Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,948,851 +G 90.0% coding (1069/2472 nt) quiP ← acyl‑homoserine‑lactone acylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,948,8511.→G90.0% 65.6 0.0 ‑0.5219coding (1069/2472 nt)quiPacyl‑homoserine‑lactone acylase
Reads supporting (aligned to +/- strand):  new base (80/117):  ref base (9/13):  total (89/130)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.08e-01

Only 100 of 231 total aligned reads displayed.

TGATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.GCCC.ACCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGC  >  NC_014034/1948751‑1948943
                                                                                                     |                                                                                              
 gATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.g                                                                                               >  1:3549509/1‑101 (MQ=255)
     cGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Ggccc                                                                                           >  2:2132536/1‑100 (MQ=255)
      gACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Ggccca                                                                                          >  1:3319710/1‑99 (MQ=255)
          gcagcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Ggcccaaccc                                                                                      <  2:1419237/101‑7 (MQ=255)
          gcagcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCAGCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Ggcccaaccc                                                                                      <  2:418464/101‑7 (MQ=255)
           cagcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Ggcccaaccct                                                                                     <  2:2377801/101‑8 (MQ=255)
            agcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Ggcccaaccctc                                                                                    >  1:2581584/1‑93 (MQ=255)
            agcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Ggcccaaccctc                                                                                    >  2:2462170/1‑93 (MQ=255)
             gcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCAGGTGGTGAACTCGGCCCAACCCTCg                                                                                    >  2:1160257/1‑101 (MQ=255)
               aCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCggg                                                                                  >  2:3600615/1‑101 (MQ=255)
                 cGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGc                                                                                <  2:432524/101‑1 (MQ=255)
                  gggCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCCCCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCt                                                                               <  1:2818742/101‑1 (MQ=255)
                  gggCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCt                                                                               >  2:1452723/1‑101 (MQ=255)
                  gggCCGTTTGCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCt                                                                               <  2:3649932/101‑1 (MQ=255)
                   ggCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTg                                                                              >  2:2028639/1‑101 (MQ=255)
                   ggCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTg                                                                              >  2:3232979/1‑101 (MQ=255)
                    gCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGc                                                                             >  2:2768541/1‑101 (MQ=255)
                      cGTTTTCGGTCCAGAGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCgg                                                                           >  2:1839370/1‑101 (MQ=255)
                       gTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGt                                                                          >  1:1085659/1‑101 (MQ=255)
                        ttttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTa                                                                         >  2:2232723/1‑101 (MQ=255)
                        ttttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTa                                                                         >  2:2918356/1‑101 (MQ=255)
                        ttttCGGTCCAGCGCAGGGGGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTa                                                                         >  1:2089947/1‑101 (MQ=255)
                         tttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTag                                                                        >  1:847762/1‑100 (MQ=255)
                         tttCGGTCCAGCGCAGGGTGAGGGGGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGtcg                                                                        >  2:1584901/1‑99 (MQ=255)
                          ttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTagc                                                                       <  2:3063624/101‑3 (MQ=255)
                          ttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTagc                                                                       <  1:1430727/101‑3 (MQ=255)
                           tCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTagcg                                                                      >  2:3535934/1‑98 (MQ=255)
                           tCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTagcg                                                                      >  2:3833969/1‑98 (MQ=255)
                             ggTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTagcgct                                                                    >  2:3737210/1‑96 (MQ=255)
                             ggTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGGGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTagcgcc                                                                    >  1:1902747/1‑96 (MQ=255)
                              gTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTc                                                                   <  2:2340641/101‑1 (MQ=255)
                              gTCCAGCGCAGGGTGAGGGTGATCGGCGCGCCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTc                                                                   <  1:266559/101‑1 (MQ=255)
                                 cAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCggg                                                                >  1:3106814/1‑101 (MQ=255)
                                  aGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGc                                                               <  1:1448510/101‑1 (MQ=255)
                                  aGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGc                                                               <  1:566646/101‑1 (MQ=255)
                                    cgcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGt                                                             <  1:1866582/101‑1 (MQ=255)
                                    cgcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGt                                                             >  1:580552/1‑101 (MQ=255)
                                     gcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGtt                                                            <  2:1531691/101‑1 (MQ=255)
                                      cAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGttt                                                           >  1:2268553/1‑101 (MQ=255)
                                      cAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGttt                                                           >  1:3697491/1‑101 (MQ=255)
                                      cAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGttt                                                           <  2:1869721/101‑1 (MQ=255)
                                        gggtgagggtgaTCGGCGCGGCATCCTTGAGCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTc                                                         <  2:693690/101‑1 (MQ=255)
                                        gggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTc                                                         <  2:2153755/101‑1 (MQ=255)
                                        gggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTc                                                         <  1:2311289/101‑1 (MQ=255)
                                           tgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGa                                                      >  1:44770/1‑101 (MQ=255)
                                            gagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGAt                                                     >  2:3972019/1‑101 (MQ=255)
                                            gagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGAt                                                     <  1:888891/101‑1 (MQ=255)
                                             agggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATc                                                    <  1:3982258/101‑1 (MQ=255)
                                               ggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCaa                                                  <  1:3667904/101‑1 (MQ=255)
                                                gtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAg                                                 <  1:3874755/101‑1 (MQ=255)
                                                  gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGtt                                               <  2:1322607/101‑1 (MQ=255)
                                                  gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGtt                                               <  1:1556332/101‑1 (MQ=255)
                                                  gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGtt                                               >  1:978601/1‑101 (MQ=255)
                                                  gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGtt                                               >  2:3509829/1‑101 (MQ=255)
                                                   aTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTc                                              <  2:1741809/101‑1 (MQ=255)
                                                     cGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCt                                            <  2:2581393/101‑1 (MQ=255)
                                                     cGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCt                                            <  1:1287187/101‑1 (MQ=255)
                                                       gcgcGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcg                                          <  1:4133944/101‑1 (MQ=255)
                                                          cgGCATCCTTGGCCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgacg                                       <  1:3295346/101‑1 (MQ=255)
                                                           ggCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgacga                                      >  2:1777868/1‑101 (MQ=255)
                                                            gCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGaa                                     >  2:3097497/1‑101 (MQ=255)
                                                            gCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGaa                                     <  2:659651/101‑1 (MQ=255)
                                                            gCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGaa                                     >  1:1801764/1‑101 (MQ=255)
                                                               tCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                  >  2:853226/1‑101 (MQ=255)
                                                                 cTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGCTCAAGTTCCTCGACGAACAggc                                >  2:564488/1‑100 (MQ=255)
                                                                 cTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGt                                >  2:3305237/1‑101 (MQ=255)
                                                                 cTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGt                                <  2:3717081/101‑1 (MQ=255)
                                                                  ttGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTc                               >  2:1076745/1‑101 (MQ=255)
                                                                  ttGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTc                               >  2:3216675/1‑101 (MQ=255)
                                                                    gACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCt                             >  1:3928356/1‑101 (MQ=255)
                                                                    ctccGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCt                             <  2:1590325/99‑1 (MQ=255)
                                                                     aCCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTc                            <  1:1746728/101‑1 (MQ=255)
                                                                     aCCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTc                            <  2:167486/101‑1 (MQ=255)
                                                                     aCCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTc                            <  2:2727499/101‑1 (MQ=255)
                                                                      ccGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCtag                           >  1:1590434/1‑99 (MQ=255)
                                                                       cGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGt                          >  1:4148420/1‑101 (MQ=255)
                                                                         gatctaTCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCa                        <  2:1922024/96‑1 (MQ=255)
                                                                          gACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                       <  1:3421149/101‑1 (MQ=255)
                                                                           aCGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATc                      <  2:3390205/101‑1 (MQ=255)
                                                                            cGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCg                     <  2:79442/101‑1 (MQ=255)
                                                                             gATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCgg                    <  1:2864410/101‑1 (MQ=255)
                                                                             gATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCgg                    <  1:2619946/101‑1 (MQ=255)
                                                                             gATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCgc                    >  1:3203798/1‑100 (MQ=255)
                                                                              aTCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCaagatc                   >  1:1922150/1‑96 (MQ=255)
                                                                              aTCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGc                   <  1:4163095/101‑1 (MQ=255)
                                                                              aTCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGc                   <  1:802861/101‑1 (MQ=255)
                                                                                cACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGa                 >  2:767713/1‑101 (MQ=255)
                                                                                 aCCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGTCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGaa                <  1:1656844/101‑1 (MQ=255)
                                                                                  ccTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAg               <  2:2181979/101‑1 (MQ=255)
                                                                                  ccTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAg               <  2:2102868/101‑1 (MQ=255)
                                                                                    ttGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggc             <  2:1236591/101‑1 (MQ=255)
                                                                                    ttGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggc             <  1:2432571/101‑1 (MQ=255)
                                                                                    ttGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggc             <  1:1753264/101‑1 (MQ=255)
                                                                                     tGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcg            <  1:3233218/101‑1 (MQ=255)
                                                                                      gCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcgg           <  2:479545/101‑1 (MQ=255)
                                                                                      gCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcgg           <  2:1769444/101‑1 (MQ=255)
                                                                                       cGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcggc          >  1:3593719/1‑101 (MQ=255)
                                                                                        gggTGGTGAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGgcggcg         <  2:4148158/101‑1 (MQ=255)
                                                                                             gtgAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGAt    <  1:2918575/101‑1 (MQ=255)
                                                                                              tgAACTCGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATg   >  2:3067480/1‑101 (MQ=255)
                                                                                                     |                                                                                              
TGATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.GCCC.ACCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGC  >  NC_014034/1948751‑1948943

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG