Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,342 +G 100% coding (35/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,3421.→G100.0% 526.4 140coding (35/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (83/57):  ref base (0/0):  total (83/57)

Only 100 of 164 total aligned reads displayed.

ACCTTCCTTCATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCC.G.A.GCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGA.GG.CCC.CAACAGGGCCA.G.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGA  >  NC_014034/2177248‑2177435
                                                                                                       |                                                                                                  
aCCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCa                                                                                                     <  2:1673784/101‑1 (MQ=255)
aCCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCa                                                                                                     >  1:2849029/1‑101 (MQ=255)
  cttccttcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                                                                                  >  2:3552498/1‑101 (MQ=255)
  cttccttcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                                                                                  <  2:3444543/101‑1 (MQ=255)
  cttccttcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                                                                                  <  2:1462017/101‑1 (MQ=255)
    tccttcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                                                                                >  1:1487639/1‑99 (MQ=255)
      cttcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcag                                                                                              <  2:3735544/101‑5 (MQ=255)
      cttcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcag                                                                                              >  1:835945/1‑97 (MQ=255)
       ttcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                                                                                             >  2:529750/1‑96 (MQ=255)
       ttcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                                                                                             >  1:1136049/1‑96 (MQ=255)
       ttcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                                                                                             >  1:1731096/1‑96 (MQ=255)
       tgcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                                                                                             <  1:986364/99‑6 (MQ=255)
        tcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCaga.g                                                                                            <  2:1196356/101‑1 (MQ=255)
         cATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.gg                                                                                           >  1:3348747/1‑101 (MQ=255)
          aTCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GG.c                                                                                         >  1:160820/1‑101 (MQ=255)
            cTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GG.ccc                                                                                       <  1:1042047/101‑1 (MQ=255)
             ttGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GG.ccc.c                                                                                     >  2:1414717/1‑101 (MQ=255)
                       aggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGcc                                                                            >  2:4114915/11‑101 (MQ=255)
                        ggggttgttgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCa                                                                           <  2:1286954/92‑1 (MQ=255)
                        ggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCa                                                                           <  1:2881339/92‑1 (MQ=255)
                           gttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.G.tt                                                                      >  2:1012826/7‑101 (MQ=255)
                           gttggagtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.G.tt                                                                      >  2:387955/7‑101 (MQ=255)
                             tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.G.tttg                                                                    <  1:3025455/97‑3 (MQ=255)
                             tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.G.tttg                                                                    >  1:2914145/5‑99 (MQ=255)
                               gtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.G.tttgcg                                                                  >  2:2701122/3‑97 (MQ=255)
                                tgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.G.tttgcgc                                                                 >  2:2336119/2‑96 (MQ=255)
                                 gtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGca                                                                 >  2:3848462/1‑101 (MQ=255)
                                  tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGcat                                                                >  1:1320930/1‑101 (MQ=255)
                                  tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGcat                                                                <  2:1847812/101‑1 (MQ=255)
                                   cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGcatc                                                               >  2:243539/1‑101 (MQ=255)
                                   cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGcatc                                                               <  2:2913926/101‑1 (MQ=255)
                                   cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGcatc                                                               <  1:889275/101‑1 (MQ=255)
                                   cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGcatc                                                               >  2:827776/1‑101 (MQ=255)
                                       cttggtgatcagacagGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCAtcatca                                                           >  1:2384536/10‑101 (MQ=255)
                                       cttggtgatcagacagGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCAtcatca                                                           >  1:1268548/10‑101 (MQ=255)
                                       cttggtgatcagacagGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCAtcatca                                                           >  1:2846548/10‑101 (MQ=255)
                                        ttggtgatcagacagGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCAc                                                          <  2:1345862/93‑1 (MQ=255)
                                        ttggtgatcagacagGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCAc                                                          <  2:1650331/93‑1 (MQ=255)
                                         tggtgatcagacagGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACg                                                         >  2:167243/8‑101 (MQ=255)
                                         tggtgatcagacagGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACg                                                         >  2:4078439/8‑101 (MQ=255)
                                           gtgatcagacagGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGt                                                       >  2:4226676/6‑101 (MQ=255)
                                           gtgatcagacagGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGt                                                       >  1:2792221/6‑101 (MQ=255)
                                            tgatcagacagGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGtc                                                      <  1:2916332/97‑1 (MQ=255)
                                             gatcagacagGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGtct                                                     >  1:2432239/4‑101 (MQ=255)
                                              atcagacagGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGtctc                                                    >  2:4225838/3‑101 (MQ=255)
                                               ccagcccggCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGtctct                                                   <  1:2191529/94‑1 (MQ=255)
                                                cagacagGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGtctctc                                                  >  2:127762/1‑101 (MQ=255)
                                                   acagGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTctcctc                                               >  2:1198035/1‑101 (MQ=255)
                                                   acagGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTctcctc                                               >  2:868082/1‑101 (MQ=255)
                                                    ccggCCCCGGTGTCTGCCGG.A.GCCCCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCtcctcc                                              <  1:1284279/99‑1 (MQ=255)
                                                    cagGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCtcctcc                                              <  1:2640181/101‑1 (MQ=255)
                                                    cagGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCtcctcc                                              >  1:425746/1‑101 (MQ=255)
                                                     agGCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GG.CCCACAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTccc                                             <  2:501078/101‑1 (MQ=255)
                                                      ggCCCCGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCg                                            >  1:4224755/1‑101 (MQ=255)
                                                        ccccGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGtt                                          <  2:2772001/101‑1 (MQ=255)
                                                         cccGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGttt                                         >  2:3512191/1‑101 (MQ=255)
                                                         cccGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGttt                                         <  2:2288659/101‑1 (MQ=255)
                                                         cccGGTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGttt                                         >  2:2117932/1‑101 (MQ=255)
                                                          ccGGTTTCAGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTc                                        <  2:3331623/101‑1 (MQ=255)
                                                             gTTTCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt                                     >  1:81962/1‑101 (MQ=255)
                                                              tttCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTg                                    >  1:3584055/1‑101 (MQ=255)
                                                               ttCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGt                                   >  2:2944952/1‑101 (MQ=255)
                                                                tCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTc                                  <  2:3364789/101‑1 (MQ=255)
                                                                tCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTc                                  <  1:590954/101‑1 (MQ=255)
                                                                tCGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTc                                  >  1:2732072/1‑101 (MQ=255)
                                                                 cGGCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCg                                 >  2:1771111/1‑101 (MQ=255)
                                                                 cGGCC.G.AGGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCg                                 <  2:3117002/101‑1 (MQ=255)
                                                                  ggCC.GAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCgg                                >  2:2766348/1‑101 (MQ=255)
                                                                       gaa.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                            <  1:412272/99‑1 (MQ=255)
                                                                       gaa.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                            >  2:4155526/3‑101 (MQ=255)
                                                                       gaa.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                            >  1:4006385/3‑101 (MQ=255)
                                                                       gaa.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                            >  2:2570071/3‑101 (MQ=255)
                                                                       gaa.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                            >  1:3776265/3‑101 (MQ=255)
                                                                       gaa.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                            >  2:808561/3‑101 (MQ=255)
                                                                        aa.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGG.CCC.CCACAGGGGCAGG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCCCTGTCCGccccc                           >  1:2716474/2‑100 (MQ=255)
                                                                        aa.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcct                           <  2:2959147/100‑1 (MQ=255)
                                                                              gccCCGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                      <  1:2132260/101‑1 (MQ=255)
                                                                               ccccGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCg                     <  1:4155788/101‑1 (MQ=255)
                                                                                cccGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCgg                    <  1:148606/101‑1 (MQ=255)
                                                                                cccGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCgg                    >  2:565481/1‑101 (MQ=255)
                                                                                 ccGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCggg                   >  1:2175480/1‑101 (MQ=255)
                                                                                  cGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCTTCCCGTTTCACTGTCGGCCCCTCCTCCgggg                  <  1:3749173/101‑1 (MQ=255)
                                                                                  cGGCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCgggg                  >  1:3966972/1‑101 (MQ=255)
                                                                                   ggCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCggggn                 >  1:3887748/1‑100 (MQ=255)
                                                                                   ggCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGc                 >  1:1916388/1‑101 (MQ=255)
                                                                                    gCCAGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCt                <  1:727618/101‑1 (MQ=255)
                                                                                       aGCGCGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCgg             <  2:1320830/101‑1 (MQ=255)
                                                                                        gcgcGGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGa            <  2:47395/101‑1 (MQ=255)
                                                                                           cgGAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaa         <  1:2336287/101‑1 (MQ=255)
                                                                                            ggAAAGCGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaaa        <  1:3051605/101‑1 (MQ=255)
                                                                                            ggAAAACGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaaa        <  1:594165/101‑1 (MQ=255)
                                                                                               aaaCGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTc     >  1:2153387/1‑101 (MQ=255)
                                                                                               aaaCGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GCTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTc     >  2:2588688/1‑101 (MQ=255)
                                                                                                aaCGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCt    <  1:1788124/101‑1 (MQ=255)
                                                                                                 aCGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg   <  1:3771139/101‑1 (MQ=255)
                                                                                                 aCGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg   >  2:3214754/1‑101 (MQ=255)
                                                                                                 aCGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg   >  2:3046330/1‑101 (MQ=255)
                                                                                                 aCGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg   >  1:4121886/1‑101 (MQ=255)
                                                                                                 aCGCCAGGGCAGA.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg   <  1:529814/101‑1 (MQ=255)
                                                                                                  cGCCAGGGCAGA.GGCCCC.CAACAGGGGCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGa  <  1:3552751/101‑1 (MQ=255)
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ACCTTCCTTCATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCC.G.A.GCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGA.GG.CCC.CAACAGGGCCA.G.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGA  >  NC_014034/2177248‑2177435

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG