BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,867,833	+C	100%	intergenic (‑235/+80)	RCAP_rcc01729 ← / ← RCAP_rcc01730	pyridine nucleotide‑disulfide oxidoreductase/U32 family peptidase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,867,833	1	.→C	100.0%	31.0	95	intergenic (‑235/+80)	RCAP_rcc01729/RCAP_rcc01730	pyridine nucleotide‑disulfide oxidoreductase/U32 family peptidase
Reads supporting (aligned to +/- strand): new base (39/45): ref base (0/11): total (39/56)
Fisher's exact test strand distribution p-value = 2.38e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.94e-01

Only 100 of 168 total aligned reads displayed.


GAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CG.CCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCAA  >  NC_014034/1867733‑1867928


 aaaaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCCGCGGCGCG.AA.A.a.cg.c                                                                                                      >  2:216517/1‑101 (MQ=255)


 aaaaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGACCCGGCGGCGCG.AA.A.g.cg.c                                                                                                      <  1:1437567/101‑1 (MQ=255)


 aaaaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGAGCCCAGCCCCGGCGGCGCG.AA.A.g.cg.c                                                                                                      >  1:2978151/1‑101 (MQ=255)


  aaaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.cg.cc                                                                                                     <  2:1869371/101‑1 (MQ=255)


   aaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.cg.ccc                                                                                                    <  2:1157199/101‑2 (MQ=255)


   aaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.cg.ccc                                                                                                    <  1:2632202/101‑2 (MQ=255)


   aaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.cg.ccc                                                                                                    <  2:3889778/101‑2 (MQ=255)


   aaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.cg.ccc                                                                                                    <  2:3714508/101‑2 (MQ=255)


    aGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.cg.cccg                                                                                                   <  1:2702841/101‑3 (MQ=255)


    aGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.cg.cccg                                                                                                   <  2:1810522/101‑3 (MQ=255)


    aGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACCCCCAGCCCCCGCGGCGCG.AA.A.G.cg.cccg                                                                                                   >  2:255846/1‑99 (MQ=255)


     gCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCCGCCCCGGCGGCGCG.AA.A.G.cg.cccgc                                                                                                  >  1:3688487/1‑98 (MQ=255)


      cACTGCTTTCCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.cg.cccgcc                                                                                                 <  2:831215/101‑5 (MQ=255)


       aCTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCTGCCCCCGGGGCGGG.AA.C.G.Cg.cccccca                                                                                                >  1:2317450/1‑99 (MQ=37)


         tGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCATCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.Tg                                                                                              <  2:574882/101‑1 (MQ=255)


         tGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCCGCGGCGCG.AA.A.A.Cg.ccccccccg                                                                                              >  1:1018648/1‑97 (MQ=37)


          gCTTTCCCCCCCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.Tgg                                                                                             <  1:86401/101‑1 (MQ=25)


           cTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCCGCCCCCGCGGCGCG.CA.A.G.CGCCCGCCC.Tgg.g                                                                                           >  2:3968928/1‑101 (MQ=21)


                ccccGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCCGCCCCCGCGGCGCG.AA.A.G.CGCCCGCCC.Cgg.gcgggg                                                                                      >  1:1912385/1‑101 (MQ=21)


                ccccGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCC.Cgg.gcgggg                                                                                      >  1:2109484/1‑101 (MQ=25)


                 cccgcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.Tgg.gcggggc                                                                                     <  2:2351479/101‑1 (MQ=255)


                   cgcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGg.gcggggcgg                                                                                   <  1:4002087/101‑1 (MQ=255)


                    gcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAAAGCCCAGCCCCGGCGGCGCG.AA.A.A.CGCCCGCCC.TGG.gcgggggggg                                                                                  >  1:3062902/1‑101 (MQ=21)


                     cccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGgggcgggc                                                                                 <  2:257585/101‑1 (MQ=255)


                     cccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCG.AA.A.A.CGCCCCCCA.CGG.gcggggcgggg                                                                                 >  2:2906585/1‑100 (MQ=21)


                        gtgtCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCC.TCG.GCGGGGCGGgggcg                                                                              >  1:3818840/1‑100 (MQ=21)


                         tgtCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.g                                                                           <  2:3363284/101‑1 (MQ=255)


                            cgggACGGCTTTGCAAAGACCGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGGGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.cc                                                                       <  2:4158911/98‑1 (MQ=17)


                             gcgACAGCGTTGCAAAGCCCGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCg                                                                      <  2:1354999/101‑1 (MQ=18)


                             gcgACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCg                                                                      <  2:240820/101‑1 (MQ=255)


                             gcgACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCG.AA.A.G.CGCCCCCCA.CCG.gccgggcggggggcggcgc                                                                         >  2:3300665/1‑92 (MQ=18)


                              cgACAGCGTTGCAAAGACCGCACCCCCAGGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGa                                                                     <  1:1006688/101‑1 (MQ=18)


                               gACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCG.AA.A.G.CGCCCGCCA.CGG.GCGGGGCGGGGGG.C.GG.CCGA.g                                                                   >  2:3577064/1‑101 (MQ=18)


                                cttccgatcaaaaGCCCGCACCCCCAAGCGCGCGAGAGCCCAGCGCCCAGCCCCGGCGGCGCT.AA.ACC.CC.CCGCCA.TGG.GCGGGTCGGGCGC.T.GG.CCGA.gg                                                                  <  1:3151849/91‑1 (MQ=17)


                                aCAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGCGCG.AA.A.C.CGCCCGCCA.TGG.GCGGGGCGGGGGC.C.GG.CCGA.gg                                                                  >  1:3837214/1‑101 (MQ=18)


                                aCAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCG.AA.A.A.CGCCCGCCC.CGG.GCGGGGGGGGCGC.C.gggccggg                                                                    >  1:3276002/1‑95 (MQ=255)


                                 cggCGTGGCAAAGCCCGCACCCCCAAGCGCGCGAGAGGCCAGCCCCCAGCCCCGGCGGCGCA.AA.A.GCCC.CCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.ggg                                                                 <  1:17375/99‑1 (MQ=17)


                                 cAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.ggg                                                                 <  1:1353604/101‑1 (MQ=255)


                                  aGCTTTGCAAAGCCCGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.GCCC.CCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGc                                                                <  1:1754856/101‑1 (MQ=17)


                                    ctgcggcaggACCGCATCCCCAAGCACGGCAGAGGCCAGCGTCCCGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCC.TGG.GCGGGGCGGGCGC.C.GG.GCGG.GGGccc                                                              >  1:3165836/10‑100 (MQ=18)


                                     gTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGCGCG.AA.A.A.CGCCCGCCC.TGG.GCGGGGGGGGGGC.T.GG.GCGG.ggggcct                                                             >  2:1040257/1‑97 (MQ=17)


                                     gTTGCAAAGACCGCACCCCCACGCGCGCGAGAGGCCAGCCCCCAGCCCCGGCGGCGCG.AA.A.GCCC.CCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCtt                                                             <  1:216555/101‑1 (MQ=17)


                                       tGCAAAGCCCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTg                                                           <  2:3427096/101‑1 (MQ=255)


                                         aaaaGCCCGCACCCCCAAGCGGGCGAGAGGCCGGCCCCCACCCCGGGGGGCGCA.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTggg                                                         <  1:1046165/100‑1 (MQ=14)


                                          caaGATCGCCTCCCCAAGCGCGCGGGAGGCCTGCGTCCCGCCCCGGCGGCGCG.AA.T.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.A.GG.GCGC.AGGccccgtggc                                                        >  2:1379149/2‑94 (MQ=18)


                                           aaGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGGGC.G.GG.CCGA.GGGGCCTTggggg                                                       >  1:62018/1‑100 (MQ=17)


                                           aaGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCACCCCCCGCCGCGCC.AA.A.G.GGCCCGCCA.CTG.GCCGGGCGGGGGC.G.CG.CCGA.GGGGCCTTGGGGc                                                       >  2:4002450/1‑101 (MQ=9)


                                            aGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGGGC.TGGG.CGGG.GGGCGTTTGGGGc                                                       >  2:3911557/1‑101 (MQ=17)


                                            aGACCGCATCCCCAAGCGCGCGAGAGGCCAGAGCCCAGCCCCCGGGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.G.GG.CCGAGGGGCCTTTGGGGc                                                       >  1:729778/1‑101 (MQ=17)


                                             gcccccacccccAAGCGCGCGAGAGGCCAGCCCCCAGCCCCGGCGGCGCG.AA.A.GCCC.CCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGccc                                                     <  1:3398735/93‑1 (MQ=17)


                                             gcccGCACCCCCAAGCGCGCGAGAGGCCAGCCCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGccc                                                     <  1:3851453/99‑1 (MQ=17)


                                             gACCGTTTCCCCAAGCACGACAGAGGCCTGCGGCCAGCCCCCGCGGCGCG.AA.A.G.CGCCCCCCG.AGG.GCGGGGGGGGGGCGC.GG.CCGC.GGGcgctgtggccc                                                      >  2:1524055/1‑91 (MQ=9)


                                             gACCGCCCCCCGACGCCCGCGAGGGGCCGGCCCCCAGCCCCGGCGGCGCG.AACG.G.CC.CCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGccc                                                     <  2:1956588/101‑1 (MQ=14)


                                             gACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCCGCCCCGGCGGCGCG.GA.A.G.CGCCCCCCC.CGG.GGGGGGCGGGCGC.C.CG.GGCA.GGGCCGTTGGGGacc                                                     >  2:3564755/1‑101 (MQ=2)


                                             gACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCCGCCCCCGCGGCGCG.AA.A.G.CGACCGCCC.TGG.GCGGGGGGGGCGC.C.GGCCCGG.GGGCCTTGGGGGcc                                                      >  2:1158823/1‑101 (MQ=14)


                                             cgccGCACCCCCAGGCGCGCGAGAGGCCAGCCCCCAGCCCCGGCGGCGCG.AA.A.GCCC.CCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGccc                                                     <  1:4057428/99‑1 (MQ=17)


                                              aCCGCCGCCCCAGGCGCGGGAGAGCCCAGCGCCCGGCCCGGGCGGCGCA.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGGGC.T.GG.CCGA.GGGCCTTTGGGGccac                                                    <  2:372538/101‑3 (MQ=14)


                                                cGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCCGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGGGGGCGC.C.GG.GCGG.GGGGCGTTTGGGCCCCcg                                                  >  2:723393/1‑101 (MQ=14)


                                                cGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.C.GG.CCGA.GGGCCCTTGGGGgccccc                                                  >  1:2097080/1‑100 (MQ=17)


                                                cGCACCCCCAAGCGCGCGAGGGGCCGCCGCCCGCCCCCGGGGGCGCG.AA.A.GCCC.CCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCcg                                                  <  1:3143799/101‑1 (MQ=17)


                                                   accccaAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCcggcg                                               <  2:2447225/99‑1 (MQ=255)


                                                    tCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.A.CGCCCGCCA.TGG.GCGGGGGGGGGGC.C.GG.GCGAGGGGCCTTTGGGG.CCCcggcgg                                              >  1:881462/1‑101 (MQ=14)


                                                      cccgggggcgcgAGGGGCCGCCCCCGAGCCCGGGCGGCGCG.AA.A.GCCC.CCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTc                                            <  1:3000359/94‑1 (MQ=14)


                                                      cccAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTc                                            <  2:2849889/101‑1 (MQ=255)


                                                       ccAGGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCa                                           <  2:2306678/101‑1 (MQ=255)


                                                       ccAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGGGC.T.GG.CCCA.GGGCCTTTGGGGTCCCcggcgggcc                                           >  1:1394085/1‑98 (MQ=17)


                                                         aaGCGCGCGGGGGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAgg                                         <  1:1850919/101‑1 (MQ=17)


                                                          cgtgacggaggGGCCAG.GCCCCGGCCCGGGGGGGCGAAA.A.G.CGCCCGCCAGTGGCGCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCTCGGCGGTctt                                          <  2:3623400/94‑3 (MQ=17)


                                                           gcgcgcGAGGGCCCGCCCCCCACCCCCGGCGGCGCG.AA.A.GCCC.CCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAgggg                                       <  1:2915706/101‑1 (MQ=14)


                                                                cgAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.GCGA.GGGCCCTTGGGGCCCCCGGCGGGCAggggggtgt                                  >  1:998820/1‑97 (MQ=14)


                                                                 gagaGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGGCTTTGGGGGCCCCGGGGGGCCGGGGGGTCTg                                 >  2:228066/1‑101 (MQ=14)


                                                                 gagaGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTTGGGGCCCCCGCGGTCCGGGGGGTCTg                                 >  2:2479085/1‑101 (MQ=14)


                                                                 gagaGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGGCCCCGGCGGGCAGGGGGGTCTg                                 >  1:2224066/1‑101 (MQ=17)


                                                                 gagaGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCCA.GGGCCCTTGGGGCCCCCGGCGGGCAGGGGGGTCTg                                 >  1:577162/1‑101 (MQ=14)


                                                                  agagGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGAGGGGCCTTTGGGGCCCCC.GCGGGCAGGGGGGGCTGt                                >  2:3885097/1‑101 (MQ=14)


                                                                   ggggCCGGCGCCCGGCCCCGGCGGCGCA.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGtt                               <  1:4097389/99‑1 (MQ=17)


                                                                   gacgCCACCCCCCAGCCCCGGCGGCGCA.AA.G.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGtt                               <  2:2734898/98‑1 (MQ=17)


                                                                    agGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGGTCTGTTg                              >  1:3730715/1‑101 (MQ=255)


                                                                    agGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTg                              >  2:3510447/1‑101 (MQ=255)


                                                                      gCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGGGCTGTTgcg                            >  2:3124890/1‑101 (MQ=17)


                                                                       ccAGCGCCCAGCCCGGGGGGCGCG.AA.A.GCCC.CCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTgcgc                           <  2:1938265/101‑1 (MQ=18)


                                                                       ccAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTgcgc                           >  1:1902883/1‑101 (MQ=255)


                                                                        cAGCCCCCAGCCCCCGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCa                          <  2:2819367/101‑1 (MQ=18)


                                                                         aGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGGCAGGGGGATCTGTTGCGCAt                         >  2:3846657/1‑101 (MQ=255)


                                                                           cgcCCGGCCCCGGGGGCGCG.AA.A.GCCC.CCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATgg                       <  2:2045975/101‑1 (MQ=18)


                                                                           cgcCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.C.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGGTCTGTTGCGCATgg                       >  1:1144575/1‑101 (MQ=17)


                                                                            gcCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGc                      >  2:551531/1‑101 (MQ=255)


                                                                            gcCCAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGGCAGGGGGGTCTGTTGCGCATGGc                      >  2:886925/1‑101 (MQ=18)


                                                                            ccccAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGc                      <  1:1165910/100‑1 (MQ=255)


                                                                             cccAGCCCGGGGGGGGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGcc                     <  1:1588163/101‑1 (MQ=18)


                                                                              ccAGCCCCGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCcg                    >  2:1729035/1‑101 (MQ=255)


                                                                               ccgCCCGGGCGGCGCA.AA.C.GCCC.CCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCcgc                   <  1:3562877/99‑1 (MQ=17)


                                                                                 ccccgGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGt                 <  2:917268/100‑1 (MQ=255)


                                                                                   ccggggggcgGG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCa               <  1:2479269/95‑1 (MQ=18)


                                                                                   cccGGCGGCGCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCa               >  2:3202037/1‑101 (MQ=255)


                                                                                        cggcgCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGcgcc          >  1:4232097/1‑101 (MQ=255)


                                                                                         ggcgCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGcgccg         >  2:3412450/1‑101 (MQ=255)


                                                                                         ggcgCG.AA.A.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGCTGCGCAGTGCCGGGTCAGcgccc         >  2:996783/1‑100 (MQ=18)


                                                                                                aa.a.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCa   >  1:2341050/1‑101 (MQ=255)


                                                                                                 a.a.G.CGCCCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCaa  >  1:2635580/1‑101 (MQ=255)


GAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCG.AA.A.G.CG.CCGCCA.TGG.GCGGGGCGGGCGC.T.GG.CCGA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCAA  >  NC_014034/1867733‑1867928

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 22 ≤


ATCG

< 32 ≤


ATCG

< 40 ≤


ATCG