Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,769,370 +G 91.5% coding (442/744 nt) RCAP_rcc01636 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,769,3701.→G91.5% 25.6 ‑2.3 ‑0.2118coding (442/744 nt)RCAP_rcc01636hypothetical protein
Reads supporting (aligned to +/- strand):  new base (53/55):  ref base (2/8):  total (55/63)
Fisher's exact test strand distribution p-value = 1.02e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.96e-01

Only 100 of 133 total aligned reads displayed.

TGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTC.GGCA.GGG.GCGGTGGT.C.CC.CC.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGTGCCGATGCCG  >  NC_014034/1769272‑1769462
                                                                                                       |                                                                                              
 gtgCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.cg                                                                                                <  1:1245670/101‑2 (MQ=255)
  tgCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.cgc                                                                                               <  1:3299590/101‑3 (MQ=255)
    cGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.cgcgc                                                                                             <  2:2967897/101‑5 (MQ=255)
       cAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.cgcgcgcg                                                                                          <  1:2784587/101‑8 (MQ=255)
          cGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.cgcgcgcgggc                                                                                       >  1:1144200/1‑91 (MQ=255)
             cGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCttt                                                                                  <  2:2202098/101‑1 (MQ=255)
             cGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCttt                                                                                  <  1:3037034/101‑1 (MQ=255)
              ggCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTg                                                                                 <  1:1982205/101‑1 (MQ=255)
                 gATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGGCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GGGGTGGG.CGCG.CG.CGGGCTTTGcgt                                                                              >  2:1217587/1‑99 (MQ=255)
                   tCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CTGGCTTTGCCTTg                                                                            <  1:2299382/101‑1 (MQ=255)
                   gcAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTg                                                                            <  2:1572157/100‑1 (MQ=255)
                    cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGTAG.CG.CG.CGGGCTTTGCCTTgg                                                                           >  2:2821239/1‑101 (MQ=255)
                    cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTgg                                                                           >  2:235642/1‑101 (MQ=255)
                    cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTgg                                                                           <  1:2233351/101‑1 (MQ=255)
                    cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTgg                                                                           >  2:1899126/1‑101 (MQ=255)
                    cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTgg                                                                           <  2:2643979/101‑1 (MQ=255)
                     aGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.C.GCGCG.CGGGCTTTGCCTTggg                                                                          <  1:670479/101‑1 (MQ=255)
                       gACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGt                                                                        <  2:3975372/101‑1 (MQ=255)
                       gACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGt                                                                        <  1:2883474/101‑1 (MQ=255)
                       gACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGt                                                                        <  2:2136656/101‑1 (MQ=255)
                        aCCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGGG.CG.CGGGCTTTGCCTTGGGGTc                                                                       >  2:1937473/1‑101 (MQ=255)
                        aCCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTc                                                                       >  2:1450826/1‑101 (MQ=255)
                         ccAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCg                                                                      <  2:2937211/101‑1 (MQ=255)
                         ccAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCg                                                                      >  2:2378010/1‑101 (MQ=255)
                         ccAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.C.GCGCG.CGGGCTTTGCCTTGGGGTCg                                                                      <  1:4079433/101‑1 (MQ=255)
                         ccAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGGGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCg                                                                      >  2:1817786/1‑101 (MQ=255)
                          cAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGa                                                                     <  2:3327859/101‑1 (MQ=255)
                           aaCTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAg                                                                    <  2:778175/101‑1 (MQ=255)
                           aaCTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAg                                                                    >  1:347767/1‑101 (MQ=255)
                             cTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgc                                                                  >  1:3423895/1‑101 (MQ=255)
                              tGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcg                                                                 >  2:1208065/1‑101 (MQ=255)
                               ggATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcgc                                                                >  2:46243/1‑101 (MQ=255)
                               ggATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcgc                                                                >  1:3969553/1‑101 (MQ=255)
                                gATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCt                                                               <  2:3177130/101‑1 (MQ=255)
                                gATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCt                                                               >  1:1562735/1‑101 (MQ=255)
                                gATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCt                                                               <  2:2164029/101‑1 (MQ=255)
                                 aTGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCtt                                                              <  2:3276502/101‑1 (MQ=255)
                                 aTGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCtt                                                              <  1:468210/101‑1 (MQ=255)
                                 aTGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTAGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCtt                                                              <  1:3641139/101‑1 (MQ=255)
                                  tGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTc                                                             <  1:1510527/101‑1 (MQ=255)
                                   gCGGCCCGCGCGGCGGAAATCGTCGCGGGTTTGGGCGCGGTCGATGTCGGGCA.GGGTGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTcc                                                            <  1:4216750/101‑1 (MQ=255)
                                   gCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTcc                                                            <  1:2523619/101‑1 (MQ=255)
                                   gCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTcc                                                            <  2:2989991/101‑1 (MQ=255)
                                   gCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.C.GCGCG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTcc                                                            <  1:1068924/101‑1 (MQ=255)
                                    cGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.C.GCGCG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCg                                                           <  1:1079022/101‑1 (MQ=255)
                                     ggCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCgg                                                          >  1:3278945/1‑101 (MQ=255)
                                     ggCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCgg                                                          >  2:3593301/1‑101 (MQ=255)
                                        ccGGGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.C.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCa                                                       <  2:676384/101‑1 (MQ=255)
                                        ccGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.C.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCa                                                       <  2:2605153/101‑1 (MQ=255)
                                          gcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAcc                                                     >  1:765803/1‑101 (MQ=255)
                                             cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                  <  2:2998556/101‑1 (MQ=255)
                                             cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTCCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                  <  2:823470/101‑1 (MQ=255)
                                              ggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATg                                                 <  1:3814694/101‑1 (MQ=255)
                                                gggAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgcg                                               <  1:3422116/100‑1 (MQ=255)
                                                  gAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCt                                             >  2:295746/1‑101 (MQ=255)
                                                  gAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCt                                             >  2:3905805/1‑101 (MQ=255)
                                                     aTCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCt                                          >  2:3529993/1‑101 (MQ=255)
                                                     aTCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCt                                          <  2:557516/101‑1 (MQ=255)
                                                     aTCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCt                                          <  2:3423650/101‑1 (MQ=255)
                                                       cgtcgCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTg                                        <  1:3785511/101‑1 (MQ=255)
                                                       cgtcgCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTg                                        >  1:3480116/1‑101 (MQ=255)
                                                       cgtcgCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTg                                        >  1:1476272/1‑101 (MQ=255)
                                                        gtcgCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGa                                       >  1:3893975/1‑101 (MQ=255)
                                                        gtcgCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGa                                       >  2:3743757/1‑101 (MQ=255)
                                                        gtcgCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGa                                       <  1:2316397/101‑1 (MQ=255)
                                                          cgcgGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                                     >  1:1395543/1‑101 (MQ=255)
                                                               cTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtc                                >  1:329194/1‑101 (MQ=255)
                                                               cTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgcg                                >  2:4134382/1‑99 (MQ=255)
                                                                tttGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtcg                               >  2:3822110/1‑101 (MQ=255)
                                                                  tGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgcc                             >  2:3082358/1‑101 (MQ=255)
                                                                  tGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgcc                             <  2:2615049/101‑1 (MQ=255)
                                                                  tGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgcc                             >  1:3419872/1‑101 (MQ=255)
                                                                     gcgcGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCcgcg                          >  1:2568216/1‑101 (MQ=255)
                                                                       gcgGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCa                        >  1:2143524/1‑101 (MQ=255)
                                                                        cgGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCAt                       <  1:2094907/101‑1 (MQ=255)
                                                                        cgGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCAt                       <  2:1097712/101‑1 (MQ=255)
                                                                         ggTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATc                      <  1:3002802/101‑1 (MQ=255)
                                                                         ggTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATc                      <  2:1422703/101‑1 (MQ=255)
                                                                          gTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcg                     >  1:1003139/1‑101 (MQ=255)
                                                                          gTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcg                     >  1:3714907/1‑101 (MQ=255)
                                                                           tCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgc                    >  2:1151696/1‑101 (MQ=255)
                                                                           tCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgc                    >  1:82102/1‑101 (MQ=255)
                                                                           tCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgc                    >  1:719352/1‑101 (MQ=255)
                                                                           tCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCAATGCGCTCCTTGATTTCGTCGCCGCGCATcgc                    >  2:816486/1‑101 (MQ=255)
                                                                            cGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgcg                   >  1:1392039/1‑101 (MQ=255)
                                                                            cGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgcg                   >  1:2453065/1‑101 (MQ=255)
                                                                            cGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgcg                   <  1:2918290/101‑1 (MQ=255)
                                                                            cGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgcg                   <  2:4044011/101‑1 (MQ=255)
                                                                               tgtcgggcagggg.gCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGc                >  2:2367170/11‑101 (MQ=255)
                                                                                   gggcagggg.gCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGTCCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGt            >  1:3630997/7‑101 (MQ=255)
                                                                                      cagggg.gCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgcc         <  2:318402/98‑1 (MQ=255)
                                                                                      cagggg.gCGGTGGT.C.GCGCG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgcc         >  2:3867327/4‑101 (MQ=255)
                                                                                       agggg.gCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccg        <  1:3593554/99‑1 (MQ=255)
                                                                                       agggg.gCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccg        <  1:432052/99‑1 (MQ=255)
                                                                                         ggg.gCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgat      <  1:2807747/101‑1 (MQ=255)
                                                                                         ggg.gCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgat      <  2:1050907/101‑1 (MQ=255)
                                                                                         ggg.gCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgat      <  2:3235914/101‑1 (MQ=255)
                                                                                          gg.gCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatg     <  2:3893716/101‑1 (MQ=255)
                                                                                             gCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgcc   <  2:33990/101‑1 (MQ=255)
                                                                                              cGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgccg  <  1:3518630/101‑1 (MQ=255)
                                                                                                       |                                                                                              
TGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTC.GGCA.GGG.GCGGTGGT.C.CC.CC.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGTGCCGATGCCG  >  NC_014034/1769272‑1769462

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG