Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014035	34,743	Δ1 bp	100%	coding (399/1041 nt)	RCAP_rcp00049 ←	iron siderophore/cobalamin ABC transporter permease

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014035	34,743	0	G→.	100.0%	139.1	62	coding (399/1041 nt)	RCAP_rcp00049	iron siderophore/cobalamin ABC transporter permease
Reads supporting (aligned to +/- strand):  new base (35/26):  ref base (0/1):  total (35/27)

GACCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATGGGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCGAGACGCCCAGCACGAAGGGTTCGGCCA  >  NC_014035/34644‑34840                                                                                                    |                                                                                                  gACCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATgg                                                                                                  >  1:76251/1‑101 (MQ=32)  ccccccGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATgga                                                                                                 <  2:2512001/100‑2 (MQ=14)  aCCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTAGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATgga                                                                                                 >  1:4131221/1‑100 (MQ=14)   cccccGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATggac                                                                                                <  2:2522516/101‑3 (MQ=11)   cccccGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATggac                                                                                                <  1:2636054/101‑3 (MQ=11)   cccccGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATggac                                                                                                <  1:1079692/101‑3 (MQ=11)                      gTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGcc                                                                            <  2:1824249/101‑1 (MQ=11)                      gTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGcc                                                                            >  2:2590207/1‑101 (MQ=11)                      gTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGcc                                                                            <  2:4130959/101‑1 (MQ=11)                       ttCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCg                                                                           <  1:921444/101‑1 (MQ=14)                         cGGCCAGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGAt                                                                         >  1:2831780/1‑101 (MQ=12)                          ggCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATg                                                                        >  1:3985729/1‑101 (MQ=17)                             ccGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCGGATGccc                                                                     <  2:874569/101‑1 (MQ=12)                              cGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGTGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCa                                                                    <  2:3488306/101‑1 (MQ=12)                              cGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCa                                                                    <  2:4000912/101‑1 (MQ=17)                               gCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAGGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAg                                                                   <  2:3305023/101‑1 (MQ=12)                                 tGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCa                                                                 >  1:793716/1‑101 (MQ=17)                                 tGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCa                                                                 >  2:3066/1‑101 (MQ=17)                                 tGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCa                                                                 >  1:3856403/1‑101 (MQ=17)                                 tGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCa                                                                 >  2:2237839/1‑101 (MQ=17)                                 tGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCa                                                                 >  1:2922106/1‑101 (MQ=17)                                  ggTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCAc                                                                <  1:1389197/101‑1 (MQ=17)                                   gTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACg                                                               <  2:172830/101‑1 (MQ=17)                                   gTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACg                                                               <  2:307047/101‑1 (MQ=17)                                    tCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGa                                                              >  1:2222266/1‑101 (MQ=17)                                    tCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGa                                                              >  2:3612347/1‑101 (MQ=17)                                      gcgcCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATg                                                            <  2:2275121/101‑1 (MQ=17)                                       cgcCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGa                                                           >  1:1429971/1‑101 (MQ=18)                                        gcCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGAc                                                          <  2:3596286/101‑1 (MQ=17)                                          cGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCg                                                        >  2:2717198/1‑101 (MQ=18)                                          cGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCg                                                        >  1:2621973/1‑101 (MQ=18)                                            ttCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGcc                                                      >  1:2285787/1‑101 (MQ=18)                                            ttCGACAAAAGCGCAACCAGCGCGAGGGCAGCGAAGGCGCCCGCGAAAGCCCTCAT.GCACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGAGGACCGcc                                                      >  2:3508964/1‑101 (MQ=11)                                             tCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCa                                                     >  2:1404500/1‑101 (MQ=18)                                             tCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCa                                                     <  2:426216/101‑1 (MQ=17)                                             tCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCa                                                     <  2:528413/101‑1 (MQ=17)                                             tCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCa                                                     >  2:621813/1‑101 (MQ=17)                                              cGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCAc                                                    >  1:4058279/1‑101 (MQ=18)                                               tctaaaaGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCAcc                                                   <  1:1625830/98‑1 (MQ=12)                                               gACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCAcc                                                   >  1:4128849/1‑101 (MQ=18)                                               gACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCAcc                                                   >  2:1490506/1‑101 (MQ=18)                                               gACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCAcc                                                   >  1:3375545/1‑101 (MQ=18)                                                 cAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACcgc                                                 <  2:323313/101‑1 (MQ=17)                                                 cAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACcgc                                                 >  2:562544/1‑101 (MQ=17)                                                  aaaaGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCAccaga                                                >  2:1625721/1‑98 (MQ=255)                                                  aaaaGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACcgcg                                                >  2:972637/1‑101 (MQ=18)                                                     aGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCg                                             >  2:1814476/1‑101 (MQ=17)                                                     aGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCg                                             <  2:1408201/101‑1 (MQ=17)                                                     aGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCg                                             >  2:3329549/1‑101 (MQ=17)                                                       cgcCACCAGCGCGAAGCCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGt                                           <  2:4009377/101‑1 (MQ=12)                                                        gcCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTc                                          <  1:1332701/101‑1 (MQ=17)                                                        gcCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTc                                          <  1:3365525/101‑1 (MQ=17)                                                          caccaGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGa                                        <  1:2666876/101‑1 (MQ=17)                                                           accaGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACGGCCCACGCGCCGGTCGAg                                       >  2:2832153/1‑101 (MQ=11)                                                           accaGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAg                                       >  1:1612604/1‑101 (MQ=17)                                                           accaGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAg                                       >  1:2214750/1‑101 (MQ=17)                                                            ccaGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAgg                                      >  2:3796634/1‑101 (MQ=18)                                                             caGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGc                                     <  1:1485863/101‑1 (MQ=17)                                                                  cgAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCccg                                >  2:1981707/1‑101 (MQ=17)                                                                  cgAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACAGCGCCGGTCGAGGCCCccg                                <  1:3328378/101‑1 (MQ=255)                                                                   gAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCccgc                               >  1:2491994/1‑101 (MQ=17)                                                                      ggCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCga                            <  2:4222002/101‑1 (MQ=17)                                                                       gCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCgag                           <  1:2431167/101‑1 (MQ=17)                                                                       gCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCgag                           >  2:1226871/1‑101 (MQ=17)                                                                        cAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCgaga                          >  1:1128958/1‑101 (MQ=14)                                                                        cAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCgaga                          <  2:1305185/101‑1 (MQ=14)                                                                        cAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCgaga                          >  2:2364233/1‑101 (MQ=14)                                                                         aGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCGAGAc                         >  1:2496826/1‑101 (MQ=11)                                                                         aGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCGAGAc                         >  2:2962294/1‑101 (MQ=11)                                                                                 cgcCCGCAAAAGCCCCCA.GGGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCCCCGCGCCGGTCGAGGCCCCCGCCGAGACGCCCAGCa                 <  2:2921887/101‑1 (MQ=255)                                                                                   cccGCCAAAGCCCCCA.GGGACAGCGACACCCCGCCCGGCCGATGCCCAGCACGATGCCCGCCCCCGCGCCGGCCGAGGCCCCCGCCGAGACGCCCAGCACg               <  1:1609920/101‑1 (MQ=11)                                                                                           aacccccAAGGGCCAGCCACGGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCGAGACGCCCAGCACGAAGGGtt        <  1:3019650/99‑1 (MQ=14)                                                                                                cccagggACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCGAGACGCCCAGCACGAAGGGTTCGGcc   <  2:161078/97‑1 (MQ=18)                                                                                                 ccagggACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCGAGACGCCCAGCACGAAGGGTTCGGCCa  <  2:3985469/98‑1 (MQ=21)                                                                                                    |                                                                                                  GACCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATGGGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCGAGACGCCCAGCACGAAGGGTTCGGCCA  >  NC_014035/34644‑34840 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG