BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,823,740	+C	100%	intergenic (+25/‑148)	RCAP_rcc01687 → / → RCAP_rcc01688	HK97 family phage major capsid protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,823,740	1	.→C	100.0%	30.9	144	intergenic (+25/‑148)	RCAP_rcc01687/RCAP_rcc01688	HK97 family phage major capsid protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (83/49): ref base (0/12): total (83/61)
Fisher's exact test strand distribution p-value = 1.68e-05
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.13e-01

Only 100 of 174 total aligned reads displayed.


CTATGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.A.C.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCTGCGCC  >  NC_014034/1823640‑1823835


  aTGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.A.c.c                                                                                                <  2:1124261/101‑1 (MQ=255)


    gCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.A.c.ccc                                                                                              <  1:740546/101‑2 (MQ=255)


     cGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.A.c.cccg                                                                                             <  1:1446239/101‑3 (MQ=255)


     cGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.A.c.cccg                                                                                             <  2:2791943/101‑3 (MQ=255)


       aGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.A.c.cccggc                                                                                           <  2:3814887/101‑5 (MQ=255)


        gCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.A.c.cccggct                                                                                          <  1:3813848/101‑6 (MQ=255)


         cAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTg                                                                                         >  2:1778697/1‑101 (MQ=255)


         cAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTg                                                                                         <  2:2747342/101‑1 (MQ=255)


         cAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTg                                                                                         <  2:783694/101‑1 (MQ=255)


           aGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTgcc                                                                                       <  1:4199422/101‑1 (MQ=255)


           aGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTgcc                                                                                       >  2:615220/1‑101 (MQ=255)


            gCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTgccg                                                                                      <  1:312876/101‑1 (MQ=255)


              gggTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTgccgcc                                                                                    <  2:2316645/101‑1 (MQ=255)


               ggTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGccgccg                                                                                   <  1:1772675/101‑1 (MQ=255)


               ggTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGccgccg                                                                                   >  1:3372015/1‑101 (MQ=255)


                gTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCgg                                                                                  <  1:1205891/101‑1 (MQ=255)


                 tGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGt                                                                                 >  2:2098994/1‑101 (MQ=255)


                 tGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGt                                                                                 <  2:2394656/101‑1 (MQ=255)


                  gggCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGtt                                                                                <  1:1100306/101‑1 (MQ=255)


                  gggCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGtt                                                                                >  2:3935889/1‑101 (MQ=255)


                   ggcggcGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTc                                                                               <  2:3979469/101‑1 (MQ=255)


                    gcggcgATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCg                                                                              <  2:2915697/101‑1 (MQ=255)


                      ggcgATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGcc                                                                            >  1:8402/1‑101 (MQ=255)


                      ggcgATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGcc                                                                            >  1:4038873/1‑101 (MQ=255)


                       gcgATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCg                                                                           <  2:4146566/101‑1 (MQ=255)


                        cgATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCgg                                                                          >  2:1804228/1‑101 (MQ=255)


                         gATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGttagatcgg                                                                         >  2:2292821/1‑94 (MQ=255)


                         gATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGt                                                                         >  2:2650471/1‑101 (MQ=255)


                         gATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGt                                                                         <  2:2055071/101‑1 (MQ=255)


                           tGTCAGCGTTTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCGGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTcg                                                                       <  2:1201816/101‑1 (MQ=255)


                           tGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTcg                                                                       >  2:3966398/1‑101 (MQ=255)


                           tGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTcg                                                                       >  2:386121/1‑101 (MQ=255)


                             tCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTcggg                                                                     <  2:143581/101‑1 (MQ=255)


                                gCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTcgggcgg                                                                  >  1:300982/1‑101 (MQ=255)


                                 cGATTTCGCCGCGATTAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGATCGCCGGTcgggcggc                                                                 >  2:1359549/1‑100 (MQ=255)


                                  gATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGGCgggggggc                                                                >  1:3954290/1‑101 (MQ=255)


                                      tCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGgcgggcgcgg                                                            <  1:1670480/101‑1 (MQ=255)


                                       cgccgcGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGgcgggcgcggg                                                           >  1:252372/1‑101 (MQ=255)


                                       cgccgcGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGgcgggcgcggg                                                           >  1:3452587/1‑101 (MQ=255)


                                       cgccgcGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGgcgggcgcggg                                                           <  1:615284/101‑1 (MQ=255)


                                          cgcgATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCtt                                                        <  1:2932400/101‑1 (MQ=255)


                                           gcgATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTc                                                       <  2:1063082/101‑1 (MQ=255)


                                            cgATCAAGCGGTTGAAGTTTGCCGCCTCGTACCGGGCGGTGAAGGGGCCGGGGGCACC.C.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTcc                                                      <  1:652184/101‑1 (MQ=255)


                                             gATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCg                                                     >  2:2975253/1‑101 (MQ=255)


                                               tCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGc                                                   >  2:1211996/1‑101 (MQ=255)


                                                caggCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGTcc                                                  <  1:3130553/98‑1 (MQ=255)


                                                cAAGCTGTTGAAGTTTGCCGCCGCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGcc                                                  <  1:1069327/101‑1 (MQ=255)


                                                 acgCTGTTGAAGTTGGCCACCTCGAATCGGACGGTGAAGGGGCCGGGGGCA.C.CACCGG.CCCCCGCCGGTTCGCCGGTCGGGTGGGGGCGGGCTTCCGGCCg                                                 <  2:682842/99‑1 (MQ=255)


                                                  aGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGc                                                >  1:2607436/1‑101 (MQ=255)


                                                   gCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGAA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCt                                               >  2:3277426/1‑101 (MQ=255)


                                                     tGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTACGGNCGCTGc                                             >  1:2095017/1‑101 (MQ=255)


                                                      gTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGcc                                            >  1:235171/1‑101 (MQ=255)


                                                      gTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGcc                                            >  2:2879634/1‑101 (MQ=255)


                                                       ttGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCt                                           <  2:967196/101‑1 (MQ=255)


                                                       ttGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCt                                           <  1:3421061/101‑1 (MQ=255)


                                                       ttGAAGTTTGCCGCCGCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCt                                           <  2:1962240/101‑1 (MQ=255)


                                                        tGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTa                                          <  1:1400359/101‑1 (MQ=255)


                                                        tGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTa                                          <  1:2848862/101‑1 (MQ=255)


                                                         gAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAg                                         >  1:754706/1‑101 (MQ=255)


                                                         gAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAg                                         <  1:2099132/101‑1 (MQ=255)


                                                         gAAGTTGGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAg                                         <  1:625916/101‑1 (MQ=255)


                                                           aGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAgct                                       >  2:1498673/1‑101 (MQ=255)


                                                           aGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAgct                                       >  2:3258107/1‑101 (MQ=255)


                                                            gTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGCCACC.C.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAgctg                                      <  1:2756581/101‑1 (MQ=255)


                                                             tttGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAgctgc                                     >  2:3692132/1‑101 (MQ=255)


                                                             tttGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAgctgc                                     >  2:2472552/1‑101 (MQ=255)


                                                              ttGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCACC.C.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAgctgct                                    <  1:1778816/101‑1 (MQ=255)


                                                              ttGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAgctgct                                    >  1:2247438/1‑101 (MQ=255)


                                                               tGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctc                                   >  1:3294027/1‑101 (MQ=255)


                                                                  cgccTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctccct                                >  2:3421385/1‑101 (MQ=255)


                                                                   gccTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctccctc                               >  2:1765588/1‑101 (MQ=255)


                                                                    tcTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctccctcc                              <  2:3922411/100‑1 (MQ=255)


                                                                    ccTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctccctcc                              >  2:1838238/1‑101 (MQ=255)


                                                                     cgcGTAACGGGCGGTGAGGGGGCCGGGGGCACC.C.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccg                             <  2:2937407/99‑1 (MQ=255)


                                                                     cTCGTAACGGGCGGTGAAGGGGCCGGGGGAA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccg                             >  1:3864151/1‑101 (MQ=255)


                                                                      tCGTAGCGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccgt                            <  2:2512847/101‑1 (MQ=255)


                                                                      tCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccgt                            >  1:2513031/1‑101 (MQ=255)


                                                                      tCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccgt                            >  1:3810657/1‑101 (MQ=255)


                                                                       cGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctccctccaga                           >  1:3922671/1‑98 (MQ=255)


                                                                       cGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTTCCtccgtc                           >  2:4128753/1‑101 (MQ=255)


                                                                        gTAGCGGGCGGTGAAGGGGCCGGGGGCACC.C.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccgtcc                          <  2:1651233/101‑1 (MQ=255)


                                                                         tAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccgtccg                         >  2:2566556/1‑101 (MQ=255)


                                                                         tAACGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccgtccg                         >  1:1601701/1‑101 (MQ=255)


                                                                          agcGGGGGGTGAGGGGGNGGGGGGCACC.C.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGa                        <  2:745985/99‑1 (MQ=255)


                                                                          aaCGGGCGGTGAAGGGGCCGGGGGCACC.C.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGa                        <  2:3466802/101‑1 (MQ=255)


                                                                          aaCGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGa                        >  1:2619297/1‑101 (MQ=255)


                                                                          aaCGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGa                        >  2:2613426/1‑101 (MQ=255)


                                                                            cGGGCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGt                      >  1:1940673/1‑101 (MQ=255)


                                                                             gggCGGTGAAGGGGCGGGGGGCACC.C.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTg                     <  1:3682021/101‑1 (MQ=255)


                                                                             gggCGGTGAAGGGGCCGAGGGCA.ACC.CCGG.CTGCCGCCGGTTCGGCGGTCGGACGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTg                     >  2:3516701/1‑101 (MQ=255)


                                                                              ggCGGTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTgg                    >  2:4029382/1‑101 (MQ=255)


                                                                                 ggTGAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCgg                 >  2:2918381/1‑101 (MQ=255)


                                                                                   tagaGGGGCGGGGGGCA.A.C.CCGGCCTTCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTg               <  2:300937/98‑1 (MQ=255)


                                                                                    gAAGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGc              >  2:251361/1‑101 (MQ=255)


                                                                                      aGGGGCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCgg            >  2:1280431/1‑101 (MQ=255)


                                                                                        gggCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCgggg          >  2:503934/1‑101 (MQ=255)


                                                                                         ggCCGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCggggg         >  1:784302/1‑100 (MQ=255)


                                                                                          gCCGGGGGCACC.C.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTc        <  2:564377/101‑1 (MQ=255)


                                                                                            cGGGGGCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCTg      <  1:1830992/101‑1 (MQ=255)


                                                                                                ggCA.ACC.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCTGCGcc  >  2:2001719/1‑101 (MQ=255)


CTATGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.A.C.CCGG.CTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCTGCGCC  >  NC_014034/1823640‑1823835

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 23 ≤


ATCG

< 32 ≤


ATCG

< 40 ≤


ATCG