Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,606,281 +T 1.9% coding (13/1083 nt) mraY → phospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,606,2811.→T 1.9% 8.5 ‑3.5 ‑0.1212coding (13/1083 nt)mraYphospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (85/123):  total (89/123)
Fisher's exact test strand distribution p-value = 2.98e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.01e-01

Only 100 of 244 total aligned reads displayed.

CGAAAGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAT.CACCTTCCGCGCCGGTGGGGCCTTCTTCACCGCGCTGATCTTCGGCTTCCT  >  NC_014034/1606181‑1606381
                                                                                                     |                                                                                                     
cggaaGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGc                                                                                                        <  2:2631466/98‑1 (MQ=255)
 gAAAGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.t                                                                                                      >  1:2643023/1‑101 (MQ=255)
 gAAAGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.t                                                                                                      <  1:1054062/101‑1 (MQ=255)
 gAAAGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.t                                                                                                      >  2:1950589/1‑101 (MQ=255)
 gAAAGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.t                                                                                                      >  1:157174/1‑101 (MQ=255)
  aaaGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAAAAAAGCTTTACTGGC.tt                                                                                                     >  1:2802233/1‑101 (MQ=255)
   aaGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTg                                                                                                    <  1:933406/101‑1 (MQ=255)
   aaGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGACCGGCGCCGCAGAGGCCTTGAAGGGAAAAGAGTAATGCTTTACTGGC.ttt                                                                                                    >  2:2417336/1‑100 (MQ=255)
        tCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.ttttttac                                                                                               >  1:3175816/1‑95 (MQ=255)
        tCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAAc                                                                                               <  1:2708420/101‑1 (MQ=255)
         cGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.ttttttact                                                                                              >  2:1255294/1‑94 (MQ=255)
          gAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.tttnnttcct                                                                                             >  1:1734570/1‑93 (MQ=255)
           aaGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCtt                                                                                            <  1:2619772/101‑1 (MQ=255)
           aaGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCtt                                                                                            <  2:1768287/101‑1 (MQ=255)
            aGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.ttttttatcctt                                                                                           >  2:827429/1‑91 (MQ=255)
            aGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCttt                                                                                           <  2:596419/101‑1 (MQ=255)
              gTTTCGCTCGTTGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCg                                                                                         <  2:157136/101‑1 (MQ=255)
               tttCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCgg                                                                                        <  1:3458946/101‑1 (MQ=255)
                 tCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGAc                                                                                      <  1:3339403/101‑1 (MQ=255)
                 tCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGAc                                                                                      <  1:327366/101‑1 (MQ=255)
                 tCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGAc                                                                                      <  1:1004926/101‑1 (MQ=255)
                   gCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACgg                                                                                    <  1:1282476/101‑1 (MQ=255)
                    cTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACggg                                                                                   <  1:2056004/101‑1 (MQ=255)
                    cTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACggg                                                                                   <  1:61750/101‑1 (MQ=255)
                      cGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTTTTACCTTTTCGACggggg                                                                                 >  1:527288/1‑101 (MQ=255)
                       ttGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGc                                                                                <  1:3257643/100‑1 (MQ=255)
                       gTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTTTTACCTTTCTGACgggggg                                                                                >  1:1047425/1‑100 (MQ=255)
                       gTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGc                                                                                <  1:195586/101‑1 (MQ=255)
                       gTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGc                                                                                <  2:3746713/101‑1 (MQ=255)
                        tGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTTGGACCTTTTGGGCgcggggg                                                                               >  2:3700209/1‑99 (MQ=255)
                        cgatctaCGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGAGAACCTTTCGGACGGGGGCg                                                                               <  1:3892360/95‑1 (MQ=255)
                          gTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGAt                                                                             <  1:1905117/101‑1 (MQ=255)
                           ttGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTTGTACCTTTTTGGCggggggggtt                                                                            >  1:2737596/1‑96 (MQ=255)
                           ttGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGAtt                                                                            <  1:248480/101‑1 (MQ=255)
                           ttGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGAtt                                                                            <  2:2555878/101‑1 (MQ=255)
                            tGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGAttt                                                                           <  1:700809/101‑1 (MQ=255)
                            tGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGAttt                                                                           <  1:4170012/101‑1 (MQ=255)
                             gACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTTGATACTTTCCGTCGGGGGCgggttt                                                                          >  2:2720994/1‑96 (MQ=255)
                             gACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGTGAACCTTTCGGACGGGGGCGATttc                                                                          <  2:691869/101‑1 (MQ=255)
                              gcgcgCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGATttct                                                                         <  1:2087658/100‑1 (MQ=255)
                              aCGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTTGTGCCTTTTTGACgggggggggttgt                                                                         >  1:1182538/1‑93 (MQ=255)
                              aCGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGATttct                                                                         <  2:1157330/101‑1 (MQ=255)
                              aCGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGAGAACCTTTCGGACGGGGGCgggatcg                                                                         >  2:3892101/1‑95 (MQ=255)
                                gcgcTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTTGTACCCTTTTGACgggggggggtttttt                                                                       >  1:2330795/1‑91 (MQ=255)
                                      aGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTTGTACCCTTCCTATGGGGGGGGTTTTTTTAAccac                                                                 >  1:2843327/1‑99 (MQ=255)
                                      aGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACctc                                                                 <  2:3024041/101‑1 (MQ=255)
                                        aaaaCTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCtt                                                               <  2:3931164/101‑1 (MQ=255)
                                          aaCTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTcc                                                             <  1:3143478/101‑1 (MQ=255)
                                           aCTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTTGGACCCTTCTGGCGGGGGGGGTTTTTTTAAACCCtttcc                                                            >  1:1414721/1‑98 (MQ=255)
                                               ggTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTAc                                                        <  2:1871511/101‑1 (MQ=255)
                                                gTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTTGAACCTTTTTGGCGGGGGGGGGTTTTTTAACCTCTTCCCCTACa                                                       >  1:2216971/1‑101 (MQ=255)
                                                 tCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAt                                                      <  1:3919738/101‑1 (MQ=255)
                                                 tCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAt                                                      <  1:1454040/101‑1 (MQ=255)
                                                  cAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTTGAACCTTTCTGACGGGGGGGGTTTCTTTTACCCCTTccccctcatc                                                     >  2:3650759/1‑93 (MQ=255)
                                                   aGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAT.Ca                                                   <  1:823533/101‑1 (MQ=255)
                                                      ccGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGGTATTTCTTCAACCTCTTCCGCTACAT.CACCt                                                <  1:3769494/101‑1 (MQ=255)
                                                       cGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTTGGACCTTTTGGGCGGGGGGGGGTTTTTTTACCTCTTCCCCTTCCT.CACCtt                                               >  1:1442969/1‑101 (MQ=255)
                                                         gcgcGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAT.CACCTTcc                                             <  1:3711610/101‑1 (MQ=255)
                                                             ggCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTTCCGAACCTTCGGGAGGGGGGGGGTTT.TTCAACCTCTTCCCCTACAT.CCcccttcccgc                                         >  1:2569979/1‑93 (MQ=255)
                                                                agagGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAT.CACCTTCCGCGCCgg                                      <  1:3845134/101‑1 (MQ=255)
                                                                agagGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAT.CACCTTCCGCGCCgg                                      <  2:2159223/101‑1 (MQ=255)
                                                                 gagGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTCGAACCCTTCTGACGGGGGGGGGTTTTTTTACCTCTTCCCCCACAT.CACCTTCCCCGCCgcg                                     >  1:2281838/1‑99 (MQ=255)
                                                                 gagGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAT.CACCTTCCGCGCCGGt                                     <  1:3067191/101‑1 (MQ=255)
                                                                 gagGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAT.CACCTTCCGCGCCGGt                                     <  1:3409089/101‑1 (MQ=255)
                                                                  agGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTTGAACCTTTTGGGCGGGGGGGGTTTTTTTTAACTCTTCCGCTGCAC.CACCTTTCCCGCCGGTg                                    >  1:4115787/1‑101 (MQ=255)
                                                                  agGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAT.CACCTTCCGCGCCGGTg                                    <  2:3595399/101‑1 (MQ=255)
                                                                   ggCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAT.CACCTTCCGCGCCGGTgg                                   <  2:1926850/101‑1 (MQ=255)
                                                                   ggCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAT.CACCTTCCGCGCCGGTgg                                   <  2:2397240/101‑1 (MQ=255)
                                                                    gCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAT.CACCTTCCGCGCCGGTggg                                  <  1:1255390/101‑1 (MQ=255)
                                                                     ccTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAT.CACCTTCCGCGCCGGTgggg                                 <  1:946393/101‑1 (MQ=255)
                                                                      cTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTCGACCCTTTTCTGCGGGGGGGGTTTCTTCTACCTCTTCCGCTCCTT.CCCCTCTCGCCCCgtctgggc                                >  2:1987867/1‑94 (MQ=255)
                                                                       ttCAAGGGAAAAGAATAATGCTTTACTGGC.TTTGGAACCTTTCGGACGGGGGGGGTTTTTTCAACCTCTTCCGCTACAT.CACCTTTCCCGCCCGGgggggc                               >  1:836616/1‑99 (MQ=255)
                                                                        tCAAGGGAAAAGAATAATGCTTTACTGGC.TTTCGGACCTTTCCGGCGGGGGGGGGTTTTTCAAACTCTTCCCCTTCATCCCCCTTCCCCCCCGGGGGGGcc                               >  2:765644/1‑101 (MQ=255)
                                                                        tCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAT.CACCTTCCGCGCCGGTGGGGCct                              <  2:1853555/101‑1 (MQ=255)
                                                                         cAAGGGAAAAGAATAATGCTTTACTGGC.TTTTGGAACTTTCGGGCGGGGGGGGTTTTTTTAACCTCTTCCCCTACAT.CCCCCTCCCCGCCCGGGGGGGctt                             >  2:2926805/1‑101 (MQ=255)
                                                                         cAAGGGAAAAGAATAATGCTTTACTGGC.TTTCGAACCTTTTGGACGGGGGGGGTTTTTTTAAACTCTTCCCCTACAA.CACCCTCCCCCCCGGGGGGGGctt                             >  2:3386398/1‑101 (MQ=255)
                                                                         cAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAT.CACCTTCCGCGCCGGTGGGGCctt                             <  1:3645882/101‑1 (MQ=255)
                                                                          aaGGGAAAAGAATAATGCTTTACTGGC.TTTCGAACCCTTCGGACGGGGGGGGTTTCTTTCACCCCTTCCCCTACCT.CACCCTCCCCCCCGGTGGGGGcttc                            >  1:3676512/1‑101 (MQ=255)
                                                                           aGGGAAAAGAATAATGCTTTACTGGC.TTTCGGACCCTTTGGGCGGGGGGGGGTTTTTTAACCCCTTCCGCTACAT.CACCTTCCCCGCCCGGGGGGcgcttt                           >  2:1614238/1‑96 (MQ=255)
                                                                           aGGGAAAAGAATAATGCTTTACTGGC.TTTCGAACCTTTTGGGCGGGGGCGGGTTCTTCAACCTCTTCCCCTACCT.CACCCTTCCCGCCGCTGGGGGcttct                           >  2:286047/1‑101 (MQ=255)
                                                                            gggAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAT.CACCTTCCGCGCCGGTGGGGCcttctt                          <  1:4037541/101‑1 (MQ=255)
                                                                             ggAAAAGAATAATGCTTTACTGGC.TTTTGAACCTTTCGGGCGGGGGGGGTTTCTTTAAACTCTTCCCCTACAT.CACCCTCCCCGCCCGGGGGGGcctcttt                         >  2:3784636/1‑100 (MQ=255)
                                                                              gAAAAGAATAATGCTTTACTGGC.TTTCGAACCTTTTGGGCGGGGGGGGGTTCTTCAAACTCTTCCCCTACAT.CCCCTTCCGCCCCCGGGGGGGcttctttc                        >  2:3239820/1‑99 (MQ=255)
                                                                               aaaaGAATAATGCTTTACTGGC.TTTCGAACCTTTCGGGCGGGGGGGGTTTTTTTCACCTCTTCCCCTACAT.CACCTTCCGCCCCGGGGGGGGCTTCTTTAc                       >  1:3447463/1‑101 (MQ=255)
                                                                                aaaGAATAATGCTTTACTGGC.TTTCGAACCTTTCGGGCGGGGGGGGTTTTTTCAACCCCTTTCCCTACAT.CACCTTCCCCCCCCGGGGGGGCTTTTTCAcc                      >  1:1143861/1‑101 (MQ=255)
                                                                                   gAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGGGGTTTTTTCAACCTCTTCCGCTACAT.CACCTTCCTCGCCGGCGGGGGCTTCTTCACcgcc                   >  2:3247755/1‑100 (MQ=255)
                                                                                      taatGCTTTACTGGC.TTTCGAACCCTTTGGACGGGGGGGGTTTCTTCAACCTCTTCCGCTACAT.CACCTTCCGCGCCGGTGGGGGCTTCTTTAGCgcgccg                >  2:335680/1‑99 (MQ=255)
                                                                                       aatGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGGGGTTTTTTCAACCTCTTTCGCTACAT.CACCTTCCGCGCCCGCGGGGCCTTCTTCACCCCGCTgc               >  2:3285845/1‑100 (MQ=255)
                                                                                        atGCTTTACTGGC.TTTCGAACCTTTCTGGCGGGGGGGATTTTTTCAAACTCTTTCCCTACAT.CACCTTCCGCGCCCGTGGGGGCCTCTTCACCGCGCTGAt              >  2:1353516/1‑101 (MQ=255)
                                                                                           cTTTACTGGC.TTGCGAACCTTTCGGACGGGGGGGATTTCTTCAACCTCTTCCGCTACAT.CACCTTCCGCGCCCGTGGGGGCTTCTTCACCGCGCCGATCtt           >  2:1395766/1‑101 (MQ=255)
                                                                                            tttACTGGC.TTGCGAACCTTTTGGGCGGGGGGGGTTTCTTCAACCTCTTCCGCTACAT.CACCTTCCGCGCCGGTGGGGCCTTCTTCTCCGCGCCGATCTTc          >  2:1104220/1‑101 (MQ=255)
                                                                                            tttACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAT.CACCTTCCGCGCCGGTGGGGCCTTCTTCACCGCGCTGATCTTc          <  2:2815155/101‑1 (MQ=255)
                                                                                             ttACTGGC.TTGCGAACCTTTCGGGCGGGGGGGAGTTTTTCCAACTCTTTCGCTACAT.CACCCTCCGCGCCGGGGGGGGCCTCTTCCCCGCGCTGATCTTCg         >  2:792881/1‑101 (MQ=255)
                                                                                             ttACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAT.CACCTTCCGCGCCGGTGGGGCCTTCTTCACCGCGCTGATCTTCg         <  1:2927024/101‑1 (MQ=255)
                                                                                             ttACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAT.CACCTTCCGCGCCGGTGGGGCCTTCTTCACCGCGCTGATCTTCg         <  2:2216812/101‑1 (MQ=255)
                                                                                              tACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAT.CACCTTCCGCGCCGGTGGGGCCTTCTTCACCGCGCTGATCTTCgg        <  2:3439165/101‑1 (MQ=255)
                                                                                                 tGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAT.CACCTTCCGCGCCGGTGGGGCCTTCTTCACCGCGCTGATCTTCGGCtt     >  2:43262/1‑101 (MQ=255)
                                                                                                 tGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAT.CACCTTCCGCGCCGGTGGGGCCTTCTTCACCGCGCTGATCTTCGGCtt     >  2:15470/1‑101 (MQ=255)
                                                                                                   gC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAT.CACCTTCCGCGCCGGTGGGGCCTTCTTCACCGCGCTGATCTTCGGCTTcc   >  1:4014269/1‑101 (MQ=255)
                                                                                                    c.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAT.CACCTTCCGCGCCGGTGGGGCCTTCTTCACCGCGCTGATCTTCGGCTTCCt  >  2:4002809/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
CGAAAGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGCGAACCTTTCGGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAT.CACCTTCCGCGCCGGTGGGGCCTTCTTCACCGCGCTGATCTTCGGCTTCCT  >  NC_014034/1606181‑1606381

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG