Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,270 +AG 100% coding (107/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,2701.→A100.0% 742.8 184coding (107/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (97/86):  ref base (0/0):  total (98/86)
*NC_0140342,177,2702.→G100.0% 704.3 184coding (107/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (97/86):  ref base (1/0):  total (98/86)

Only 100 of 206 total aligned reads displayed.

CTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGGCCCC  >  NC_014034/2177170‑2177354
                                                                                                      ||                                                                                         
 tGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGa.aaaa                                                                                            >  2:2160101/1‑100 (MQ=255)
  gCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGa.aaaag                                                                                           <  2:635653/101‑3 (MQ=255)
  gCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGa.aaaag                                                                                           >  2:1041953/1‑99 (MQ=255)
  gCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGa.aaaag                                                                                           <  2:1578381/101‑3 (MQ=255)
   cGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAA..agg                                                                                        <  1:3907020/101‑1 (MQ=255)
     tttCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAA..agggg                                                                                      >  2:3606978/1‑99 (MQ=255)
     tttCCACATCGCCACCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAA..agggg                                                                                      <  1:3653995/101‑3 (MQ=255)
          acaTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTg..gt                                                                                 <  1:1252476/101‑1 (MQ=255)
          acaTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTg..gt                                                                                 >  1:3760501/1‑101 (MQ=255)
           caTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTg..gtg                                                                                <  2:3629329/101‑2 (MQ=255)
            aTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGCCGACAAAG.GGGTTg..gtgt                                                                               >  1:1166844/1‑99 (MQ=255)
              cGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTg..gtgtcg                                                                             >  2:235484/1‑97 (MQ=255)
              cGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTg..gtgtcg                                                                             <  1:3271009/101‑5 (MQ=255)
               gCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTg..gtgtcgg                                                                            >  1:2959369/1‑96 (MQ=255)
               gCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTg..gtgtcgg                                                                            >  1:2665658/1‑96 (MQ=255)
                 cATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGCCGA.AAAAGGGGTTg..gtggcggtc                                                                          >  1:3312709/1‑94 (MQ=255)
                 cATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTg..gtgtcggtc                                                                          >  1:2157363/1‑94 (MQ=255)
                 cATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTg..gtgtcggtc                                                                          <  1:2463000/101‑8 (MQ=255)
                  aTCGATCTTGCCCGCATAGGTCGGGACCACCAGCCCCAGCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTg..gtgtcggtct                                                                         <  1:1110363/101‑9 (MQ=255)
                   tCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTg..gtgtcggtctt                                                                        >  2:889190/1‑92 (MQ=255)
                   tCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTg..gtgtcggtctt                                                                        <  2:382624/101‑10 (MQ=255)
                    cGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTt.g                                                                        <  2:1203085/101‑1 (MQ=255)
                       tCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTt.ggtg                                                                     <  2:2516982/101‑4 (MQ=255)
                        cTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGGCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTt.ggtga                                                                    <  2:685110/101‑5 (MQ=255)
                           gCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTt.ggtgatca                                                                 >  1:1193772/1‑94 (MQ=255)
                              cGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTt.ggtgatcagac                                                              <  1:235522/101‑11 (MQ=255)
                              cGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTt.ggtgatcagac                                                              <  1:909857/101‑11 (MQ=255)
                              cGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGATGTCGGTCTt.ggtgatcagac                                                              <  1:2407019/101‑11 (MQ=255)
                                 aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGAtcagacagc                                                            >  1:3521129/1‑100 (MQ=255)
                                   aGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGcc                                                          >  2:1788005/1‑101 (MQ=255)
                                   aGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGcc                                                          >  1:2501286/1‑101 (MQ=255)
                                    tgTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGccc                                                         <  2:2424845/100‑1 (MQ=255)
                                    ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGccc                                                         >  2:2828721/1‑101 (MQ=255)
                                    ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGccc                                                         <  2:3760244/101‑1 (MQ=255)
                                    ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGccc                                                         <  2:1193678/101‑1 (MQ=255)
                                     gTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGcccc                                                        <  2:1938240/101‑1 (MQ=255)
                                      tCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCg                                                       <  1:570016/101‑1 (MQ=255)
                                       cGAGGGGACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCTCCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCgg                                                      <  1:701392/101‑1 (MQ=255)
                                       cGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCgg                                                      <  1:4130660/101‑1 (MQ=255)
                                        gAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGt                                                     >  1:2741483/1‑101 (MQ=255)
                                           agCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTc                                                  <  2:3113942/100‑1 (MQ=255)
                                            gCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCg                                                 <  2:2485221/101‑1 (MQ=255)
                                            gCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCg                                                 <  2:2665452/101‑1 (MQ=255)
                                            gCACCAGCCCCAACTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCg                                                 >  1:2288827/1‑101 (MQ=255)
                                               ccAGCCCCATCTCGGTCGCCTTTGCCGAAGGACCTTCATTCAACTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGcc                                              <  2:1684614/101‑1 (MQ=255)
                                               ccAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGcc                                              <  2:2262830/101‑1 (MQ=255)
                                                cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCg                                             >  2:1532239/1‑101 (MQ=255)
                                                cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCg                                             >  1:1402869/1‑101 (MQ=255)
                                                cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCg                                             >  1:1594567/1‑101 (MQ=255)
                                                 aGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.a                                           <  2:106494/101‑1 (MQ=255)
                                                  gCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aa                                          <  2:3795886/101‑2 (MQ=255)
                                                   ccccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aag                                         >  2:3290683/1‑99 (MQ=255)
                                                     ccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagcc                                       >  1:1345962/1‑97 (MQ=255)
                                                       aTCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAgccgc                                      >  1:2197503/1‑101 (MQ=255)
                                                       aTCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAgccgc                                      >  1:1847932/1‑101 (MQ=255)
                                                        tctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAgccgcc                                     >  2:1780715/1‑101 (MQ=255)
                                                         ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGccc                                    >  2:73716/1‑101 (MQ=255)
                                                          tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGcccc                                   <  2:2556287/101‑1 (MQ=255)
                                                          tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGcccc                                   <  2:305056/101‑1 (MQ=255)
                                                          ccGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGcccc                                   <  2:3977809/100‑1 (MQ=255)
                                                            ggTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCgg                                 >  1:3117233/1‑101 (MQ=255)
                                                            ggTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCgg                                 >  1:1692568/1‑101 (MQ=255)
                                                               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                              <  1:3209925/101‑1 (MQ=255)
                                                               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                              <  2:185072/101‑1 (MQ=255)
                                                                gCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAg                             <  1:280607/101‑1 (MQ=255)
                                                                 ccTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgc                            <  2:2874585/101‑1 (MQ=255)
                                                                 ccTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgc                            >  2:1016774/1‑101 (MQ=255)
                                                                  cTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcg                           >  1:1417330/1‑101 (MQ=255)
                                                                   tttGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcgc                          >  1:3365034/1‑101 (MQ=255)
                                                                   tttGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcgc                          >  1:492361/1‑101 (MQ=255)
                                                                    ttGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGcgcg                         >  2:3923690/1‑101 (MQ=255)
                                                                     tGCCGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                        <  2:2141293/101‑1 (MQ=255)
                                                                      gCCGAAGCACCTGCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGa                       <  2:1902434/101‑1 (MQ=255)
                                                                       ccGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                      <  1:3807382/101‑1 (MQ=255)
                                                                        cGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                     <  2:3520876/101‑1 (MQ=255)
                                                                        cGAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                     <  2:2055213/101‑1 (MQ=255)
                                                                         gAAGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGACCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaaa                    >  1:3470403/1‑101 (MQ=255)
                                                                          aaGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                   >  1:2039464/1‑101 (MQ=255)
                                                                          aaGCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                   >  2:590890/1‑101 (MQ=255)
                                                                            gCACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGc                 >  1:1462119/1‑101 (MQ=255)
                                                                             cACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaaagatc                >  2:4146818/1‑97 (MQ=255)
                                                                             cACCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGcc                <  2:2501102/101‑1 (MQ=255)
                                                                              aCCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa               >  1:2849029/1‑101 (MQ=255)
                                                                              aCCTTCCTTCATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa               <  2:1673784/101‑1 (MQ=255)
                                                                                cttccttcATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg            >  2:412221/1‑101 (MQ=255)
                                                                                cttccttcATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg            <  2:3444543/101‑1 (MQ=255)
                                                                                cttccttcATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg            <  1:73731/101‑1 (MQ=255)
                                                                                cttccttcATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg            <  2:1462017/101‑1 (MQ=255)
                                                                                 ttccttcATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.ggg           >  1:526232/1‑100 (MQ=255)
                                                                                 ttccttcATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.ggg           <  2:1342847/101‑2 (MQ=255)
                                                                                  tccttcATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc          <  1:4151723/101‑3 (MQ=255)
                                                                                  tccttcATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc          >  1:1487639/1‑99 (MQ=255)
                                                                                    cttcATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcag        >  1:835945/1‑97 (MQ=255)
                                                                                     ttcATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga       >  1:1731096/1‑96 (MQ=255)
                                                                                     ttcATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga       >  1:1136049/1‑96 (MQ=255)
                                                                                       cATCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg      >  1:720550/1‑101 (MQ=255)
                                                                                        aTCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGc     >  2:3591778/1‑101 (MQ=255)
                                                                                        aTCTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGc     >  1:160820/1‑101 (MQ=255)
                                                                                          cTTGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGccc   <  1:1042047/101‑1 (MQ=255)
                                                                                           ttGACGA.AAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGcccc  >  2:1414717/1‑101 (MQ=255)
                                                                                                      ||                                                                                         
CTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGA.AAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGGCCCC  >  NC_014034/2177170‑2177354

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG