Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,240,244 +C 98.2% intergenic (+39/+139) RCAP_rcc02072 → / ← RCAP_rct00029 nonfunctional major facilitator superfamily protein/tRNA‑Gly

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,240,2441.→C98.2% 4.1 ‑0.5 ‑1.1221intergenic (+39/+139)RCAP_rcc02072/RCAP_rct00029nonfunctional major facilitator superfamily protein/tRNA‑Gly
Reads supporting (aligned to +/- strand):  new base (104/113):  ref base (1/3):  total (105/116)
Fisher's exact test strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.44e-02

Only 100 of 244 total aligned reads displayed.

GGCGTGATACTGAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.A.CCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGAAGGGACGCAA  >  NC_014034/2240144‑2240339
                                                                                                      |                                                                                               
ggCGTGATACTGAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGa.a                                                                                                  >  2:3720021/1‑101 (MQ=255)
  cGTGATCCTGAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.A.cc                                                                                               <  1:703832/101‑1 (MQ=255)
  cGTGATACTGAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.A.cc                                                                                               >  2:733344/1‑101 (MQ=255)
    tGATACTGAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.A.ccct                                                                                             <  2:2354627/101‑3 (MQ=255)
     gATACTGAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.A.ccctg                                                                                            >  1:3835674/1‑98 (MQ=255)
     gATACTGAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.A.ccctg                                                                                            >  1:886548/1‑98 (MQ=255)
     gATACTGAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCCTGGGGATGACCTCGGAAACGTCCTTGGGA.A.ccctg                                                                                            >  2:1332209/1‑98 (MQ=255)
         cTGAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTgcggc                                                                                         <  1:2896871/101‑1 (MQ=255)
         cTGAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTgcggc                                                                                         >  2:432345/1‑101 (MQ=255)
          tGAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTgcggcg                                                                                        >  1:160674/1‑101 (MQ=255)
          tGAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTgcggcg                                                                                        >  1:1109211/1‑101 (MQ=255)
            aGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGt                                                                                      >  1:1143927/1‑101 (MQ=255)
             ggACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTa                                                                                     >  2:3624136/1‑101 (MQ=255)
              gACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTAt                                                                                    >  1:937491/1‑101 (MQ=255)
              gACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTAt                                                                                    <  2:458450/101‑1 (MQ=255)
              gACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTAt                                                                                    <  2:747835/101‑1 (MQ=255)
               aCAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTAtt                                                                                   <  1:837556/101‑1 (MQ=255)
               aCAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTAtt                                                                                   <  2:2691713/101‑1 (MQ=255)
               aCAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTAtt                                                                                   <  2:2029820/101‑1 (MQ=255)
               aCAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTAtt                                                                                   >  2:1548944/1‑101 (MQ=255)
                cAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTg                                                                                  <  1:2346042/101‑1 (MQ=255)
                  ggcggcAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGcc                                                                                >  1:1369854/1‑101 (MQ=255)
                    cggcAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCgg                                                                              <  1:1036381/101‑1 (MQ=255)
                      gcAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCt                                                                            <  2:1699150/101‑1 (MQ=255)
                      gcAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCt                                                                            >  2:1124234/1‑101 (MQ=255)
                        aaGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTc                                                                          >  1:2989497/1‑101 (MQ=255)
                        aaGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTc                                                                          <  1:3080922/101‑1 (MQ=255)
                        aaGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTc                                                                          >  1:2920830/1‑101 (MQ=255)
                         tgggCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTcc                                                                         <  2:1184334/100‑1 (MQ=255)
                          gggCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTccc                                                                        >  2:4160015/1‑101 (MQ=255)
                           ggCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCg                                                                       <  1:647459/101‑1 (MQ=255)
                           ggCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACCCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCg                                                                       <  1:3624389/101‑1 (MQ=255)
                              ttccttcTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGacc                                                                    >  2:178347/1‑99 (MQ=255)
                              ttccttcTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATc                                                                    >  2:893561/1‑101 (MQ=255)
                               tccttcTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGATC.CCTGCGGCGGTATTGCCGGCTTCCCGATCt                                                                   <  2:2784116/101‑1 (MQ=255)
                               tccttcTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCt                                                                   <  2:2881911/101‑1 (MQ=255)
                               tccttcTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCt                                                                   <  1:597221/101‑1 (MQ=255)
                                ccttcTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTg                                                                  <  2:942468/101‑1 (MQ=255)
                                 cttcTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTGTTGCCGGCTTCCCGATCTgg                                                                 <  2:328634/101‑1 (MQ=255)
                                 cttcTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTgg                                                                 <  2:1212071/101‑1 (MQ=255)
                                 cttcTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTgg                                                                 <  1:3559444/101‑1 (MQ=255)
                                  ttcTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGt                                                                <  2:2291175/101‑1 (MQ=255)
                                   tcTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGtt                                                               >  1:1136635/1‑101 (MQ=255)
                                   tcTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGtt                                                               >  1:1450711/1‑101 (MQ=255)
                                   tcTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGtt                                                               >  1:1847538/1‑101 (MQ=255)
                                    cTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGttt                                                              <  1:2736083/101‑1 (MQ=255)
                                    cTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGttt                                                              >  1:3285780/1‑101 (MQ=255)
                                    cTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGttt                                                              <  1:476936/101‑1 (MQ=255)
                                     tGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTg                                                             <  1:2121705/101‑1 (MQ=255)
                                       ggAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCt                                                           <  1:3446831/101‑1 (MQ=255)
                                             gCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGt                                                     <  2:2989275/101‑1 (MQ=255)
                                             gCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGt                                                     >  2:276046/1‑101 (MQ=255)
                                             gCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGt                                                     >  2:2250218/1‑101 (MQ=255)
                                              cccGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTg                                                    <  2:1615387/101‑1 (MQ=255)
                                               ccGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGc                                                   <  1:2940492/101‑1 (MQ=255)
                                               ccGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGGATTGCCGGCTTCCCGATCTGGGTTGCTCAACGTgg                                                   >  2:3992242/1‑100 (MQ=38)
                                                cGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCt                                                  >  2:147725/1‑101 (MQ=255)
                                                cGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCt                                                  <  2:600056/101‑1 (MQ=255)
                                                  gCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTc                                                <  1:990120/101‑1 (MQ=255)
                                                  gCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTc                                                <  2:3522465/101‑1 (MQ=255)
                                                    ttGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCaa                                              <  2:819962/101‑1 (MQ=255)
                                                    ttGAGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCaa                                              >  2:267257/1‑101 (MQ=255)
                                                       cgcTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGAc                                           <  2:1092117/101‑1 (MQ=255)
                                                       cgcTTGAACTCGCGCTTGGGGATGACCTCGGAAAAGTCCTTGGGA.ACCCTGCGGCGGCATTGCCGGCTTTCCGATCTGGTTTGCTCAACGTGCTTCAAGAc                                           >  1:4200936/1‑101 (MQ=38)
                                                         cTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACaa                                         <  1:2779584/101‑1 (MQ=255)
                                                          ttGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAg                                        <  2:184646/101‑1 (MQ=255)
                                                           tGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGc                                       >  2:504878/1‑101 (MQ=255)
                                                            gAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGcc                                      >  1:1504254/1‑101 (MQ=255)
                                                             aaCTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGccc                                     >  2:1513237/1‑101 (MQ=255)
                                                                tCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGt                                  >  1:3123385/1‑101 (MQ=255)
                                                                tCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGt                                  >  1:4095642/1‑101 (MQ=255)
                                                                 cgcgCTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTa                                 >  2:49009/1‑101 (MQ=255)
                                                                 cgcgCTTGGGGATGACCNCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTa                                 <  2:1118328/101‑1 (MQ=255)
                                                                  gcgcTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTAt                                >  1:3739656/1‑101 (MQ=255)
                                                                  gcgcTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTAt                                >  2:657642/1‑101 (MQ=255)
                                                                    gcTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTAtgt                              >  1:2046286/1‑101 (MQ=255)
                                                                    gcTTGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTAtgt                              >  2:1669551/1‑101 (MQ=255)
                                                                      ttGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGc                            >  2:3744134/1‑101 (MQ=255)
                                                                       tGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCt                           <  1:1747324/101‑1 (MQ=255)
                                                                       tGGGGATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCt                           >  2:3267017/1‑101 (MQ=255)
                                                                        ggggATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTc                          <  2:1362562/101‑1 (MQ=255)
                                                                         gggATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCg                         <  2:1318602/101‑1 (MQ=255)
                                                                          ggATGCCCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGa                        <  2:3852404/101‑1 (MQ=255)
                                                                          ggATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGa                        >  2:2375024/1‑101 (MQ=255)
                                                                           gATGACCTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGaa                       >  2:1398405/1‑101 (MQ=255)
                                                                            tccgatctggAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAAt                      <  1:443054/93‑1 (MQ=255)
                                                                                ccTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGc                  >  2:3248178/1‑101 (MQ=255)
                                                                                ccTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGc                  <  2:3086509/101‑1 (MQ=255)
                                                                                ccTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGc                  <  1:3153956/101‑1 (MQ=255)
                                                                                 cTCGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCa                 >  2:207910/1‑101 (MQ=255)
                                                                                   cGGAAACGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGAGAAGCCCCGTATGTGCTCGAATGTGCAcc               >  1:2530626/1‑101 (MQ=255)
                                                                                      aaaCGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGa            <  1:202021/101‑1 (MQ=255)
                                                                                       aaCGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGaa           <  2:1930325/101‑1 (MQ=255)
                                                                                        aCGTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGAAg          >  1:734470/1‑101 (MQ=255)
                                                                                          gTCCTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGAAggg        >  1:1973147/1‑101 (MQ=255)
                                                                                            ccTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGAAGGGAc      <  2:160636/101‑1 (MQ=255)
                                                                                            ccTTGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGAAGGGAc      >  2:3385345/1‑101 (MQ=255)
                                                                                              ttGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGAAGGGACGc    >  2:2201555/1‑101 (MQ=255)
                                                                                              ttGGGA.ACCCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGAAGGGACGc    <  1:2260169/101‑1 (MQ=255)
                                                                                                gggA.ACCCTGCGGCGGTATTGCCGGCTTCCGGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGAAGGGACGCaa  <  1:267302/101‑1 (MQ=255)
                                                                                                      |                                                                                               
GGCGTGATACTGAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTTGGGA.A.CCTGCGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGAAGGGACGCAA  >  NC_014034/2240144‑2240339

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG