Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,948,855 +A 100% coding (1065/2472 nt) quiP ← acyl‑homoserine‑lactone acylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,948,8551.→A100.0% 750.7 197coding (1065/2472 nt)quiPacyl‑homoserine‑lactone acylase
Reads supporting (aligned to +/- strand):  new base (79/117):  ref base (0/1):  total (79/118)

Only 100 of 223 total aligned reads displayed.

CCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.GCCC.A.CCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGC  >  NC_014034/1948755‑1948943
                                                                                                       |                                                                                          
 cGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.Ggccc                                                                                            >  2:2132536/1‑100 (MQ=255)
  gACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.Ggccca                                                                                           >  1:3319710/1‑99 (MQ=255)
  gACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.Ggccca                                                                                           <  1:1640515/101‑3 (MQ=255)
     ggCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.Ggcccaacc                                                                                        <  2:1269622/101‑6 (MQ=255)
      gcagcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.Ggcccaaccc                                                                                       <  2:1419237/101‑7 (MQ=255)
       cagccCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCCGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.Ggcccaaccct                                                                                      <  2:3462177/101‑8 (MQ=255)
        agcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.Ggcccaaccctc                                                                                     >  1:2581584/1‑93 (MQ=255)
        agcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.Ggcccaaccctc                                                                                     >  2:2462170/1‑93 (MQ=255)
         gcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCAGGTGGTGAACT.CGGCCCAA.CCCTCg                                                                                    >  2:1160257/1‑101 (MQ=255)
             cGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGc                                                                                <  2:432524/101‑1 (MQ=255)
              gggCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCCCCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCt                                                                               <  1:2818742/101‑1 (MQ=255)
              gggCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCt                                                                               >  2:1452723/1‑101 (MQ=255)
              gggCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCt                                                                               <  2:2257099/101‑1 (MQ=255)
               ggCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTg                                                                              >  2:2028639/1‑101 (MQ=255)
               ggCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTg                                                                              >  2:3232979/1‑101 (MQ=255)
                gCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGc                                                                             >  2:2768541/1‑101 (MQ=255)
                gCCGTTGTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCACCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGc                                                                             <  2:1805869/101‑1 (MQ=255)
                 ccGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCg                                                                            <  1:2435121/101‑1 (MQ=255)
                 ccGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCg                                                                            >  1:2650280/1‑101 (MQ=255)
                 ccGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCg                                                                            <  2:3636713/101‑1 (MQ=255)
                  cGTTTTCGGTCCAGAGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCgg                                                                           >  2:1839370/1‑101 (MQ=255)
                   gTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGt                                                                          >  1:1085659/1‑101 (MQ=255)
                   gTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGt                                                                          >  1:1835737/1‑101 (MQ=255)
                    ttttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTa                                                                         <  1:2950780/101‑1 (MQ=255)
                    ttttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTa                                                                         >  2:2232723/1‑101 (MQ=255)
                    ttttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGAGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTa                                                                         >  1:3669428/1‑101 (MQ=255)
                     tttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTag                                                                        >  2:152619/1‑100 (MQ=255)
                     tttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTag                                                                        >  1:847762/1‑100 (MQ=255)
                     tttCGGTCCAGCGCAGGGTGAGGGGGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGtcg                                                                        >  2:1584901/1‑99 (MQ=255)
                      ttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTagc                                                                       <  2:3063624/101‑3 (MQ=255)
                       tCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTagcg                                                                      >  2:3833969/1‑98 (MQ=255)
                       tCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTagcg                                                                      >  2:3535934/1‑98 (MQ=255)
                         ggTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGGGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTagcgcc                                                                    >  1:1902747/1‑96 (MQ=255)
                           tCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCg                                                                  >  1:914048/1‑101 (MQ=255)
                             cAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCggg                                                                >  1:3106814/1‑101 (MQ=255)
                                cgcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGt                                                             <  1:1866582/101‑1 (MQ=255)
                                 gcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCCCCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtt                                                            <  1:573437/101‑1 (MQ=255)
                                  cAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGttt                                                           <  2:1869721/101‑1 (MQ=255)
                                  cAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGttt                                                           >  1:2268553/1‑101 (MQ=255)
                                    gggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTc                                                         <  1:2311289/101‑1 (MQ=255)
                                       tgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGa                                                      >  1:44770/1‑101 (MQ=255)
                                        gagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGAt                                                     >  2:3972019/1‑101 (MQ=255)
                                           ggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCaa                                                  <  1:4073779/101‑1 (MQ=255)
                                            gtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAg                                                 <  1:3874755/101‑1 (MQ=255)
                                            gtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAg                                                 <  2:1776263/101‑1 (MQ=255)
                                             tgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGCAGCGCTCGGGCGTTTTCGGATCAAGt                                                >  2:2009248/1‑101 (MQ=255)
                                              gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGtt                                               <  1:1556332/101‑1 (MQ=255)
                                              gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGtt                                               <  2:1322607/101‑1 (MQ=255)
                                               aTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTc                                              <  2:1741809/101‑1 (MQ=255)
                                                tCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTcc                                             >  1:3717335/1‑101 (MQ=255)
                                                 cGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCt                                            <  2:3800859/101‑1 (MQ=255)
                                                 cGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCt                                            <  2:2581393/101‑1 (MQ=255)
                                                   gcgcGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTGG.GCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcg                                          <  1:3559653/101‑1 (MQ=255)
                                                   gcgcGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcg                                          <  1:4133944/101‑1 (MQ=255)
                                                        gCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGaa                                     >  2:3097497/1‑101 (MQ=255)
                                                        gCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGaa                                     <  2:659651/101‑1 (MQ=255)
                                                         cATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAAc                                    >  2:4162833/1‑101 (MQ=255)
                                                         cATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAAc                                    <  1:978720/101‑1 (MQ=255)
                                                         cATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAAc                                    <  2:3353655/101‑1 (MQ=255)
                                                           tCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                  >  2:853226/1‑101 (MQ=255)
                                                           tCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                  <  1:3287325/101‑1 (MQ=255)
                                                           tCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                  <  1:2757635/101‑1 (MQ=255)
                                                             cTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGCTCAAGTTCCTCGACGAACAggc                                >  2:564488/1‑100 (MQ=255)
                                                             cTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGt                                <  2:3717081/101‑1 (MQ=255)
                                                             cTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGt                                >  2:3305237/1‑101 (MQ=255)
                                                              ttGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTc                               >  2:1076745/1‑101 (MQ=255)
                                                              ttGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTc                               <  1:1673593/101‑1 (MQ=255)
                                                                gACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCt                             >  1:3928356/1‑101 (MQ=255)
                                                                ctccGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCt                             <  2:1590325/99‑1 (MQ=255)
                                                                 aCCGTGCCGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.GCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCGCGACGAACAGGTCCTc                            <  1:675051/101‑1 (MQ=255)
                                                                 aCCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTc                            <  2:2727499/101‑1 (MQ=255)
                                                                 aCCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTc                            <  1:1746728/101‑1 (MQ=255)
                                                                 aCCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTc                            <  2:167486/101‑1 (MQ=255)
                                                                 aCCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTc                            <  2:2145275/101‑1 (MQ=255)
                                                                   cGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGt                          >  1:4148420/1‑101 (MQ=255)
                                                                      gACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                       <  2:2357301/101‑1 (MQ=255)
                                                                      gACGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                       <  1:3421149/101‑1 (MQ=255)
                                                                        cGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCg                     <  2:79442/101‑1 (MQ=255)
                                                                        cGATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCg                     <  1:1452825/101‑1 (MQ=255)
                                                                         gATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCgg                    <  1:2864410/101‑1 (MQ=255)
                                                                         gATCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCgc                    >  1:3203798/1‑100 (MQ=255)
                                                                          aTCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCaagatc                   >  1:1922150/1‑96 (MQ=255)
                                                                          aTCACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGc                   <  1:802861/101‑1 (MQ=255)
                                                                            cACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGa                 >  2:767713/1‑101 (MQ=255)
                                                                            cACCTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGa                 >  1:4189252/1‑101 (MQ=255)
                                                                              ccTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAg               <  2:2102868/101‑1 (MQ=255)
                                                                              ccTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAg               <  2:2181979/101‑1 (MQ=255)
                                                                               cTTGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAgg              <  2:707796/101‑1 (MQ=255)
                                                                                ttGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggc             <  1:1753264/101‑1 (MQ=255)
                                                                                 tGCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcg            <  1:3233218/101‑1 (MQ=255)
                                                                                  gCGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcgg           <  2:479545/101‑1 (MQ=255)
                                                                                   cGGGTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcggc          >  1:3593719/1‑101 (MQ=255)
                                                                                    gggTGGTGAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGgcggcg         <  2:4148158/101‑1 (MQ=255)
                                                                                     ggtggtGAACT.CGGCCC.AGCCCTCGGGGCTGGGGTAGCGCTCGGGCGGTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGcggcgg        <  1:1407643/101‑1 (MQ=255)
                                                                                      gtggtgAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGt       <  2:3593466/101‑1 (MQ=255)
                                                                                        ggtgAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTgc     >  2:2985253/1‑100 (MQ=255)
                                                                                        ggtgAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGa     >  1:2037524/1‑101 (MQ=255)
                                                                                         gtgAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGAt    <  2:3203559/101‑1 (MQ=255)
                                                                                          tgAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATg   >  2:3067480/1‑101 (MQ=255)
                                                                                           gAACT.CGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGGGATGc  >  1:1440649/1‑101 (MQ=255)
                                                                                                       |                                                                                          
CCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.GCCC.A.CCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGC  >  NC_014034/1948755‑1948943

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG