| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_014034 | 1,732,866 | +GCG | 100% | intergenic (‑290/‑113) | RCAP_rcc01601 ← / → tgt | FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase |
| Read alignment evidence... | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | change | freq | score | reads | annotation | genes | product | ||
| * | NC_014034 | 1,732,866 | 1 | .→G | 100.0% | 15.7 | 21 | intergenic (‑290/‑113) | RCAP_rcc01601/tgt | FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase |
| Reads supporting (aligned to +/- strand): new base (6/9): ref base (0/6): total (6/15) | ||||||||||
| Fisher's exact test strand distribution p-value = 1.23e-01 | ||||||||||
| Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.60e-03 | ||||||||||
| * | NC_014034 | 1,732,866 | 2 | .→C | 100.0% | 15.7 | 21 | intergenic (‑290/‑113) | RCAP_rcc01601/tgt | FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase |
| Reads supporting (aligned to +/- strand): new base (6/9): ref base (0/6): total (6/15) | ||||||||||
| Fisher's exact test strand distribution p-value = 1.23e-01 | ||||||||||
| Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.72e-04 | ||||||||||
| * | NC_014034 | 1,732,866 | 3 | .→G | 100.0% | 15.7 | 21 | intergenic (‑290/‑113) | RCAP_rcc01601/tgt | FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase |
| Reads supporting (aligned to +/- strand): new base (6/9): ref base (0/6): total (6/15) | ||||||||||
| Fisher's exact test strand distribution p-value = 1.23e-01 | ||||||||||
| Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.72e-04 | ||||||||||
CTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCG.CTTTTCCTAGGC.AAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...CCCCCC...GT...C....CG..CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCGGC > NC_014034/1732766‑1732948
|||
cTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCG.CTTTTCCTAGGC.AAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGGGG...cccccc > 1:300995/1‑101 (MQ=255)
gCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCG.CTTTTCCTAGGC.AAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...CCCCCC...gc < 1:345728/101‑2 (MQ=255)
cAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCG.CTTTTCCTAGGC.AAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGGAG...caccccccc > 2:1156469/1‑98 (MQ=255)
aGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCG.CTTTTCCTAGGC.AAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGGAG...CGCCCC...gccg > 2:57291/1‑98 (MQ=255)
ccGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCG.CTTTTCCTAGGC.AAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGGAG...gccccccccggg > 2:2571934/1‑95 (MQ=255)
gCATGCCGCCCGCGTCAACAGGCAAGGGCCCCG.CTTTTCCTAGGC.AAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...CCCCCC...gcgggtcg < 2:1699102/101‑8 (MQ=255)
aTGCCGTCCGGGTCAACAGGCAAGGGCCCCG.CTTTTCCTAGGC.AAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...CCCCCC...gcgggtcgcc < 2:3460540/101‑10 (MQ=38)
aaCAGGCAAGGCCCCCG.TTTTTCCTAGGC.AAAATCCGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAC...CCCCCCGCGGG...T....CG..CCTCGGGCTTGCCCGa < 2:2401650/101‑1 (MQ=18)
ggggcaGGGCCCCG.TTTTTCCCTAGC.AAAAACCCCCCTTTTTTTTTTTGGGGGGGGGGGGGGGGGAAG...CCCCCC...CG...CGGGTCG..CCTCGGGCTTGCCCGAgg < 1:3802626/96‑1 (MQ=2)
aggccgggcccccG.CTTTCCATGGAA.AAAATGC.CCCTTTTTTTTTTGCGGGGGGGGGGGGGGATAAA...CCCCCC...GG...CGGGTCG..CCTCGGGCTTGCCCGAGGc < 2:2330100/92‑1 (MQ=2)
aaGGGCCCCG.CTTTTCCTAGGC.AAAATACGCCTTTTTTTCTTTGGGGGGGGGCGGGGGGGGAAC...CCCCCCGCGGG...T....CG..CCTCGGGCTTGCCCGAGGC.Gaaa < 2:970752/101‑1 (MQ=9)
aaGGGCCCCG.CTTTTCCTAGGC.AAAATACGCCCTTCTTTCTTTTCGGGGGGGTGGGGGGTGAAG...CCCCCCGCGGG...T....CG..CCTCGGGCTTGCCCGAGGC.Gaaa < 1:3505702/101‑1 (MQ=18)
gCCCCGTCTTTCCCTGGGA.AAAATCCCCCTTTCTTTTTTTGGGGGGGGGGGGGGGGGGACG...CCCCCC...GCGGGT....CG..CCTCGGGCTTGCCCGAGGC.GAAAGCt < 2:368073/101‑1 (MQ=2)
gCCCCG.CTTTTCCTAGGC.AAAATACGCCCTTCTTTCTTTGCGGGGGGGGGGGGGGTGAAG...CCCCCC...GCGGGT....CG..CCTCGGGCTTGCCCGAGGC.GAAAGCtt < 2:3313039/101‑1 (MQ=18)
ccG.CTTTCCCAAGGA.AAAATCCGCCTTTCTTTCTTTGGGGGGGGGCGGGGGGTGAAG...CCCCCC...GCGGGT....CG..CCTCGGGCTTGCCCGAGGC.GAAAGCTT.ccc < 1:1283984/101‑1 (MQ=2)
cccgttttCCTAAGCAAAAATCCCCCTTTTTTTTTTTTGGGGGGGGCGGGGGGTGAAC...CCCCCC...GC...GGGT.CG..CCTCGGGCTTGCCCGAGGC.GAAAGCTT.cc < 2:359166/97‑1 (MQ=2)
cG.CTTTTCCTAGGC.AAAATACCCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...CCCCCC...GC...GGGT.CG..CCTCGGGCTTGCCCGAGGC.GAAAGCTT.cccc < 2:1617701/101‑1 (MQ=18)
ttttCCTAGGC.AAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...CCCCCCGCGGG...T....CG..CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCg < 2:2785093/101‑1 (MQ=21)
tCCTAGGC.AAAATCCGCCCTTCTTTCTTTTCGGGGGGGGGGGGGGTGAAG...CCCCCCGCGGG...T....CG..CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGa < 1:3103737/101‑1 (MQ=18)
cTAGGA.AAAATCCGCCCTTTTTTCTTTCGGGGGGGGCGGGGGGTGAAC...CCCCCCGCGGG...T....CG..CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGaaa < 2:329583/101‑1 (MQ=9)
tac.AAAAAGCGCCGTTTTGTCTTTGGGGGGGGGGGGGGGGGGAAC...CCCCCCGCGGG...T....CG..CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAAcc < 1:1310269/99‑1 (MQ=2)
aTACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGAAGGCCCCCCCCG..GG...C....GG..CCCCGGGCTTCCCCGGGGG.GGAACTTT.CCCCCCCGCAAAACCTTCcc > 2:3522820/1‑101 (MQ=255)
tACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGAAGGC.CCCCCCG..GG...G....CG..CCCCCGGCGTGGCCCAGGCGGAAAGCTTCCCCCCCGTGaaacctgccct > 2:3430024/1‑93 (MQ=255)
ttctttTCGGGGGGGCGGGGGGGGGAGAC.CCCCCC...GG...G....GGGCCCCCGGGCTGGCCCGGGGG.GAAAGCTC.CCCCCCCGGAAAAAC.TGCCCGCGCGCGGCGcg > 2:2540037/1‑100 (MQ=255)
tttttgggggggggGGGGGGGTGACC...CCCCCCGCGGG...T....CG..CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCtt < 1:3167391/94‑1 (MQ=17)
tttCGGGGGGGCGGGGGGTGAGG...CCCCCCGCGGG...T....CG..GCCCAGGCCTGCCCCGGGC.GAAAGAGT.CCCCCCCCGAAAACCCCCCCCCCGGGCGTCGCCTTcc > 2:91638/1‑101 (MQ=255)
ggggggCGGGGGGGGACC...CCCCCCGCGGG...T....CG..CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTc < 1:341857/101‑1 (MQ=18)
ggggGGGGGGGTGAAC...CCCCCCGCGGG...T....CG..CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCgg < 1:3523073/101‑1 (MQ=21)
gCGGGGGGTGAAG...CCCCCCGCGGG...G....CG..CCTCGGGCCTGCCCGAGGC.GAAAGCCT.TCCCCCCTGCAAAACTTGCCCGCGCGCGTCGCTTTTCCTTTCGGccc > 2:2788887/1‑101 (MQ=12)
cGGGGGGTGAAG...CCCCCCGCGGG...T....CG..CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGGGAAAACCTTCCCTCCCCCCGCCCCTTTCCCTTTCGCCCCg > 1:4034232/1‑101 (MQ=9)
cGGGGGGTGAAG...CCCCCCGCGGG...G....CG..CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCCTCGCTTTCCCTTTCGGCCCg > 2:4157530/1‑101 (MQ=21)
ggggggTGAAG...CCCCCCGCGGG...T....CG..CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCgg > 2:3048376/1‑101 (MQ=21)
ggggggTGAAG...CCCCCCGCGGG...T....CG..CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCgg > 1:3420310/1‑101 (MQ=21)
ggggggTGAAG...CCCCCCGCGGG...T....CG..CCTCGGGCTTGCCCGAGGC.GAAAACTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGccccg > 1:3548913/1‑99 (MQ=21)
gggggTGAAG...CCCCCCGCGGG...T....CG..CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCGgc < 2:3477174/101‑1 (MQ=21)
|||
CTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCG.CTTTTCCTAGGC.AAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...CCCCCC...GT...C....CG..CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCGGC > NC_014034/1732766‑1732948
Base quality scores:
ATCG
< 3 ≤
ATCG
< 8 ≤
ATCG
< 18 ≤
ATCG
< 31 ≤
ATCG
< 40 ≤
ATCG
|