Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,303,931 +CA 100% intergenic (+118/+39) urtE → / ← RCAP_rcc01231 urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,303,9311.→C100.0% 152.5 75intergenic (+118/+39)urtE/RCAP_rcc01231urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase
Reads supporting (aligned to +/- strand):  new base (51/24):  ref base (0/0):  total (51/24)
*NC_0140341,303,9312.→A100.0% 154.3 75intergenic (+118/+39)urtE/RCAP_rcc01231urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase
Reads supporting (aligned to +/- strand):  new base (51/24):  ref base (0/0):  total (51/24)

TTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....GCCAAGA..TGGGGG..GC.CCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAGCCCGCCCGC  >  NC_014034/1303857‑1303994
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ttGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGgggcgg                                              >  2:3852948/1‑101 (MQ=14)
 tGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGgggcggg                                             >  1:3730453/1‑101 (MQ=14)
 tGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGgggcggg                                             >  2:289724/1‑101 (MQ=14)
    ggCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCgggccg                                          >  2:1897536/1‑101 (MQ=14)
    ggCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCgggccg                                          <  2:181274/101‑1 (MQ=14)
     gCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCgggccgc                                         >  1:1758879/1‑101 (MQ=14)
      cAAGATAACCCCCACCCTTTCCCCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCgggccgca                                        <  2:3598580/101‑1 (MQ=2)
      cAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGCGAATTGGCCGAAGGCCAAGAGGGGGGGG..GCGCCCCCTTTTCGGGGCgggccgcc                                        >  1:1236477/1‑100 (MQ=12)
      cAAAATAAACCCCATCTTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCgggccgca                                        <  2:1618899/101‑1 (MQ=9)
       aaGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcag                                       >  2:902827/1‑101 (MQ=14)
        aGATAACCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg                                      <  2:1022336/101‑1 (MQ=9)
        aGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg                                      >  2:720527/1‑101 (MQ=14)
         gATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcaggg                                     >  1:1561472/1‑101 (MQ=14)
          aTAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggc                                    >  2:4096517/1‑101 (MQ=14)
          aTAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGCGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCgggccccagggc                                    >  1:729885/1‑101 (MQ=9)
           tAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggcc                                   >  2:337291/1‑101 (MQ=14)
           tAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggcc                                   >  1:3121861/1‑101 (MQ=14)
            aaaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccg                                  >  1:277369/1‑101 (MQ=14)
            aaaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccg                                  >  2:1183999/1‑101 (MQ=14)
            aaaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccg                                  >  2:2755943/1‑101 (MQ=14)
            aaaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCgcgccgcgggtata                                  >  1:4148002/1‑97 (MQ=2)
            aaaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccg                                  >  1:2515029/1‑101 (MQ=9)
               ccccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAc                               >  1:495094/1‑101 (MQ=14)
               ccccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAc                               >  1:343098/1‑101 (MQ=14)
                 ccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAccc                             >  1:1041489/1‑101 (MQ=14)
                  cATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCCCCCt                            >  2:1950147/1‑101 (MQ=9)
                  cATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCt                            >  1:1875246/1‑101 (MQ=14)
                   aTCCTTTCCCCTTGCCGCAAATGTCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTg                           <  1:3391972/101‑1 (MQ=9)
                   aTCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTg                           <  2:873801/101‑1 (MQ=14)
                     ccTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTgcc                         >  2:955140/1‑99 (MQ=9)
                     ccTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTc                         <  2:1782451/101‑1 (MQ=14)
                      cTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCa                        >  2:2574388/1‑101 (MQ=14)
                       tttCACCTTGCCGCAAATATCCCGGGGGGGGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAg                       >  2:569413/1‑101 (MQ=9)
                        ttCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgc                      >  2:2953048/1‑101 (MQ=14)
                        ttCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgc                      >  1:2336722/1‑101 (MQ=14)
                         tccccTGGCCGCAATTACCCGGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgcg                     <  2:2937855/98‑1 (MQ=9)
                         tCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgcg                     <  2:1052266/101‑1 (MQ=14)
                         tCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgcg                     >  2:3761273/1‑101 (MQ=14)
                          cACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgcgc                    >  2:1374795/1‑101 (MQ=17)
                           cccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCa                   <  1:2571444/100‑1 (MQ=9)
                           aCCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCa                   >  1:2482617/1‑101 (MQ=17)
                           aCCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCa                   >  1:57595/1‑101 (MQ=17)
                             cTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATc                 <  1:3414684/101‑1 (MQ=17)
                              ttGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATcc                >  2:2489452/1‑101 (MQ=17)
                              ttGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGACGCAGGGCCGGCCCCCGTCAGAGCATcc                >  2:2440748/1‑101 (MQ=2)
                               tGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATccc               <  2:230960/101‑1 (MQ=17)
                                gcagCAAATCTCCGGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATcccc              <  1:1475190/98‑1 (MQ=12)
                                gCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATcccc              <  2:3332587/101‑1 (MQ=17)
                                gCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATcccc              >  2:2414289/1‑101 (MQ=17)
                                  cGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCaa            <  2:1622509/101‑1 (MQ=17)
                                  cGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCaa            <  1:703689/101‑1 (MQ=17)
                                  cGCAAATACCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCaa            <  2:971688/101‑1 (MQ=12)
                                   gCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAg           >  2:2601793/1‑101 (MQ=17)
                                   gCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAg           <  1:1930832/101‑1 (MQ=17)
                                   gCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAg           >  1:2614464/1‑101 (MQ=17)
                                   gCAAATATCCCGGGGGGGGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGAAGGGCCGCACCCTGTCAGCGCACccccgcg           >  1:4057937/1‑98 (MQ=9)
                                    cAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgc          >  1:2849983/1‑101 (MQ=17)
                                    cAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgc          >  2:629049/1‑101 (MQ=17)
                                     aaaTATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcc         >  2:1635754/1‑101 (MQ=17)
                                     aaaTATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcc         >  2:1561851/1‑101 (MQ=17)
                                     aaaTATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcc         >  1:2051878/1‑101 (MQ=17)
                                      aaTATCCGGGGGGGTGAAGTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgccc        <  2:4119840/101‑1 (MQ=12)
                                      aaTATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgccc        <  1:26126/101‑1 (MQ=17)
                                      aaTATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgccc        >  2:1065187/1‑101 (MQ=17)
                                      aaTATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgccc        >  2:1159689/1‑101 (MQ=17)
                                      aaTATCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgccc        <  2:1695344/101‑1 (MQ=17)
                                       atatCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccg       >  2:3302172/1‑101 (MQ=17)
                                        tatCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccgc      >  2:282599/1‑101 (MQ=18)
                                        tatCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccgc      <  2:438978/101‑1 (MQ=18)
                                         ccccgggggggTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccgcc     <  1:2944731/96‑1 (MQ=12)
                                         atCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccgcc     >  2:614280/1‑101 (MQ=18)
                                         atCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccgcc     >  2:3571587/1‑101 (MQ=18)
                                         atCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccgcc     >  1:508911/1‑101 (MQ=18)
                                         atCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccgcc     >  2:1217831/1‑101 (MQ=18)
                                          tCCCGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccgccc    >  2:777840/1‑101 (MQ=18)
                                           cccGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccgccc  >  1:2686425/1‑100 (MQ=12)
                                           cccGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAGcccgcccg   >  1:1811778/1‑101 (MQ=18)
                                            ccGGGGGGTGAATTGGCCGAAGGCCAAGA..GGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAGCccgcccgc  <  1:3236409/101‑1 (MQ=18)
                                                                                 ||                                                                
TTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....GCCAAGA..TGGGGG..GC.CCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAGCCCGCCCGC  >  NC_014034/1303857‑1303994

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG