Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,350 +C 100% coding (27/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,3501.→C100.0% 537.1 145coding (27/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (81/62):  ref base (1/1):  total (82/63)

Only 100 of 161 total aligned reads displayed.

CATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCC.G.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAG.A.GG.CCC.CAACAGGGCCA.G.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCG  >  NC_014034/2177257‑2177444
                                                                                                        |                                                                                                 
cATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.gg                                                                                                    >  1:720550/1‑101 (MQ=255)
cATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.gg                                                                                                    >  1:3348747/1‑101 (MQ=255)
 aTCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GG.c                                                                                                  >  1:160820/1‑101 (MQ=255)
 aTCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GG.c                                                                                                  >  2:3591778/1‑101 (MQ=255)
  tCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GT.cc                                                                                                 >  2:2385926/1‑101 (MQ=255)
   cTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GG.ccc                                                                                                <  1:1042047/101‑1 (MQ=255)
    ttGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GG.ccc.c                                                                                              >  1:542867/1‑101 (MQ=255)
    ttGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GG.ccc.c                                                                                              >  2:1414717/1‑101 (MQ=255)
    ttGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GG.ccc.c                                                                                              >  1:3468442/1‑101 (MQ=255)
              aggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGcc                                                                                     >  2:4114915/11‑101 (MQ=255)
               ggggttgttgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCa                                                                                    <  2:1286954/92‑1 (MQ=255)
               ggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCa                                                                                    <  1:2881339/92‑1 (MQ=255)
                  gttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.G.tt                                                                               <  2:1271598/95‑1 (MQ=255)
                   ttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.G.ttt                                                                              <  1:170409/96‑2 (MQ=255)
                    tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.G.tttg                                                                             <  1:3025455/97‑3 (MQ=255)
                    tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.G.tttg                                                                             >  1:2914145/5‑99 (MQ=255)
                      gtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.G.tttgcg                                                                           >  2:2701122/3‑97 (MQ=255)
                       tgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.G.tttgcgc                                                                          >  2:2336119/2‑96 (MQ=255)
                        gtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGca                                                                          >  1:3948993/1‑101 (MQ=255)
                         tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGcat                                                                         <  2:1847812/101‑1 (MQ=255)
                         tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGcat                                                                         >  1:1320930/1‑101 (MQ=255)
                          cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGcatc                                                                        <  2:2913926/101‑1 (MQ=255)
                          cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGcatc                                                                        >  2:827776/1‑101 (MQ=255)
                             tcttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCatcatc                                                                     >  2:1700220/11‑101 (MQ=255)
                             tcttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCatcatc                                                                     <  1:847200/91‑1 (MQ=255)
                              cttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCAtcatca                                                                    >  1:2846548/10‑101 (MQ=255)
                              cttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCAtcatca                                                                    >  1:1268548/10‑101 (MQ=255)
                               ttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCAc                                                                   <  2:1345862/93‑1 (MQ=255)
                               ttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCAc                                                                   <  2:1650331/93‑1 (MQ=255)
                                  gtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGt                                                                >  1:2792221/6‑101 (MQ=255)
                                   tgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGtc                                                               <  1:2916332/97‑1 (MQ=255)
                                    gatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGtct                                                              >  1:2432239/4‑101 (MQ=255)
                                     atcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGtctc                                                             >  2:4225838/3‑101 (MQ=255)
                                      ccagcccggCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGtctct                                                            <  1:2191529/94‑1 (MQ=255)
                                       cagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGtctctc                                                           >  2:531621/1‑101 (MQ=255)
                                         gacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTctcct                                                         <  1:937646/101‑1 (MQ=255)
                                          acagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTctcctc                                                        >  2:1198035/1‑101 (MQ=255)
                                          acagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTctcctc                                                        >  2:868082/1‑101 (MQ=255)
                                           ccggCCCCGGTGTCTGCCGG.AGCCCCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCtcctcc                                                       <  1:1284279/99‑1 (MQ=255)
                                           cagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCtcctcc                                                       >  2:3821524/1‑101 (MQ=255)
                                           cagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCtcctcc                                                       >  1:425746/1‑101 (MQ=255)
                                            agGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTccc                                                      <  2:3464686/101‑1 (MQ=255)
                                            agGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTccc                                                      <  2:2510388/101‑1 (MQ=255)
                                            agGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GG.CCCACAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTccc                                                      <  2:501078/101‑1 (MQ=255)
                                             ggCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCg                                                     >  1:4224755/1‑101 (MQ=255)
                                                cccGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGttt                                                  <  2:2288659/101‑1 (MQ=255)
                                                 ccGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTc                                                 >  1:2117611/1‑101 (MQ=255)
                                                 ccGGTTTCAGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTc                                                 <  2:3331623/101‑1 (MQ=255)
                                                  cGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGTGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCa                                                <  2:3157990/101‑1 (MQ=255)
                                                    gTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt                                              >  1:81962/1‑101 (MQ=255)
                                                    gTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt                                              >  1:3702658/1‑101 (MQ=255)
                                                      ttCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGt                                            >  2:2944952/1‑101 (MQ=255)
                                                       tCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTc                                           >  1:2732072/1‑101 (MQ=255)
                                                        cGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAA.GC.CCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCg                                          >  2:2211460/1‑101 (MQ=255)
                                                        cGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCg                                          >  2:4069498/1‑101 (MQ=255)
                                                         ggCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCgg                                         >  2:2766348/1‑101 (MQ=255)
                                                              gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                                     >  2:1907006/3‑101 (MQ=255)
                                                              gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                                     >  2:3385764/3‑101 (MQ=255)
                                                              gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                                     >  2:4155526/3‑101 (MQ=255)
                                                              gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                                     >  2:808561/3‑101 (MQ=255)
                                                              gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                                     <  1:412272/99‑1 (MQ=255)
                                                              gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                                     >  1:3776265/3‑101 (MQ=255)
                                                               aaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.AGGG.CCC.CCACAGGGGCAGG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCCCTGTCCGccccc                                    >  1:2716474/2‑100 (MQ=255)
                                                               aaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcct                                    <  2:2959147/100‑1 (MQ=255)
                                                                     ccccGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCg                              <  1:4155788/101‑1 (MQ=255)
                                                                      cccGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCgg                             <  1:148606/101‑1 (MQ=255)
                                                                      cccGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCgg                             <  2:2846329/101‑1 (MQ=255)
                                                                       ccGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCggg                            <  1:243584/101‑1 (MQ=255)
                                                                        cGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCTTCCCGTTTCACTGTCGGCCCCTCCTCCgggg                           <  1:3749173/101‑1 (MQ=255)
                                                                        cGGCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCgggg                           >  1:3966972/1‑101 (MQ=255)
                                                                         ggCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCggggn                          >  1:3887748/1‑100 (MQ=255)
                                                                         ggCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGc                          >  1:1916388/1‑101 (MQ=255)
                                                                          gCCAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCt                         >  2:128452/1‑101 (MQ=255)
                                                                           ccAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTc                        <  2:425689/101‑1 (MQ=255)
                                                                           ccAGCGCGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTc                        <  1:4008512/101‑1 (MQ=255)
                                                                              gcgcGGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGa                     <  2:47395/101‑1 (MQ=255)
                                                                                 cgGAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaa                  <  1:2336287/101‑1 (MQ=255)
                                                                                  ggAAAGCGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaaa                 <  1:3051605/101‑1 (MQ=255)
                                                                                  ggAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaaa                 >  1:2360371/1‑101 (MQ=255)
                                                                                  ggAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaaa                 <  1:594165/101‑1 (MQ=255)
                                                                                   gAAAACGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAg                >  2:1670503/1‑101 (MQ=255)
                                                                                     aaaCGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTc              >  1:2153387/1‑101 (MQ=255)
                                                                                     aaaCGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GCTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTc              >  2:2588688/1‑101 (MQ=255)
                                                                                      aaCGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCt             >  1:2156881/1‑101 (MQ=255)
                                                                                       aCGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg            >  2:3214754/1‑101 (MQ=255)
                                                                                       aCGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg            >  2:3046330/1‑101 (MQ=255)
                                                                                       aCGCCAGGGCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg            <  1:3771139/101‑1 (MQ=255)
                                                                                        cGCCAGGGCAG.A.GGCCCC.CAACAGGGGCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGa           <  1:3552751/101‑1 (MQ=255)
                                                                                         gccagggCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAc          <  2:636252/96‑1 (MQ=255)
                                                                                         gccagggCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAc          >  1:1002531/6‑101 (MQ=255)
                                                                                          ccagggCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAcc         >  1:1511665/5‑101 (MQ=255)
                                                                                          ccagggCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCACATCCTCCCGGTCTCTCCTCCCGTTTCACTGCCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAcc         <  2:1353111/97‑1 (MQ=255)
                                                                                           cagggCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAccc        <  2:3947035/98‑1 (MQ=255)
                                                                                           caggccAG.A.GGCCCC.CAACAGGGCCA.GTTTGGGCATCATCGCGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAccc        <  2:2731866/96‑1 (MQ=255)
                                                                                              ggCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAg     <  2:2432063/101‑1 (MQ=255)
                                                                                              ggCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAg     >  1:2715736/1‑101 (MQ=255)
                                                                                               gCAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGt    >  2:2571821/1‑101 (MQ=255)
                                                                                                cAG.A.GGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTc   <  2:3875426/101‑1 (MQ=255)
                                                                                                 ag.a.gGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCg  >  1:1869710/1‑101 (MQ=255)
                                                                                                 ag.a.gGCCCC.CAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCg  >  1:3103131/1‑101 (MQ=255)
                                                                                                        |                                                                                                 
CATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCC.G.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAG.A.GG.CCC.CAACAGGGCCA.G.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCG  >  NC_014034/2177257‑2177444

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG