Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,278,283 +G 2.7% coding (456/1785 nt) RCAP_rcc01204 ← peptidoglycan binding domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,278,2831.→G 2.7% 2.5 ‑3.3 ‑0.1112coding (456/1785 nt)RCAP_rcc01204peptidoglycan binding domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (35/74):  total (38/74)
Fisher's exact test strand distribution p-value = 3.70e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.36e-01

Only 100 of 153 total aligned reads displayed.

TGTAAACGCCCACCCATTCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGTTTCGGCCGCTTCCGGCGGCCAGAACTGCG  >  NC_014034/1278183‑1278383
                                                                                                     |                                                                                                            
tgtAAACGCCCACCCATTCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACcggc                                                                                                               <  2:1947174/101‑1 (MQ=255)
tgtAAACGCCCACCCATTCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACcggc                                                                                                               >  2:3060926/1‑101 (MQ=255)
  tAAACGCCCACCCATTCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACcggc.gg                                                                                                            <  1:3493238/101‑1 (MQ=255)
  tAAACGCCCACCCATTCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACcggc.gg                                                                                                            >  1:3015563/1‑101 (MQ=255)
   aaaCGCCCACCCATTCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCTTCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACcggc.ggc                                                                                                           >  2:2367296/1‑100 (MQ=255)
   aaaCGCCCACCCATTCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.ggg                                                                                                           >  2:2870617/1‑101 (MQ=255)
   aaaCGCCCACCCATTCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGCCTCGACCGGC.ggg                                                                                                           >  2:3036288/1‑101 (MQ=255)
     aCGCCCACCCATTCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.ggggg                                                                                                         >  1:3934419/1‑101 (MQ=255)
         ccacccaTTCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.ggggggggg                                                                                                     >  2:2939013/1‑97 (MQ=255)
         ccacccaTTCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGcc                                                                                                     <  1:3435001/101‑1 (MQ=255)
          cacccaTTCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.ggggggggcc                                                                                                    >  2:2522587/1‑96 (MQ=255)
          cacccaTTCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCa                                                                                                    <  1:1066362/101‑1 (MQ=255)
          cacccaTTCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCa                                                                                                    <  1:976212/101‑1 (MQ=255)
           acccaTTCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.ggggggggccg                                                                                                   >  2:325536/1‑95 (MQ=255)
            cccaTTCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGGGcccgg                                                                                                  >  1:3984168/1‑98 (MQ=255)
            cccaTTCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGGGccccc                                                                                                  >  1:326522/1‑98 (MQ=255)
            cccaTTCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGt                                                                                                  <  2:3605181/101‑1 (MQ=255)
            cccaTTCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCC.CCGGCTCCTCCGAGCCGGGGGGGTCGTCCAGGGTCTCGACCCGC.gggggggggcgga                                                                                                 >  1:3701989/1‑93 (MQ=255)
             ccaTTCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.ggggggggggggg                                                                                                 >  2:1535010/1‑93 (MQ=255)
             ccaTTCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGGGCCCGtt                                                                                                 >  2:4235642/1‑101 (MQ=255)
             ccaTTCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGtt                                                                                                 <  1:2870057/101‑1 (MQ=255)
             ccaTTCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGtt                                                                                                 <  1:781097/101‑1 (MQ=255)
              caTTCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTc                                                                                                <  2:1781345/101‑1 (MQ=255)
                ttCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGGGcccggcggg                                                                                              >  2:3211979/1‑94 (MQ=255)
                  ccAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGGGCCAGGTCg.ggg                                                                                           >  2:1462168/1‑100 (MQ=255)
                  ccAGGGCTTCCTGAATCTTCATCCGCCCCTCGCCGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGGGCCAggggcggc                                                                                            >  2:3389901/1‑94 (MQ=255)
                    aGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGGGCCCGGTCG.Ggggc                                                                                         >  1:385285/1‑101 (MQ=255)
                    aGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.Ggcgc                                                                                         <  1:863894/101‑1 (MQ=255)
                      ggCTTCCTGATTCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCg                                                                                       <  2:2292412/101‑1 (MQ=255)
                         ttCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGGGGCCGCCCG.GGCGGCgggc                                                                                    >  1:786467/1‑99 (MQ=255)
                         ttCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.tt                                                                                   <  2:466530/101‑1 (MQ=255)
                           ccTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCATGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTcc                                                                                 <  2:352537/101‑1 (MQ=255)
                           ccTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGGGGCCGGCCG.GGGGCCggggcc                                                                                  >  1:4003818/1‑97 (MQ=255)
                            cTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGGGCCCGTTCG.GGCGCCCG.GTccc                                                                                >  2:3649871/1‑100 (MQ=255)
                             tGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCcgc                                                                               <  1:470380/101‑1 (MQ=255)
                             tGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCcgc                                                                               <  2:3027347/101‑1 (MQ=255)
                                aTCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGGGCCCGTTCG.GGGGCCGG.GTCCGCggg                                                                            >  2:1289894/1‑100 (MQ=255)
                                aTCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGt                                                                            <  1:2522771/101‑1 (MQ=255)
                                 tCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.c                                                                          <  1:3556763/101‑1 (MQ=255)
                                  cTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.g                                                                        <  2:3123144/101‑1 (MQ=255)
                                   ctcgtCCGCCCCTCGCGGCCCAGCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.gg                                                                       <  2:2153036/97‑1 (MQ=255)
                                     cATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.cc                                                                    <  1:939848/101‑1 (MQ=255)
                                      aTCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGGGGCAGGTCG.GGGCGCGG.GTGCGCGGG.C.GG.gcc                                                                   >  2:906413/1‑101 (MQ=255)
                                            cccTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGGGGCCGGTCG.GGCGCCCG.GTCCGCCGG.C.GG.CCCGGgcgg                                                             >  1:1757969/1‑100 (MQ=255)
                                              cTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCc..g                                                         <  2:1504595/101‑1 (MQ=255)
                                               tCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCc..gg                                                        <  1:2283613/101‑1 (MQ=255)
                                               tCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCc..gg                                                        <  1:244356/101‑1 (MQ=255)
                                                 gcgGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGGGGCGGGGCG.CGCGCCGG.CTTCCCGGC.C.CG.GCCGGGCCCc..gggg                                                      >  1:4002247/1‑101 (MQ=255)
                                                  cgGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGGGCCCCTTCG.GGGGCCGG.GTCCCCGGG.CGGG.CCCGGGGCCc..gggg                                                      >  1:3964638/1‑101 (MQ=255)
                                                   ggCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCCGTTCG.GGCGCCGG.GTCCGCGGT.C.GG.CCCGGGCGCc..ggggc.g                                                   >  2:3163612/1‑101 (MQ=255)
                                                   ggCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCc..ggggc.g                                                   <  1:1522074/101‑1 (MQ=255)
                                                   ggCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGGTCG.GGCGCCGG.GTCCGCGGG.C.GG.CCCGGGCGCc..cgggc.g                                                   >  1:614953/1‑101 (MQ=255)
                                                     cccATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCc..ggggc.ggg                                                 <  1:1242687/101‑1 (MQ=255)
                                                       cATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.ggggg                                               <  1:2121897/101‑1 (MQ=255)
                                                         tCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAg                                             <  2:2442446/101‑1 (MQ=255)
                                                         tCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAg                                             <  2:1988101/101‑1 (MQ=255)
                                                           gCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAggg                                           <  2:3834982/101‑1 (MQ=255)
                                                           gCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAggg                                           <  1:2703408/101‑1 (MQ=255)
                                                             ctcctcGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGGGGCCGGTCG.GGGGGCGG.GTGCGCCGG.C.GG.CCCCGGGGCC..CGGGG.GGGGGGggggg                                         >  1:1128291/1‑99 (MQ=255)
                                                             ctcctcGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGgcg                                         <  1:2612280/101‑1 (MQ=255)
                                                               cctcGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGcgcg                                       <  1:3695473/101‑1 (MQ=255)
                                                               cctcGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGcgcg                                       <  2:3331394/101‑1 (MQ=255)
                                                                 tcGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGc                                     <  2:326792/101‑1 (MQ=255)
                                                                    gcgcggcgcgCATCGTCCAGGGTCTCGACCGGCGGGGGGGGCGCGTCGG.GGGGCGGG.GTCGGCGG..C.GG.GCCGGGGGCG..CGGGC.GGGGGGGGGGGGGGGCgg                                  >  1:1530488/1‑101 (MQ=255)
                                                                     cgcggcgcgCATCGTCCAGGGTCTCGACCGGC.GGGGGGGGCCGGTCG.GGGGCCGG.GGCCCCGGG.C.GG.CCCCGGGGCC..GGGGG.GGGGGGGGGGGGGGGCggg                                 >  1:1982992/1‑101 (MQ=255)
                                                                      gcgctgcgcATCGTCCAGGGTCTCGACCGGC.GGGGGGGGCCCAGCC.GGCGCCGG.GGCCGCGGTCC.GG.CCCCGCCGCC..CGGGC.CGGGG.GGGCGGGGGGgggg                                >  2:1413957/6‑101 (MQ=255)
                                                                        ggcgcgCATCGTCCAGGGTCTCGACCGGC.GGGGGGGCCCGGGCG.GGGGGCGG.GTTCGCGGG.C.GGCCCCGGGGGCG..GGGGG.GGGGGGGGGGGGGGGCggggg                               >  2:2561334/1‑100 (MQ=255)
                                                                         gcgcgcATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGttt                             <  2:78078/101‑1 (MQ=255)
                                                                         gcgcgcATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGttt                             <  1:2246618/101‑1 (MQ=255)
                                                                          cgcgcATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGTTTc                            <  1:325581/101‑1 (MQ=255)
                                                                          cgcgcATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGTTTc                            <  2:3983908/101‑1 (MQ=255)
                                                                           gcgcATCGTCCAGGGTCTCGACCGGC.GGGGGGGGCCGTTTG.GGGGCCGG.GTCCGCGGC.G.GG.CCCCGGCGCCCGGGGGG.GGGGGGGGGCGGGGCCgggggtg                             >  2:273833/1‑98 (MQ=255)
                                                                             gcATCGTCCAGGGTCTCGACCGGCGGGGGGGGCCGGCGGG.GGCCCCGC.GCCCGGGGG.G.GG.CGCGGCGGCG..GGGGG.GGGGGGGGGGGGGGCCggggcggggg                          >  1:2005128/1‑95 (MQ=255)
                                                                             gcATCGTCCAGGGTCTCGACCGGC.GGGGGGGCCGGAGCGCGGGCCCGG.TTCTCCGG..G.GG.GCCGGGCGCC..GGGGC.GGGGGGGGGGGGCGCCGGGGGGTCGGc                         >  2:4084298/1‑101 (MQ=255)
                                                                               aTCGTCCAGGGTCTCGACCGGC.GGGGGGGGCCGGTCG.GGGGGCCG.GTCCGCGGG.CGGG.CCCGGGGGCC..GGGGG.GGGGGGGGGGGGGGCGgggggtgggggg                        >  2:3436841/1‑93 (MQ=255)
                                                                                 cGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGTTTCGGCCGCt                     <  2:4111683/101‑1 (MQ=255)
                                                                                   tCCAGGGTCTCGACCGGC.GGGGGCGCCCGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGTTTCGGCCGCTTc                   <  2:355003/101‑1 (MQ=255)
                                                                                   tCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTTG.GGCGCCGG.TTCCGCGGC.C.GG.CCCGGGGGCC..CGGGC.GGGGGGGGGGGCGGGCGGGGGTTCGGCCggcgc                   >  2:3747422/1‑97 (MQ=255)
                                                                                   tCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGGGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGTTTCGGCCGCTTc                   <  2:1757850/101‑1 (MQ=255)
                                                                                   tCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGTGGTTTCGGCCGCTTc                   <  1:3565162/101‑1 (MQ=255)
                                                                                    ccAGGGTCTCGACCGGC.GGGGGCGCCATTTTC.GGGGCGGGCCTCCCCGG..C.CG.CGCCGCCGAC..GGGGCGGGGGGGGGGGGGGGCGGGGGTTTCGGGGGCTTc                   >  2:2689131/1‑101 (MQ=255)
                                                                                     cAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGTTTCGGCCGCTTCcg                 <  2:971089/101‑1 (MQ=255)
                                                                                     cAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGTTTCGGCCGCTTCcg                 <  2:3297537/101‑1 (MQ=255)
                                                                                      aGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGTTTCGGCCGCTTCcgg                <  1:2870836/101‑1 (MQ=255)
                                                                                       gggTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGTTTCGGCCGCTTCcggc               <  1:1746203/101‑1 (MQ=255)
                                                                                           ctggagcggc.ggGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGTGCGGCCGGGGTTTCGGCCGCTTCCGGCGGcc           <  1:3747676/95‑1 (MQ=255)
                                                                                           ctcGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGTTTCGGCCGCTTCCGGCGGcc           <  1:1462270/101‑1 (MQ=255)
                                                                                            tggACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGTTTCGGCCGCTTCCGGCGGCCa          <  2:3949293/99‑1 (MQ=255)
                                                                                            tcGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGTTTCGGCCGCTTCCGGCGGCCa          <  1:2715389/101‑1 (MQ=255)
                                                                                            tcGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGTTTCGGCCGCTTCCGGCGGCCa          <  1:544683/101‑1 (MQ=255)
                                                                                               gccGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGTTTCGGCCGCTTCCGGCGGCCAGaa       <  1:894456/100‑1 (MQ=255)
                                                                                               aCCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGTTTCGGCCGCTTCCGGCGGCCAGaa       <  1:3049575/101‑1 (MQ=255)
                                                                                                ccGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGTTTCGGCCGCTTCCGGCGGCCAGAAc      <  2:2914147/101‑1 (MQ=255)
                                                                                                ccGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGTTTCGGCCGCTTCCGGCGGCCAGAAc      <  1:1424903/101‑1 (MQ=255)
                                                                                                  ggc.nnGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGTTTCGGCCGCTTCCGGCGGCCAGAACTg    <  1:4235904/101‑1 (MQ=255)
                                                                                                  ggc.ggGGGCGCCAGTTTG.GGCGCCGG.TTCCCCGGT.C.CG.GCCGGGCGCC..GGGGC.GGGGGAGGGCGGGGGCGGGGGTTCCGGGGCGTCccgcggggaggaacg    >  2:620355/1‑92 (MQ=255)
                                                                                                  ggc.ggGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGTTTCGGCCGCTTCCGGCGGCCAGAACTg    <  2:3669899/101‑1 (MQ=255)
                                                                                                  ggc.ggGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGTTTCGGCCGCTTCCGGCGGCCAGAACTg    <  1:2084975/101‑1 (MQ=255)
                                                                                                   gc.ggGGACGCCAGTTCG.GGCCCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGTTTCGGCCGCTTCCGGCGGCCAAAACTgc   <  2:847321/101‑1 (MQ=255)
                                                                                                    c.ggGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGTTTCGGCCGCTTCCGGCGGCCAGAACTgcg  <  1:1289990/101‑1 (MQ=255)
                                                                                                     |                                                                                                            
TGTAAACGCCCACCCATTCCAGGGCTTCCTGAATCTTCATCCGCCCCTCGCGGCCCATCGCCTCCTCGGCGCGGCGCGCATCGTCCAGGGTCTCGACCGGC.GGGGGCGCCAGTTCG.GGCGCCGG.TTCCGCGGT.C.GG.CCCGGGCGCC..GGGGC.GGGGGAGGGCGCGGCCGGGGTTTCGGCCGCTTCCGGCGGCCAGAACTGCG  >  NC_014034/1278183‑1278383

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG