Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,948,873 +G 100% coding (1047/2472 nt) quiP ← acyl‑homoserine‑lactone acylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,948,8731.→G100.0% 746.9 196coding (1047/2472 nt)quiPacyl‑homoserine‑lactone acylase
Reads supporting (aligned to +/- strand):  new base (75/120):  ref base (0/1):  total (75/121)

Only 100 of 220 total aligned reads displayed.

TTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.GCCC.A.CCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTGCGC  >  NC_014034/1948775‑1948966
                                                                                                      |                                                                                             
ttttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTa                                                                                                <  1:2950780/101‑1 (MQ=255)
ttttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTa                                                                                                >  2:2918356/1‑101 (MQ=255)
ttttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTa                                                                                                <  1:4148140/101‑1 (MQ=255)
ttttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGAGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTa                                                                                                >  1:3669428/1‑101 (MQ=255)
ttttCGGTCCAGCGCAGGGGGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTa                                                                                                >  1:2089947/1‑101 (MQ=255)
ttttCGGTCCAGCGCAGAGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTa                                                                                                <  2:692567/101‑1 (MQ=255)
 tttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTag                                                                                               >  2:152619/1‑100 (MQ=255)
 tttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTag                                                                                               >  1:847762/1‑100 (MQ=255)
   tCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTagcg                                                                                             >  2:3535934/1‑98 (MQ=255)
   tCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTagcg                                                                                             >  2:3833969/1‑98 (MQ=255)
   tCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTagcg                                                                                             >  2:476082/1‑98 (MQ=255)
     ggTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTagcgct                                                                                           >  2:3737210/1‑96 (MQ=255)
     ggTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGGGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTagcgcc                                                                                           >  1:1902747/1‑96 (MQ=255)
      gTCCAGCGCAGGGTGAGGGTGATCGGCGCGCCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTc                                                                                          <  1:266559/101‑1 (MQ=255)
       tCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCg                                                                                         >  2:2233767/1‑101 (MQ=255)
         cAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCggg                                                                                       >  1:3106814/1‑101 (MQ=255)
          aGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGc                                                                                      <  2:2431569/101‑1 (MQ=255)
            cgcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGt                                                                                    >  1:580552/1‑101 (MQ=255)
            cgcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGt                                                                                    <  1:1866582/101‑1 (MQ=255)
             gcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtt                                                                                   >  2:1656729/1‑101 (MQ=255)
               agggtgagggtgATCGGCGCGGCATCCTTGACCATGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                                                 <  1:3628637/101‑1 (MQ=255)
                    gagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGAt                                                                            <  1:888891/101‑1 (MQ=255)
                      gggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCa                                                                          <  2:1907050/101‑1 (MQ=255)
                      gggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCa                                                                          >  1:1732487/1‑101 (MQ=255)
                      gggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCa                                                                          <  1:3904114/101‑1 (MQ=255)
                        gtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAg                                                                        >  2:3220952/1‑101 (MQ=255)
                         tgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGCAGCGCTCGGGCGTTTTCGGATCAAGt                                                                       >  2:2009248/1‑101 (MQ=255)
                          gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGtt                                                                      >  1:978601/1‑101 (MQ=255)
                          gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGtt                                                                      <  2:1322607/101‑1 (MQ=255)
                          gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGtt                                                                      >  2:3509829/1‑101 (MQ=255)
                            tCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTcc                                                                    >  1:3717335/1‑101 (MQ=255)
                             cGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCt                                                                   <  2:2581393/101‑1 (MQ=255)
                              ggCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTc                                                                  <  2:410459/101‑1 (MQ=255)
                                cgcgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcga                                                                <  2:612644/101‑1 (MQ=255)
                                 gcgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgac                                                               <  1:3368179/101‑1 (MQ=255)
                                  cgGCATCCTTGGCCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgacg                                                              <  1:3295346/101‑1 (MQ=255)
                                  cgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgacg                                                              >  2:1475541/1‑101 (MQ=255)
                                   ggCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgacga                                                             <  2:4157364/101‑1 (MQ=255)
                                   ggCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgacga                                                             >  2:1777868/1‑101 (MQ=255)
                                     cATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAAc                                                           <  2:3353655/101‑1 (MQ=255)
                                       tCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                                         >  2:42400/1‑101 (MQ=255)
                                       tCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                                         >  2:853226/1‑101 (MQ=255)
                                        ccTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAgg                                                        >  1:3265208/1‑101 (MQ=255)
                                        ccTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAgg                                                        <  1:4118109/101‑1 (MQ=255)
                                         cTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGt                                                       >  2:3305237/1‑101 (MQ=255)
                                         cTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGt                                                       <  2:3717081/101‑1 (MQ=255)
                                          ttGACCGTGACGGTCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTc                                                      <  2:2230236/101‑1 (MQ=255)
                                          ttGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTc                                                      >  1:3199434/1‑101 (MQ=255)
                                          ttGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTc                                                      >  2:1076745/1‑101 (MQ=255)
                                           tGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTcc                                                     <  1:3536187/101‑1 (MQ=255)
                                           tGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTcc                                                     >  2:168686/1‑101 (MQ=255)
                                            ctccGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCt                                                    <  2:1590325/99‑1 (MQ=255)
                                             aCCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTc                                                   <  1:1585010/101‑1 (MQ=255)
                                             aCCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTc                                                   <  2:2145275/101‑1 (MQ=255)
                                             aCCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTc                                                   <  1:1780161/101‑1 (MQ=255)
                                             aCCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTc                                                   <  2:2727499/101‑1 (MQ=255)
                                              ccGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACCAACAGGTCCTCg                                                  <  1:2301890/101‑1 (MQ=255)
                                                gTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTc                                                >  2:3063873/1‑101 (MQ=255)
                                                  gACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                                              <  2:1857727/101‑1 (MQ=255)
                                                    cGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCg                                            <  1:1452825/101‑1 (MQ=255)
                                                     gATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCgg                                           <  1:2864410/101‑1 (MQ=255)
                                                      aTCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCaagatc                                          >  1:1922150/1‑96 (MQ=255)
                                                      aTCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGc                                          <  1:802861/101‑1 (MQ=255)
                                                      aTCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGc                                          <  1:4163095/101‑1 (MQ=255)
                                                       tCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCgaga                                         >  2:213109/1‑98 (MQ=255)
                                                        cACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGa                                        >  2:767713/1‑101 (MQ=255)
                                                        cACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGa                                        >  2:550341/1‑101 (MQ=255)
                                                         aCCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGTCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGaa                                       <  1:1656844/101‑1 (MQ=255)
                                                         aCCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGaa                                       <  1:424654/101‑1 (MQ=255)
                                                          ccTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAg                                      <  1:3572038/101‑1 (MQ=255)
                                                           cTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAgg                                     <  2:707796/101‑1 (MQ=255)
                                                            ttGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggc                                    <  1:1753264/101‑1 (MQ=255)
                                                              gCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcgg                                  <  2:1769444/101‑1 (MQ=255)
                                                                gggTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGgcggcg                                >  1:2450570/1‑101 (MQ=255)
                                                                gggTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTAGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGgcggcg                                >  2:2162901/1‑101 (MQ=255)
                                                                 ggtggtGAACTCGGCCC.AGCCCTCGGGGCTGGGGTAGCGCTCGGGCGGTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGcggcgg                               <  1:1407643/101‑1 (MQ=255)
                                                                  gtggtgAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGt                              <  2:3593466/101‑1 (MQ=255)
                                                                    ggtgAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGa                            >  1:2037524/1‑101 (MQ=255)
                                                                     gtgAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGAt                           <  2:3203559/101‑1 (MQ=255)
                                                                      tgAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATg                          <  1:763238/101‑1 (MQ=255)
                                                                       gAACTCGGCCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGGGATGc                         >  1:1440649/1‑101 (MQ=255)
                                                                         actcggcccaa.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGccc                       <  1:1086782/91‑1 (MQ=255)
                                                                            cggcccaa.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGccccgg                    <  2:2760993/94‑3 (MQ=255)
                                                                            cggcccaa.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAg                    <  2:2498125/94‑1 (MQ=255)
                                                                             ggcccaa.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTGGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGc                   >  2:3090283/7‑101 (MQ=255)
                                                                              gcccaa.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGcc                  <  2:2880748/96‑1 (MQ=255)
                                                                              gcccaa.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGcc                  >  2:1708369/6‑101 (MQ=255)
                                                                               cccaa.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCa                 <  1:1217427/97‑1 (MQ=255)
                                                                                  aa.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGc              >  1:483401/2‑101 (MQ=255)
                                                                                  aa.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGc              >  2:4038098/2‑101 (MQ=255)
                                                                                     cccTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTg            <  2:3106584/101‑1 (MQ=255)
                                                                                       cTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGt          >  2:3039799/1‑101 (MQ=255)
                                                                                        tCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTc         <  2:580488/101‑1 (MQ=255)
                                                                                        tCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTc         <  2:796042/101‑1 (MQ=255)
                                                                                         cGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCg        >  2:1575080/1‑101 (MQ=255)
                                                                                          ggggCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGGGATGACCCAGCCAAGCTGGTCGc       >  1:2982537/1‑101 (MQ=255)
                                                                                           ggtcTGCGGTAGCGCTCGGGGGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAACGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCt      <  1:3972279/98‑1 (MQ=255)
                                                                                           gggCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCt      >  2:793160/1‑101 (MQ=255)
                                                                                             gCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTgc    >  1:2317631/1‑101 (MQ=255)
                                                                                               tGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTgcgc  >  1:2501494/1‑101 (MQ=255)
                                                                                                      |                                                                                             
TTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.GCCC.A.CCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTGCGC  >  NC_014034/1948775‑1948966

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG