Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,044,155 +C 7.2% intergenic (+28/+149) typA → / ← potG1 GTP‑binding protein TypA/BipA/polyamine ABC transporter ATP‑binding protein PotG

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,044,1550.→C 7.2% 37.6 0.0 ‑0.1193intergenic (+28/+149)typA/potG1GTP‑binding protein TypA/BipA/polyamine ABC transporter ATP‑binding protein PotG
Reads supporting (aligned to +/- strand):  new base (6/7):  ref base (81/98):  total (87/106)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.61e-01

Only 100 of 224 total aligned reads displayed.

GCCGAAGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCTTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCG  >  NC_014034/2044055‑2044252
                                                                                                                                                                                                      
gCCGAAGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCt                                                                                                   <  2:3522723/101‑1 (MQ=255)
gCCGAAGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCt                                                                                                   <  1:2982631/101‑1 (MQ=255)
 ccGAAGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCtt                                                                                                  <  1:203766/101‑1 (MQ=255)
    aaGATCGTGCGTCTGCGCAAGCGGCTTCTGGACTCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCttttt                                                                                               <  2:2420721/101‑1 (MQ=255)
    aaGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCttttt                                                                                               <  2:959506/101‑1 (MQ=255)
    aaGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCttttt                                                                                               <  2:109615/101‑1 (MQ=255)
     aGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCtttttc                                                                                              <  1:469400/101‑2 (MQ=255)
     aGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCtttttc                                                                                              <  1:2433524/101‑2 (MQ=255)
     aGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCtttttc                                                                                              >  1:1336863/1‑100 (MQ=255)
       aTCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCtttttcat                                                                                            <  1:4121464/101‑4 (MQ=255)
          gTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCg                                                                                        >  2:875907/1‑101 (MQ=255)
          gTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCg                                                                                        >  2:2556337/1‑101 (MQ=255)
           tGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGt                                                                                       <  1:233515/101‑1 (MQ=255)
            gCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTg                                                                                      >  2:1060252/1‑101 (MQ=255)
             cGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCTTGc                                                                                     >  1:1536160/1‑101 (MQ=255)
             cGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGc                                                                                     <  2:1997216/101‑1 (MQ=255)
               tCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCACAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTTCCg                                                                                   >  1:2205117/1‑101 (MQ=255)
               tCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCg                                                                                   <  2:3044133/101‑1 (MQ=255)
               tCTGCGAAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGGCCGGAAAAAGTGGGAAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCg                                                                                   <  1:3514176/101‑1 (MQ=255)
                  gcgcAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAAt                                                                                <  2:367449/101‑1 (MQ=255)
                  gcgcAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAAt                                                                                <  1:1152725/101‑1 (MQ=255)
                  gcgcAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAAt                                                                                <  2:2275617/101‑1 (MQ=255)
                    gcAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGa                                                                              <  2:3431820/101‑1 (MQ=255)
                     cAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGaa                                                                             <  2:358452/101‑1 (MQ=255)
                     atctcGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGaa                                                                             <  2:3509774/97‑1 (MQ=255)
                          ggCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCa                                                                        >  2:1259042/1‑101 (MQ=255)
                          ggCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCa                                                                        <  2:3747782/101‑1 (MQ=255)
                          ggCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCa                                                                        <  1:1429288/101‑1 (MQ=255)
                          cgCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCa                                                                        <  2:2950108/100‑1 (MQ=255)
                           gCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCAc                                                                       <  2:4166614/101‑1 (MQ=255)
                           gCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCAc                                                                       <  2:1555256/101‑1 (MQ=255)
                            cTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCCTTTTT.ATTCGTGCCGAATGAAATTCaac                                                                      >  2:888219/1‑99 (MQ=255)
                              tCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTc                                                                    <  1:74342/101‑1 (MQ=255)
                              tCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTc                                                                    <  2:3998733/101‑1 (MQ=255)
                               cTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTcc                                                                   <  1:3988103/101‑1 (MQ=255)
                               cTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTcc                                                                   <  2:2196264/101‑1 (MQ=255)
                                tGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTccc                                                                  >  1:2254844/1‑101 (MQ=255)
                                 ggACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTcccc                                                                 >  2:2804831/1‑101 (MQ=255)
                                 ggACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTcccc                                                                 >  2:1887046/1‑101 (MQ=255)
                                 ggACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTcccc                                                                 >  1:507693/1‑101 (MQ=255)
                                    cccGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCg                                                              <  1:3771346/101‑1 (MQ=255)
                                    cccGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCg                                                              <  1:625083/101‑1 (MQ=255)
                                    cccGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCg                                                              >  1:677074/1‑101 (MQ=255)
                                     ccGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGTTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCgg                                                             >  2:3137862/1‑101 (MQ=255)
                                     ccGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCgg                                                             <  2:2737930/101‑1 (MQ=255)
                                       gCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGa                                                           >  2:3885170/1‑101 (MQ=255)
                                       gCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCACCCCGGGa                                                           >  2:3778382/1‑101 (MQ=255)
                                           gAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATAtt                                                       <  1:1193281/101‑1 (MQ=255)
                                           gAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATAtt                                                       >  2:2847828/1‑101 (MQ=255)
                                           gAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGGCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATAtt                                                       <  1:3334264/101‑1 (MQ=255)
                                            aGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATAttt                                                      <  1:2591453/101‑1 (MQ=255)
                                            aGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATAttt                                                      <  2:78440/101‑1 (MQ=255)
                                                cAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAgg                                                  <  2:1713441/101‑1 (MQ=255)
                                                 aaaCGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGc                                                 >  1:594229/1‑101 (MQ=255)
                                                     gcgcCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGa                                             <  1:2866607/101‑1 (MQ=255)
                                                      cgcCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGAt                                            <  2:2314695/101‑1 (MQ=255)
                                                        ccTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATaa                                          <  1:2700306/101‑1 (MQ=255)
                                                         cTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATaaa                                         >  2:3125108/1‑101 (MQ=255)
                                                             cgcTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACt                                     <  2:2913371/101‑1 (MQ=255)
                                                              gcTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACtt                                    <  2:695402/101‑1 (MQ=255)
                                                              gcTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACtt                                    <  2:3058035/101‑1 (MQ=255)
                                                               cTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTg                                   <  2:1797627/101‑1 (MQ=255)
                                                               cTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTg                                   <  1:3676791/101‑1 (MQ=255)
                                                                  ggAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGa                                >  1:310072/1‑101 (MQ=255)
                                                                   gAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAg                               <  2:594165/101‑1 (MQ=255)
                                                                    aaTAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAgg                              >  1:1633228/1‑101 (MQ=255)
                                                                    aaTAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAgg                              >  1:3459138/1‑101 (MQ=255)
                                                                      tAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGgtg                            >  2:4051631/1‑101 (MQ=255)
                                                                       aaGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGgtgt                           >  1:1981866/1‑101 (MQ=255)
                                                                        aGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAgggatt                          >  2:3564091/1‑97 (MQ=255)
                                                                        aGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGtt                          >  1:457165/1‑101 (MQ=255)
                                                                        aGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGtt                          >  2:1672274/1‑101 (MQ=255)
                                                                         gACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCTGTCCTGCCGAATGAAATTCACCTGCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGttc                         <  1:3619756/101‑1 (MQ=38)
                                                                         gACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGttc                         >  2:1852311/1‑101 (MQ=255)
                                                                         gACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGttc                         >  2:3351188/1‑101 (MQ=255)
                                                                          aCGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGttct                        <  2:2863570/101‑1 (MQ=255)
                                                                           cGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGttctt                       <  1:888300/101‑1 (MQ=255)
                                                                              cAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCAt                    >  1:4199145/1‑101 (MQ=255)
                                                                               aaaaGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATc                   >  1:180396/1‑101 (MQ=255)
                                                                               aaaaGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATc                   >  2:1146352/1‑101 (MQ=255)
                                                                                 aaGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCtt                 >  2:1003798/1‑101 (MQ=255)
                                                                                  aGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCttt                <  1:3208939/101‑1 (MQ=255)
                                                                                     ggggCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTg             <  1:1279667/101‑1 (MQ=255)
                                                                                     ggggCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTg             >  1:4065623/1‑101 (MQ=255)
                                                                                      gggCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGc            <  1:227218/101‑1 (MQ=255)
                                                                                      gggCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGc            <  1:1122609/101‑1 (MQ=255)
                                                                                      gggCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGc            <  1:1887166/101‑1 (MQ=255)
                                                                                       ggCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCa           <  2:680841/101‑1 (MQ=255)
                                                                                       ggCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCa           >  1:3818777/1‑101 (MQ=255)
                                                                                         ctctGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCaaa         <  2:1254587/101‑1 (MQ=255)
                                                                                         ctctGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCaaa         <  1:3351433/101‑1 (MQ=255)
                                                                                          tctGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCATCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAg        <  1:2948322/101‑1 (MQ=255)
                                                                                             tgcccccCTTTTTCATTCGTGCCGAATGAAATTCTCCTCCCCCCGGGATATTTGAGGCAAGATAAAGACGTGCGAGGTGTTCTTCATCTTTCTGCAAAGAc      <  1:3823519/99‑1 (MQ=255)
                                                                                             tgcccccCTTTTTCATTCGTGCCGAATGAAATTCGCCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGAc      <  2:1336763/99‑1 (MQ=255)
                                                                                             tgcccccCTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGAc      <  2:1299005/99‑1 (MQ=255)
                                                                                             tgcccccCTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGAc      >  1:1023455/3‑101 (MQ=255)
                                                                                               cccccCTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACcg    <  2:2354540/101‑1 (MQ=255)
                                                                                                ccccCTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACcgc   <  2:261671/101‑1 (MQ=255)
                                                                                                ccccCTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACcgc   <  1:2805050/101‑1 (MQ=255)
                                                                                                 cccCTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACcgcg  >  1:3698247/1‑101 (MQ=255)
                                                                                                                                                                                                      
GCCGAAGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCTTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCG  >  NC_014034/2044055‑2044252

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG