Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,740,893 +C 97.5% intergenic (+130/+5) cxp → / ← RCAP_rcc01610 thermostable carboxypeptidase 1/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,740,8931.→C97.5% 4.3 0.0 ‑0.5163intergenic (+130/+5)cxp/RCAP_rcc01610thermostable carboxypeptidase 1/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (87/72):  ref base (2/2):  total (89/74)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.23e-01

Only 100 of 194 total aligned reads displayed.

GCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CG.C.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGCCAGGGTCAGGGGT  >  NC_014034/1740795‑1740988
                                                                                                    |                                                                                                 
gCCGCGGCGCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.GG.c.c                                                                                                <  2:1763103/101‑1 (MQ=255)
  cTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CG.c.c.cc                                                                                             >  2:2424673/1‑101 (MQ=255)
   tCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CG.c.c.ccc                                                                                            >  1:3538054/1‑101 (MQ=255)
   tCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CG.c.c.ccc                                                                                            <  1:4106314/101‑1 (MQ=255)
     ggCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CG.c.c.cccct                                                                                          >  1:1890041/1‑99 (MQ=255)
     ggCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGCGACGGGGGGCAG.CG.c.c.ccccc                                                                                          >  2:528209/1‑99 (MQ=255)
      gCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CG.c.c.ccccta                                                                                         >  2:816985/1‑98 (MQ=255)
      gCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGAAGTCCCGCAGGGACGGGGGGCAG.CG.c.c.ccccta                                                                                         >  1:2047553/1‑98 (MQ=255)
       ccccccGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CG.c.c.cccctac                                                                                        >  1:733599/1‑97 (MQ=255)
       ccccccGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CG.c.c.cccctac                                                                                        <  2:4200371/101‑5 (MQ=255)
        cccccGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CG.c.c.cccctacg                                                                                       <  2:3235223/101‑6 (MQ=255)
        cccccGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CG.c.c.cccctacg                                                                                       >  1:2660219/1‑96 (MQ=255)
        cccccGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CG.c.c.cccctacg                                                                                       <  1:2815684/101‑6 (MQ=255)
         ccccGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGc                                                                                       >  2:1911712/1‑101 (MQ=255)
            cGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCAt                                                                                    <  2:4170656/101‑1 (MQ=255)
             gggATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCAtt                                                                                   <  1:1145581/101‑1 (MQ=255)
             gggATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCAtt                                                                                   <  2:2539977/101‑1 (MQ=255)
               gATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGa                                                                                 >  2:352705/1‑101 (MQ=255)
                atatTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGAc                                                                                <  2:3620939/101‑1 (MQ=255)
                atatTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGCAGTGCTGCAGGGACGGGAGGCAG.TG.CTC.CCCTACGCCATTGAc                                                                                >  2:2688260/1‑101 (MQ=21)
                 tatTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACg                                                                               >  2:1338087/1‑101 (MQ=255)
                  atTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCGGCAGGGACGGGGGGCAG.CG.C.CGCCCCACGCCATTGCCGt                                                                              >  2:2377889/1‑101 (MQ=21)
                  atTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACgg                                                                              >  2:3612581/1‑100 (MQ=255)
                   tttCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTg                                                                             >  2:1887228/1‑101 (MQ=255)
                     tCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGaa                                                                           <  2:2535759/101‑1 (MQ=255)
                      cGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAg                                                                          >  2:3420974/1‑101 (MQ=255)
                      cGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAg                                                                          >  2:1222229/1‑101 (MQ=255)
                        aGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCg                                                                        <  1:995762/101‑1 (MQ=255)
                         gCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCgg                                                                       <  2:3932559/101‑1 (MQ=255)
                         gCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCgg                                                                       <  2:1938521/101‑1 (MQ=255)
                         gCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCgg                                                                       >  1:2923201/1‑101 (MQ=255)
                           aaGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGtt                                                                     >  1:1159581/1‑101 (MQ=255)
                           aaGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGtt                                                                     >  1:1120100/1‑101 (MQ=255)
                           aaGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGtt                                                                     >  1:4220534/1‑101 (MQ=255)
                            aGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTc                                                                    <  2:222085/101‑1 (MQ=255)
                             gATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCa                                                                   <  1:4009579/101‑1 (MQ=255)
                             gATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCa                                                                   <  2:339950/101‑1 (MQ=255)
                             gATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCa                                                                   <  2:733523/101‑1 (MQ=255)
                              aTGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCAc                                                                  >  2:873127/1‑101 (MQ=255)
                               tGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCAcc                                                                 >  1:1290263/1‑101 (MQ=255)
                                gAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCg                                                                >  1:1564117/1‑101 (MQ=255)
                                gAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCg                                                                >  1:1691218/1‑101 (MQ=255)
                                gAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCg                                                                >  1:1833998/1‑101 (MQ=255)
                                gAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCg                                                                <  2:2976720/101‑1 (MQ=255)
                                  aaGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGc                                                              <  1:3026608/101‑1 (MQ=255)
                                  aaGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGc                                                              >  1:1512900/1‑101 (MQ=255)
                                       aTCGGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGtctc                                                         >  2:2769519/1‑101 (MQ=255)
                                       aTCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGtctc                                                         <  2:3008347/101‑1 (MQ=255)
                                        tCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCg                                                        >  1:2616000/1‑101 (MQ=255)
                                        tCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCg                                                        <  1:647455/101‑1 (MQ=255)
                                         ccGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGa                                                       >  1:3087172/1‑101 (MQ=255)
                                          cGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGAt                                                      >  2:2453557/1‑101 (MQ=255)
                                           ggTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATc                                                     <  2:3617919/101‑1 (MQ=255)
                                           ggTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATc                                                     >  1:3403740/1‑101 (MQ=255)
                                             tttCATCTTGCTTCAAATAGCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCAc                                                   <  1:3955649/101‑1 (MQ=255)
                                             tttCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCAc                                                   >  2:1077985/1‑101 (MQ=255)
                                             tttCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCAc                                                   >  2:1969163/1‑101 (MQ=255)
                                               tCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGc                                                 <  2:1454294/101‑1 (MQ=255)
                                               tCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGc                                                 >  2:3299922/1‑101 (MQ=255)
                                               tCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGc                                                 >  2:4034497/1‑101 (MQ=255)
                                                cATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCt                                                >  2:1495652/1‑101 (MQ=255)
                                                cATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCt                                                >  1:2034604/1‑101 (MQ=255)
                                                cATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCt                                                >  1:1217111/1‑101 (MQ=255)
                                                   cTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGTGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCtttt                                             <  1:3642723/101‑1 (MQ=255)
                                                     tGCTTCAAATCCCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTg                                           <  1:1078079/101‑1 (MQ=255)
                                                      gCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGa                                          <  2:3537801/101‑1 (MQ=255)
                                                      gCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGa                                          >  1:361781/1‑101 (MQ=255)
                                                       cTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGAc                                         >  1:801988/1‑101 (MQ=255)
                                                          cAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGAcggc                                      >  1:503178/1‑101 (MQ=255)
                                                          cAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGAcggc                                      >  1:3880241/1‑101 (MQ=255)
                                                            aaTACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGAcggcgg                                    <  1:1661595/101‑1 (MQ=255)
                                                            aaTACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGAcggcgg                                    >  1:3241/1‑101 (MQ=255)
                                                                ccTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTc                                <  2:2615809/101‑1 (MQ=255)
                                                                 cTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCt                               >  1:3003981/1‑101 (MQ=255)
                                                                 cTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCt                               >  1:3642905/1‑101 (MQ=255)
                                                                 cTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCt                               <  2:3033042/101‑1 (MQ=255)
                                                                    ggggggAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCAGGCTTTTTGACGGCGGCGTCTggg                            >  1:1778570/1‑101 (MQ=255)
                                                                    ggggggAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTggg                            >  2:3572647/1‑101 (MQ=255)
                                                                    ggggggAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTGTGACGGCGGCGTCTggg                            >  2:3806870/1‑101 (MQ=255)
                                                                       gggAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGa                         >  1:3508713/1‑101 (MQ=255)
                                                                       gggAGTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGa                         <  2:1302941/101‑1 (MQ=255)
                                                                        ggAGCCCCCCAGGAGGGGGGGGCGGCCC.C.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGaa                        <  1:2453741/101‑1 (MQ=255)
                                                                          tgTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAgc                      <  1:2721222/100‑1 (MQ=255)
                                                                           gTCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGGTCACCGGCGACTCGATCACGCTTTGTGACGGCGGCGTCTGGGCAAAgcg                     >  2:571163/1‑101 (MQ=37)
                                                                            tCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAgcgc                    >  2:1185573/1‑101 (MQ=255)
                                                                            tCCCGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAgcgc                    >  1:1670253/1‑101 (MQ=255)
                                                                             cccGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCt                   >  2:2866435/1‑101 (MQ=255)
                                                                             cccGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCt                   <  2:2140379/101‑1 (MQ=255)
                                                                              ccGCAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTc                  <  1:1083209/101‑1 (MQ=255)
                                                                                 cAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGGTCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGc               >  1:558593/1‑101 (MQ=255)
                                                                                 cAGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGc               >  2:3043380/1‑101 (MQ=255)
                                                                                  aGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGcc              >  1:2545988/1‑101 (MQ=255)
                                                                                  aGGGACGGGGGGCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGcc              <  1:1969289/101‑1 (MQ=255)
                                                                                      agggggggCAGCCC.C.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGAGGGCGGCGTCTGGGCGAAGCGCTCGGCCAggg          <  2:4131105/99‑1 (MQ=255)
                                                                                        ggggggCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGCCAGGGTc        <  1:752957/101‑1 (MQ=255)
                                                                                         gggggCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGCCAGGGTCa       <  2:2034466/101‑1 (MQ=255)
                                                                                         gggggCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGCCAGGGTCa       >  1:3917061/1‑101 (MQ=255)
                                                                                          ggggCAGCCC.C.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGCCAGGGTCAg      <  2:1512796/101‑1 (MQ=255)
                                                                                             gCAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGCCAGGGTCAgggg   <  2:1852854/101‑1 (MQ=255)
                                                                                              cAG.CGCC.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGCCAGGGTCAGGGGt  <  2:4220272/101‑1 (MQ=255)
                                                                                                    |                                                                                                 
GCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG.CG.C.C.CCCTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGCCAGGGTCAGGGGT  >  NC_014034/1740795‑1740988

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG