Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,674 +T 1.4% coding (1556/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,6741.→T 1.4% 4.5 ‑2.1 ‑0.2211coding (1556/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (102/106):  total (105/106)
Fisher's exact test strand distribution p-value = 1.21e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.81e-01

Only 100 of 225 total aligned reads displayed.

CCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCC  >  NC_014034/1397574‑1397774
                                                                                                     |                                                                                                     
cccccGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGGTc                                                                                                        >  2:2520440/1‑101 (MQ=255)
cccccGCGGGGGCGTGGATCGAAACAGCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTc                                                                                                        <  2:1858882/101‑1 (MQ=255)
       gggggCGTGGATCGAAACATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCtt                                                                                                <  2:34448/101‑1 (MQ=255)
       gggggCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCtt                                                                                                <  1:1296825/101‑1 (MQ=255)
       gggggCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCtt                                                                                                <  2:862376/101‑1 (MQ=255)
       gggggCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCtt                                                                                                >  2:649587/1‑101 (MQ=255)
       gggggCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCtt                                                                                                >  1:1540328/1‑101 (MQ=255)
       gggggCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCtt                                                                                                >  2:1179047/1‑101 (MQ=255)
        ggggCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtg                                                                                               <  2:2604202/101‑1 (MQ=255)
        ggggCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACTACCGAGTTC.CGGCCTtg                                                                                               >  1:3840510/1‑101 (MQ=255)
        ggggCGGGGATCGAAACATCCGGGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtg                                                                                               <  2:1062358/101‑1 (MQ=255)
         gggCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtgt                                                                                              <  2:1700134/101‑1 (MQ=255)
         gggCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtgt                                                                                              >  2:2647055/1‑101 (MQ=255)
            cGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCCCCCCCCGCGCGGGCGTGGATCCGAACTACAGACGTC.CGGTCTtgttct                                                                                           >  2:4129009/1‑101 (MQ=255)
            cGTGGAGCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtgttct                                                                                           <  2:1251531/101‑1 (MQ=255)
              tGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACTACAGAGGTC.CGGTCTtgttatgt                                                                                         >  1:3177998/1‑101 (MQ=255)
               ggATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtgttctgtt                                                                                        <  2:2907721/101‑1 (MQ=255)
               ggATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtgttctgtt                                                                                        >  1:4115295/1‑101 (MQ=255)
               ggATCGAAACAGCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtgttctgtt                                                                                        <  2:1274690/101‑1 (MQ=255)
                 aTCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTc.c                                                                                     <  2:3279927/101‑1 (MQ=255)
                  tCGAAACAGCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.Cg                                                                                    <  2:615205/101‑1 (MQ=255)
                     aaaCATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTa                                                                                 <  2:3834100/101‑1 (MQ=255)
                        cATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACa                                                                              <  1:315289/101‑1 (MQ=255)
                        cATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACa                                                                              <  2:36641/101‑1 (MQ=255)
                        cATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGGTC.CGGTCTTGTTCTGTTC.CGGTAACa                                                                              >  2:92315/1‑101 (MQ=255)
                           ccGGGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGt                                                                           <  2:2281373/101‑1 (MQ=255)
                            cgcgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTc                                                                          >  2:3126602/1‑101 (MQ=255)
                              cgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAAAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGc                                                                        >  1:1426199/1‑101 (MQ=255)
                               ggATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCt                                                                       >  1:233870/1‑101 (MQ=255)
                                gATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTc                                                                      >  1:2895940/1‑101 (MQ=255)
                                gATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTc                                                                      <  1:1360530/101‑1 (MQ=255)
                                gATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTc                                                                      >  2:1148260/1‑101 (MQ=255)
                                 aTCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTcc                                                                     >  1:2601557/1‑101 (MQ=255)
                                   cGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTT.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTcccc                                                                   >  1:2970047/1‑101 (MQ=255)
                                   cGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTcccc                                                                   >  1:3005901/1‑101 (MQ=255)
                                     gCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTcccccc                                                                 <  1:2749360/101‑1 (MQ=255)
                                     gCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTcccccc                                                                 <  1:1602878/101‑1 (MQ=255)
                                      cGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCcg                                                                <  2:3188804/101‑1 (MQ=255)
                                       gTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCcgc                                                               >  1:1163460/1‑101 (MQ=255)
                                        tgccccgccccgcCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCcgcg                                                              <  1:1692404/98‑1 (MQ=255)
                                         gACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCgg                                                             >  1:3881260/1‑101 (MQ=255)
                                           cccgccccgcCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCCCCCCCCGCgggg                                                           <  2:308183/101‑2 (MQ=255)
                                             cgccccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGgggc                                                         <  1:1097532/101‑1 (MQ=255)
                                               ccccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGt                                                       <  1:3924951/101‑1 (MQ=255)
                                                cccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTg                                                      >  2:296648/1‑101 (MQ=255)
                                                  cgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGa                                                    <  2:233832/101‑1 (MQ=255)
                                                   gcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                   <  2:719843/101‑1 (MQ=255)
                                                   gcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                   >  2:4118111/1‑101 (MQ=255)
                                                       aTCGGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGGCGCTCCCCCCGCGGGGGCCTGGATCGaa                                               >  2:3257074/1‑101 (MQ=38)
                                                        tCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                              <  2:1724494/101‑1 (MQ=255)
                                                           gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.TGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACtt                                           <  2:2159440/101‑1 (MQ=255)
                                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGt                                         >  1:3594174/1‑101 (MQ=255)
                                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTc                                        >  1:433688/1‑101 (MQ=255)
                                                                tCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTg                                      <  1:4198810/101‑1 (MQ=255)
                                                                cccccccGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTg                                      <  1:1217039/100‑1 (MQ=255)
                                                                  cccccGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGAc                                    <  2:739997/101‑1 (MQ=255)
                                                                   ccccGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGAcg                                   >  2:2734980/1‑101 (MQ=255)
                                                                   ccccGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGAcg                                   <  1:3485799/101‑1 (MQ=255)
                                                                    cccGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGAcgc                                  <  1:853490/101‑1 (MQ=255)
                                                                     ccGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGAcgcg                                 <  2:1476225/101‑1 (MQ=255)
                                                                     ccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGGTTGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCtatggcg                                 >  1:4035443/1‑95 (MQ=0)
                                                                      cgcgGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACgcgc                                >  2:4098398/1‑101 (MQ=255)
                                                                        cgGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtc                              >  2:2881780/1‑101 (MQ=255)
                                                                         gggggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                             <  1:2488645/101‑1 (MQ=255)
                                                                          ggggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcat                            >  2:2395733/1‑101 (MQ=255)
                                                                          ggggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcat                            >  2:3729209/1‑101 (MQ=255)
                                                                           gggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatc                           >  2:2056144/1‑101 (MQ=255)
                                                                           gggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatc                           >  1:2190360/1‑101 (MQ=255)
                                                                            ggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatca                          <  2:1040106/101‑1 (MQ=255)
                                                                             gCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAg                         <  2:2443937/101‑1 (MQ=255)
                                                                              cGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGc                        >  2:1965393/1‑101 (MQ=255)
                                                                                tGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCAc                      >  2:3695727/1‑101 (MQ=255)
                                                                                 ggATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACTCGCTCATCAGCACg                     >  1:2092540/1‑101 (MQ=255)
                                                                                 ggATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg                     <  1:1007483/101‑1 (MQ=255)
                                                                                 ggATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg                     >  1:563041/1‑101 (MQ=255)
                                                                                    tCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCGCGCGGGGGCGTGGATCGAAACTTGTCTGAAGCGCTCATCAGCACGtga                  >  2:3505344/1‑99 (MQ=255)
                                                                                     cGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCaa                 >  1:602668/1‑101 (MQ=255)
                                                                                       aaaCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa               <  2:1027310/101‑1 (MQ=255)
                                                                                        aaCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGAt              >  1:2128417/1‑101 (MQ=255)
                                                                                        aaCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGAt              <  1:2647261/101‑1 (MQ=255)
                                                                                         aCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGAtt             >  2:1601116/1‑101 (MQ=255)
                                                                                         aCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGAtt             >  1:2432250/1‑101 (MQ=255)
                                                                                         aCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGAtt             >  1:4234640/1‑101 (MQ=255)
                                                                                          cTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTc            <  2:2432074/101‑1 (MQ=255)
                                                                                          cTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTc            <  1:738615/101‑1 (MQ=255)
                                                                                          cTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTc            <  2:1207242/101‑1 (MQ=255)
                                                                                           tACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCa           >  2:1375027/1‑101 (MQ=255)
                                                                                           tACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCa           <  2:3881001/101‑1 (MQ=255)
                                                                                           tACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCa           >  2:3620267/1‑101 (MQ=255)
                                                                                           tACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGTCTGACGCGCTCCTCAGCACGTCAAGATTCa           >  1:3673331/1‑101 (MQ=255)
                                                                                           tACAGAGTTC.CGGCCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGACCGAAACTTGCCTGGCGCTCCCATCAGCACGCCAAGATTCa           >  2:473696/1‑101 (MQ=25)
                                                                                            aCAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAg          >  2:1526034/1‑101 (MQ=255)
                                                                                              agagTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTc        <  1:2520624/101‑1 (MQ=255)
                                                                                              agagTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTc        <  1:1312816/101‑1 (MQ=255)
                                                                                                agTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGc      >  2:553592/1‑101 (MQ=255)
                                                                                                 gTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCt     >  1:697384/1‑101 (MQ=255)
                                                                                                 gTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCt     >  1:1884347/1‑101 (MQ=255)
                                                                                                  ttC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTc    >  1:3326708/1‑101 (MQ=255)
                                                                                                  ttC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGGCGCTc    >  2:4140164/1‑101 (MQ=255)
                                                                                                   tC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcc   <  1:2040668/101‑1 (MQ=255)
                                                                                                     |                                                                                                     
CCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCC  >  NC_014034/1397574‑1397774

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG