BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,057,825	+C	73.1%	intergenic (‑591/‑311)	RCAP_rcc01900 ← / → RCAP_rcc01901	hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,057,825	1	.→C	73.1%	150.5 ‑1.3 ‑0.1	160	intergenic (‑591/‑311)	RCAP_rcc01900/RCAP_rcc01901	hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein
Reads supporting (aligned to +/- strand): new base (55/62): ref base (25/18): total (80/80)
Fisher's exact test strand distribution p-value = 2.85e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.43e-01

Only 100 of 185 total aligned reads displayed.


AAAATTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.CGCG.CGC.A.GC.G.TT.CCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057725‑2057913


aaaaTTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.t                                                                                                   <  1:1081483/101‑1 (MQ=255)


aaaaTTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.t                                                                                                   <  1:1238535/101‑1 (MQ=255)


aaaaTTGGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.t                                                                                                   <  1:707283/101‑1 (MQ=255)


  aaTTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.c.c                                                                                               >  2:610212/1‑101 (MQ=255)


   aTTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTTGCGACAA.T.c.cc                                                                                              >  2:1423319/1‑100 (MQ=255)


    tttGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.c.ccg                                                                                             >  2:298711/1‑99 (MQ=255)


    tttGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.c.ccg                                                                                             <  2:2733293/101‑3 (MQ=255)


    tttGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.c.ccg                                                                                             <  1:958543/101‑3 (MQ=255)


    tttGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.c.ccg                                                                                             >  1:3098567/1‑99 (MQ=255)


     ttGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.c.ccgc                                                                                            >  1:3231244/1‑98 (MQ=255)


     ttGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.c.ccgc                                                                                            >  1:3323453/1‑98 (MQ=255)


       gTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.c.cg.cgc                                                                                        >  1:2559655/1‑101 (MQ=255)


        tCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.c.cg.cgcc                                                                                       <  2:1895202/101‑2 (MQ=255)


        tCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.c.cg.cgcc                                                                                       >  1:640173/1‑100 (MQ=255)


         ccTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.c.cg.cgccg                                                                                      >  1:1166207/1‑99 (MQ=255)


          cTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.c.cg.cgccga                                                                                     >  2:351669/1‑98 (MQ=255)


          cTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gc                                                                                   >  1:1638798/1‑101 (MQ=255)


          cTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gc                                                                                   >  2:3444635/1‑101 (MQ=255)


          cTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gc                                                                                   >  1:3507570/1‑101 (MQ=255)


           tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.cggcgccgca                                                                                      >  2:2030726/1‑93 (MQ=255)


           tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gca                                                                                  >  2:3617028/1‑100 (MQ=255)


           tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gca                                                                                  >  2:530425/1‑100 (MQ=255)


            gTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaa                                                                                 >  1:2372506/1‑99 (MQ=255)


            gTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaa                                                                                 <  2:2168895/101‑3 (MQ=255)


            gTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaa                                                                                 >  2:2581873/1‑99 (MQ=255)


            gTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaa                                                                                 >  2:2633318/1‑99 (MQ=255)


            gTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaa                                                                                 <  2:1766456/101‑3 (MQ=255)


             tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaag                                                                                >  1:3315584/1‑98 (MQ=255)


             tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaag                                                                                <  1:267659/101‑4 (MQ=255)


              ttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaagc                                                                               >  1:2668288/1‑97 (MQ=255)


              ttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaagc                                                                               >  1:2599195/1‑97 (MQ=255)


               tACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaagcg                                                                              >  1:4138538/1‑96 (MQ=255)


                aCCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaagcgt                                                                             <  1:2957614/101‑7 (MQ=255)


                aCCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaagcgt                                                                             >  1:2021460/1‑95 (MQ=255)


                 ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaagcgtt                                                                            <  2:3021485/101‑8 (MQ=255)


                 ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaagcgtt                                                                            <  1:143419/101‑8 (MQ=255)


                    cccATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaagcgtttcc                                                                         <  1:2090511/101‑11 (MQ=255)


                              cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCAt                                                                 >  1:336033/1‑101 (MQ=255)


                              cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCAt                                                                 >  1:3987653/1‑101 (MQ=255)


                               tGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAC.TAC.CCCGCCGCAA.GCGG.TT.CCGAACCGGCATc                                                                >  2:1560836/1‑101 (MQ=255)


                               tGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATc                                                                <  2:3414405/101‑1 (MQ=255)


                               tGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATc                                                                >  2:366991/1‑101 (MQ=255)


                               tGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGAGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCAta                                                                >  1:1747596/1‑100 (MQ=255)


                                gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCa                                                               >  2:1679883/1‑101 (MQ=255)


                                  ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCatt                                                             <  1:530489/101‑3 (MQ=255)


                                  ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCatt                                                             <  2:3502260/101‑3 (MQ=255)


                                  ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CGCGCGCCGCAA.GC.GTTT.CCGTACCGGCATCatt                                                             >  2:380146/1‑99 (MQ=255)


                                   tCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTc                                                            >  2:3251354/1‑101 (MQ=255)


                                    cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCt                                                           >  1:893358/1‑101 (MQ=255)


                                    cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCt                                                           <  1:2842665/101‑1 (MQ=255)


                                     ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCtt                                                          <  1:354524/101‑1 (MQ=255)


                                      gcgcgcGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTg                                                         <  1:4108495/101‑1 (MQ=255)


                                           cgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGG.cccc                                                   >  2:3867871/1‑101 (MQ=255)


                                                 tCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGG.ccccccgccg                                             <  1:3091032/101‑6 (MQ=255)


                                                     ggcggcggcGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGAc                                          <  1:1876978/101‑1 (MQ=255)


                                                      gcggcggcgGGGCTGTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGTCATCATTCTTGGCCCCCCGCCGGGACa                                         <  2:2830352/101‑1 (MQ=255)


                                                        gggggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAAt                                       <  1:1679998/98‑1 (MQ=255)


                                                         gcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATc                                      <  2:4226230/101‑1 (MQ=255)


                                                           ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                    >  2:2913539/1‑101 (MQ=255)


                                                            gcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACg                                   <  2:3098337/101‑1 (MQ=255)


                                                            cttccgatctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACg                                   <  2:827031/94‑1 (MQ=255)


                                                             cggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACgg                                  >  2:2417597/1‑101 (MQ=255)


                                                              ggggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgc                                 <  1:3787734/101‑1 (MQ=255)


                                                              ggggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgc                                 >  1:1319674/1‑101 (MQ=255)


                                                              ggggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgc                                 >  1:50404/1‑101 (MQ=255)


                                                               gggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgcc                                <  2:4079203/101‑1 (MQ=255)


                                                                ggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgccg                               <  2:1515937/101‑1 (MQ=255)


                                                                ggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgccg                               <  2:930627/101‑1 (MQ=255)


                                                                  ctctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgccgcc                             >  1:246402/1‑101 (MQ=255)


                                                                    ctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTc                           <  1:1565051/101‑1 (MQ=255)


                                                                    ctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTc                           >  1:67894/1‑101 (MQ=255)


                                                                     ttGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTcc                          >  1:822757/1‑100 (MQ=255)


                                                                      tGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         >  2:975240/1‑101 (MQ=255)


                                                                      tGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCAAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         <  2:258077/101‑1 (MQ=255)


                                                                        cGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTg                       >  2:2179120/1‑101 (MQ=255)


                                                                         gATCCTTCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GCTT.TT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGt                      <  2:2056094/101‑1 (MQ=255)


                                                                         gATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGt                      <  1:3009166/101‑1 (MQ=255)


                                                                         gATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGACCCCCGCCGGGACAATCACGGCCGCCTCATTGt                      >  2:3808510/1‑101 (MQ=255)


                                                                           tCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                    >  1:3698311/1‑101 (MQ=255)


                                                                           tCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                    >  2:3824556/1‑101 (MQ=255)


                                                                           tCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGC.ACGC.GTTT.CCGAACCGGCATCGTTCTTGGCCCCCCGCCGGGTCAATCACGGCCGCCTCATTGTTc                    <  1:3617281/101‑1 (MQ=255)


                                                                             cTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTccc                  >  2:3135731/1‑100 (MQ=255)


                                                                             cTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCg                  >  1:2142521/1‑101 (MQ=255)


                                                                              tGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATTATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGc                 >  1:3958023/1‑101 (MQ=255)


                                                                              tGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGc                 <  1:3877686/101‑1 (MQ=255)


                                                                                 gTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt              >  2:3313142/1‑101 (MQ=255)


                                                                                 gTTTCCGGAGTGGCGACAA.TCC.CGCGCCGCAA.AC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCCCGGCCGCCTCATTGTTCCGCCAt              <  1:2035109/101‑1 (MQ=255)


                                                                                   ttCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTg            <  1:27735/101‑1 (MQ=255)


                                                                                    tCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGc           <  2:733127/101‑1 (MQ=255)


                                                                                        gATTGGCGACAACC.C.CGCGCCGCAA.GC.G.TTCCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCAATTGCGATc       <  2:399230/101‑1 (MQ=255)


                                                                                        gATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATc       <  1:938238/101‑1 (MQ=255)


                                                                                         aTTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      >  1:1739599/1‑101 (MQ=255)


                                                                                         aTTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      >  2:3212836/1‑101 (MQ=255)


                                                                                         aTTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATAATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      <  2:267600/101‑1 (MQ=255)


                                                                                          ttGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaa     >  2:12378/1‑101 (MQ=255)


                                                                                          ttGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaa     <  2:1520063/101‑1 (MQ=255)


                                                                                          ttGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaa     <  2:201029/101‑1 (MQ=255)


                                                                                           tGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa    <  1:2720854/101‑1 (MQ=255)


                                                                                           tGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa    <  2:2540734/101‑1 (MQ=255)


                                                                                             gCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGc  <  2:778283/101‑1 (MQ=255)


AAAATTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.CGCG.CGC.A.GC.G.TT.CCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057725‑2057913

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG