BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,740,769	+G	97.3%	intergenic (+6/+129)	cxp → / ← RCAP_rcc01610	thermostable carboxypeptidase 1/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,740,769	1	.→G	97.3%	4.5 ‑1.0 ‑0.7	147	intergenic (+6/+129)	cxp/RCAP_rcc01610	thermostable carboxypeptidase 1/hypothetical protein
Reads supporting (aligned to +/- strand): new base (73/70): ref base (1/3): total (74/73)
Fisher's exact test strand distribution p-value = 3.66e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.79e-01

Only 100 of 183 total aligned reads displayed.


GCGAGACGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.A.GGG.G.CGCTGCCCCCCG.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGG  >  NC_014034/1740669‑1740864


gcgAGACGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.a                                                                                                     >  2:1873893/1‑101 (MQ=255)


 ggagaCGATCGGGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.A.g                                                                                                   <  2:3572054/100‑1 (MQ=255)


     aCGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.A.ggg.gg                                                                                              <  2:2330408/101‑2 (MQ=255)


      ctaTCGTGCCCGCCTGGGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.A.ggg.ggc                                                                                             <  2:2813320/99‑3 (MQ=255)


      cGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.A.ggg.ggc                                                                                             <  1:434329/101‑3 (MQ=255)


       gATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.A.ggg.ggcg                                                                                            >  1:1763222/1‑98 (MQ=255)


         tCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.A.ggg.ggcgct                                                                                          <  2:1171833/101‑6 (MQ=255)


          cGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTg                                                                                         >  1:2714528/1‑101 (MQ=255)


          cGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTg                                                                                         >  1:1999884/1‑101 (MQ=255)


           gTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTATGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGc                                                                                        >  2:670872/1‑101 (MQ=255)


            ttacggCCTGCGGGGTCGAGCGGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATAAGCCCCGATCTACCGGCTCTGAGGGC.G.ACGGC.G.CCCTGcc                                                                                       >  2:4079501/6‑101 (MQ=255)


             gACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGccc                                                                                      >  2:4150600/1‑101 (MQ=255)


               ccGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGccccc                                                                                    <  2:4227181/101‑1 (MQ=255)


               ccGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGccccc                                                                                    <  1:2145130/101‑1 (MQ=255)


                     gCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAg                                                                             >  1:684484/1‑101 (MQ=255)


                      ggggTGCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAgg                                                                            <  2:966505/100‑1 (MQ=255)


                       gggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGc                                                                           >  2:3022203/1‑101 (MQ=255)


                        gtttCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCg                                                                          <  2:1100508/99‑1 (MQ=255)


                        ggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCg                                                                          >  2:2167366/1‑101 (MQ=255)


                         gTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGa                                                                         >  2:2928966/1‑101 (MQ=255)


                           tggAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATg                                                                       <  1:226619/99‑1 (MQ=255)


                           tCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.GGCTGCCCCCCC.CGAGGCGGTg                                                                       >  1:1849954/1‑101 (MQ=255)


                             gAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGcc                                                                     <  2:3114096/101‑1 (MQ=255)


                              aGCCGAGGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCt                                                                    <  1:1658539/101‑1 (MQ=255)


                               gCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTc                                                                   <  1:2929188/101‑1 (MQ=255)


                                 cGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCgg                                                                 >  2:4106053/1‑101 (MQ=255)


                                    cGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGccc                                                              <  1:2968041/101‑1 (MQ=255)


                                     ggAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGcccc                                                             >  1:258382/1‑101 (MQ=255)


                                       aagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGcccccc                                                           <  2:900953/101‑1 (MQ=255)


                                        agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCg                                                          >  1:4230565/1‑101 (MQ=255)


                                        agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCg                                                          >  1:340001/1‑101 (MQ=255)


                                        agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCg                                                          <  2:971158/101‑1 (MQ=255)


                                         gacccGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCgg                                                         <  2:3707483/98‑1 (MQ=255)


                                         gaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.T.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCgg                                                         >  1:1041066/1‑101 (MQ=255)


                                         gaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCgg                                                         >  1:3304711/1‑101 (MQ=255)


                                          aaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTCCCGGCTTTGAGGGCGG.G.GGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCggg                                                        <  2:484816/101‑1 (MQ=255)


                                          aaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCggg                                                        <  1:3245291/101‑1 (MQ=255)


                                          aaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCcgg                                                        >  2:3234423/1‑101 (MQ=255)


                                          aaCCGCTTCTGGACTATCCCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCggg                                                        <  2:2018048/101‑1 (MQ=255)


                                            ccGTTTCTGGATTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGat                                                      <  2:1054124/101‑1 (MQ=255)


                                             cGCTTCTGGACTATCTGGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGata                                                     <  2:2688822/101‑1 (MQ=38)


                                              gCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGatat                                                    >  1:3008572/1‑101 (MQ=255)


                                              gCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGatat                                                    >  2:3642470/1‑101 (MQ=255)


                                               cTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATCtt                                                   <  1:2419941/101‑1 (MQ=255)


                                               cTTCTGGACTACCTCGAAAGCAAATACGCCGCGATGTACCGGCTTGGGGGGC.GCG.GGG.G.CGC.GCCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATAtt                                                   <  1:2816891/101‑1 (MQ=25)


                                                ttCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAttt                                                  <  2:3711395/101‑1 (MQ=38)


                                                 tCTGGACTATCTCGAAAGCAAATCCGCCGCATTCTACCGGCTTTGAGGGC.T.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                 <  2:1040972/101‑1 (MQ=25)


                                                  cTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCg                                                >  1:922239/1‑101 (MQ=38)


                                                  cTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCg                                                >  2:3990633/1‑101 (MQ=38)


                                                   tGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGa                                               >  1:4138273/1‑101 (MQ=38)


                                                   tGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.A.GGG.GTCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGa                                               >  2:557739/1‑101 (MQ=38)


                                                      aCTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGA.CGAAGCGATGCCTCGGCCCCCCGGGATATTTCGAGCa                                            >  1:3618172/1‑101 (MQ=38)


                                                      aCTACCCCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCa                                            <  2:3798135/101‑1 (MQ=37)


                                                        tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                          >  1:859885/1‑101 (MQ=38)


                                                        tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                          <  1:112175/101‑1 (MQ=38)


                                                        tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                          >  1:2540168/1‑101 (MQ=38)


                                                          tctcGAAAGCAAATACCCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGAt                                        <  1:2206831/101‑1 (MQ=37)


                                                           ctcGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGG.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATg                                       <  1:3928573/101‑1 (MQ=37)


                                                           ctcGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATg                                       >  2:1145487/1‑101 (MQ=37)


                                                            tcGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGa                                      <  2:2570851/101‑1 (MQ=37)


                                                             cGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaa                                     >  1:3235464/1‑101 (MQ=37)


                                                              gAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                    >  2:727967/1‑101 (MQ=37)


                                                              gAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                    >  1:3278692/1‑101 (MQ=37)


                                                                 aGCAAATCCGCCGCGATCTCCCGGCTTGGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGc                                 <  2:818173/101‑1 (MQ=25)


                                                                 aGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGc                                 <  1:1874013/101‑1 (MQ=38)


                                                                   cAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGTTGAAAGGCAt                               >  2:1783908/1‑101 (MQ=37)


                                                                   cAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCAt                               <  2:2243810/101‑1 (MQ=38)


                                                                   cAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCAt                               >  1:1056380/1‑101 (MQ=37)


                                                                   cAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCAt                               <  1:1251807/101‑1 (MQ=38)


                                                                    aaaTACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATc                              >  1:1945753/1‑101 (MQ=37)


                                                                     aaTACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATcc                             >  2:3287686/1‑101 (MQ=37)


                                                                     aaTACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGA.CGAGGCGATACCTCGGCCCCCCGGGATATTTCGAGCAAAATGAAAGACagat                             >  2:1394095/1‑98 (MQ=37)


                                                                      aTACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCg                            >  2:1364569/1‑101 (MQ=37)


                                                                      aTACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCg                            >  1:1303037/1‑101 (MQ=37)


                                                                      aTACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCACCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCg                            >  1:3621192/1‑101 (MQ=37)


                                                                        aCGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGt                          <  2:3242979/101‑1 (MQ=38)


                                                                         cgccgcGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGtt                         <  2:2148817/101‑1 (MQ=38)


                                                                          gccgcGATCTCCCGCCTTTGAGGGCGG.G.GGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGttt                        <  1:2324184/101‑1 (MQ=25)


                                                                          gccgcGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGttt                        >  2:861315/1‑101 (MQ=37)


                                                                          gccgcGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGttt                        <  2:2796992/101‑1 (MQ=37)


                                                                          gccgcGATCTACCGGCTTCGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGttt                        >  2:1131882/1‑101 (MQ=37)


                                                                           ccgcGATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTc                       >  1:213608/1‑101 (MQ=37)


                                                                             gcgATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCAt                     >  1:1903926/1‑101 (MQ=37)


                                                                              cgATCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATc                    >  1:3940420/1‑101 (MQ=37)


                                                                               gATCTCCCGGCTTTGAGGGC.G.A.GGGTG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCt                   <  2:213570/101‑1 (MQ=25)


                                                                                aTCTACGGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCtt                  <  2:26625/101‑1 (MQ=25)


                                                                                 tCTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTg                 >  1:352956/1‑101 (MQ=25)


                                                                                  cTACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGc                >  1:2073396/1‑101 (MQ=25)


                                                                                   tcccGGCTTTGGGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCt               <  1:160894/99‑1 (MQ=21)


                                                                                   tACCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCt               >  2:3524046/1‑101 (MQ=25)


                                                                                    cccGGCTTTGGGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCtt              <  1:419360/100‑1 (MQ=25)


                                                                                    aCCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCtt              <  1:524066/101‑1 (MQ=25)


                                                                                    aCCGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCtt              >  2:2722815/1‑101 (MQ=25)


                                                                                     ccGGCTTTGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTc             >  1:899177/1‑101 (MQ=25)


                                                                                            tGAGGGC.G.AGGGG.G.CGCTTCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAcc      <  1:1404471/101‑1 (MQ=21)


                                                                                            tGAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAcc      >  1:692239/1‑101 (MQ=25)


                                                                                             gAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCt     >  2:3968906/1‑101 (MQ=25)


                                                                                             gAGGGC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCt     >  1:313822/1‑101 (MQ=25)


                                                                                               gggC.G.AGGGG.G.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCg   <  2:1999758/101‑1 (MQ=25)


                                                                                                ggC.G.A.GGG.GCCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCgg  <  2:1903806/101‑1 (MQ=25)


GCGAGACGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.A.GGG.G.CGCTGCCCCCCG.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGG  >  NC_014034/1740669‑1740864

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 23 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG