Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,740,785 +C 3.8% intergenic (+22/+113) cxp → / ← RCAP_rcc01610 thermostable carboxypeptidase 1/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,740,7851.→C 3.8% 11.8 ‑0.4 ‑0.2157intergenic (+22/+113)cxp/RCAP_rcc01610thermostable carboxypeptidase 1/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/4):  ref base (73/78):  total (75/82)
Fisher's exact test strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.70e-01

Only 100 of 168 total aligned reads displayed.

GCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.GGG.G.CG.CTGCCCCCCG.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGG  >  NC_014034/1740690‑1740880
                                                                                                     |                                                                                               
 cGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAgg                                                                                            >  1:859951/1‑101 (MQ=255)
  gggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGc                                                                                           >  2:3022203/1‑101 (MQ=255)
  gggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGc                                                                                           >  1:2976942/1‑101 (MQ=255)
   ggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCg                                                                                          >  2:2167366/1‑101 (MQ=255)
    gTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGa                                                                                         >  2:2928966/1‑101 (MQ=255)
      tggAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATg                                                                                       <  1:226619/99‑1 (MQ=255)
        gAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGcc                                                                                     <  2:3114096/101‑1 (MQ=255)
        gAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTGGAACGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGcc                                                                                     >  1:3617127/1‑101 (MQ=255)
         aGCCGAGGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCt                                                                                    <  1:1658539/101‑1 (MQ=255)
               cGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.GG.CTGCCCCCGC.CGAGGCGATGCCCCGGccc                                                                              >  1:2952982/1‑101 (MQ=255)
               cGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGccc                                                                              <  2:3742102/101‑1 (MQ=255)
                ggAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGcccc                                                                             >  1:258382/1‑101 (MQ=255)
                  aagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGcccccc                                                                           <  1:3323174/101‑1 (MQ=255)
                   agaaCCGCTTTTGGACTACCTCGAAATCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCg                                                                          <  2:1849834/101‑1 (MQ=255)
                   agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCg                                                                          >  1:4230565/1‑101 (MQ=255)
                   agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCg                                                                          <  1:2914533/101‑1 (MQ=255)
                   agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCg                                                                          >  2:2404444/1‑101 (MQ=255)
                   agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCg                                                                          <  2:971158/101‑1 (MQ=255)
                    gaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.TAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCgg                                                                         >  1:1041066/1‑101 (MQ=255)
                     aaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTCCCGGCTTTGAGGGCGGG.GGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCggg                                                                        <  2:484816/101‑1 (MQ=255)
                     aaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCcgg                                                                        >  2:3234423/1‑101 (MQ=255)
                     aaCCGCTTCTGGACTATCTCGAAAGCAAATACCCCGCGATCTACCGGCTTGGAGGGC.GG.GGG.GCCG.CTCCCCCCGC.CGAGGCGATGCCTCGGCCCCCCgga                                                                        <  2:1155287/101‑2 (MQ=255)
                       ccGTTTCTGGATTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGat                                                                      <  2:1054124/101‑1 (MQ=255)
                        cGCTTCTGGACTATCTGGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGata                                                                     <  2:2688822/101‑1 (MQ=38)
                        cGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGata                                                                     >  1:3711649/1‑101 (MQ=255)
                          cTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATCtt                                                                   <  1:2419941/101‑1 (MQ=255)
                          cTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAtt                                                                   >  1:3033267/1‑101 (MQ=38)
                           ttCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAttt                                                                  <  2:3711395/101‑1 (MQ=38)
                            tCTGGACTATCTCGAAAGCAAATCCGCCGCATTCTACCGGCTTTGAGGGC.TAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                                 <  2:1040972/101‑1 (MQ=25)
                            tCTGGACTATCTCGAAAGCAAATACGCCGCGATCTCCCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                                 <  2:3972914/101‑1 (MQ=38)
                            tCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                                 >  1:1959936/1‑101 (MQ=38)
                            tCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                                 >  1:3412729/1‑101 (MQ=38)
                             cTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCg                                                                >  1:922239/1‑101 (MQ=38)
                             cTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCg                                                                >  2:3990633/1‑101 (MQ=38)
                             cTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCg                                                                <  1:691105/101‑1 (MQ=38)
                               ggACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAg                                                              <  2:2688454/101‑1 (MQ=38)
                                gACTATCTCGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGc                                                             <  2:825758/101‑1 (MQ=38)
                                 aCTATCTCGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCa                                                            <  2:4172750/101‑1 (MQ=38)
                                   tATCTCGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                                          <  1:1268678/101‑1 (MQ=37)
                                   tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                                          >  1:2540168/1‑101 (MQ=38)
                                   tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                                          >  2:4091204/1‑101 (MQ=38)
                                   tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                                          <  1:69834/101‑1 (MQ=38)
                                   tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                                          >  1:859885/1‑101 (MQ=38)
                                   tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGAACTTTCGAGCAAg                                                          >  1:668402/1‑101 (MQ=255)
                                    aTCTCGAAAGCAAATACGCCGCGATCTCCCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGa                                                         <  1:3291618/101‑1 (MQ=37)
                                    aTCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGa                                                         >  1:1812148/1‑101 (MQ=38)
                                     tttcGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGAt                                                        >  2:647378/3‑101 (MQ=37)
                                     tctcGAAAGCAAATACCCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGAt                                                        <  1:2206831/101‑1 (MQ=37)
                                      ctcGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCatcatg                                                       <  2:3581644/101‑6 (MQ=37)
                                       tcGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGa                                                      <  2:2570851/101‑1 (MQ=37)
                                        cGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaa                                                     <  2:1100913/101‑1 (MQ=37)
                                        cGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaa                                                     >  1:3235464/1‑101 (MQ=37)
                                         gAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                                    >  1:3932819/1‑101 (MQ=37)
                                         gAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                                    >  1:3278692/1‑101 (MQ=37)
                                         gAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                                    >  2:727967/1‑101 (MQ=37)
                                            atctaatgctccgcGATCTCCCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGc                                                 <  1:1394194/91‑1 (MQ=25)
                                            aGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGc                                                 <  1:1874013/101‑1 (MQ=38)
                                              cAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCAt                                               <  1:1251807/101‑1 (MQ=38)
                                              cAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCAt                                               >  1:1056380/1‑101 (MQ=37)
                                               aaaTACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATc                                              >  1:2588114/1‑101 (MQ=37)
                                                aaTACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGA.CGAGGCGATACCTCGGCCCCCCGGGATATTTCGAGCAAAATGAAAGACagat                                             >  2:1394095/1‑98 (MQ=37)
                                                 aTACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCACCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCg                                            >  1:3621192/1‑101 (MQ=37)
                                                  tACGCCGCGCTCTACCGGGTTTGAGGGC.GA.GGG.GCCG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCgg                                           <  2:3881854/101‑1 (MQ=37)
                                                   aCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGt                                          <  2:3242979/101‑1 (MQ=38)
                                                    cgccgcGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGtt                                         <  2:2148817/101‑1 (MQ=38)
                                                     gccgcGATCTCCCGCCTTTGAGGGCGGG.GGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGttt                                        <  1:2324184/101‑1 (MQ=25)
                                                     gccgcGATCTACCGGCTTCGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGttt                                        >  2:1131882/1‑101 (MQ=37)
                                                      ccgcGATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTc                                       >  1:213608/1‑101 (MQ=37)
                                                         cgATCTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATc                                    >  1:3940420/1‑101 (MQ=37)
                                                          gATCTCCCGGCTTTGAGGGC.GA.GGGTG.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCt                                   <  2:213570/101‑1 (MQ=25)
                                                           aTCTACGGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCtt                                  <  2:26625/101‑1 (MQ=25)
                                                             cTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGc                                >  1:2073396/1‑101 (MQ=25)
                                                             cTACCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCAGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGc                                >  1:4174680/1‑101 (MQ=25)
                                                              tcccGGCTTTGGGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCt                               <  1:160894/99‑1 (MQ=21)
                                                               cccGGCTTTGGGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCtt                              <  1:419360/100‑1 (MQ=25)
                                                               aCCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCtt                              >  2:2722815/1‑101 (MQ=25)
                                                               aCCGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCtt                              <  1:524066/101‑1 (MQ=25)
                                                                 cGGCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCa                            <  1:1859134/101‑1 (MQ=25)
                                                                  ggCTTTGAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCaa                           <  2:3687779/101‑1 (MQ=25)
                                                                    gttggAGGGG.GA.GGG.G.CGCCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAAt                         <  2:3533108/97‑1 (MQ=21)
                                                                     tttGAGGGG.GGGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATa                        <  2:870876/101‑1 (MQ=21)
                                                                      ttGAGGGC.GAGGGG.G.CC.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAc                       <  2:1366725/101‑1 (MQ=21)
                                                                      tggAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAc                       <  2:928086/99‑1 (MQ=21)
                                                                        gAGGGC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCt                     >  1:313822/1‑101 (MQ=25)
                                                                         gggggggagggg.g.CT.CCGCCCCCGC.CGAGGCGAAGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCt                     <  1:3653050/92‑1 (MQ=18)
                                                                         ggggC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTc                    <  2:1959810/100‑1 (MQ=25)
                                                                          gggC.GAGGGG.G.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCg                   <  2:1999758/101‑1 (MQ=25)
                                                                             gcgggggg.g.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCggg                 <  2:3470952/96‑1 (MQ=255)
                                                                              cgagggg.g.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCgggg                >  1:2810754/5‑101 (MQ=21)
                                                                               gagggg.g.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCggggg               >  1:1152371/4‑101 (MQ=255)
                                                                               gagggg.g.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCggggg               >  2:1095389/4‑101 (MQ=255)
                                                                                agggg.g.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCgggggg              >  1:1938647/3‑101 (MQ=255)
                                                                                 gggg.g.CG.CTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGa             <  2:888438/100‑1 (MQ=255)
                                                                                   gg.g.CG.CTCCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGt           <  2:2052986/101‑1 (MQ=255)
                                                                                        gcccggCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTcc         <  1:2482224/96‑1 (MQ=255)
                                                                                           cctccccccGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCg       <  2:4216505/98‑1 (MQ=255)
                                                                                           cTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGc      >  2:308430/1‑101 (MQ=255)
                                                                                             gCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGccg    >  1:3483330/1‑99 (MQ=255)
                                                                                                ccccGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAggg  >  1:4026792/1‑101 (MQ=255)
                                                                                                ccccGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAggg  >  1:2929481/1‑101 (MQ=255)
                                                                                                     |                                                                                               
GCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.GGG.G.CG.CTGCCCCCCG.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGG  >  NC_014034/1740690‑1740880

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG