Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,365 +T 100% coding (12/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,3651.→T100.0% 613.4 160coding (12/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (87/71):  ref base (0/1):  total (88/72)

Only 100 of 182 total aligned reads displayed.

AAGGGTTGGTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.A.GCCGCCCCGGCCAGCGCGGAAAACGCCA.G.GCAG.A.GG.CCCCAACAGGGCCA.G.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTCAATAT  >  NC_014034/2177269‑2177460
                                                                                                          |                                                                                               
gggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.g                                                                                                  <  2:835864/93‑1 (MQ=255)
 ggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.G.t                                                                                                <  1:4223058/94‑1 (MQ=255)
  gttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.G.tt                                                                                               <  2:1271598/95‑1 (MQ=255)
  gttggagtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.G.tt                                                                                               >  2:387955/7‑101 (MQ=255)
   ttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.G.ttt                                                                                              <  1:170409/96‑2 (MQ=255)
    tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.G.tttg                                                                                             >  1:2914145/5‑99 (MQ=255)
    tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.G.tttg                                                                                             <  1:3025455/97‑3 (MQ=255)
     ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.G.tttgc                                                                                            <  2:492304/98‑4 (MQ=255)
     ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.G.tttgc                                                                                            >  1:1349660/4‑98 (MQ=255)
      gtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.G.tttgcg                                                                                           >  2:2701122/3‑97 (MQ=255)
        gtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGca                                                                                          >  2:3848462/1‑101 (MQ=255)
        gtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGca                                                                                          >  2:80027/1‑101 (MQ=255)
        gtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGca                                                                                          >  1:3948993/1‑101 (MQ=255)
         tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGcat                                                                                         <  2:1847812/101‑1 (MQ=255)
          cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGcatc                                                                                        >  2:243539/1‑101 (MQ=255)
          cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGcatc                                                                                        <  2:2913926/101‑1 (MQ=255)
             tcttggtgatcagacagGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCatcatc                                                                                     <  1:847200/91‑1 (MQ=255)
              cttggtgatcagacagGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCatcatcc                                                                                    >  2:1552697/10‑100 (MQ=255)
              cttggtgatcagacagGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCAtcatca                                                                                    >  1:1268548/10‑101 (MQ=255)
              cttggtgatcagacagGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCAtcatca                                                                                    >  1:2846548/10‑101 (MQ=255)
               ttggtgatcagacagGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCAc                                                                                   <  2:1345862/93‑1 (MQ=255)
               ttggtgatcagacagGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCAc                                                                                   <  2:1650331/93‑1 (MQ=255)
                tggtgatcagacagGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACg                                                                                  >  2:4078439/8‑101 (MQ=255)
                  gtgatcagacagGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGt                                                                                >  1:2792221/6‑101 (MQ=255)
                    gatcagacagGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGtct                                                                              >  1:2432239/4‑101 (MQ=255)
                     atcagacagGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGtctc                                                                             >  2:4225838/3‑101 (MQ=255)
                      ccagcccggCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGtctct                                                                            <  1:2191529/94‑1 (MQ=255)
                       cagacagGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGtctctc                                                                           >  2:531621/1‑101 (MQ=255)
                         gacagGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTctcct                                                                         >  1:1903828/1‑101 (MQ=255)
                         gacagGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTctcct                                                                         <  1:937646/101‑1 (MQ=255)
                           cagGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCtcctcc                                                                       <  1:2640181/101‑1 (MQ=255)
                           cagGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCtcctcc                                                                       >  1:425746/1‑101 (MQ=255)
                            agGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTccc                                                                      <  2:3464686/101‑1 (MQ=255)
                            agGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTccc                                                                      <  2:2510388/101‑1 (MQ=255)
                             ggCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCg                                                                     >  1:3753264/1‑101 (MQ=255)
                               ccccGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGtt                                                                   <  2:2772001/101‑1 (MQ=255)
                                cccGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGttt                                                                  >  2:2117932/1‑101 (MQ=255)
                                cccGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGttt                                                                  <  2:2288659/101‑1 (MQ=255)
                                 ccGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTc                                                                 >  1:2117611/1‑101 (MQ=255)
                                  cGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGTGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCa                                                                <  2:3157990/101‑1 (MQ=255)
                                    gTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt                                                              >  1:3702658/1‑101 (MQ=255)
                                    gTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt                                                              >  1:81962/1‑101 (MQ=255)
                                     tttCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTg                                                             >  1:3584055/1‑101 (MQ=255)
                                      ttCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGt                                                            >  2:2944952/1‑101 (MQ=255)
                                       tCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTc                                                           >  1:2732072/1‑101 (MQ=255)
                                       tCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTc                                                           <  2:3364789/101‑1 (MQ=255)
                                        cGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAGAA.GC.CCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCg                                                          >  2:2211460/1‑101 (MQ=255)
                                        cGGCCG.AGGCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCg                                                          <  2:3117002/101‑1 (MQ=255)
                                         ggCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCgg                                                         >  1:1547620/1‑101 (MQ=255)
                                         ggCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCgg                                                         >  2:2766348/1‑101 (MQ=255)
                                             gaa.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                                                     >  1:1317285/3‑101 (MQ=255)
                                             gaa.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                                                     <  1:412272/99‑1 (MQ=255)
                                             gaa.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                                                     >  1:4006385/3‑101 (MQ=255)
                                             gaa.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                                                     >  2:4155526/3‑101 (MQ=255)
                                              aa.GCCGCCCCGGCCAGCGCGGAAAACGCCAGG.GCAG.AGGG.CCCCCACAGGGGCAGG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCCCTGTCCGccccc                                                    >  1:2716474/2‑100 (MQ=255)
                                                      cccGGCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCgg                                             <  1:148606/101‑1 (MQ=255)
                                                         ggCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCggggn                                          >  1:3887748/1‑100 (MQ=255)
                                                          gCCAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCt                                         >  2:128452/1‑101 (MQ=255)
                                                           ccAGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTc                                        <  2:425689/101‑1 (MQ=255)
                                                             aGCGCGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCgg                                      >  1:2249380/1‑101 (MQ=255)
                                                              gcgcGGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGa                                     <  2:2983646/101‑1 (MQ=255)
                                                               cgcgGAAAGCGCCA.GCGCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCTCGGCCTCTCCTCCCGGTTCACTGTCGGCCCCGCCTCCGGGGCTCGGAc                                    <  2:3312468/101‑1 (MQ=255)
                                                                 cgGAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaa                                  <  1:2336287/101‑1 (MQ=255)
                                                                  ggAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaaa                                 <  1:594165/101‑1 (MQ=255)
                                                                   gAAAACGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAg                                >  2:1670503/1‑101 (MQ=255)
                                                                      aaCGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCt                             >  1:2156881/1‑101 (MQ=255)
                                                                      aaCGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCt                             <  1:1788124/101‑1 (MQ=255)
                                                                       aCGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg                            <  1:3771139/101‑1 (MQ=255)
                                                                       aCGCCAGG.GCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg                            >  1:4121886/1‑101 (MQ=255)
                                                                         gccagg.gCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGGTCGGACACCGTCTGAc                          >  2:2353395/6‑101 (MQ=255)
                                                                         gccagg.gCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAc                          <  2:636252/96‑1 (MQ=255)
                                                                         gccagg.gCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAc                          >  1:1002531/6‑101 (MQ=255)
                                                                          ccagg.gCAG.A.GGCCCCCAACAGGGCCA.GTTTGCACATCCTCCCGGTCTCTCCTCCCGTTTCACTGCCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAcc                         <  2:1353111/97‑1 (MQ=255)
                                                                           cagg.gCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAccc                        >  2:932514/4‑101 (MQ=255)
                                                                           cagg.gCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAccc                        <  1:3041379/98‑1 (MQ=255)
                                                                           cagg.gCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAccc                        <  1:3521418/98‑1 (MQ=255)
                                                                            caggccAG.A.GGCCCCCAACAGGGCCA.GTTTGGGCATCATCGCGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAccc                        <  2:2731866/96‑1 (MQ=255)
                                                                              g.gCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAg                     >  1:2715736/1‑101 (MQ=255)
                                                                                gCAG.A.GGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGt                    >  2:2571821/1‑101 (MQ=255)
                                                                                  ag.a.gGCCCCCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCg                  >  1:3103131/1‑101 (MQ=255)
                                                                                     gaggcccccAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCgg                 >  2:3599243/6‑101 (MQ=255)
                                                                                     gaggcccccAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCgg                 <  1:55768/96‑1 (MQ=255)
                                                                                      aggcccccAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGgc                >  1:2151109/5‑101 (MQ=255)
                                                                                      aggcccccAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGgc                <  2:1692453/97‑1 (MQ=255)
                                                                                       ggcccccAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCCGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGgcg               >  2:3489173/4‑101 (MQ=255)
                                                                                         cccccAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCCcc             >  1:2374980/2‑101 (MQ=255)
                                                                                          ccccAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGccc            >  1:119789/1‑101 (MQ=255)
                                                                                           cccAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGcccc           >  2:1724412/1‑101 (MQ=255)
                                                                                           cccAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGcccc           <  2:81938/101‑1 (MQ=255)
                                                                                           cccAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGcccc           <  2:2597468/101‑1 (MQ=255)
                                                                                             cAACAGGGCCA.GTTTGCGTATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCgg         <  2:719360/101‑1 (MQ=255)
                                                                                             cAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCgg         >  1:4132159/1‑101 (MQ=255)
                                                                                              aaCAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGt        >  1:628204/1‑101 (MQ=255)
                                                                                              aaCAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGt        <  2:3610990/101‑1 (MQ=255)
                                                                                              aaCAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGt        <  2:2039326/101‑1 (MQ=255)
                                                                                                cAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTCa      <  2:1071814/101‑1 (MQ=255)
                                                                                                cAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTCa      >  2:1240975/1‑101 (MQ=255)
                                                                                                  gggCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAGCGGAGACGacgcacacgtcaca    >  2:4068944/1‑91 (MQ=255)
                                                                                                  gggCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTCAat    <  1:140295/101‑1 (MQ=255)
                                                                                                    gCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTCAatat  <  1:2118077/101‑1 (MQ=255)
                                                                                                          |                                                                                               
AAGGGTTGGTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.A.GCCGCCCCGGCCAGCGCGGAAAACGCCA.G.GCAG.A.GG.CCCCAACAGGGCCA.G.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTCAATAT  >  NC_014034/2177269‑2177460

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG