BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,745,454	+C	100%	intergenic (‑114/+654)	RCAP_rcc01613 ← / ← metH4	hypothetical protein/methionine synthase subunit B

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,745,454	1	.→C	100.0%	100.0	137	intergenic (‑114/+654)	RCAP_rcc01613/metH4	hypothetical protein/methionine synthase subunit B
Reads supporting (aligned to +/- strand): new base (72/35): ref base (4/26): total (76/61)
Fisher's exact test strand distribution p-value = 1.42e-07
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.51e-02

Only 100 of 167 total aligned reads displayed.


GCAGCGCCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.G.GCGCGAGCTTTGGCA.CCT.C.A.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGAAGCGCAAGGCGC  >  NC_014034/1745354‑1745549


gCAGCGCCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.a                                                                                                  <  1:4097204/101‑1 (MQ=255)


gCAGCGCCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.a                                                                                                  <  1:82995/101‑1 (MQ=255)


 cAGCGCCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGGGAGC.TTGGCA.CCT.C.A.c                                                                                                >  1:686108/1‑101 (MQ=255)


 cAGCGCCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.A.c                                                                                                <  1:4214640/101‑1 (MQ=255)


 cAGCGCCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.A.c                                                                                                <  1:1419920/101‑1 (MQ=255)


    cgttccGGTTCGGGGGGCTGGGGTCCGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCTATCGCGGCGGTCGGC.GCGTGCGAGC.TTGGCA.CCT.C.A.cccc                                                                                             <  2:1964033/97‑1 (MQ=255)


     gcCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.A.ccccc                                                                                            <  1:1546311/101‑1 (MQ=255)


      ccccGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.A.cccccc                                                                                           >  1:236178/1‑101 (MQ=255)


       cccGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.A.ccccccc                                                                                          <  1:3219135/101‑1 (MQ=255)


        ccGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.A.cccccccc                                                                                         <  1:2194527/101‑1 (MQ=255)


        ccGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.A.cccccccc                                                                                         <  1:2017109/101‑1 (MQ=255)


          ggTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.A.cccccccccc                                                                                       >  2:1897990/1‑99 (MQ=255)


            ggcggggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.C.ccccccccccgg                                                                                     <  2:331866/100‑5 (MQ=255)


                gggggcgggggTTGGCAGGCCGGCCGTCTGATCGGGCAGCGGCATGTGGATCGCAGCGGTCGAC.GCCCGCGAGCTTTGG.T.CCT.CGC.CCCCCCCCGGCGCAc                                                                                  <  2:701558/101‑1 (MQ=255)


                gggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCAc                                                                                  <  2:3001614/101‑1 (MQ=255)


                 ggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCAcc                                                                                 <  1:89035/101‑1 (MQ=255)


                  gggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCTCC.C.CCCCCCCCGGCGCACCg                                                                                <  2:894738/101‑1 (MQ=255)


                  gggcgggggTCGGCAGCCCGGCCGGCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCg                                                                                <  2:2418221/101‑1 (MQ=255)


                   ggggggggTCGGCAGCCCGGCCGTGGGATCGGCCAGCGGCATGTCGATCGCGTCGGTCGCC.GCGCGCGAGC.TTGGCA.CCTCC.C.CCCCCCCCGGCGCACCgg                                                                               <  2:416343/98‑1 (MQ=255)


                     cgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCCGCGCACCCGcc                                                                             >  1:1074006/1‑100 (MQ=255)


                      gggggTCGGCAGCCGGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGcac                                                                            <  1:4019409/101‑1 (MQ=255)


                      gggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCCCCGGcac                                                                            >  2:3093889/1‑101 (MQ=255)


                        gggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAg                                                                          <  2:2146014/101‑1 (MQ=255)


                        gggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAg                                                                          <  1:4128866/101‑1 (MQ=255)


                         ggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGc                                                                         <  2:1623558/101‑1 (MQ=255)


                         ggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCCGCGCCCCGGCACCGc                                                                         >  2:3489184/1‑101 (MQ=255)


                           gcGGCAGCCCGGCCGGCGGAGCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGG.GCGCGAGC.TTGGCC.CCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCa                                                                       <  1:1056139/100‑1 (MQ=255)


                            cGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGGGCCCCGGCCAAGCCag                                                                      >  2:1802824/1‑101 (MQ=255)


                             ggCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCaga                                                                     >  1:1224226/1‑101 (MQ=255)


                             ggCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGGCGAC.GCGCGCGAGC.TTGGGA.CCT.C.ACCCCCCCCCCGCGCCCCGGCCCAGCCcga                                                                     >  1:3782817/1‑101 (MQ=255)


                              gCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAG.GTTGGCA.CCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCagag                                                                    <  1:3000299/101‑1 (MQ=255)


                               cAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCagaga                                                                   >  1:332056/1‑101 (MQ=255)


                                 gCCGGGCCGTCGGAGCGGCCAGGGTCATGTTGTTCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCCC.C.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAgc                                                                 <  2:3457830/101‑1 (MQ=255)


                                 gCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAgc                                                                 >  2:2201777/1‑101 (MQ=255)


                                 gCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCCGCGCACCGGCCCAGCCCGAGAgc                                                                 >  1:1590045/1‑101 (MQ=255)


                                  ccgggCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TGGGCA.CCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAgcg                                                                <  2:3176207/98‑1 (MQ=255)


                                  cccGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAgcg                                                                <  1:988824/101‑1 (MQ=255)


                                   ccGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAgcgc                                                               <  2:794066/101‑1 (MQ=255)


                                   ccGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAgcgc                                                               <  1:709823/101‑1 (MQ=255)


                                    cGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGcgcg                                                              <  1:2011180/101‑1 (MQ=255)


                                      gCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCgcggc                                                            <  1:2624826/101‑1 (MQ=255)


                                          tCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCCCCGGCACAGCCAGAGAGCGCGGCGGcc                                                        >  1:1654987/1‑101 (MQ=255)


                                            ggATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCag                                                      <  2:1224130/101‑1 (MQ=255)


                                             gATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCagg                                                     >  1:402742/1‑100 (MQ=255)


                                             gATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCCCCCCGGCCCACCCAAAGAGCGCGGCGGccgag                                                     >  1:1537461/1‑98 (MQ=255)


                                              aTCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCCGCGCACCGGCACAGCCAGAGAGCGCGGGGGCCagac                                                    >  2:3636881/1‑100 (MQ=255)


                                               tCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagc                                                   >  1:605271/1‑101 (MQ=255)


                                                cGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagcc                                                  >  2:523468/1‑101 (MQ=255)


                                                 ggCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagcca                                                 <  1:3037944/101‑1 (MQ=255)


                                                 ggCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagcca                                                 <  1:3927734/101‑1 (MQ=255)


                                                     aGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCCGCGCACCGGCCCCGCCAGAGAGCGCGGCGGCCCGAGCCCGccc                                             >  1:1146125/1‑101 (MQ=255)


                                                     aGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCCGCGCACCGGCACCGCCAGAGAGCGCGGCGGCCAGAGCCAGccc                                             >  2:2899157/1‑101 (MQ=255)


                                                     aGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCCGCCCACCGCCACCGCCCGAGAGCGCGGCGGCCAGGGCCGGccc                                             >  2:323116/1‑101 (MQ=255)


                                                      gCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGcccc                                            <  2:1185472/101‑1 (MQ=255)


                                                      gCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGcccc                                            >  1:131122/1‑101 (MQ=255)


                                                       cGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCCCAGCCAGAGAGCGCGGCGGCCAGAGCCCGCCCCg                                           >  2:2365220/1‑101 (MQ=255)


                                                       cGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCg                                           >  1:4124581/1‑101 (MQ=255)


                                                        ggCATGTCGATCGCGGCGGTCGAC.GCGCGCGGGC.TTGGCC.CCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCga                                          <  1:2963822/101‑1 (MQ=255)


                                                        ggCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCgc                                          >  1:822662/1‑100 (MQ=255)


                                                        ggCATGGCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCga                                          <  2:3050789/101‑1 (MQ=255)


                                                         gCATGGCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCgag                                         <  2:1905022/101‑1 (MQ=255)


                                                           aTGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGagag                                       <  1:1945390/101‑1 (MQ=255)


                                                            tGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAgg                                      >  1:2532374/1‑101 (MQ=255)


                                                             gTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAggg                                     >  1:4192747/1‑101 (MQ=255)


                                                              tCGATCGCGGGGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGc                                    <  1:432565/101‑1 (MQ=255)


                                                              tCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGc                                    <  1:3204688/101‑1 (MQ=255)


                                                              gcGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCC.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGc                                    <  1:3384694/100‑1 (MQ=255)


                                                               cGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCCGCCCCCCGGCCCAGCCAGGAGGGGCGGGGGCCCGACCCAGcccccggggggcg                                   >  2:4119593/1‑92 (MQ=255)


                                                                gATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCCGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAAAGCCAGCCCCGACAGGGCAg                                  >  2:2504583/1‑101 (MQ=255)


                                                                gATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.A.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGt                                 >  2:3696534/1‑101 (MQ=255)


                                                                   cgcgGCGGTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCa                               >  2:3551113/1‑101 (MQ=255)


                                                                     cggctgGCGAC.GCGCGCNAGC.TTGGCA.CCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAg                             <  2:2059258/101‑1 (MQ=255)


                                                                     cggcggTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGGGCCCCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCCGCCCCGAGCCGGCAAGCAAg                             >  1:416384/1‑101 (MQ=255)


                                                                     cggcggTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAg                             >  2:249680/1‑101 (MQ=255)


                                                                        cggTCGAC.GCGCGCGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGACAGAGAGCGCGGCGGCCAAAGTCCACGCCGAGCGGGCCGACAAGGac                          >  2:938870/1‑100 (MQ=255)


                                                                         ggTCGAC.GCGCGCGAGC.TTGGCA.CCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAc                         <  2:1118118/101‑1 (MQ=255)


                                                                               acgcgcgcGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAcggcgg                    <  1:2080021/97‑1 (MQ=255)


                                                                                 gcgcgcGAGC.TTGGCA.CCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGc                  <  1:692803/99‑1 (MQ=255)


                                                                                 gcgcgcGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGc                  >  1:2216829/3‑101 (MQ=255)


                                                                                  cgcgcGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCggggc                 >  2:648718/2‑99 (MQ=255)


                                                                                   gcgcGAGC.TTGGCA.CCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGccc                <  1:935583/101‑1 (MQ=255)


                                                                                   gcgcGAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGccc                <  2:3139210/101‑1 (MQ=255)


                                                                                    cgcgAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGcacc               >  2:1512613/1‑98 (MQ=255)


                                                                                     gcgAGC.TTGGCA.CCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGa              <  1:2498740/101‑1 (MQ=255)


                                                                                     gcgAGC.TTGGCA.CCT.C.ACCCCCCCCCGGCGCCCCGGCCAAGCCCGAGAGCGCGGCGGCCAGAGCCAGCCCCGCGAGGGCAGCCAAGGAACGGCGGGgcccg              >  2:3702780/1‑99 (MQ=255)


                                                                                      cgAGC.TTGGCC.CCT.C.CCCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGaa             <  2:2920884/101‑1 (MQ=255)


                                                                                         agcttGGCA.CCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGAAgc           <  1:3839445/98‑1 (MQ=255)


                                                                                         agcttGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGAAgc           >  1:3826743/4‑101 (MQ=255)


                                                                                          gcttGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGGCCGGAgcg          >  2:391212/3‑101 (MQ=255)


                                                                                          gcttGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCCAAgcg          >  1:184853/3‑101 (MQ=255)


                                                                                            ttGGCA.CCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGAAGCGCa        <  2:3285470/101‑1 (MQ=255)


                                                                                            ttGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGGCCGAAGCGCa        >  2:2325513/1‑101 (MQ=255)


                                                                                            ttGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGCAGCGCa        >  1:3404283/1‑101 (MQ=255)


                                                                                             tGGCA.CCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGAAGCGCaa       <  2:605981/101‑1 (MQ=255)


                                                                                             tGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGGCCgcaagaacg       >  2:1619065/1‑93 (MQ=255)


                                                                                             tGGCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGGCCCAAacgcca       >  2:2730923/1‑99 (MQ=255)


                                                                                              ggCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGGCCGAAGGGCCAg      >  2:80229/1‑101 (MQ=255)


                                                                                              ggCA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGAAGCGCAAg      <  2:332005/101‑1 (MQ=255)


                                                                                              gccA.CCT.C.A.CCCCCCGCGGCGCACCGGCACAGCCAGAGAGGGCGGCGGCCAGAGCCAGCCGCGAGAGGGCAGTCAAGGAACGGCGGGCCCGAAGCGCAAgg     <  1:4230988/99‑1 (MQ=255)


                                                                                                cA.CCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGGCCGGAACGCaaagg    >  1:495091/1‑98 (MQ=255)


GCAGCGCCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.G.GCGCGAGCTTTGGCA.CCT.C.A.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGAAGCGCAAGGCGC  >  NC_014034/1745354‑1745549

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 22 ≤


ATCG

< 31 ≤


ATCG

< 40 ≤


ATCG