Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,003,050 +G 2.5% coding (356/738 nt) pssA → CDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,003,0501.→G 2.5% 5.4 ‑4.0 ‑0.2158coding (356/738 nt)pssACDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (55/99):  total (59/99)
Fisher's exact test strand distribution p-value = 1.82e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.68e-01

Only 100 of 175 total aligned reads displayed.

GTCGCGCCCGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCGGCACCGGCGGGGGCGATTCTGGCGCTGCTGCCGGTC  >  NC_014034/1002950‑1003150
                                                                                                     |                                                                                                       
gTCGCGCCCGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCt                                                                                                          >  2:864428/1‑101 (MQ=255)
  cgcgCCCGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.gg                                                                                                       >  1:2493726/1‑101 (MQ=255)
     gcCCGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.ggggc                                                                                                    >  1:3906438/1‑98 (MQ=255)
     gcCCGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGccc                                                                                                    <  1:2034862/101‑1 (MQ=255)
     gcCCGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGccc                                                                                                    <  2:4015681/101‑1 (MQ=255)
      gccGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCg                                                                                                   <  2:4075763/100‑1 (MQ=255)
       ccGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.ggggcgc                                                                                                  >  2:2327976/1‑96 (MQ=255)
        cGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.ggggcggg                                                                                                 >  2:445917/1‑95 (MQ=255)
        cGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGt                                                                                                 <  2:243376/101‑1 (MQ=255)
          gggTTCTCGTCTATCTCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTc                                                                                               <  2:2101458/101‑1 (MQ=255)
          gggTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTc                                                                                               <  1:2638493/101‑1 (MQ=255)
          gggTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTc                                                                                               <  1:2019/101‑1 (MQ=255)
           ggTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCa                                                                                              <  2:2406791/101‑1 (MQ=255)
           ggTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCa                                                                                              <  2:3712538/101‑1 (MQ=255)
           ggTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCa                                                                                              <  2:156415/101‑1 (MQ=255)
            gTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTcga                                                                                             >  2:1677497/1‑99 (MQ=255)
            gTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCaa                                                                                             <  2:2669441/101‑1 (MQ=255)
             tGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAAc                                                                                            <  1:806019/101‑1 (MQ=255)
                tCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTg                                                                                         <  2:1256487/101‑1 (MQ=255)
                tCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTg                                                                                         <  1:436101/101‑1 (MQ=255)
                  gTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.Ac                                                                                      <  2:3444656/101‑1 (MQ=255)
                   tCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCTGGGCCCGGTCCacgcgcac                                                                                       >  1:952504/1‑94 (MQ=255)
                   tCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.Acc                                                                                     <  1:1957823/101‑1 (MQ=255)
                   tCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.Acc                                                                                     <  2:359107/101‑1 (MQ=255)
                    cTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCGACCGG.Accc                                                                                    >  1:1355682/1‑101 (MQ=255)
                      aTCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTCCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.gg                                                                                 <  2:3939445/101‑1 (MQ=255)
                       tCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.ggg                                                                                <  2:3887733/101‑1 (MQ=255)
                       tCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.ggg                                                                                <  1:2101356/101‑1 (MQ=255)
                         gCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGc                                                                              <  2:2838134/101‑1 (MQ=255)
                          cTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCCGGGCAACGTG.ACCC.cggggg                                                                             >  1:3262448/1‑99 (MQ=255)
                           ttCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCGACGGG.ACCC.cgggggg                                                                            >  1:2211905/1‑98 (MQ=255)
                            tCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCgag                                                                           <  1:1723798/101‑1 (MQ=255)
                                 aTGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAggg                                                                      <  2:3005812/101‑1 (MQ=255)
                                  tGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGgc                                                                     <  2:2095900/101‑1 (MQ=255)
                                      cGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCGCGGTCGACACG.GCCC.CGGGCGGGGGGGGGGCgg                                                                 >  1:1932458/1‑101 (MQ=255)
                                      cGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCgg                                                                 <  2:1480520/101‑1 (MQ=255)
                                       gAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCTGGGCCCGGTTGACCCGG.CCCC.GGGG.GGGGGGGGCGGggg                                                                >  1:2318087/1‑101 (MQ=255)
                                       gAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCggg                                                                <  1:1786435/101‑1 (MQ=255)
                                         ggggAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCGACGTG.AACC.CGGGCGCGGGGGGCGGGGGcg                                                              >  2:3471525/1‑100 (MQ=255)
                                         ggggAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCa                                                              <  1:860829/101‑1 (MQ=255)
                                          gggAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCaa                                                             <  2:640150/101‑1 (MQ=255)
                                           ggAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAg                                                            <  1:1145126/101‑1 (MQ=255)
                                            gAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCAAAGTG.ACCC.CGGGCGGGGGGGGGGCgggggaga                                                           >  2:4165062/1‑96 (MQ=255)
                                              aTGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGAcc                                                         <  1:2597441/101‑1 (MQ=255)
                                              aTGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGAcc                                                         <  2:1934110/101‑1 (MQ=255)
                                                 cgcTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCCACGCG.ACCC.GGGGCGGGGGGGGGGCGGGCGAGAcccct                                                      >  1:2587130/1‑99 (MQ=255)
                                                 cgcTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCAt                                                      <  1:1598116/101‑1 (MQ=255)
                                                   ctgggctgggTTTTCGTGCTTATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCAttt                                                    <  2:1079624/101‑1 (MQ=255)
                                                   ctgggctgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCAACCTG.ACCC.GGGGGGGGGGGGGGGGGGGGAAGGccccctt                                                    >  1:1317468/1‑97 (MQ=255)
                                                   ctgggctgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGGCAAAGTG.ACCCGGGGGGGGGGGGGGGGGGGGGAGGACCCAtt                                                     >  1:1529264/1‑101 (MQ=255)
                                                   ctgggctgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCAttt                                                    <  2:2025047/101‑1 (MQ=255)
                                                   ctgggctgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCAttt                                                    <  1:4001428/101‑1 (MQ=255)
                                                   ctgggctgggGTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCAttt                                                    <  1:452736/101‑1 (MQ=255)
                                                    tgggctgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCAACGTG.ACCC.CGGGGGGGGAGGGGGGGGGCGAGACCCACTTc                                                   >  2:3327577/1‑101 (MQ=255)
                                                     gggctgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGGCAACACG.ACCC.GGGGGGGGGAGGGGGCGGGGGAGACACacctca                                                  >  2:3070167/1‑96 (MQ=255)
                                                     gggctgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCa                                                  <  1:2490414/101‑1 (MQ=255)
                                                        ctgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCg                                               <  1:3725369/101‑1 (MQ=255)
                                                          gggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCAACGCG.ACCC.GGGGGGAGGGGGGCGGGGGGGAGACCCCATTCACCCGc                                             >  2:1320800/1‑101 (MQ=255)
                                                           ggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.g                                           <  1:403252/101‑1 (MQ=255)
                                                            gTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.Gt                                          <  1:3471777/101‑1 (MQ=255)
                                                            gTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.Gt                                          <  1:3447654/101‑1 (MQ=255)
                                                             ttttCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.Gtt                                         <  1:4092646/101‑1 (MQ=255)
                                                              tttCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCAACGTG.ACCC.CGGGCGGGGGGGGCGGGGGGAAGGCCCATTTCACCCGC.GGTc                                        >  2:2031699/1‑101 (MQ=255)
                                                               ttCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCCACGTG.ACCC.GGGGGGGGGGGGGGGGGGGCAAGAACCATTTCACCGGC.Gtttc                                       >  2:3154028/1‑99 (MQ=255)
                                                                tCGTGCTTATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCg                                      <  2:422372/101‑1 (MQ=255)
                                                                 cGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCAACGTG.ACCC.GGGGGGGGGGGGGGGGGGGGCAGACCCCCTTTACCCGCGGTTCCg                                      >  1:70796/1‑101 (MQ=255)
                                                                 cGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCgg                                     <  1:1448730/101‑1 (MQ=255)
                                                                  gTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCgga                                    <  1:676771/101‑2 (MQ=255)
                                                                  gTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCGGc                                    <  2:3810392/101‑1 (MQ=255)
                                                                  gTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCGGc                                    <  2:2549101/101‑1 (MQ=255)
                                                                  gTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCGGc                                    <  1:2945640/101‑1 (MQ=255)
                                                                    gCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTTCACGGG.GCCC.GGGGCGGGGGGGGGGCGGGGCAGACCCATTTTACCCGC.Gtttccgccc                                  >  1:2906600/1‑94 (MQ=255)
                                                                    gCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCAACCTG.ACAC.CGGGCGGGGGGGGCGGGGGCAAGACCCCTTTCTCCGGC.gggcctgccc                                  >  1:457668/1‑92 (MQ=255)
                                                                    gCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCGGCAc                                  <  2:4134994/101‑1 (MQ=255)
                                                                     cTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCGGCAcc                                 <  1:304767/101‑1 (MQ=255)
                                                                      tGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCGGCACcg                                <  2:294647/101‑1 (MQ=255)
                                                                       gATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGCTCAACGTGAACCC.GGGGGGTGG.GGGTGCCGGGCAGAGCCACTTTTACGGC.GTTTCCGCACcgc                               >  1:51085/1‑100 (MQ=255)
                                                                          cTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCCACGTG.ACCC.GGGGCGGGGGGGGCGGGGGCAAGACCCCATTCACCCGC.GTTCCCGCCCccgcgg                            >  2:1706246/1‑101 (MQ=255)
                                                                          cTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGGTCAACGCG.ACCC.CGGGCGGGGGGGGGGCGGGGAAGACCCATTTTACCGGC.GCTCCCGCCCcggcgg                            >  2:3257496/1‑101 (MQ=255)
                                                                           tATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCAACGGG.ACCC.CGGGCGGGGGGGGCGCGGGGAAGACCCCTTTCACCCGC.GTTTCGGCCCCCGCggg                           >  1:767631/1‑101 (MQ=255)
                                                                             tGCCGCCTCGGCCTGTCTGCGGCTGGGGGGGGTTGACCTTG.A.CC.GGGGGGGGGAGGGGGGGGGCCGGACCCGATTCACCGGG.GGTGCCGTCCCGGCggggg                         >  1:1379792/1‑101 (MQ=255)
                                                                                 gccTCGGCCTGGCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCGGCACCGGCGGGGGCGAt                     <  2:4070664/101‑1 (MQ=255)
                                                                                     cGGCCTGTCTGCGGCT.GGGCCGGGTCAACGTG.ACCC.GGGGCGGGGGGGGGGCGGGGAAGACCCCTTTCACCCGC.GGTCCCGCGCCGGCgggggggggtgct                 >  2:622711/1‑93 (MQ=255)
                                                                                     cGGCCTGTCTGCGGCT.GGGCCGGGTCAAAGTG.ACCC.CGGGGGAGGAGGGCGCGGGCAAGACCCACTTCACCCGC.GTTCCGGGACCGGCgggggggggtctg                 >  2:719814/1‑93 (MQ=255)
                                                                                     cGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCGGCACCGGCGGGGGCGATTCTg                 <  1:2705169/101‑1 (MQ=255)
                                                                                       gCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCGGCACCGGCGGGGGCGATTCTGgc               <  1:1038627/101‑1 (MQ=255)
                                                                                       gCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.CGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GGTCCCGCCCCCGCGGGGGGGGGTCTggg               >  2:3683037/1‑100 (MQ=255)
                                                                                         cTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCGGCACCGGCGGGGGCGATTCTGgcgc             <  2:952419/101‑1 (MQ=255)
                                                                                          tGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCGGCACCGGCGGGGGCGATTCTGGCgct            <  1:3752815/101‑1 (MQ=255)
                                                                                          tGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCGGCACCGGCGGGGGCGATTCTGGCgct            <  1:2880476/101‑1 (MQ=255)
                                                                                           gTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCGGCACCGGCGGGGGCGATTCTGGCgctg           <  2:522019/101‑1 (MQ=255)
                                                                                           gTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCGGCACCGGCGGGGGCGATTCTGGCgctg           <  1:1677612/101‑1 (MQ=255)
                                                                                             cTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCGGCACCGGCGGGGGCGATTCTGGCgctgct         <  2:2296664/101‑1 (MQ=255)
                                                                                             cTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCGGCACCGGCGGGGGCGATTCTGGCgctgct         <  1:622775/101‑1 (MQ=255)
                                                                                               gCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCGGCACCGGCGGGGGCGATTCTGGCGCtgctgc       <  1:660493/101‑1 (MQ=255)
                                                                                                cgtcT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCGGCACCGGCGGGGGCGATTCTGGCGCTGCTGcc      <  2:3563749/98‑1 (MQ=255)
                                                                                                cGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCGGCACCGGCGGGGGCGATTCTGGCGCTGCTGcc      <  2:3025272/101‑1 (MQ=255)
                                                                                                cGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCGGCACCGGCGGGGGCGATTCTGGCGCTGCTGcc      <  1:4215996/101‑1 (MQ=255)
                                                                                                cGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCGGCACCGGCGGGGGCGATTCTGGCGCTGCTGcc      <  1:3395759/101‑1 (MQ=255)
                                                                                                   cT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCGGCACCGGCGGGGGCGAGTCTGGCGCTGCTGCCCGt   >  2:1914005/1‑101 (MQ=255)
                                                                                                     |                                                                                                       
GTCGCGCCCGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTG.ACCC.GGGGCGAGGAGGGCGCGGGCAAGACCCATTTCACCGGC.GTTCCGGCACCGGCGGGGGCGATTCTGGCGCTGCTGCCGGTC  >  NC_014034/1002950‑1003150

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG