Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,692 +T 6.1% intergenic (+504/‑427) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,6921.→T 6.1% 37.4 ‑0.3 ‑0.0181intergenic (+504/‑427)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/4):  ref base (93/77):  total (100/81)
Fisher's exact test strand distribution p-value = 7.57e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.97e-01

Only 100 of 205 total aligned reads displayed.

TCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1395592‑1395792
                                                                                                         |                                                                                                     
tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGa                                                                                                        <  1:4022781/101‑1 (MQ=255)
tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGa                                                                                                        >  2:3549874/1‑101 (MQ=255)
 cGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.g                                                                                                      <  1:546559/101‑1 (MQ=255)
  gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.Gc                                                                                                     <  2:1936796/101‑1 (MQ=255)
  gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.Gc                                                                                                     >  1:2389132/1‑101 (MQ=255)
  gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.Gc                                                                                                     >  1:2611421/1‑101 (MQ=255)
   aaaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.Gcc                                                                                                    >  1:636931/1‑101 (MQ=255)
   aaaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.Gcc                                                                                                    >  1:3930954/1‑101 (MQ=255)
    aaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCg                                                                                                   <  1:1037329/101‑1 (MQ=255)
    aaCCACCTTGCGGATTGTAACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCg                                                                                                   >  2:524881/1‑101 (MQ=255)
      ccaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.Ca                                                                                                >  2:3412179/1‑101 (MQ=255)
        accTTGCGGATTGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGt                                                                                              <  2:440112/101‑1 (MQ=255)
         ccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTc                                                                                             <  1:1691289/101‑1 (MQ=255)
         ccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTc                                                                                             >  1:479137/1‑101 (MQ=255)
          cTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCg                                                                                            <  2:877191/101‑1 (MQ=255)
            tGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCt                                                                                          >  1:893133/1‑101 (MQ=255)
              cGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcc                                                                                        >  1:1327968/1‑101 (MQ=255)
                gATTGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcccc                                                                                      <  1:664524/101‑1 (MQ=255)
                gATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcccc                                                                                      <  1:1557197/101‑1 (MQ=255)
                gATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcccc                                                                                      <  2:174846/101‑1 (MQ=255)
                 aTTGTCACTTTCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTccccc                                                                                     <  2:3389022/101‑1 (MQ=255)
                  ttGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                                                    <  1:2132095/101‑1 (MQ=255)
                  ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                                                    <  1:4150070/101‑1 (MQ=255)
                   tGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCcg                                                                                   >  2:2614954/1‑101 (MQ=255)
                     tCACTTTCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCcgcg                                                                                 <  2:2943881/101‑1 (MQ=255)
                         tttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCggggg                                                                             >  2:429380/1‑101 (MQ=255)
                          ttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGc                                                                            <  2:3874758/101‑1 (MQ=255)
                              ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG..GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTg                                                                         >  2:3429367/10‑101 (MQ=2)
                              ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG..GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTg                                                                         >  2:3624233/10‑101 (MQ=2)
                              gggggCGAAGCGTCCCCCCCCCCGCGGGGGCGGGGATCGAAACATCTGCATGTGGTCGG.CG..G.GGAGTTGGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTgg                                                                        <  1:3893157/100‑1 (MQ=38)
                               ggggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                       >  1:2549836/1‑101 (MQ=255)
                               gatcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCCTGACCGCAT.CGA.A.GCTGGTCAC.ATCG.CCGTCGCTCCCCCCGCGGGGGCGTgg                                                                        <  2:2197809/91‑1 (MQ=2)
                                 gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG..GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                      <  2:2750288/95‑1 (MQ=2)
                                  gCGAGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                    <  2:3346603/101‑1 (MQ=255)
                                    gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                  >  2:22398/1‑101 (MQ=255)
                                     gtgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCGGCAG.TGTCA.GGTTTTCCA.GCGG.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                  <  1:3235548/99‑1 (MQ=2)
                                     ctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG..GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                  <  1:4104645/99‑1 (MQ=2)
                                     catcGTCGCTCCCCCCGCGGGGACGTGGATCGAATCCTATGAGGGCTGGCGGCCC..G.GTCGTTGAT.CCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                   <  2:3165394/98‑1 (MQ=0)
                                      agggTCGCCCCCCCCGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                <  2:853172/98‑1 (MQ=255)
                                       gCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                               >  1:2687011/1‑101 (MQ=255)
                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgg                                                             >  2:3709900/1‑101 (MQ=255)
                                           cGCCCCCCCCGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTa                                                           <  1:2922167/101‑1 (MQ=255)
                                              tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                                        >  2:1603454/1‑101 (MQ=255)
                                              tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                                        >  2:1172760/1‑101 (MQ=255)
                                                  cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                                                    <  2:3153209/101‑1 (MQ=255)
                                                    cgcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTgg                                                  <  2:2499199/101‑1 (MQ=255)
                                                     ggggggcCGTGAATCGAAACATCTGGTTGTGGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                                                 <  2:1048267/99‑1 (MQ=37)
                                                     gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGGTCTGgc                                                 >  1:594519/1‑101 (MQ=255)
                                                     gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                                                 >  1:1403156/1‑101 (MQ=255)
                                                       gggggCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcgc                                               >  2:3200016/1‑101 (MQ=255)
                                                        ggggCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCa                                              >  2:2084684/1‑101 (MQ=255)
                                                         gggCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCaa                                             >  1:3001028/1‑101 (MQ=255)
                                                           gCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAAc                                           >  1:2737661/1‑101 (MQ=255)
                                                            cGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACg                                          >  1:1783396/1‑101 (MQ=255)
                                                             gTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                                         <  1:3185690/101‑1 (MQ=255)
                                                              tGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                                        >  1:3518232/1‑101 (MQ=255)
                                                              gggATCGAACCATCTGCACGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                                        <  2:1913152/100‑1 (MQ=255)
                                                               gggtCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                                       >  1:2416128/4‑101 (MQ=255)
                                                               ggATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                                       <  1:3069006/101‑1 (MQ=255)
                                                               ggATCGAAACATCTGCAGGTGGTCGG.CG..G.GCAGTGCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                                       <  2:1818926/101‑1 (MQ=255)
                                                                gATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa                                      <  2:3930694/101‑1 (MQ=255)
                                                                gATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa                                      >  2:616699/1‑101 (MQ=255)
                                                                gATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAATGGTATCGGTCTGGCGCAAACGGaag                                      >  2:3092418/1‑100 (MQ=255)
                                                                   cGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc                                   >  1:964882/1‑101 (MQ=255)
                                                                   cGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc                                   >  2:554227/1‑101 (MQ=255)
                                                                    gAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt                                  <  2:4028524/101‑1 (MQ=255)
                                                                    gAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt                                  <  2:2609382/101‑1 (MQ=255)
                                                                    gAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt                                  >  1:2364363/1‑101 (MQ=255)
                                                                      aaCATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgt                                <  1:2043358/101‑1 (MQ=255)
                                                                      aaCATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgt                                <  2:1270110/101‑1 (MQ=255)
                                                                         aTCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgt                             <  2:591493/101‑1 (MQ=255)
                                                                          tCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc                            >  2:1643354/1‑101 (MQ=255)
                                                                           cTGCATGTAGGGGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGtcgtcg                           <  1:2951518/101‑1 (MQ=255)
                                                                             gCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCt                         >  2:1465308/1‑101 (MQ=255)
                                                                              cATGGAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTc                        <  1:4114201/101‑1 (MQ=255)
                                                                               aggTAGTCGG.GG..G.GCAGTGCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc                       <  1:1494179/99‑1 (MQ=255)
                                                                               aTGTAGTCGG.CG..G.GCATTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc                       <  1:3710154/101‑1 (MQ=255)
                                                                               aTGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc                       <  2:2686805/101‑1 (MQ=255)
                                                                                 ttggTCGG.CG..G.CAAGTGGGA.GCGG.CAGTCGCTCCCCCCGCCGGGGCGGGGATCTAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccc                     <  2:698051/98‑1 (MQ=25)
                                                                                  tAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTccccc                    >  2:129530/1‑101 (MQ=255)
                                                                                   aGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccccc                   >  2:4101757/1‑101 (MQ=255)
                                                                                    gTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcg                  >  1:1802356/1‑101 (MQ=255)
                                                                                       gg.cg..g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgg               >  1:2788767/1‑101 (MQ=255)
                                                                                       gg.cg..g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgg               >  2:303445/1‑101 (MQ=255)
                                                                                       gg.cg..g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgg               >  1:3280824/1‑101 (MQ=255)
                                                                                       gg.cg..g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgg               >  1:336526/1‑101 (MQ=255)
                                                                                        g.cg..g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCggg              >  1:3469124/1‑101 (MQ=255)
                                                                                          gg..g.gCGGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgggg             <  1:1172854/100‑1 (MQ=255)
                                                                                           g..g.gCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCggggg            >  2:1402118/1‑101 (MQ=255)
                                                                                                 cAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGt         >  1:2812043/1‑101 (MQ=255)
                                                                                                  aGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTg        <  1:1015688/101‑1 (MQ=255)
                                                                                                  aGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTg        >  1:56121/1‑101 (MQ=255)
                                                                                                  aGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTg        >  1:816544/1‑101 (MQ=255)
                                                                                                   ggtCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTgg       <  2:3451297/99‑1 (MQ=255)
                                                                                                    ttCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGa      >  2:2350300/1‑101 (MQ=255)
                                                                                                      cGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATc    >  1:1820771/1‑101 (MQ=255)
                                                                                                      cGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATc    >  2:1885877/1‑101 (MQ=255)
                                                                                                       gA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCg   <  2:3242497/101‑1 (MQ=255)
                                                                                                       gA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCg   >  1:982636/1‑101 (MQ=255)
                                                                                                        a.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGa  <  1:2959564/101‑1 (MQ=255)
                                                                                                         |                                                                                                     
TCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1395592‑1395792

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG