Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,843,420 +C 100% intergenic (+82/+66) ppiB → / ← tyrS peptidyl‑prolyl cis‑trans isomerase B/tyrosyl‑tRNA synthetase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,843,4201.→C100.0% 492.4 155intergenic (+82/+66)ppiB/tyrSpeptidyl‑prolyl cis‑trans isomerase B/tyrosyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  new base (94/61):  ref base (0/0):  total (94/61)

Only 100 of 167 total aligned reads displayed.

AAGGCCACGATCGAGTGACACGGGCGCA.GGGGGCTCTGCCCCCCCTCTG.TGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCG.TCCGCT.GGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.GGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCG  >  NC_014034/1843321‑1843512
                                                                                                      |                                                                                                
 aGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgc                                                                                                  <  1:2714826/101‑2 (MQ=25)
  gtccACGATCGAGTGACACGTGCGCATGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCCCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgct                                                                                                 <  1:1018393/99‑3 (MQ=21)
    ccACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgctcc                                                                                               <  1:1434091/101‑5 (MQ=25)
    ccACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgctcc                                                                                               <  1:2001518/101‑5 (MQ=25)
      aCGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGc                                                                                             <  2:2415826/101‑1 (MQ=25)
           cGAGTGACACGGGCGCAGGGGGGCTCTGCCCCCCC.CTGCTGCGCATTCAACCCCCGGGATATTTTCGCCAAGGTGAAATCGAAAAGGGGGCTCCGCT.gggg                                                                                       >  2:3224611/1‑101 (MQ=21)
           cGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gggg                                                                                       <  2:3267290/101‑1 (MQ=25)
             aGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gggggc                                                                                     <  2:1613083/101‑3 (MQ=21)
                gACACGGGCGCAGGGGGGCTCTG.CTCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gggggcgcc                                                                                  >  2:3796306/1‑96 (MQ=21)
                   ccGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCCCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCtt                                                                                <  2:862563/100‑1 (MQ=18)
                    ggggCGCAGGGGGGCTCTG.CCCCCGTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCttt                                                                               <  2:2395717/100‑1 (MQ=18)
                    cGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCttt                                                                               <  2:3729234/101‑1 (MQ=21)
                    cGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCttt                                                                               <  2:186366/101‑1 (MQ=21)
                     gggCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTc                                                                              <  1:1659703/101‑1 (MQ=21)
                      ggCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCa                                                                             <  1:2626057/101‑1 (MQ=21)
                               gggCTCTG.CCCCACTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCcac                                                                    <  1:952929/101‑1 (MQ=25)
                                ggCTCTG.CCCCCCTCTGCGGCGCCATCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCcaca                                                                   <  1:1651313/101‑1 (MQ=21)
                                  ctctG.CCCCCCTCTGCTGCGCATTCCCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACaaa                                                                 <  1:1541924/101‑1 (MQ=25)
                                  ctctG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACaaa                                                                 <  2:2165508/101‑1 (MQ=25)
                                  ctctG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACaaa                                                                 <  1:2583253/101‑1 (MQ=25)
                                    tctgccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAat                                                                <  1:2300666/97‑1 (MQ=25)
                                      tgccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAatat                                                              >  1:2926876/3‑101 (MQ=25)
                                      tgccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAatat                                                              >  2:3480434/3‑101 (MQ=25)
                                       gccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATc                                                             >  2:1297926/2‑101 (MQ=37)
                                        ccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATcc                                                            >  1:750541/1‑101 (MQ=37)
                                          ccccTCTGCTGCGCATTCGCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCg                                                          <  2:1579864/101‑1 (MQ=37)
                                          ccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCg                                                          <  2:3847621/101‑1 (MQ=37)
                                           cccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCgg                                                         <  1:1272987/101‑1 (MQ=37)
                                            ccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCggg                                                        >  2:3543625/1‑101 (MQ=37)
                                              tctgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGGGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGt                                                      >  1:459343/6‑101 (MQ=37)
                                               ctgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.c                                                    >  2:1557932/5‑101 (MQ=38)
                                                tgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.c.c                                                  >  1:2301436/4‑101 (MQ=38)
                                                tgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.c.c                                                  >  1:2650634/4‑101 (MQ=38)
                                                tgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.c.c                                                  >  1:2154128/4‑101 (MQ=38)
                                                 gctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.g                                                <  2:1464289/99‑1 (MQ=38)
                                                 gctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAAGGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.g                                                >  2:1384171/3‑101 (MQ=38)
                                                   tgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.ggg                                              >  2:518096/1‑101 (MQ=38)
                                                   tgCGCATTCACCCCCCGGGATATTTTCGCCAAGATGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.ggg                                              <  1:3665135/101‑1 (MQ=38)
                                                    gcgcATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.gggg                                             <  2:1570599/101‑2 (MQ=38)
                                                    gcgcATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.gggg                                             >  1:3589076/1‑100 (MQ=38)
                                                     cgcATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.ggggc                                            <  2:1640987/101‑3 (MQ=38)
                                                     cgcATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.ggggc                                            >  2:644148/1‑99 (MQ=38)
                                                      gcATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.ggggca                                           <  1:2220411/101‑4 (MQ=38)
                                                       cATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.ggggcag                                          >  1:2115244/1‑97 (MQ=37)
                                                        aTTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.ggggcaga                                         >  1:1855141/1‑96 (MQ=37)
                                                          tCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGc                                        <  1:3560932/101‑1 (MQ=37)
                                                          tCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGc                                        >  1:2066169/1‑101 (MQ=37)
                                                          tCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGc                                        >  2:4020936/1‑101 (MQ=37)
                                                          tCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGc                                        >  1:789030/1‑101 (MQ=37)
                                                           cACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGcc                                       >  1:92500/1‑101 (MQ=25)
                                                            aCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGccc                                      >  1:372553/1‑101 (MQ=25)
                                                             ttccgatctatatTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGcccc                                     <  1:2179615/92‑1 (MQ=21)
                                                             ccccccGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGcccc                                     <  2:2866482/101‑1 (MQ=25)
                                                             ccccccGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGcccc                                     >  1:453316/1‑101 (MQ=25)
                                                              cctccGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCg                                    <  1:2593534/98‑1 (MQ=25)
                                                              cccccGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCg                                    >  2:1160218/1‑101 (MQ=25)
                                                                cccGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGc                                  >  2:3542951/1‑101 (MQ=25)
                                                                 ccGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGcc                                 >  1:4018442/1‑101 (MQ=25)
                                                                   gggATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCtt                               >  1:2425260/1‑101 (MQ=25)
                                                                    ggATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTg                              <  2:2107623/101‑1 (MQ=25)
                                                                     gATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGc                             >  2:2905744/1‑101 (MQ=25)
                                                                     gATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGc                             >  1:388078/1‑101 (MQ=25)
                                                                      atatTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGccccagatcggaa                            >  2:2179470/1‑92 (MQ=21)
                                                                      atatTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCt                            >  2:2531256/1‑101 (MQ=25)
                                                                      atatTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCt                            >  1:626858/1‑101 (MQ=25)
                                                                      atatTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCt                            >  1:375937/1‑101 (MQ=25)
                                                                       tatTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTc                           >  1:2940042/1‑101 (MQ=25)
                                                                        atTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCa                          >  2:3284649/1‑101 (MQ=25)
                                                                        atTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCa                          >  1:846193/1‑101 (MQ=25)
                                                                        atTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCa                          <  1:2221327/101‑1 (MQ=25)
                                                                        atTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCa                          >  1:3350336/1‑101 (MQ=25)
                                                                         ttttCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCAg                         >  2:605647/1‑101 (MQ=25)
                                                                         ttttCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCAg                         <  1:3423030/101‑1 (MQ=25)
                                                                         ttttCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCAg                         >  2:3158437/1‑101 (MQ=25)
                                                                         ttttCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCAg                         >  2:3687523/1‑101 (MQ=25)
                                                                          tttCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCAGc                        >  1:1330139/1‑101 (MQ=25)
                                                                            tCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGGTCAGccc                      >  2:589737/1‑101 (MQ=25)
                                                                              gCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCAGCCCag                    <  1:3106036/101‑1 (MQ=25)
                                                                              gCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCAGCCCag                    <  1:644225/101‑1 (MQ=25)
                                                                               ccAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCAGCCCaga                   <  2:3739124/101‑1 (MQ=25)
                                                                               ccAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCAGCCCaga                   >  1:2978098/1‑101 (MQ=25)
                                                                                cAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCAGCCCagag                  >  1:1966523/1‑101 (MQ=25)
                                                                                 aaGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CTG.GGGCAGAGCCCCGGCCTTGCTCAGCCCAgaga                 >  1:3609002/1‑101 (MQ=25)
                                                                                 aaGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCAGCCCAgaga                 >  2:3107405/1‑101 (MQ=25)
                                                                                  aGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGac                >  1:2849687/1‑101 (MQ=25)
                                                                                   ggTGAAATGGAAAAGGGCGCTGCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGaca               >  1:3119627/1‑101 (MQ=25)
                                                                                    gTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGacac              >  2:4038810/1‑101 (MQ=25)
                                                                                      gAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCa            >  1:552938/1‑101 (MQ=25)
                                                                                      gAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCa            >  2:2863153/1‑101 (MQ=25)
                                                                                       aaaTGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAg           >  1:4003259/1‑101 (MQ=25)
                                                                                       aaaTGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAg           >  1:1632287/1‑101 (MQ=25)
                                                                                       aaaTGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAg           >  1:2249161/1‑101 (MQ=25)
                                                                                        aaTGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAgc          >  2:3899124/1‑101 (MQ=25)
                                                                                         aTGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGCTGC.G.GGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAgcg         >  2:4026141/1‑101 (MQ=21)
                                                                                           ggAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCa       >  1:628241/1‑101 (MQ=25)
                                                                                             aaaaGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATg     <  2:1010242/101‑1 (MQ=25)
                                                                                              aaaGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATgg    >  1:3224665/1‑101 (MQ=25)
                                                                                               aaGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGgc   <  2:2022965/101‑1 (MQ=25)
                                                                                               aaGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGgc   >  2:1483816/1‑101 (MQ=25)
                                                                                                aGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGG.C.CGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACCCCAGCGCATGgcg  >  1:419710/1‑101 (MQ=25)
                                                                                                      |                                                                                                
AAGGCCACGATCGAGTGACACGGGCGCA.GGGGGCTCTGCCCCCCCTCTG.TGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCG.TCCGCT.GGGGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.GGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCG  >  NC_014034/1843321‑1843512

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG