MQ=255)
                                                                         gggCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCCGCGGGAGGGCAGGGCTGGTTTCCGGCGGCCACGCGCCCCCGGCG.CGGGCGCGCCTCCTTACg                              >  2:946434/1‑101 (MQ=255)
                                                                          ggCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGGGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGCCCGCTTACGc                             >  2:3220457/1‑101 (MQ=255)
                                                                           gCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGCGCCTGCTTACGcc                            >  1:3540904/1‑101 (MQ=255)
                                                                           gCTTGTGAAGG.CGCTGCGGCATCCTG.CCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGCCTGCTTACGCCt                           <  1:677510/101‑1 (MQ=255)
                                                                           gCTTGTGAAGG.CGCTGCGGCATCCTG.CCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGCCTGCTTACGCCt                           <  2:3265022/101‑1 (MQ=255)
                                                                            cTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGCCTGCTTACGCCt                           <  1:598619/101‑1 (MQ=255)
                                                                             ttGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGGGCCTGCTTACGcccg                          >  2:1537615/1‑99 (MQ=255)
                                                                              tgtgAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGCCTGCTTACGCCTGc                         <  2:3289029/101‑1 (MQ=255)
                                                                              tgtgAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGGGCCTGCTTACGCCTGc                         >  2:2471339/1‑101 (MQ=255)
                                                                               gtgAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGCCTGCTTACGCCTGCa                        >  2:195811/1‑101 (MQ=255)
                                                                               gtgAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCCGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGCGCCTGCTTACGCCTGCa                        >  1:1712590/1‑101 (MQ=255)
                                                                               ggggaGG.GGCTGCGGCACCCGGCCCCCCCCCCGTCGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGCCTGCTTACGCCTGCa                        <  1:3626125/97‑1 (MQ=255)
                                                                                 gAAGG.CTCTTCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGCCTGCTTACGCCTGCAGc                      <  2:4013025/101‑1 (MQ=255)
                                                                                     g.cgcTGGGGCCTCCTCCCCCCCACCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGCCTGCTTACGCCTGCAGCGGcc                  <  2:547984/101‑1 (MQ=255)
                                                                                     g.cgcTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGCCTGCTTACGCCTGCAGCGGcc                  <  2:2983942/101‑1 (MQ=255)
                                                                                       cgcTGCGGCATCCTGCCCCCCCCCCGCCGGGGGGGGAGGGCTGGTTTCGGGCGGCAAGG.GCCCCCGGCGCCGCCCGGGCCTTCTTACCCCTCCACGGGCCg                 >  1:3322155/1‑101 (MQ=255)
                                                                                        gcTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGCCTGCTTACGCCTGCAGCGGCCgg                <  2:4122668/101‑1 (MQ=255)
                                                                                        gcTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCCCGGCG.CGGCCGTGCCTGCCTACGCCCGCCGCGCcccg                >  2:3884919/1‑99 (MQ=255)
                                                                                         cTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGCCTGCTTACGCCTGCAGCGGCCggc               >  1:3387033/1‑100 (MQ=255)
                                                                                          tGCGGCATCCTGCCCCCCCCCCGCCGGGGGGGCGGGGCTGGTTTTGGGGGGGAAGGGGCCCCCGGCG.GGGGCGGGCCTCCTTACGCCCCCGCCGGCCgggc              >  2:1555700/1‑99 (MQ=255)
                                                                                           gCGGCATCCTGCCCCCCCCCCGACGGGGGGGCAGGGCTGGTTTCGGGCGGCAAGGGGCCCGCGGCG.CGGCCGGGCCGGGTTCGCCCTGCCGCGGCCGGGcc             >  1:816945/1‑101 (MQ=255)
                                                                                            cGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGCCTGCTTCCGCCTGCAGCGGCCGGGccc            >  2:2048994/1‑100 (MQ=255)
                                                                                            cGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCg            >  2:1289775/1‑101 (MQ=255)
                                                                                                aTCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGGCCGGGAt        >  1:1842576/1‑101 (MQ=255)
                                                                                                 cccTGCCCCGCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGAtt       <  2:676758/100‑1 (MQ=255)
                                                                                                   cTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTc     >  1:576783/1‑101 (MQ=255)
                                                                                                   cTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTc     <  1:1289871/101‑1 (MQ=255)
                                                                                                      ccccccccccGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGcc  <  2:817052/101‑1 (MQ=255)
                                                                                                      ccccccccccGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGcc  <  2:314514/101‑1 (MQ=255)
                                                                                                      |                                                                                                     
CATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCT.GTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGG.CGCTGCGGCATCCTGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCG.CGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGCC  >  NC_014034/568702‑568902

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 32 ≤  ATCG  < 41 ≤  ATCG