Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,729,654 +C 100% intergenic (+28/+125) RCAP_rcc01597 → / ← tpiA AsnC/Lrp family transcriptional regulator/triose‑phosphate isomerase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,729,6541.→C100.0% 423.4 118intergenic (+28/+125)RCAP_rcc01597/tpiAAsnC/Lrp family transcriptional regulator/triose‑phosphate isomerase
Reads supporting (aligned to +/- strand):  new base (61/55):  ref base (0/2):  total (61/57)

Only 100 of 141 total aligned reads displayed.

GGTGAAGATCTACAACGTCACAGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTG.CCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTGATTTCATCTTGCTCCAAATATCCCGG  >  NC_014034/1729554‑1729748
                                                                                                     |                                                                                              
ggTGAAGATCTACAACGTCACAGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTg                                                                                                 >  1:3514947/1‑101 (MQ=255)
ggTGAAGATCTACAACGTCACAGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTg                                                                                                 <  1:1710718/101‑1 (MQ=255)
  tGAAGATCTACAACGTCACAGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTG.cc                                                                                              <  2:3270789/101‑1 (MQ=255)
   gAAGATCTACAACGTCACAGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTG.ccc                                                                                             <  2:2861200/101‑1 (MQ=255)
    aaGATCTACAACGTCACAGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTG.cccc                                                                                            >  2:2731424/1‑101 (MQ=255)
    aaGATCTACAACGTCACAGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTG.cccc                                                                                            <  2:105307/101‑1 (MQ=255)
       aTCTACAACGTCACAGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTG.cccccct                                                                                         <  2:753489/101‑3 (MQ=255)
        tCTACAACGTCACAGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTG.cccccctc                                                                                        <  1:893695/101‑4 (MQ=255)
        tCTACAACGTCACAGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTG.cccccctc                                                                                        <  1:2731630/101‑4 (MQ=255)
         cTACAACGTCACAGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTG.cccccctct                                                                                       >  1:799196/1‑97 (MQ=255)
         cTACAACGTCACAGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTG.cccccctct                                                                                       <  1:2666844/101‑5 (MQ=255)
         cTACAACGTCACAGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTG.cccccctct                                                                                       <  2:2380628/101‑5 (MQ=255)
          tACAACGTCACAGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTG.cccccctctg                                                                                      <  2:611828/101‑6 (MQ=255)
           aCAACGTCACAGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCCGCCCCCCTCTgg                                                                                      >  2:701004/1‑101 (MQ=255)
              aCGTCACAGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCt                                                                                   <  2:2525355/101‑1 (MQ=255)
               cGTCACAGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTc                                                                                  >  2:325082/1‑101 (MQ=255)
                gTCACAGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTcg                                                                                 <  1:2639983/101‑1 (MQ=255)
                 tCACAGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTcgc                                                                                <  2:2135949/101‑1 (MQ=255)
                 tCACAGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTcgc                                                                                <  2:2456627/101‑1 (MQ=255)
                  cacaGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTcgcg                                                                               >  1:342670/1‑101 (MQ=255)
                   acaGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCgg                                                                              >  1:3990202/1‑101 (MQ=255)
                   acaGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCgg                                                                              <  1:1919437/101‑1 (MQ=255)
                     aGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGcc                                                                            <  2:2560841/101‑1 (MQ=255)
                     aGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGcc                                                                            <  2:4088583/101‑1 (MQ=255)
                      gCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCa                                                                           >  2:2065346/1‑101 (MQ=255)
                      gCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCa                                                                           <  2:2662966/101‑1 (MQ=255)
                      gCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCa                                                                           <  1:2490557/101‑1 (MQ=255)
                        aCTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCAtt                                                                         <  2:1672602/101‑1 (MQ=255)
                         cTTGTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTc                                                                        <  1:2189056/101‑1 (MQ=255)
                         cTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTc                                                                        <  1:2604859/101‑1 (MQ=255)
                         cTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTc                                                                        >  2:4041781/1‑101 (MQ=255)
                           tCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCAc                                                                      >  1:2100266/1‑101 (MQ=255)
                           tCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGGGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCAc                                                                      >  1:2827963/1‑101 (MQ=255)
                                gCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCAcccccc                                                                 <  1:2582830/101‑1 (MQ=255)
                                gCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCAcccccc                                                                 <  2:2787247/101‑1 (MQ=255)
                                 cATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCg                                                                <  1:1172422/101‑1 (MQ=255)
                                   tGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCggg                                                              >  2:277059/1‑101 (MQ=255)
                                    ggAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCCTTCACCCCCCggca                                                             >  2:1475419/1‑99 (MQ=255)
                                     gAATAGAGCAAGTCGACGACGATTCTGGCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCTCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGat                                                            <  2:2218316/101‑1 (MQ=37)
                                        gagaTCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATAtt                                                         <  2:1598545/101‑1 (MQ=255)
                                         gcctCAAGTCGACGGCGGTTCCGCCGCTGTGGCCCGGTTGGGCCGGGCAGGGGGGCACCGCCCCCCTCTGGCCTCGCGACCATTCCCCCCCCGGGATAttt                                                        <  2:1322402/98‑1 (MQ=16)
                                         agaTCAAGTCGACGACGATTCTGCCGGTGTGGCCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCCCCCCCCGGGATAttt                                                        <  1:2145214/101‑1 (MQ=37)
                                         agaTCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATAttt                                                        >  1:909108/1‑101 (MQ=255)
                                          gaTCCAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGGGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTg                                                       <  1:224606/101‑1 (MQ=37)
                                            tCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGa                                                     >  2:3083550/1‑101 (MQ=255)
                                             cAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAg                                                    >  1:2523740/1‑101 (MQ=255)
                                              aaGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGc                                                   >  1:3183628/1‑101 (MQ=255)
                                                gTCGACGACGATTCTGGCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCaa                                                 <  1:725405/101‑1 (MQ=255)
                                                 tCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAg                                                >  2:1946151/1‑101 (MQ=255)
                                                   gacgacgaTTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGAt                                              >  2:2436397/1‑101 (MQ=255)
                                                     cgacgaTTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGa                                            >  2:2774130/1‑101 (MQ=255)
                                                     cgacgaTTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGa                                            <  1:3416263/101‑1 (MQ=255)
                                                      gacgaTTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGaa                                           >  1:1640508/1‑101 (MQ=255)
                                                        cgaTTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGaaaa                                         >  1:3860694/1‑101 (MQ=255)
                                                         gaTTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGaaaaa                                        >  2:1626901/1‑101 (MQ=255)
                                                         gaTTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGaaaaa                                        <  2:3723272/101‑1 (MQ=255)
                                                          aTTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAaac                                       >  1:1834674/1‑101 (MQ=255)
                                                          aTTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAaac                                       >  1:2627135/1‑101 (MQ=255)
                                                          aTTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAaac                                       >  1:696506/1‑101 (MQ=255)
                                                          aTTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAaac                                       >  2:2452531/1‑101 (MQ=255)
                                                           ttCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAaaca                                      <  2:2009789/101‑1 (MQ=255)
                                                           ttCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTCGAGCAAGATGAAAaaca                                      >  2:2029211/1‑101 (MQ=255)
                                                            tCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAaaaaa                                     >  2:2242486/1‑101 (MQ=255)
                                                             cTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAaacaac                                    <  1:346971/101‑1 (MQ=255)
                                                             cTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAaacaac                                    <  2:3194676/101‑1 (MQ=255)
                                                              tGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAAcc                                   >  1:1622976/1‑101 (MQ=255)
                                                              tGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAAcc                                   >  2:3352760/1‑101 (MQ=255)
                                                                ccGGTGTGCCCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCCCCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCgg                                 <  2:554751/101‑1 (MQ=37)
                                                                 cGGTGTGACCCGGTCGGGCCGGGCTGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGa                                <  1:371182/101‑1 (MQ=255)
                                                                  ggTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAg                               <  2:3937709/101‑1 (MQ=255)
                                                                  ggTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAg                               >  2:3385444/1‑101 (MQ=255)
                                                                     tcggaccGGTCGGGCCGGCCAGGGGTGCGCGGCCCCCCTCTGGCCTCGCCCACATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTg                            <  1:2530809/96‑1 (MQ=21)
                                                                     gtgGCCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTg                            <  1:1761034/101‑1 (MQ=255)
                                                                      tgACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGTGCAAGATGAAAAACAACCGGAGCTGa                           >  1:3852375/1‑101 (MQ=255)
                                                                      tgACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTGa                           <  1:1920393/101‑1 (MQ=255)
                                                                       gACCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTGAt                          <  2:2456620/101‑1 (MQ=255)
                                                                        aCCCGGTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTGAtt                         >  2:4087509/1‑101 (MQ=255)
                                                                         cccGGGCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTGAttt                        <  1:3850001/101‑1 (MQ=255)
                                                                           cGGTCGGGCCGGGCAGCGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCGCCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTGATTTCa                      <  2:1864939/101‑1 (MQ=37)
                                                                            ggTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTGATTTCAt                     >  2:4217364/1‑101 (MQ=255)
                                                                            ggTCGGGCCGGGCAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTGATTTCAt                     >  1:2230671/1‑101 (MQ=255)
                                                                               cgggccgggcAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGGAAGATGAAAAACAACCGGAGCTGATTTCATCtt                  >  1:2929398/1‑101 (MQ=255)
                                                                                 ggccgggcAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTGATTTCATCTTGc                >  2:2346757/1‑101 (MQ=255)
                                                                                  gaggggaAGGGGGGCGCTGCCCCCCTCTGGGCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTGATTTCATCTTGCt               <  2:2562612/98‑1 (MQ=25)
                                                                                    cgggcAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTGATTTCATCTTGCTcc             <  2:2616577/101‑1 (MQ=255)
                                                                                     gggcAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATTAAAAACAACCGGAGCTGATTTCATCTTGCTCCa            >  2:1799940/1‑101 (MQ=255)
                                                                                     gggcAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTGATTTCATCTTGCTCCa            >  2:34703/1‑101 (MQ=255)
                                                                                     gggcAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTGATTTCATCTTGCTCCa            >  1:1279949/1‑101 (MQ=255)
                                                                                     cggcAGGCGCGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTGATTTCATCTTGCTCCa            <  2:1570002/100‑1 (MQ=25)
                                                                                       gcAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTGATTTCATCTTGCTCCaaa          >  2:741401/1‑101 (MQ=255)
                                                                                        gaGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTGATTTCATCTTGCTCCAAat         <  2:528821/100‑1 (MQ=255)
                                                                                        cAGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGGGCAAGATGAAAAAAAACCGGAGCTGGTTTCAACTTGCTCCAAat         >  1:1073576/1‑101 (MQ=18)
                                                                                         aGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCCCCCTCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTGATTTCATCTTGCTCCAAata        <  1:3126253/101‑1 (MQ=37)
                                                                                         aGGGGGGCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTGATTTCATCTTGCTCCAAata        >  1:1556997/1‑101 (MQ=255)
                                                                                          ggggggCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTGATTTCATCTTGCTCCAAatat       >  1:1117232/1‑101 (MQ=255)
                                                                                          ggggggCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTGATTTCATCTTGCTCCAAatat       <  1:807027/101‑1 (MQ=255)
                                                                                           gggggCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTGATTTCATCTTGCTCCAAATATc      >  1:408390/1‑101 (MQ=255)
                                                                                              ggCGCTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTGATTTCATCTTGCTCCAAATATCCCg   <  1:824375/101‑1 (MQ=255)
                                                                                               gcgcTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTGATTTCATCTTGCTCCAAATATCCCgg  <  1:2208805/101‑1 (MQ=255)
                                                                                               gcgcTGCCCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTGATTTCATCTTGCTCCAAATATCCCgg  <  1:1008048/101‑1 (MQ=255)
                                                                                                     |                                                                                              
GGTGAAGATCTACAACGTCACAGCACTTCTGAGCATGGAAGAGATCAAGTCGACGACGATTCTGCCGGTGTGACCCGGTCGGGCCGGGCAGGGGGGCGCTG.CCCCCTCTGGCCTCGCGGCCATTCACCCCCCGGGATATTTGGAGCAAGATGAAAAACAACCGGAGCTGATTTCATCTTGCTCCAAATATCCCGG  >  NC_014034/1729554‑1729748

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG