Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,180,161 +C 100% coding (850/1314 nt) RCAP_rcc02022 → polyols ABC transporter periplasmic polyols‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,180,1611.→C100.0% 658.1 176coding (850/1314 nt)RCAP_rcc02022polyols ABC transporter periplasmic polyols‑binding protein
Reads supporting (aligned to +/- strand):  new base (81/95):  ref base (0/0):  total (81/95)

Only 100 of 207 total aligned reads displayed.

CTTCCAGCAGGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCTGGC.CCGAACAAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCG  >  NC_014034/2180061‑2180256
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ctTCCAGCAGGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGtcggcttcg                                                                                                    <  1:289718/101‑1 (MQ=255)
  tCCAGCAGGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGtcggcttcgcg                                                                                                  >  2:4160322/1‑99 (MQ=255)
   ccAGCAGGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGtcggcttcgcgc                                                                                                 >  1:3596006/1‑98 (MQ=255)
    cagcagGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGtcggcttcgcgct                                                                                                >  1:2109080/1‑97 (MQ=255)
     agcagGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGtcggcttcgcgctg                                                                                               <  1:1060698/101‑6 (MQ=255)
     agcagGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGtcggcttcgcgctg                                                                                               <  1:1949756/101‑6 (MQ=255)
       cagGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGc                                                                                             >  2:3570894/1‑101 (MQ=255)
        agGGCCATTGCGGCATGTGGATCTAGGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACGCGGCCGTGGCCGCTCATGTCGGATTCGCGCTGGcg                                                                                            >  2:1654533/1‑100 (MQ=255)
        agGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGcg                                                                                            >  1:1567145/1‑100 (MQ=255)
        agGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGcg                                                                                            >  1:3039847/1‑100 (MQ=255)
        agGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGcg                                                                                            <  2:1495480/101‑2 (MQ=255)
        agGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGcg                                                                                            >  1:2551405/1‑100 (MQ=255)
          ggCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGcgcc                                                                                          <  2:1116758/101‑4 (MQ=255)
            ccATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGcgccga                                                                                        >  2:3160246/1‑96 (MQ=255)
            ccATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGcgccga                                                                                        >  2:4014425/1‑96 (MQ=255)
              aTTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAc                                                                                      >  2:3677276/1‑101 (MQ=255)
              aTTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTCACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAc                                                                                      <  2:2262992/101‑1 (MQ=255)
               ttGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACa                                                                                     <  2:4049330/101‑1 (MQ=255)
               ttGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACa                                                                                     <  2:199603/101‑1 (MQ=255)
               ttGCGGCATGGGGATCGATGCGGCCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACa                                                                                     <  2:518009/101‑1 (MQ=255)
                tGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACaa                                                                                    >  2:2055008/1‑101 (MQ=255)
                 gCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAg                                                                                   <  1:3987462/101‑1 (MQ=255)
                  cGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAgg                                                                                  >  1:2862827/1‑101 (MQ=255)
                     cATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGG.ccg                                                                              >  1:2490467/1‑99 (MQ=255)
                      aTGTGGATCGATGCGACCGTGGCCGCGTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGG.ccgg                                                                             <  1:3553489/101‑4 (MQ=255)
                      aTGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGG.ccgg                                                                             >  2:3798379/1‑98 (MQ=255)
                       tgtgGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGG.ccggt                                                                            <  1:4201443/101‑5 (MQ=255)
                       tgtgGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGG.ccggt                                                                            <  2:2417580/101‑5 (MQ=255)
                        gtgGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGG.ccggtg                                                                           <  2:3308228/101‑6 (MQ=255)
                         tgGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGG.ccggtgc                                                                          >  2:4210755/1‑95 (MQ=255)
                          ggATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTc                                                                          <  1:812606/101‑1 (MQ=255)
                          ggATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTc                                                                          <  1:1654836/101‑1 (MQ=255)
                          ggATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTc                                                                          <  1:2973167/101‑1 (MQ=255)
                          ggATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTc                                                                          >  1:2302459/1‑101 (MQ=255)
                            aTCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTcgc                                                                        >  1:1882019/1‑99 (MQ=255)
                            aTCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTcgc                                                                        >  2:191915/1‑99 (MQ=255)
                            aTCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTcgc                                                                        >  1:3998540/1‑99 (MQ=255)
                             tCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTcgcc                                                                       <  1:3403636/101‑4 (MQ=255)
                             tCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTcgcc                                                                       <  1:4144321/101‑4 (MQ=255)
                              cGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTcgcca                                                                      >  1:1142778/1‑97 (MQ=255)
                                aggCGCCCGTGGCCTTCTTTTTAGTGACCAACCCCAAGTCCTCGGCAGTGGCTGATCATGTCGGATTCGCGCTGGCGGCGAACAAGGCCGGTGTCGCCAAg                                                                    <  2:931447/99‑1 (MQ=255)
                                aTGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAg                                                                    <  2:1546986/101‑1 (MQ=255)
                                aTGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAg                                                                    >  1:17379/1‑101 (MQ=255)
                                aTGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAg                                                                    >  2:3597531/1‑101 (MQ=255)
                                aTGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAg                                                                    >  1:1897544/1‑101 (MQ=255)
                                 tGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGc                                                                   <  1:503107/101‑1 (MQ=255)
                                 tGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGc                                                                   <  1:58369/101‑1 (MQ=255)
                                     aCCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATgg                                                               >  2:1343746/1‑101 (MQ=255)
                                          ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACt                                                          <  1:3344606/101‑1 (MQ=255)
                                          ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACt                                                          <  2:3332053/101‑1 (MQ=255)
                                          ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACt                                                          <  1:1926524/101‑1 (MQ=255)
                                           gccgccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTg                                                         >  1:3821248/1‑101 (MQ=255)
                                             cgccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGc                                                       <  2:3015938/101‑1 (MQ=255)
                                             cgccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGc                                                       <  1:3683777/101‑1 (MQ=255)
                                               ccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGctt                                                     >  2:2497296/1‑100 (MQ=255)
                                               ccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGctc                                                     <  2:1780279/101‑1 (MQ=255)
                                               ccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGctc                                                     >  1:931493/1‑101 (MQ=255)
                                                  ctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTgg                                                  >  1:3068207/1‑101 (MQ=255)
                                                  ctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTgg                                                  >  2:242685/1‑101 (MQ=255)
                                                  ctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTgg                                                  <  1:4160584/101‑1 (MQ=255)
                                                    ttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGc                                                <  2:1033949/101‑1 (MQ=255)
                                                     tCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGcc                                               <  2:73795/101‑1 (MQ=255)
                                                      cGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCt                                              <  2:1867090/101‑1 (MQ=255)
                                                        tGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTgg                                            <  1:657531/101‑1 (MQ=255)
                                                        tGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTgg                                            >  2:2722377/1‑101 (MQ=255)
                                                         gACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGa                                           <  2:2779884/101‑1 (MQ=255)
                                                          aCCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGaa                                          >  1:1403664/1‑101 (MQ=255)
                                                            cAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcc                                        <  2:906391/101‑1 (MQ=255)
                                                             aaCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcct                                       >  2:1690069/1‑100 (MQ=255)
                                                             aaCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcct                                       <  2:3067982/101‑2 (MQ=255)
                                                              aCCCCAAGGCCTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctc                                      <  2:81461/101‑3 (MQ=255)
                                                              aCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctc                                      <  2:634000/101‑3 (MQ=255)
                                                               ccccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctcg                                     >  2:3715006/1‑98 (MQ=255)
                                                               ccccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctcg                                     >  1:227309/1‑98 (MQ=255)
                                                                   aaGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCat                                >  2:39206/1‑100 (MQ=255)
                                                                   aaGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCat                                >  2:1198791/1‑100 (MQ=255)
                                                                    aGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCatt                               <  1:3534911/101‑3 (MQ=255)
                                                                     ggACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattc                              <  2:3370631/101‑4 (MQ=255)
                                                                         tCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggc                         >  2:1687423/1‑101 (MQ=255)
                                                                         tCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggc                         >  2:845581/1‑101 (MQ=255)
                                                                         tCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggc                         >  1:2413043/1‑101 (MQ=255)
                                                                          cGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggcg                        <  1:2632187/101‑1 (MQ=255)
                                                                               gTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCAc                   >  1:1018891/1‑101 (MQ=255)
                                                                               gTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCAc                   >  1:702758/1‑101 (MQ=255)
                                                                                 ggCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGc                 >  1:2008488/1‑101 (MQ=255)
                                                                                  gCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCa                <  1:2798514/101‑1 (MQ=255)
                                                                                   ccGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaa               <  2:4228651/101‑1 (MQ=255)
                                                                                    cGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaa              >  1:274003/1‑101 (MQ=255)
                                                                                    cGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaa              >  1:1172239/1‑101 (MQ=255)
                                                                                    cGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaa              >  1:3091030/1‑101 (MQ=255)
                                                                                     gATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaa             >  2:1247742/1‑101 (MQ=255)
                                                                                       tCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCCCGCaaaagg           >  2:3398835/1‑99 (MQ=255)
                                                                                       tCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaagg           >  1:429767/1‑99 (MQ=255)
                                                                                        cATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggc          <  2:2302291/101‑4 (MQ=255)
                                                                                        cATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggc          <  2:1263129/101‑4 (MQ=255)
                                                                                           gTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGa      >  1:435007/1‑101 (MQ=255)
                                                                                              ggcttcgcgcTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc   <  1:741644/101‑1 (MQ=255)
                                                                                              ggcttcgcgcTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc   >  2:3138241/1‑101 (MQ=255)
                                                                                               gcttcgcgcTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcg  <  1:1690184/101‑1 (MQ=255)
                                                                                               gcttcgcgcTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcg  <  2:2094320/101‑1 (MQ=255)
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CTTCCAGCAGGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCTGGC.CCGAACAAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCG  >  NC_014034/2180061‑2180256

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG