Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_014034 | 1,640,005 | +GGCA | 100% | intergenic (+85/‑60) | mccB → / → mccA | methylcrotonoyl‑CoA carboxylase subunit beta/methylcrotonoyl‑CoA carboxylase subunit alpha |
Read alignment evidence... | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | position | change | freq | score | reads | annotation | genes | product | ||
* | NC_014034 | 1,640,005 | 1 | .→G | 100.0% | 12.1 | 68 | intergenic (+85/‑60) | mccB/mccA | methylcrotonoyl‑CoA carboxylase subunit beta/methylcrotonoyl‑CoA carboxylase subunit alpha |
Reads supporting (aligned to +/- strand): new base (60/1): ref base (1/5): total (62/6) | ||||||||||
Fisher's exact test strand distribution p-value = 3.68e-06 | ||||||||||
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.58e-03 | ||||||||||
* | NC_014034 | 1,640,005 | 2 | .→G | 100.0% | 12.2 | 67 | intergenic (+85/‑60) | mccB/mccA | methylcrotonoyl‑CoA carboxylase subunit beta/methylcrotonoyl‑CoA carboxylase subunit alpha |
Reads supporting (aligned to +/- strand): new base (59/1): ref base (1/5): total (61/6) | ||||||||||
Fisher's exact test strand distribution p-value = 3.97e-06 | ||||||||||
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.44e-02 | ||||||||||
* | NC_014034 | 1,640,005 | 3 | .→C | 100.0% | 15.3 | 67 | intergenic (+85/‑60) | mccB/mccA | methylcrotonoyl‑CoA carboxylase subunit beta/methylcrotonoyl‑CoA carboxylase subunit alpha |
Reads supporting (aligned to +/- strand): new base (59/1): ref base (2/5): total (61/6) | ||||||||||
Fisher's exact test strand distribution p-value = 1.27e-05 | ||||||||||
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.57e-02 | ||||||||||
* | NC_014034 | 1,640,005 | 4 | .→A | 100.0% | 15.5 | 68 | intergenic (+85/‑60) | mccB/mccA | methylcrotonoyl‑CoA carboxylase subunit beta/methylcrotonoyl‑CoA carboxylase subunit alpha |
Reads supporting (aligned to +/- strand): new base (60/0): ref base (2/5): total (62/6) | ||||||||||
Fisher's exact test strand distribution p-value = 2.17e-06 | ||||||||||
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.37e-03 |
Only 100 of 104 total aligned reads displayed.
GATCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGG.....G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCGC > NC_014034/1639905‑1640087
||||
taTCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..gggg < 2:2749422/100‑1 (MQ=255)
aTCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGGTAATT.TGGGG.GAGATGAAA.ACCATCCCTTT.CATC.CTGCCCCAAATAT.ACCCGGGGGCC..C..ggcg.....g > 2:723589/1‑101 (MQ=255)
aTCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGAAATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..gggg.....g > 1:2749641/1‑101 (MQ=255)
tCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGGTATTT.TGGGG.AAGATGAAA.CCCATCCCCTT.CTCC.TTTCCCCCAAAAT.CCCCGGGGGCC..C..gggg.....gg > 1:2008801/1‑100 (MQ=255)
tCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGGTATTT.TGGGG.AAAATGAAA.ACCATCCCCTT.CATC.TTTCCCCAAATAT.CCCCGGGGGCC..C..cggg.....gg > 1:675195/1‑100 (MQ=37)
tCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..gggg.....gg < 1:2351668/101‑2 (MQ=255)
tCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..gggg.....gg < 1:2427775/101‑2 (MQ=255)
cTTCCGGGTGGGACTTGCGATGCCTCCGGGGGGGATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..gggg.....ggc < 1:3595389/101‑3 (MQ=25)
cTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGAGATTT.TGGGG.AAAATGAAA.CCCCTCCCTTT.CATC.TTTCCCCAAATAT.CCCCGGGGGCC..C..cggg.....ggg > 1:1156766/1‑99 (MQ=37)
ttCCGGATTTGCCTTTCGATGCCGCGGGGGGGGATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..gggg.....ggca < 2:3579021/101‑4 (MQ=21)
ttCCGGATGTGACTTTCGATGCCTCCGGCGGGGGAATTT.TGGGG.AAGATGAAA.ACCCTCCCTTT.TATC.TTTCCCCCAATAT.CCCCGGGGGCC..C..gggg.....gggg > 1:1854123/1‑98 (MQ=25)
ttCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..gggg.....ggca < 1:1567082/101‑4 (MQ=255)
ttCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..gggg.....ggca < 2:4235210/101‑4 (MQ=255)
ttCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..gggg.....ggca < 1:1261497/101‑4 (MQ=255)
ttCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTT.GAGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..gggg.....ggca < 1:3165721/101‑4 (MQ=255)
tCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..gggg.....ggcag < 1:1953103/101‑5 (MQ=255)
cGGATGTGACTTTCGATGCCTCCGGCGGGGATATTT.GGGGC.AAGAGGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..gggg.....ggcagcg < 1:3642807/101‑7 (MQ=255)
ggATGTGACTTTCGATGCCTCCGGCGGGGGTATTT.TGGGG.AAGATGAAA.ACCATCCCTTT.TTTC.TTTCCCCCAATATACCCGGGGGGCC..G..gggg.....ggggggg > 2:3588770/1‑95 (MQ=19)
ggATGTGACTTTCGATGCCTCCGGCGGGGGTATTT.TGGGC.AAGAAGGAA.CCCACCCCCTT.CATC.CTGCCCCAAATAT.CCCGGGGGGCC..C..gggg.....ggcggcgc > 1:525002/1‑94 (MQ=25)
gATGTGACTTTCGATGCCTCCGGCGGGGGTATTT.TGGGG.AAGATGAAA.ACCATCCCTTT.CATC.TTTCCCCCAAAAT.CCCCGGGGGCC..C..gggg.....ggcagggcc > 1:2640565/1‑93 (MQ=25)
gATGTGACTTTCGATGCCTCCGGCGGGGAGATTT.TGGGG.AAGATTAAA.CCCATCCCCCT.CGTC.TTTCCCCCAACAT.ACCTGGGCGCC..C..gggg.....ggcggcgtc > 2:317627/1‑93 (MQ=255)
tgtgACTTTCGATGCCTCCGGCGGGGGTATTT.TGGGG.AAGAAGAAA.ACCATCCCCTT.TATCTTTCCCCCAAAAAT.CCCGGGGGGCC..C..gggg.....ggagcggccc > 2:3572823/1‑92 (MQ=255)
tgtgACTTTCGATGCCTCCGGCGGGGGTATTT.GGGGG.CAGATAGAA.CACATCCCTTT.TCTC.TTGCCCCCAACATCCCCGGGGGGCC..G..GGGG.....G.....CG.....g..cgcccc > 2:2288896/1‑100 (MQ=255)
aTGCCTCCGGCGGGGATATTT.GGGGC.AAGATGAAA.CCCCTCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGG.....GGGGN.CG.....C..CCCCCGCCACTTcc > 2:509320/1‑101 (MQ=21)
cTCGGGCGGGTTTTTTG.GGGCC.AAGAT.AAA.CCCACCCCTTT.TCTT.CTGCCCCAAATTC.CCCGGGGGGGCGGG..GGGG.....G.....CGGCC..C..CCCCCGCCACTTCCGCCa < 2:1802052/101‑1 (MQ=255)
ccggggggggGTATTTGGGGGA.AAGAAAAAC.CCCCCCCCTTT.CACC.CTGCCCCAAATTC.CCCGGGGGGCC..G..GGGG.....GGC...CGCCC..C..CCCCCGCCACTTCCGcc < 2:1296645/95‑1 (MQ=255)
ggcggGGATATTT.TGGGC.AAGATGAAA.ACCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..CCGGGGGCA...G.....CG.....C..CCCCCCCCCCTTCCGCCACggg > 2:1750600/1‑101 (MQ=18)
cggGGATATTT.GGGGC.AAGATGAAA.CCGATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGc > 2:1271544/1‑101 (MQ=21)
ggggATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGNA.G.....CN.....C..CCCCCGCCACTTCCGCCACGGGGCa > 1:4222481/1‑101 (MQ=21)
ggggATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCa > 1:4156993/1‑101 (MQ=25)
ggggATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCa > 1:1193964/1‑101 (MQ=25)
gggaatttt.GGGGA.AAAAATAAACCCCACCCTTTT.CATC.TTCCCCAAAATA..CCCGGGGGGCC..C..GGGG.....GGC...CGCCC..C..CCCCCGCCACTTCCGCCACGGGGCa < 2:2504854/95‑1 (MQ=2)
ggATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAgc > 1:2318810/1‑101 (MQ=21)
gATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCAccgatctgaa > 2:2711613/1‑92 (MQ=18)
gATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAgcg > 2:1760374/1‑101 (MQ=25)
atatTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCAGCCACGGGGCAgcgc > 2:818114/1‑101 (MQ=21)
atatTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGG.....GGC...CG.....CGACCCCCGCCACTTCCGCCACGGGGCAgcgc > 2:1038688/1‑101 (MQ=21)
tgggggC.AAGA.AAAA.CCCCCCCCTTT.CCCC.CTCCCCCAAAATC.CCCGGGGGGCC..C..GGGG.....GGC...CGGCC..C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCg < 2:3862991/99‑1 (MQ=255)
tgggggC.AAAATG.AA.CCCCCCCCCTT.CCTC.TTTCCCCAAAATC.CCCGGGGGGCC..C..GGGGGGCCAG.....CC.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCg < 1:3482028/99‑1 (MQ=255)
t.GGGGCAAAGATAAAC.CCCCCCCCTTT.CTTC.TTCCCCAAAATAT.CCGGGGGGCCC..G..GGGG.....GCC...CGCC...C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCg < 2:2856948/101‑1 (MQ=9)
t.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCgg > 1:729171/1‑101 (MQ=25)
t.GGGGC.AAAGAGAAA.CCCCCCCCTTTCCCTC.TTCCCCCAAATA..CCCGGGGGGCC..C..GGGGGGGCAG.....CC.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCg < 2:1223608/101‑1 (MQ=255)
tgggcgaaaGATAAAC.CCCACCCCTTT.CATC.TCGCCCAAAATA..CCCGGGGGGCC..CGGGGGG.....G.....CGGCC..C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCg < 2:1207293/94‑1 (MQ=12)
gggggA.AAGATAAAC.CCCACCCTTTT.CATC.TGCCCCAAAAGAC.CGGGGGGGGCG..G..GGGG.....GGCCC.CC.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCgg < 2:3308683/100‑1 (MQ=255)
ggggC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGc > 1:979752/1‑101 (MQ=25)
ggggC.AAAAAAAAA.CCCCCCCCTTT.TCTC.CTGCCCCAAAATC.CCCGGGGGGCC..CGGGGGG.....G.....CGCCC..C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCgg < 1:2500847/101‑1 (MQ=255)
ggaaaaagAAC.CCCCTCCCTTT.TCTT.TTCCCCCAAAATT.CCGGGGGGGCC..C..GGGG.....GGGCCCCC.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCg < 1:3154015/94‑1 (MQ=255)
c.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGtt > 1:3714772/1‑101 (MQ=21)
c.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGtt > 1:3197070/1‑101 (MQ=25)
aaGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGTCAGCGCCCCGGCCGTTg > 2:3801608/1‑101 (MQ=21)
aaGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTg > 2:3148872/1‑101 (MQ=25)
aaGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTg > 2:476050/1‑101 (MQ=25)
aaGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTg > 1:3512785/1‑101 (MQ=25)
aGATGAAA.CCCATCCCTTT.CATC.TTTCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGa > 1:696071/1‑101 (MQ=21)
gATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGAc > 2:2111578/1‑101 (MQ=21)
aTGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACg > 1:2055857/1‑101 (MQ=21)
tGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGa > 1:229503/1‑101 (MQ=25)
tGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGa > 1:1327950/1‑101 (MQ=25)
agcaa.CCCCTCCCTTT.CCCT.TTCCCCCAAAAGA.CCCGGGGGGCCGGG..GGGG.....G.....CGCCC..C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACg < 1:2520481/98‑1 (MQ=255)
gAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGaa > 2:105336/1‑101 (MQ=25)
gAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGaa > 1:2494886/1‑101 (MQ=25)
aa.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAgg > 1:96620/1‑101 (MQ=25)
a.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGga > 2:3396684/1‑101 (MQ=25)
cccATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGgag > 1:4188376/1‑101 (MQ=25)
cATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGAc > 2:1430903/1‑101 (MQ=25)
cccctttt.CCTC.TTCCCCCAAATCC.CCGGGGGGGCC..G..GGGG.....GCCCC.CC.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGAcc < 1:1254920/96‑1 (MQ=255)
aTCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGAcc > 1:1408632/1‑101 (MQ=25)
tCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACcg > 1:1624093/1‑101 (MQ=25)
tCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACcg > 1:2684234/1‑101 (MQ=25)
ccccTTT.CCCC.CTCCCCAAAATCT.CCCGGGGGGCC..C..GGGG.....GGGCCCCC.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGAcc < 1:218398/100‑1 (MQ=255)
ccTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACcgcg > 2:4164487/1‑101 (MQ=25)
cTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGa > 1:2484647/1‑101 (MQ=25)
ttt.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGAt > 1:2824194/1‑101 (MQ=25)
tt.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATg > 2:3563178/1‑101 (MQ=25)
tt.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATg > 1:1091080/1‑101 (MQ=25)
t.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGt > 1:15707/1‑101 (MQ=25)
t.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGt > 1:1838639/1‑101 (MQ=25)
t.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGt > 1:2241139/1‑101 (MQ=25)
cATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATggt > 1:980072/1‑99 (MQ=25)
cATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGtt > 1:204490/1‑101 (MQ=25)
cATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGtt > 2:1963307/1‑101 (MQ=25)
cATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGtt > 2:1814031/1‑101 (MQ=25)
tccccccccccAAAATT.CCGGGGGGGCC..C..GGGG.....GGCC..CGCC...C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGt < 1:2660119/94‑1 (MQ=255)
aTC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGttt > 1:3487541/1‑101 (MQ=25)
tC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTc > 2:4220085/1‑101 (MQ=25)
tC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTc > 2:3738016/1‑101 (MQ=25)
ctcccccccAAATCC.CCGGGGGGGCC..C..GGGG.....G.....GGCCCCCC..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGttt < 1:2248123/96‑1 (MQ=255)
c.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCTCCGGCCGTTGACGAAGGAGACCGCGATGTTTCa > 1:3643864/1‑101 (MQ=25)
c.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGAGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCa > 2:1398167/1‑101 (MQ=25)
tGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGCAGACCGCGATGTTTCaaa > 1:4015837/1‑101 (MQ=25)
ccccAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAg > 2:1861649/1‑101 (MQ=25)
aaaTAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATcc > 2:3708778/1‑101 (MQ=25)
aaaTAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATcc > 1:939176/1‑101 (MQ=25)
aaTAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCt > 1:2303192/1‑101 (MQ=25)
atat.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTg > 2:47642/1‑101 (MQ=37)
atat.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTg > 1:2178667/1‑101 (MQ=37)
tat.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGTCGAAGGAGACCGCGATGTTTCAAAAGATCCTGa > 1:3677459/1‑101 (MQ=25)
at.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGAt > 1:4056427/1‑101 (MQ=37)
t.CCCGGGGGGCC..C..GGGG.....GGCCC.CG.....C..CCCCCCCCCCTTCCGCCGCGGGGCAACGCCCCCGCCGGTGTCGAAGCGGAGGCCGACGGTTCAAAAGATCCTGATc > 2:3688719/1‑101 (MQ=255)
nccGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCNGATcn > 1:963020/2‑100 (MQ=37)
||||
GATCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGG.....G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCGC > NC_014034/1639905‑1640087
Base quality scores: 
ATCG
< 3 ≤
ATCG
< 15 ≤
ATCG
< 25 ≤
ATCG
< 33 ≤
ATCG
< 40 ≤
ATCG
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