Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,300 +C 2.1% intergenic (‑96/‑209) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3001.→C 2.1% 5.1 ‑2.1 ‑1.9193intergenic (‑96/‑209)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (98/91):  total (102/91)
Fisher's exact test strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.20e-02

Only 100 of 211 total aligned reads displayed.

CATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.C.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCTTCG  >  NC_014034/2249202‑2249400
                                                                                                     |                                                                                                       
cATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.c                                                                                                        >  1:1042771/1‑101 (MQ=255)
cATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.c                                                                                                        <  2:3571529/101‑1 (MQ=255)
  tGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.ccc                                                                                                      >  2:1422282/1‑101 (MQ=255)
   ggTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.cccc                                                                                                     >  1:2542078/1‑101 (MQ=255)
    gTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.ccccc                                                                                                    >  2:1899293/1‑101 (MQ=255)
    gTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.ccccc                                                                                                    <  1:2870309/101‑1 (MQ=255)
     tGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.TCCCCg                                                                                                   >  2:1693482/1‑101 (MQ=255)
       cTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCggg                                                                                                 >  2:559888/1‑101 (MQ=255)
        tCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGa                                                                                                >  2:1196231/1‑101 (MQ=255)
        tCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGa                                                                                                <  1:3927468/101‑1 (MQ=255)
         tccgatctttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGat                                                                                               <  2:2682214/93‑1 (MQ=255)
         cGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGat                                                                                               >  1:3593273/1‑101 (MQ=255)
          gCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGata                                                                                              >  2:4207488/1‑101 (MQ=255)
          gCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGata                                                                                              >  1:2682864/1‑101 (MQ=255)
           cTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGatat                                                                                             <  2:1004353/101‑1 (MQ=255)
            tttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATAtt                                                                                            <  1:1908162/101‑1 (MQ=255)
            tttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATAtt                                                                                            >  1:1997956/1‑101 (MQ=255)
             ttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATAttt                                                                                           >  1:809752/1‑101 (MQ=255)
             ttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATAttt                                                                                           <  2:18237/101‑1 (MQ=255)
              tCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTg                                                                                          >  2:3132011/1‑101 (MQ=255)
              tCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTg                                                                                          <  1:3997773/101‑1 (MQ=255)
              tCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTg                                                                                          <  2:4031236/101‑1 (MQ=255)
               cATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGa                                                                                         >  1:3152663/1‑101 (MQ=255)
                 ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCCCCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAgg                                                                                       <  2:4013850/101‑1 (MQ=255)
                 ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAgg                                                                                       <  2:3437479/101‑1 (MQ=255)
                  tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGc                                                                                      >  1:1818021/1‑101 (MQ=255)
                   ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCa                                                                                     >  1:899736/1‑101 (MQ=255)
                    gtgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCaa                                                                                    <  2:3036540/101‑1 (MQ=255)
                     tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAg                                                                                   <  2:1091656/101‑1 (MQ=255)
                       ttCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGAt                                                                                 <  2:1988957/101‑1 (MQ=255)
                       ttCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGAt                                                                                 <  2:914546/101‑1 (MQ=255)
                        tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATg                                                                                >  1:2916977/1‑101 (MQ=255)
                        tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATg                                                                                <  1:1123387/101‑1 (MQ=255)
                         cTTGAGATGCACAGATACCGCAGCTTGGGCACGCGGGGCAAGAAGGGGCCTCGG.CTCCCGCCCCGTGCCTGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                               <  1:1515556/101‑1 (MQ=38)
                          ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGaa                                                                              >  2:87558/1‑101 (MQ=255)
                            gagaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gg                                                                         <  2:2171807/101‑1 (MQ=255)
                             agaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...ggg                                                                        >  1:472561/1‑101 (MQ=255)
                             agaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...ggg                                                                        <  1:1196325/101‑1 (MQ=255)
                                  cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCt                                                                   <  2:90819/101‑1 (MQ=255)
                                    caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCttt                                                                 >  2:2580528/1‑101 (MQ=255)
                                      gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCa                                                               >  1:136121/1‑101 (MQ=255)
                                      gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCa                                                               <  1:82422/101‑1 (MQ=255)
                                       aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCAt                                                              >  1:2302663/1‑101 (MQ=255)
                                        tACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATc                                                             <  2:2965172/101‑1 (MQ=255)
                                         aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCt                                                            <  2:3361288/101‑1 (MQ=255)
                                         aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCt                                                            >  2:484617/1‑101 (MQ=255)
                                         aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCt                                                            <  2:2541887/101‑1 (MQ=255)
                                            gCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGc                                                         <  2:34017/101‑1 (MQ=255)
                                               gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGa                                                      >  2:329499/1‑101 (MQ=255)
                                                tttGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaa                                                     <  1:3389755/100‑1 (MQ=255)
                                                cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaa                                                     <  1:2138596/101‑1 (MQ=255)
                                                cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaa                                                     <  1:2730742/101‑1 (MQ=255)
                                                  tGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaaaa                                                   >  1:1777245/1‑101 (MQ=255)
                                                   gggGACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                  <  1:3101496/101‑1 (MQ=255)
                                                   gggCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                  <  1:1160251/101‑1 (MQ=255)
                                                   gggCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                  >  1:1706438/1‑101 (MQ=255)
                                                     gCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAatat                                                <  2:1046824/101‑1 (MQ=255)
                                                      cACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATc                                               <  1:137626/101‑1 (MQ=255)
                                                        cccGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGTA...GGGGGGCTTTCATCTTGCCGAAAATATccc                                             <  1:3762461/101‑1 (MQ=255)
                                                        cccGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATccc                                             <  2:1371457/101‑1 (MQ=255)
                                                          cGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgg                                           >  2:3718310/1‑101 (MQ=255)
                                                          cGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgg                                           >  2:2229856/1‑101 (MQ=255)
                                                           ggggCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCggg                                          >  1:1677672/1‑101 (MQ=255)
                                                              gCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgggggg                                       <  2:1527196/101‑1 (MQ=255)
                                                               cAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCggggggg                                      >  1:24659/1‑100 (MQ=255)
                                                               cAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                      >  1:602512/1‑101 (MQ=255)
                                                                aagaagGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                     <  2:3329656/101‑1 (MQ=255)
                                                                  gaagGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                   >  2:3752254/1‑101 (MQ=255)
                                                                    ggggggCTCTGCC.CCCGCCCCGTGCCGGGCCT.CTGCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATg                                 <  1:3633811/100‑1 (MQ=37)
                                                                    agGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATg                                 >  2:1251327/1‑101 (MQ=255)
                                                                    agGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATg                                 <  1:2616160/101‑1 (MQ=255)
                                                                     gggggCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCACGGGGGGTGAATgg                                >  1:450802/1‑101 (MQ=255)
                                                                      ggggCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                               <  1:2230015/101‑1 (MQ=255)
                                                                       gggCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcg                              >  2:208618/1‑101 (MQ=255)
                                                                       gggCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcg                              <  1:2264758/101‑1 (MQ=255)
                                                                        ggCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcgc                             <  2:1721042/101‑1 (MQ=255)
                                                                          ctctGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAg                           <  1:1238753/101‑1 (MQ=255)
                                                                           tctgcc.cccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGc                          >  1:4224026/6‑101 (MQ=255)
                                                                             tgcc.cccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCa                        >  1:3225845/4‑101 (MQ=255)
                                                                                c.cccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCagag                     >  2:2916409/1‑101 (MQ=255)
                                                                                  cccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgg                    >  1:4129767/1‑101 (MQ=255)
                                                                                   ccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAggg                   <  2:2269323/101‑1 (MQ=255)
                                                                                    cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgggg                  >  2:3683041/1‑101 (MQ=255)
                                                                                        ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCa              <  1:560713/101‑1 (MQ=255)
                                                                                        ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCa              <  2:436042/101‑1 (MQ=255)
                                                                                         cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg             <  2:24645/101‑1 (MQ=255)
                                                                                         cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg             >  1:84139/1‑101 (MQ=255)
                                                                                          gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgc            >  2:1123088/1‑101 (MQ=255)
                                                                                          gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgc            <  1:1001720/101‑1 (MQ=255)
                                                                                          gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAgggggg                >  2:4122634/7‑101 (MQ=21)
                                                                                          gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAgggggg                >  1:144992/7‑101 (MQ=18)
                                                                                            gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgcgc          >  1:3537406/1‑101 (MQ=255)
                                                                                              cGGGACT.CCTCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGccc        <  2:459552/101‑1 (MQ=255)
                                                                                              cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGccc        >  2:2919430/1‑101 (MQ=255)
                                                                                              cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGccc        >  1:995632/1‑101 (MQ=255)
                                                                                              cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGccc        >  1:2850463/1‑101 (MQ=255)
                                                                                               gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGcccc       >  1:1616232/1‑101 (MQ=255)
                                                                                                 gACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGcccccc     <  2:3593020/101‑2 (MQ=255)
                                                                                                   cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGcccccctt   >  1:3144277/1‑98 (MQ=255)
                                                                                                    t.CCCCCGAGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGccccccttc  >  2:1624239/1‑97 (MQ=255)
                                                                                                     |                                                                                                       
CATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.C.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCTTCG  >  NC_014034/2249202‑2249400

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG