Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,286,746 +G 2.9% coding (527/912 nt) folB → dihydroneopterin aldolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,286,7461.→G 2.9% 5.0 ‑3.9 ‑0.6137coding (527/912 nt)folBdihydroneopterin aldolase
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (48/85):  total (52/85)
Fisher's exact test strand distribution p-value = 1.93e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.40e-01

Only 100 of 184 total aligned reads displayed.

GTGAAGGCGCTTGATGACGAGGCGACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGCCCCCGCCCGAGACCGGGCACCGGCCGACGCAGCGGCGCATCGATCTGCTC  >  NC_014034/1286646‑1286846
                                                                                                      |                                                                                                     
 tGAAGGCGCTTGATGACGAGGCGACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.g                                                                                                      >  2:105716/1‑101 (MQ=255)
  gAAGGCGCTTGATGACGAGGCGACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.gg                                                                                                     <  1:761333/101‑1 (MQ=255)
   aaGGCGCTTGATGACGAGGCGACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.ggc                                                                                                    <  2:1420875/101‑1 (MQ=255)
     ggCGCTTGATGACGAGGCGACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.ggggg                                                                                                  >  1:1049244/1‑101 (MQ=255)
       cgcTTGATGACGAGGCGACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGcc                                                                                                <  2:1500578/101‑1 (MQ=255)
       cgcTTGATGACGAGGCGACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCCG.CT.tgcggcg                                                                                                >  2:584486/1‑100 (MQ=255)
        gcTTGATGACGAGGCGACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.ggggggcc                                                                                               >  1:3063631/1‑98 (MQ=255)
        gcTTGATGACGAGGCGACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCt                                                                                               <  1:2040892/101‑1 (MQ=255)
         cTTGATGACGAGGCGACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTc                                                                                              <  1:2234066/101‑1 (MQ=255)
          ttGATGACGAGGCGACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGGCCG.GGCGGcccc                                                                                              >  1:1480914/1‑99 (MQ=255)
          ttGATGACGAGGCGACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGGGGGCtgc                                                                                             >  2:947763/1‑99 (MQ=255)
           tgatgaCGAGGCGACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGGGGcccccg                                                                                            >  2:4186251/1‑97 (MQ=255)
            gatgaCGATGCGACCCTGCATCCGGTCGTGGCCGTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCggg                                                                                           <  1:3653032/101‑1 (MQ=255)
            gatgaCGAGGCGACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCggg                                                                                           <  2:4005695/101‑1 (MQ=255)
             atgaCGAGGCGACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCgggg                                                                                          <  1:3532669/101‑1 (MQ=255)
               gaCGAGGCGACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGGGGGCGCcggggg                                                                                        >  2:3773729/1‑100 (MQ=255)
                aCGAGGCGACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGTGgcg                                                                                       <  2:3579242/101‑1 (MQ=255)
                aCGAGGCGACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCCCcgggggg                                                                                       >  2:2536431/1‑99 (MQ=255)
                  gAGGCGACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGcc                                                                                     <  2:856870/101‑1 (MQ=255)
                   gggCGACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCg                                                                                    <  2:3698019/100‑1 (MQ=255)
                   aGGCGACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGGGGGCCCCGGGGGGCCg                                                                                    >  2:2641368/1‑101 (MQ=255)
                   aGGCGACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCg                                                                                    <  1:3387009/101‑1 (MQ=255)
                    ggCGACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CTGGGGGGCCCCGGGGGGGCCg                                                                                    >  1:3815803/1‑101 (MQ=255)
                     gCGACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGGGGGCCCggggggggccgg                                                                                  >  2:2966014/1‑94 (MQ=255)
                       gACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGa                                                                                <  1:2254972/101‑1 (MQ=255)
                         cGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGAtt                                                                              <  1:1641103/101‑1 (MQ=255)
                         cGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGAtt                                                                              <  1:1446757/101‑1 (MQ=255)
                          gCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTc                                                                             <  1:3007516/101‑1 (MQ=255)
                            tGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGGGGGCCCGGGGGGGGCgggggttttg                                                                           >  1:1283450/1‑93 (MQ=255)
                            tGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGGGGGCCCCGGGGGGGCGGCGGTTCTg                                                                           >  2:290740/1‑101 (MQ=255)
                            tGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGGGGGCCCCGGGGGGCCCGGGGTTCtt                                                                           >  1:315391/1‑100 (MQ=255)
                             gCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTgg                                                                          <  1:2502707/101‑1 (MQ=255)
                             gCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTgg                                                                          <  1:320516/101‑1 (MQ=255)
                                tCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGGGGCCCCGGGGGGGCCGGGGGTTTTGGGgg                                                                       >  1:609982/1‑101 (MQ=255)
                                 ccGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGGGGGCCCGCGGGGGCCCGGGGTTCTTGCggg                                                                      >  2:1014463/1‑100 (MQ=255)
                                 ccGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGGCTCCGGGGGGGCGGGGGTTTTGGGggg                                                                      >  2:3913088/1‑100 (MQ=255)
                                  cGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTg                                                                     <  2:24201/101‑1 (MQ=255)
                                  cGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTg                                                                     <  2:2112296/101‑1 (MQ=255)
                                   ggTGGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTgg                                                                    <  2:1539408/101‑1 (MQ=255)
                                   ggTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTgg                                                                    <  1:2931821/101‑1 (MQ=255)
                                     tCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGGGGGCCCGGGGGCGCCCGGGGTTCTTGCGGGgggg                                                                  >  1:3699168/1‑100 (MQ=255)
                                     tCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGGCCCGGGGGGGCCGGGGGTTCTGTCGGGgggg                                                                  >  2:1057994/1‑100 (MQ=255)
                                     tCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGc                                                                  <  1:1511318/101‑1 (MQ=255)
                                      cGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.a                                                                <  1:3444436/101‑1 (MQ=255)
                                      cGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.a                                                                <  2:2362081/101‑1 (MQ=255)
                                      cGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.a                                                                <  2:322352/101‑1 (MQ=255)
                                       gTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGGCCCGGGGGGGCCCGGGGTTTTGGCGGGgggggt                                                                >  2:1333960/1‑98 (MQ=255)
                                            cTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGGGGCCTCCGGGGGGCCCGGGGTTCTGCCGGGGGGC.CTGcccc                                                          >  1:1835562/1‑100 (MQ=255)
                                             ttCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCccgc                                                         <  1:3228220/101‑1 (MQ=255)
                                              tCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGGGCCGGGGATTCTGGGGGGggggggggccccc                                                         >  2:1811739/1‑91 (MQ=255)
                                                 tctcGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTg                                                     <  2:1963907/101‑1 (MQ=255)
                                                 tctcGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTg                                                     <  2:1913853/101‑1 (MQ=255)
                                                  ctcGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGGCCCCGGGGCGCCGGGGGTTCTTGCGGGGGGC.ATGGCCCCCCTGc                                                    >  2:163811/1‑101 (MQ=255)
                                                    cGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGccc                                                  <  2:21893/101‑1 (MQ=255)
                                                    cGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGccc                                                  <  2:465863/101‑1 (MQ=255)
                                                     gAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGGGGGCCCGGGGGGGCCGGGGGTTTTTGCGGGGGGG.CTGGCCGCCCCGcccc                                                 >  2:2226637/1‑101 (MQ=255)
                                                     gAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGcccc                                                 <  2:3353157/101‑1 (MQ=255)
                                                     gAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGcccc                                                 <  1:2222994/101‑1 (MQ=255)
                                                     gAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGcccc                                                 <  2:627351/101‑1 (MQ=255)
                                                      aaTGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGGGCCCGGGGTTCTGGCGGGGGGG.ATGGCACCCGTGccccc                                                >  2:521259/1‑101 (MQ=255)
                                                      aaTGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGccccc                                                <  1:2536622/101‑1 (MQ=255)
                                                      aaTGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGccccc                                                <  2:278155/101‑1 (MQ=255)
                                                      aaTGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCGCGGGGCCGCCGGGGATTCTGGCGGGGGGG.ACGGCCGCCGCGgcccc                                                >  1:3790519/1‑101 (MQ=255)
                                                       aTGACGTCATCGCGGCTCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGCCcccg                                               <  1:2791458/101‑1 (MQ=255)
                                                       aTGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGGGGCCCCGGGGGGGCCCGGGGTTTTGGCGGGGGGG.ATGCCCCCCGCGcccccc                                               >  1:1538343/1‑100 (MQ=255)
                                                       aTGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGCCcccg                                               <  1:2679186/101‑1 (MQ=255)
                                                        tGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGGGGCCCCGGGGGGGCCGGGGGTTCTTGCGGGGGGG.ATGCCCCCCCCGccccccc                                              >  1:3981083/1‑99 (MQ=255)
                                                           cGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGGGGCCCCGGGGGCGCCGGGGGTTCTTGCGGGGGGG.CTGCCCGCCGTGCCcccccccc                                           >  1:833219/1‑100 (MQ=255)
                                                           cGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGCCcccgcccg                                           <  1:2641574/101‑1 (MQ=255)
                                                             tCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGGGGCCTCGGGGGGGCCGGGGGTTTTGGCGGGGGGG.ATGCCCGCCGGGCCCCCCCCCgag                                         >  1:2448310/1‑101 (MQ=255)
                                                              cATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGGGGCCTCGGGGGCGCCGGGGATTTTTGCGGGGGGG.GCGCCCGCCGGGCCCCCCCCCcaga                                        >  1:1497758/1‑101 (MQ=255)
                                                               aTCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGGGGGCCCCGGGGGGCCGGCGGTTCTTGGGGGGGGCTTTGCCCACC.TGCCcccgcccccgac                                       >  2:4165336/1‑96 (MQ=255)
                                                               aTCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCCCGGGGGGGCCGGGGATTCTGGCGGGGGGC.ATGCCCGCCGTGCCcccccccccgac                                       >  1:4204160/1‑96 (MQ=255)
                                                                gcgcgGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGCCCCCGCCCGAGAcc                                      <  2:3321620/100‑1 (MQ=255)
                                                                 cgcgGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGGGGCCCCGGGGGCGCCGGGGGTTCTGGGGGGGGGC.ATGCCCCCCGGGCCCCCGCCCCAGACCg                                     >  1:1761901/1‑101 (MQ=255)
                                                                 cgcgGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGCCCCCGCCCGAGACCg                                     <  1:480087/101‑1 (MQ=255)
                                                                    ggcgCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGGGGCCCCGGGGGGGCCGGGGATTCTGGCGGGGGGC.ATGCCCGCCGTGCCCCCCCCCGCGACCGGGc                                  >  1:2053862/1‑101 (MQ=255)
                                                                    ggcgCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGGGGCCCCGGGGGCGCCGGGGGTTCTGGGGGGGAGG.ATGCCCGCCGTGCCCCCCCCCCCGACCCGGc                                  >  1:3604501/1‑101 (MQ=255)
                                                                     gcgcCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGGGGCCTCGGGGGGGCCGGGGATTCTGGCGGTGGGC.ATGCCCGCCGTGCCCCCGCCCCAGACCGGGcc                                 >  1:1340367/1‑100 (MQ=255)
                                                                      cgcCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGCCCCCGCCCGAGACCGGGCAc                                <  2:2126782/101‑1 (MQ=255)
                                                                        ccGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCCCGGGGGGGCCGGGGATTTTGGCGGGGGGC.ATGCCCGCCGTGCCCCCGCCCGAGACCGGGCACCg                              >  1:3266109/1‑101 (MQ=255)
                                                                         cGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCGGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGCCCCCGCCCGAGACCGGGCAccag                             <  2:3492159/101‑3 (MQ=255)
                                                                           gATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGGGCCGGGGATTCTGGCGGTGGGC.ATGCCCGCCGTGCCCCCCCCCCAGACCGGGCACCGGcc                           >  1:669380/1‑101 (MQ=255)
                                                                            aTCTGGCGGCGCGGCTTGACCGG.CT.GGCGGGCTCGGGGGCCGCGGCGATTCTGGCGGGGGGG.ACGCCCGCCGTGCCCCCGCCCGGGCCCGGGCCCCGGCCg                          >  2:2095506/1‑101 (MQ=255)
                                                                            aTCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGCCCCCGCCCGAGACCGGGCACCGGCCg                          <  2:977073/101‑1 (MQ=255)
                                                                            aTCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGCCCCCGCCCGAGACCGGGCACCGGCCg                          <  1:1739199/101‑1 (MQ=255)
                                                                              cTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGCCCCCGCCCGAGACCGGGCACCGGCCGAc                        <  2:3946151/101‑1 (MQ=255)
                                                                                ggcggcGCGGCTTGACCGG.CT.GGCGGCCTCCGGGGGGCCGGGGATTCTGGCGGTGGGG.ATCCCCCCCGTGCCCCCGCCCGCGACCGGGCACCGGCCgcccc                      >  2:1350732/1‑97 (MQ=255)
                                                                                 gcggcgcggcTTGACCGG.CT.GGCGGCCTCGGGGGGGCCGGGGGTTCTGGGGGTGGGC.ATGCCCGCCCGGCCCCCGCCCGAGGCCCGGCACCGGCCCACGcc                     >  1:1063826/1‑100 (MQ=255)
                                                                                  cggcgcggcTTGCCCGG.CG.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGCCCCCGCCCGAGACCGGGCACCGGCCGACGCAg                    <  1:2912246/101‑1 (MQ=255)
                                                                                   ggcgcggcTTGCCCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGCCCCCGCCCGAGACCGGGCACCGGCCGACGCAgg                   <  2:1193955/101‑2 (MQ=255)
                                                                                   ggcgcggcTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGCCCCCGCCCGAGACCGGGCACCGGCCGACGCAgc                   <  1:1039793/101‑1 (MQ=255)
                                                                                    gcgcggcTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGCCCCCGCCCGAGACCGGGCACCGGCCGACGCAgcg                  <  1:3052321/101‑1 (MQ=255)
                                                                                        ggcTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGCCCCCGCCCGAGACCGGGCACCGGCCGACGCAGCGgcgc              <  1:192398/101‑1 (MQ=255)
                                                                                          cTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGCCCCCGCCCGAGACCGGGCACCGGCCGACGCAGCGGCGCAt            >  1:2702260/1‑101 (MQ=255)
                                                                                           ttGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGCCCCCGCCCGAGACCGGGCACCGGCCGACGCAGCGGCGCATc           >  1:1986224/1‑101 (MQ=255)
                                                                                               ccGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGCCCCCGCCCGAGACCGGGCACCGGCCGACGCAGCGGCGCATCGATc       >  1:181947/1‑101 (MQ=255)
                                                                                                cGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGCCCCCGCCCGAGACCGGGCACCGGCCGACGCAGCGGCGCATCGATCt      <  1:2684175/101‑1 (MQ=255)
                                                                                                cGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGCCCCCGCCCGAGACCGGGCACCGGCCGACGCAGCGGCGCATCGATCt      >  1:1174912/1‑101 (MQ=255)
                                                                                                cGG.CG.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGCCCCCGCCCGAGACCGGGCACCGGCCGACGCAGCGGCGCATCGAtat      <  2:778675/101‑3 (MQ=255)
                                                                                                      |                                                                                                     
GTGAAGGCGCTTGATGACGAGGCGACGCTGCATCCGGTCGTGGCCTTCCTCTCGAATGACGTCATCGCGGCGCCGGATCTGGCGGCGCGGCTTGACCGG.CT.GGCGGCCTCGGGGGCGCCGGTGATTCTGGCGGTGGGC.ATGCCCGCCGTGCCCCCGCCCGAGACCGGGCACCGGCCGACGCAGCGGCGCATCGATCTGCTC  >  NC_014034/1286646‑1286846

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG