Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,696,177 +C 91.0% intergenic (‑105/+120) RCAP_rcc01564 ← / ← mmsA isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,696,1771.→C91.0% 49.6 ‑1.5 ‑0.9201intergenic (‑105/+120)RCAP_rcc01564/mmsAisobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  new base (72/109):  ref base (10/8):  total (84/117)
Fisher's exact test strand distribution p-value = 2.17e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.38e-01

Only 100 of 219 total aligned reads displayed.

GCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTC.GG.CAATTCCA.CCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAG  >  NC_014034/1696075‑1696274
                                                                                                        |                                                                                                  
gcTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.gg                                                                                                     >  2:861080/1‑101 (MQ=255)
gcTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.gg                                                                                                     >  2:384790/1‑101 (MQ=255)
gcTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCCAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.gg                                                                                                     <  1:3136135/101‑1 (MQ=255)
  tcctccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GG.cc                                                                                                  >  2:2000837/1‑100 (MQ=255)
    ctccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GG.ccaa                                                                                                >  1:3413884/1‑98 (MQ=255)
     tccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GG.ccaat                                                                                               <  2:759626/101‑5 (MQ=255)
      cccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GG.ccaatt                                                                                              <  2:3258439/101‑6 (MQ=255)
      cccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GG.ccaatt                                                                                              >  1:2616819/1‑96 (MQ=255)
      cccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGCCCTTTC.GG.ccaatt                                                                                              >  1:570062/1‑96 (MQ=255)
       ccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTc                                                                                              <  2:2210227/101‑1 (MQ=255)
        cAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTca                                                                                             <  2:2036576/101‑2 (MQ=255)
        cAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTca                                                                                             >  2:3929199/1‑100 (MQ=255)
         aaaGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTcac                                                                                            <  1:733058/101‑3 (MQ=255)
         aaaGGGCCT.AAATGAACGTCTGTTTAATTTNGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GG.CCAATTCA.cc                                                                                         <  1:1458385/101‑1 (MQ=255)
          aaGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GG.CCAATTCA.cc                                                                                         <  2:3590879/101‑1 (MQ=255)
          aaGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GG.CCAATTCA.cc                                                                                         <  1:3696061/101‑1 (MQ=255)
           aGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GG.CCAATTCA.ccc                                                                                        >  2:926325/1‑101 (MQ=255)
           aGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GG.CCAATTCA.ccc                                                                                        >  1:249543/1‑101 (MQ=255)
            gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GG.CCAATTCA.cccc                                                                                       >  2:2670416/1‑100 (MQ=255)
            gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GG.CCAATTCA.cccc                                                                                       <  1:2095960/101‑2 (MQ=37)
            gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GG.CCAATTCA.cccc                                                                                       >  2:1747692/1‑100 (MQ=255)
             tGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GG.CCAATTCA.ccccg                                                                                      >  2:4059117/1‑99 (MQ=255)
                cTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGa                                                                                    >  2:641394/1‑101 (MQ=255)
                cTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGa                                                                                    >  2:1376230/1‑101 (MQ=255)
                  gAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGatg                                                                                  <  1:3067310/101‑3 (MQ=25)
                   aaTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGatgt                                                                                 <  2:782878/101‑4 (MQ=255)
                   aaTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGatgt                                                                                 <  2:299402/101‑4 (MQ=255)
                   aaTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGatgt                                                                                 >  1:2305139/1‑98 (MQ=255)
                    aTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGatgtt                                                                                <  2:1726741/101‑5 (MQ=255)
                     ttGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGatgttt                                                                               >  2:4083874/1‑96 (MQ=255)
                     ttGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TCCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGatgttt                                                                               <  1:1738427/101‑6 (MQ=255)
                        aaCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCCATTCAC.CCCGGGATGTTTggg                                                                            >  1:154355/1‑101 (MQ=255)
                         aCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.AGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGc                                                                           >  1:2482356/1‑101 (MQ=255)
                          cGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGcc                                                                          >  2:2594102/1‑101 (MQ=25)
                          cGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGcc                                                                          <  1:4059378/101‑1 (MQ=255)
                           gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCa                                                                         >  1:1133717/1‑101 (MQ=255)
                            tCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCaa                                                                        >  2:2330463/1‑101 (MQ=255)
                              tGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCaagg                                                                      >  1:1108984/1‑99 (MQ=255)
                               gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.TCCGGGATGTTTGGGCCaaggt                                                                     <  1:2405590/101‑4 (MQ=255)
                               gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCaaggt                                                                     >  1:1686309/1‑98 (MQ=255)
                               gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCaaggt                                                                     <  2:295682/101‑4 (MQ=255)
                               gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCaaggt                                                                     <  1:4232443/101‑4 (MQ=255)
                               gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAAGgg                                                                     >  1:3860893/1‑101 (MQ=255)
                                tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGACAATTCAC.CCCGGGAGGTTTGGGCCAAGgga                                                                    >  2:1762370/1‑100 (MQ=255)
                                 ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtga                                                                   <  1:4081391/101‑6 (MQ=16)
                                 ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.CGCCCCTCCTGGCCTTTC.GGCCAATTAAC.CCCGGGATTTTTGGGCCAAGggga                                                                   >  1:356887/1‑100 (MQ=255)
                                 ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC..CCTGGCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGCTGTTTGGGCCaacgtga                                                                   >  1:1723910/1‑96 (MQ=255)
                                  tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtgaa                                                                  <  1:214508/101‑7 (MQ=255)
                                  tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtgaa                                                                  >  1:3470800/1‑95 (MQ=255)
                                  tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtgaa                                                                  >  2:2143540/1‑95 (MQ=255)
                                   aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtgaaa                                                                 <  1:4101421/101‑8 (MQ=255)
                                   aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtgaaa                                                                 <  1:4008949/101‑8 (MQ=255)
                                   aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtgaaa                                                                 <  1:3794677/101‑8 (MQ=255)
                                     tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAaca                                                             >  1:2382112/1‑100 (MQ=255)
                                      ttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacac                                                            >  1:3243274/1‑99 (MQ=12)
                                        cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacct                                                          >  2:2170299/1‑97 (MQ=255)
                                        cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacct                                                          <  2:3330481/101‑5 (MQ=255)
                                         gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                         <  2:3443688/101‑6 (MQ=255)
                                          aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                        <  2:3495203/101‑7 (MQ=12)
                                          aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                        <  1:1201293/101‑7 (MQ=12)
                                          aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                        >  1:193247/1‑95 (MQ=255)
                                          aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                        <  2:1964853/101‑7 (MQ=12)
                                          aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                        <  1:457876/101‑7 (MQ=12)
                                           aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                       >  2:3054120/1‑94 (MQ=255)
                                             ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                     <  1:3498769/101‑10 (MQ=9)
                                             ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                     <  1:863606/101‑10 (MQ=9)
                                              cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                    <  1:3728606/101‑11 (MQ=255)
                                              cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                    <  2:3216506/101‑11 (MQ=255)
                                                 gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                               >  2:3743203/1‑101 (MQ=255)
                                                 gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                               <  1:955162/101‑1 (MQ=255)
                                                  cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt                                              <  2:2532239/101‑1 (MQ=255)
                                                   aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                             <  2:1108890/101‑1 (MQ=9)
                                                   aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                             <  1:786670/101‑1 (MQ=9)
                                                   aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                             <  2:53398/101‑1 (MQ=9)
                                                      aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCCCCCGGCCTTTCTGC.CAATTCAC.CCCCGGATGTTTGGGCCCA..GGTGGAA.CA.CCTTGCTTTTTCTTGgc                                          >  2:809034/1‑101 (MQ=255)
                                                        ttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc                                        <  1:1570650/101‑1 (MQ=255)
                                                        ttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc                                        >  1:2593942/1‑101 (MQ=9)
                                                          ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                      >  1:2793385/1‑101 (MQ=255)
                                                           tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                                     <  2:4000554/101‑1 (MQ=255)
                                                            gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                    <  1:3811798/101‑1 (MQ=9)
                                                                ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                                >  2:165009/1‑101 (MQ=255)
                                                                 gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                               >  1:447266/1‑101 (MQ=9)
                                                                 gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                               <  1:413763/101‑1 (MQ=255)
                                                                  aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                              <  1:1315218/101‑1 (MQ=9)
                                                                    gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                            <  2:3104716/101‑1 (MQ=12)
                                                                    gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                            <  2:549700/101‑1 (MQ=255)
                                                                    gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                            <  2:2359735/101‑1 (MQ=255)
                                                                          gaggggcgctGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTTGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTTGGGCAAATACGCCTCTGGcc                        >  1:3758137/10‑101 (MQ=255)
                                                                           aggggcgctGCCCCTCCTGGCCTTTC.GGCCAATTCTC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTTCTTTTTCTTGGCGCAAATACGCCTCTGGccc                       <  2:1706647/93‑1 (MQ=255)
                                                                            ggggcgctGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg                      <  2:4164183/94‑1 (MQ=255)
                                                                                 gctGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAggg                 >  2:3703779/3‑101 (MQ=16)
                                                                                  ctGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc                >  2:2870644/2‑101 (MQ=16)
                                                                                   tGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa               >  2:1248457/1‑101 (MQ=255)
                                                                                    gCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa              <  1:641471/101‑1 (MQ=255)
                                                                                     ccccTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg             <  2:1412046/101‑1 (MQ=19)
                                                                                     ccccTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg             >  1:1000892/1‑101 (MQ=19)
                                                                                        cTCCTGGCCTTTC.GGCCAATTCCC.CCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcg          <  1:2978002/101‑1 (MQ=21)
                                                                                             ggCCTTTC.GG.CCAATCCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg     <  2:2414690/101‑1 (MQ=19)
                                                                                              gCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGa    >  1:2248752/1‑101 (MQ=255)
                                                                                                cTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg  <  1:3209124/101‑1 (MQ=19)
                                                                                                        |                                                                                                  
GCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTC.GG.CAATTCCA.CCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAG  >  NC_014034/1696075‑1696274

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG