Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,550 +C 8.5% intergenic (+362/‑569) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5501.→C 8.5% 76.3 ‑0.2 ‑0.3306intergenic (+362/‑569)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (13/13):  ref base (134/146):  total (147/159)
Fisher's exact test strand distribution p-value = 8.41e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.88e-01

Only 100 of 322 total aligned reads displayed.

GGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGG  >  NC_014034/1395450‑1395650
                                                                                                     |                                                                                                     
 ggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.t                                                                                                      <  2:2075301/101‑1 (MQ=255)
    gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                                   <  2:3829519/101‑1 (MQ=255)
     tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAg                                                                                                  <  2:2219896/101‑1 (MQ=255)
        aTCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCg                                                                                               >  2:3402862/1‑101 (MQ=255)
           gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.cc                                                                                           >  2:3085897/1‑101 (MQ=255)
           atctcATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.cc                                                                                           <  2:912609/97‑1 (MQ=255)
             aaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTg                                                                                         <  1:2257267/101‑1 (MQ=255)
                aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCtc                                                                                      <  2:3556099/101‑1 (MQ=255)
                      gcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCt                                                                                <  2:1581909/101‑1 (MQ=255)
                             cGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCcg                                                                         >  1:3543589/1‑101 (MQ=255)
                              ggTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCcgc                                                                        >  1:2643189/1‑101 (MQ=255)
                               gTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCcccg                                                                       <  1:2416821/101‑1 (MQ=255)
                                  gACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCgggg                                                                    <  2:3638415/101‑1 (MQ=255)
                                   aCAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCggggg                                                                   <  2:814997/101‑1 (MQ=255)
                                    cAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGc                                                                  >  1:1794387/1‑101 (MQ=255)
                                    cAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGc                                                                  >  2:2403158/1‑101 (MQ=255)
                                    cAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGc                                                                  >  2:1083280/1‑101 (MQ=255)
                                      ggCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGt                                                                <  1:3403107/101‑1 (MQ=255)
                                        gccctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTgg                                                              <  1:599133/91‑1 (MQ=0)
                                        agcggctgcggtCCCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTgg                                                              <  2:574877/91‑1 (MQ=0)
                                        aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTgg                                                              >  2:1505818/11‑101 (MQ=2)
                                        aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTgg                                                              >  1:1035369/11‑101 (MQ=2)
                                          ggTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                            <  1:1837026/101‑1 (MQ=255)
                                          ggTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGCCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCggggggggggca                                                            >  2:2793921/1‑94 (MQ=255)
                                          cctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                            <  2:404372/93‑1 (MQ=2)
                                           gccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATc                                                           <  1:1592516/94‑1 (MQ=2)
                                           gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                           <  2:549361/101‑1 (MQ=255)
                                            tAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                          >  2:508899/1‑101 (MQ=255)
                                             cggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                         <  1:3729463/96‑1 (MQ=2)
                                             cgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                         >  1:1951001/6‑101 (MQ=2)
                                             aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                         >  1:1096869/1‑101 (MQ=255)
                                              cgttgcccccccccccGGGGGGGGGGGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                        <  1:3937285/91‑1 (MQ=37)
                                               gtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                       >  2:2618523/4‑101 (MQ=2)
                                               cacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                       <  2:26960/98‑1 (MQ=2)
                                                tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                      >  1:3003947/3‑101 (MQ=2)
                                                 tgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                     <  2:178224/101‑1 (MQ=255)
                                                 ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                     <  2:961627/100‑1 (MQ=2)
                                                 ggtCGCCCCCCCCGCGGGGGCGTGGATCGAAACAAAAACCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                     <  1:1378143/100‑1 (MQ=2)
                                                 cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                                     <  1:444287/100‑2 (MQ=2)
                                                  gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactg                                                    >  1:418309/1‑99 (MQ=2)
                                                  gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacta                                                    <  2:3126514/101‑3 (MQ=2)
                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactat                                                   <  2:58969/101‑4 (MQ=2)
                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                                  <  1:329804/101‑1 (MQ=255)
                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccg                                                  >  2:2358172/1‑98 (MQ=2)
                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccg                                                  >  1:543445/1‑98 (MQ=2)
                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                                  >  1:3801984/1‑97 (MQ=2)
                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                                <  2:4126783/101‑1 (MQ=255)
                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                                >  1:3155118/1‑101 (MQ=255)
                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtg                                                >  2:1986429/1‑95 (MQ=2)
                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCCCGGGGGCGGGGGTCGAAaccgcgtg                                                >  1:764575/1‑96 (MQ=0)
                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgcg                                                >  2:3965954/1‑95 (MQ=2)
                                                       tCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                               >  2:664447/1‑101 (MQ=255)
                                                       tCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                               >  1:2974060/1‑101 (MQ=255)
                                                       tCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAacaccgcgg                                               >  2:3193246/1‑94 (MQ=2)
                                                       tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                                               >  1:2133854/1‑94 (MQ=2)
                                                       tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                                               <  2:1035278/101‑8 (MQ=2)
                                                       tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                                               <  1:1225729/101‑8 (MQ=2)
                                                       tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                                               >  1:3319607/1‑94 (MQ=2)
                                                       cccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                                               <  1:3625092/100‑8 (MQ=2)
                                                        ccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGc                                              <  2:2478634/101‑1 (MQ=255)
                                                        ccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGc                                              >  2:2131950/1‑101 (MQ=255)
                                                        ccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                                              >  2:3931076/1‑94 (MQ=2)
                                                        ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                              >  1:896318/1‑93 (MQ=2)
                                                        ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                              >  2:2425070/1‑93 (MQ=2)
                                                         cccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggc                                             <  1:277709/101‑10 (MQ=2)
                                                            ccGCGGGGGCGTGGATCGAAACATCTCGGCGCCGTCGCTCG.TCCCGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAt                                          <  1:2483203/101‑1 (MQ=255)
                                                             cgcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAtt                                         <  2:3211449/101‑1 (MQ=255)
                                                              gcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTg                                        <  1:3892067/101‑1 (MQ=255)
                                                                gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTggc                                      >  2:1908889/1‑99 (MQ=255)
                                                                gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTgag                                      >  2:1202895/1‑99 (MQ=255)
                                                                gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTGGCGGATTGTc                                      >  1:965997/1‑101 (MQ=255)
                                                                 ggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCa                                     <  2:1722888/101‑1 (MQ=255)
                                                                  gggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCAc                                    <  1:2726450/101‑1 (MQ=255)
                                                                  gggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCAc                                    <  1:3033485/101‑1 (MQ=255)
                                                                  gggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCAc                                    <  1:3678978/101‑1 (MQ=255)
                                                                  gggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCAc                                    >  2:3496251/1‑101 (MQ=255)
                                                                   ggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACt                                   <  2:886684/101‑1 (MQ=255)
                                                                     cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                                 >  1:1354653/1‑101 (MQ=255)
                                                                     cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                                 <  1:1988416/101‑1 (MQ=255)
                                                                      gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                                <  1:117110/101‑1 (MQ=255)
                                                                      gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                                <  2:2539043/101‑1 (MQ=255)
                                                                       tGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                               <  2:4171000/101‑1 (MQ=255)
                                                                       tGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                               <  1:3086127/101‑1 (MQ=255)
                                                                         gATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgcg                             >  1:4031179/1‑101 (MQ=255)
                                                                           tCGAAGCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCggg                           <  2:388774/101‑1 (MQ=255)
                                                                            cGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCgggg                          >  1:174884/1‑101 (MQ=255)
                                                                               aaCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGa                       <  2:180902/101‑1 (MQ=255)
                                                                                 cATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAg                     >  1:2890949/1‑101 (MQ=255)
                                                                                   tctcGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCg                   >  1:1151060/1‑101 (MQ=255)
                                                                                         tGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTc             <  1:336675/101‑1 (MQ=255)
                                                                                          gCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcc            >  1:3451549/1‑101 (MQ=255)
                                                                                           ccGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTccc           <  1:2081875/101‑1 (MQ=255)
                                                                                             gTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTccccc         >  2:3322584/1‑101 (MQ=255)
                                                                                             gTCGCTCG.ACCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTccccc         <  2:1676736/101‑1 (MQ=255)
                                                                                              tCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTTACTTTCGCGGGGCGAAGCGTCGCTcccccc        >  2:1396484/1‑101 (MQ=255)
                                                                                              tCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccccc        <  2:2983493/101‑1 (MQ=255)
                                                                                              tCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccccc        <  2:1354553/101‑1 (MQ=255)
                                                                                                gCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcgc      >  2:3428914/1‑101 (MQ=255)
                                                                                                  tCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCgg    <  1:2403334/101‑1 (MQ=255)
                                                                                                    g.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCgggg  <  2:422659/101‑1 (MQ=255)
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GGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGG  >  NC_014034/1395450‑1395650

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG