Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,732,702	+GG	100%	intergenic (‑126/‑277)	RCAP_rcc01601 ← / → tgt	FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,732,702	1	.→G	100.0%	83.9	34	intergenic (‑126/‑277)	RCAP_rcc01601/tgt	FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (0/33):  ref base (0/0):  total (0/34)
*	NC_014034	1,732,702	2	.→G	100.0%	88.4	34	intergenic (‑126/‑277)	RCAP_rcc01601/tgt	FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (0/34):  ref base (0/0):  total (0/34)

TATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.A.CC..GGGC..GGGCGG..G..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATG  >  NC_014034/1732604‑1732778                                                                                                          ||                                                                                 tatataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGAGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGATTCGCCGCGGC.GGGGCCGCGGCGG.C.Cc..gggc..g                                                                                 >  2:3195183/1‑101 (MQ=255)    ctaGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCc..gggc..gggg                                                                              <  2:1168233/100‑2 (MQ=255)    ataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCc..gggc..gggg                                                                              <  2:1494699/101‑2 (MQ=255)    ataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCc..gggc..gggg                                                                              <  2:2131535/101‑2 (MQ=255)      aGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGGTTCGCCGCGGCGGGGGCCCGGGG.G.C.Cc..gggc..gggggg                                                                            >  1:1168327/1‑101 (MQ=255)       gggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.A.CC..CGGG..GGGGgg..g..g                                                                      >  2:2690075/1‑101 (MQ=255)        ggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCc..gggc..gggggctc                                                                          <  2:4193958/101‑6 (MQ=255)        ggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCc..gggc..gggggctc                                                                          <  1:265525/101‑6 (MQ=255)        ggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCc..gggc..gggggctc                                                                          <  1:3070559/101‑6 (MQ=255)         gTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCc..gggc..gggggctcc                                                                         <  2:1002518/101‑7 (MQ=255)         gTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCc..gggc..gggggctcc                                                                         <  1:1477786/101‑7 (MQ=255)          tATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCc..gggc..gggggctccg                                                                        <  1:3862549/101‑8 (MQ=39)          tATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCc..gggc..gggggctccg                                                                        <  1:3251709/101‑8 (MQ=39)           aTCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCc..gggc..gggggctccgc                                                                       <  2:2286278/101‑9 (MQ=38)           aTCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..CGGC..GGGGgg..g..gggg                                                                   >  1:2256525/1‑98 (MQ=255)            tCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.A.CCCGGGGC..GGGGgg..g..ggcg                                                                   >  2:1792202/1‑98 (MQ=255)             cGGGGGGAGAGGTAAAGGCCGTCGGTGGGGGGCGGAAAGCTGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCc..gggc..gggggctccgccc                                                                     <  2:2116995/101‑11 (MQ=37)             cGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCc..gggc..gggggctccgccc                                                                     <  1:1632546/101‑11 (MQ=38)               gggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGGCGCGGC.C.A.CC..CGGG..GGGGgg..g..ggggggccc                                                              >  1:2766531/1‑93 (MQ=255)                ggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGGCGCGGC.G.A.CC..CGGG..GGGGGG..G..Tccccccccc                                                             >  1:4151882/1‑97 (MQ=255)                   gagaGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.A.CC..CCGG..GGGGGG..G..GGCCCCCCCcccc                                                          >  1:970843/1‑101 (MQ=255)                      agGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCAcccc                                                          <  2:4151620/101‑1 (MQ=25)                       ggTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCAccccc                                                         <  1:3287269/101‑1 (MQ=25)                       ggTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCAccccc                                                         <  2:3159941/101‑1 (MQ=25)                       ggTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCAccccc                                                         <  2:1678316/101‑1 (MQ=25)                       ggTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCAccccc                                                         <  2:1609783/101‑1 (MQ=25)                         tAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCgg                                                       <  1:3278563/101‑1 (MQ=25)                         tAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCgg                                                       <  2:2756478/101‑1 (MQ=25)                         tAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGC..GGGGGG..G..CCGCCCCCCccccccccc                                                     >  1:414817/1‑97 (MQ=255)                          aaaGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGC..GGGGGG..G..GCGCCCCCCcccccccccg                                                    >  2:2528008/1‑96 (MQ=255)                                cGTCGGTGCGGGGCGGACGGCCGGGTGCCAAGCAGGGTTTCGCCGCGGG.GGGGCCGCGGC.GCC.CC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTAttt                                                <  2:1530854/101‑1 (MQ=16)                                 gTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTg                                               <  1:1894797/101‑1 (MQ=21)                                  tCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTgc                                              <  1:1054370/101‑1 (MQ=21)                                  tCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGC..GGGGGG..GCCGCCCCCGCCCCCCCCGCggcctttg                                              >  2:1030967/1‑94 (MQ=255)                                      tGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCa                                          <  2:3485951/101‑1 (MQ=21)                                         ggggCGGAAAGCCGCGTGCCAGGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGTGCCaaga                                       <  2:1265441/101‑1 (MQ=18)                                         ggggCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTCTTTGCGCCaaga                                       <  1:2298605/101‑1 (MQ=21)                                         ggggCAGAAGGCCGCGTGCCCAGAATGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGT..GGGGTGTCC..GCCCCCCCACCTCCGGCTTATTTGCGTCaaga                                       <  2:1080930/101‑1 (MQ=255)                                          gggCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCaagaa                                      <  2:941662/101‑1 (MQ=21)                                           gcgagAAAGCCGCGTGCCAGGCGGGGGTGCGCGGCGGG.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCaagaag                                     <  2:1469560/97‑1 (MQ=9)                                             cGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAgaagaa                                   <  1:3872664/101‑1 (MQ=18)                                              ggAGAGCCGCGTGCCAAGCGGGGTTTCGCCGGGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGaaa                                  <  1:2921000/101‑1 (MQ=17)                                              ggAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGaaa                                  <  2:759145/101‑1 (MQ=18)                                              ggAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGaaa                                  <  2:3776171/101‑1 (MQ=18)                                               gAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAAc                                 <  2:163316/101‑1 (MQ=18)                                               gAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAAc                                 <  1:2126933/101‑1 (MQ=18)                                                    ccGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCg                            <  2:1785524/101‑1 (MQ=18)                                                       cgTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGa                         <  2:3437024/101‑1 (MQ=18)                                                        gTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGAc                        <  2:2892352/101‑1 (MQ=18)                                                         tGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGAcc                       <  1:1954841/101‑1 (MQ=18)                                                           ccAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAg                     <  1:2690281/101‑1 (MQ=18)                                                            cAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAgg                    <  2:2133135/101‑1 (MQ=18)                                                             aaGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGc                   <  2:2256366/101‑1 (MQ=18)                                                              aGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGcc                  <  1:3210292/101‑1 (MQ=18)                                                                  gggTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCTGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGcccccc              <  2:3061714/101‑1 (MQ=18)                                                                    gTTTCGCCGCGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTg            <  1:3254114/101‑1 (MQ=18)                                                                        cgccgcGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGc        <  2:2680733/101‑1 (MQ=18)                                                                          ccgcGGC.GGGGCCGCGGC.GTG.CC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCg      <  1:2523589/101‑1 (MQ=21)                                                                          ccgcGGC.GGGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCg      <  1:428965/101‑1 (MQ=18)                                                                             cggc.ggGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCAt   <  2:2857051/101‑1 (MQ=18)                                                                              ggc.ggGGCCGCGGC.G.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATg  <  1:2528192/101‑1 (MQ=18)                                                                                                          ||                                                                                 TATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGC.G.A.CC..GGGC..GGGCGG..G..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATG  >  NC_014034/1732604‑1732778 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG