Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,331 +A 2.1% intergenic (‑127/‑178) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3312.→A 2.1% 8.4 ‑2.1 ‑0.5187intergenic (‑127/‑178)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (93/90):  total (97/90)
Fisher's exact test strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.26e-01

Only 100 of 205 total aligned reads displayed.

GATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAG  >  NC_014034/2249232‑2249428
                                                                                                         |                                                                                                        
gaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg                                                                                                           >  2:2918460/1‑101 (MQ=255)
gaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg                                                                                                           <  2:104059/101‑1 (MQ=255)
 aTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg..g                                                                                                        >  2:1160157/1‑101 (MQ=255)
      caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCttt                                                                                                   >  2:2580528/1‑101 (MQ=255)
      caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCttt                                                                                                   >  2:2944183/1‑101 (MQ=255)
       aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTc                                                                                                  <  2:3572785/101‑1 (MQ=255)
        gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCa                                                                                                 <  1:82422/101‑1 (MQ=255)
        gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCa                                                                                                 <  2:3723645/101‑1 (MQ=255)
          tACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATc                                                                                               <  1:629049/101‑1 (MQ=255)
           aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCt                                                                                              <  2:2541887/101‑1 (MQ=255)
           aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCt                                                                                              >  2:484617/1‑101 (MQ=255)
           aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCt                                                                                              <  2:3361288/101‑1 (MQ=255)
           aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATCAA...GGGG..GGCTTTCATCt                                                                                              >  1:289967/1‑101 (MQ=255)
            ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCtt                                                                                             <  1:2985079/101‑1 (MQ=255)
              gCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGc                                                                                           <  2:34017/101‑1 (MQ=255)
                 gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAC...GGGG..GGCTTTCATCTTGCCGa                                                                                        <  1:2398280/101‑1 (MQ=255)
                 gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGa                                                                                        >  2:329499/1‑101 (MQ=255)
                  tttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaa                                                                                       <  1:3389755/100‑1 (MQ=255)
                  cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaa                                                                                       <  1:2730742/101‑1 (MQ=255)
                   ttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaa                                                                                      <  2:641662/101‑1 (MQ=255)
                    tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaaa                                                                                     >  2:1461011/1‑101 (MQ=255)
                    tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaaa                                                                                     >  1:1777245/1‑101 (MQ=255)
                     gggGACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                    <  1:3101496/101‑1 (MQ=255)
                     gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                    <  1:1160251/101‑1 (MQ=255)
                     gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                    <  1:2869083/101‑1 (MQ=255)
                        cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                                 <  2:3329254/101‑1 (MQ=255)
                        cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                                 <  1:4028292/101‑1 (MQ=255)
                        cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                                 <  1:137626/101‑1 (MQ=255)
                        cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                                 <  1:1510934/101‑1 (MQ=255)
                          cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGTA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                               <  1:3762461/101‑1 (MQ=255)
                          cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                               >  2:1547873/1‑101 (MQ=255)
                          cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                               <  1:87582/101‑1 (MQ=255)
                            cGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgg                                                                             >  2:2229856/1‑101 (MQ=255)
                                 cAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggggggg                                                                        >  2:1859467/1‑100 (MQ=255)
                                 cAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggggggg                                                                        >  1:24659/1‑100 (MQ=255)
                                 cAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                                                        >  1:480985/1‑101 (MQ=255)
                                  aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                       <  2:3511322/101‑1 (MQ=255)
                                    gaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                     <  2:911974/101‑1 (MQ=255)
                                      ggggggCTCTGCCCCCGCCCCGTGCCGGGCCT.CTGCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                  <  1:3633811/100‑1 (MQ=37)
                                      agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                  <  1:2616160/101‑1 (MQ=255)
                                       gggggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCACGGGGGGTGAA.Tgg                                                                 >  1:450802/1‑101 (MQ=255)
                                         gggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc.g                                                              <  2:1312539/101‑1 (MQ=255)
                                         gggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc.g                                                              >  2:208618/1‑101 (MQ=255)
                                         gggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc.g                                                              >  1:2206728/1‑101 (MQ=255)
                                         gggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc.g                                                              <  1:2264758/101‑1 (MQ=255)
                                           gCTCTGCCCCCGTCCCTTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCa                                                            <  2:1105257/101‑1 (MQ=255)
                                            ctctGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..g                                                         <  1:106933/101‑1 (MQ=255)
                                            ctctGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..g                                                         <  1:1238753/101‑1 (MQ=255)
                                             tctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..Gc                                                        >  1:4224026/6‑101 (MQ=255)
                                             tctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGC.GCA..Gc                                                        >  1:811589/6‑101 (MQ=255)
                                              ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..Gcc                                                       <  1:1853884/97‑1 (MQ=255)
                                                gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCag                                                     >  1:3162421/3‑101 (MQ=255)
                                                gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCag                                                     >  2:1915529/3‑101 (MQ=255)
                                                gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGC.GCA..GCCag                                                     >  1:2243123/3‑101 (MQ=255)
                                                  ccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCagag                                                   >  2:2916409/1‑101 (MQ=255)
                                                  ccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..CCCagag                                                   >  2:1681540/1‑101 (MQ=255)
                                                   cccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAg.g                                                 <  1:368489/101‑1 (MQ=255)
                                                     cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAg.ggg                                               >  2:3683041/1‑101 (MQ=255)
                                                     cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAg.ggg                                               >  1:833390/1‑101 (MQ=255)
                                                        ccgatctcGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGc                                            <  2:425147/94‑1 (MQ=255)
                                                        cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGc                                            >  2:3872364/1‑101 (MQ=255)
                                                          cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAg                                          <  2:24645/101‑1 (MQ=255)
                                                          cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC.GAA..GCCAGAg.gggg                                              >  2:2990221/8‑101 (MQ=18)
                                                           gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAgc                                         >  2:4053323/1‑101 (MQ=255)
                                                           gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAgc                                         <  1:1001720/101‑1 (MQ=255)
                                                           gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAgc                                         <  2:1711979/101‑1 (MQ=255)
                                                           gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAgc                                         >  2:1123088/1‑101 (MQ=255)
                                                           gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC.GAA..GCCAGAg.ggggg                                             >  1:144992/7‑101 (MQ=18)
                                                             gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAgcg.c                                      >  1:3537406/1‑101 (MQ=255)
                                                               cGGGACT.CCTCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCG.ccc                                    <  2:459552/101‑1 (MQ=255)
                                                               cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAgatcgg                                     >  1:425204/1‑96 (MQ=255)
                                                               cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCG.ccc                                    >  1:1918090/1‑101 (MQ=255)
                                                                gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCG.cccc                                   >  1:1616232/1‑101 (MQ=255)
                                                                 ggACT.CCCCCGGGATATTTGAGGCAAGATGAC...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCG.ccccc                                  >  1:3808942/1‑101 (MQ=255)
                                                                   aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.TGGGGCAGCG.cccccct                                <  2:4129505/101‑3 (MQ=255)
                                                                    cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCG.cccccctt                               >  1:3144277/1‑98 (MQ=255)
                                                                     t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCG.ccccccttc                              >  1:1710117/1‑97 (MQ=255)
                                                                     t.CCCCCGAGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCG.ccccccttc                              >  2:1624239/1‑97 (MQ=255)
                                                                       cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCG.ccccccttcg                             <  2:3959378/101‑6 (MQ=255)
                                                                       cccccGGGATATTTGAGGCAAGAGGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCG.ccccccttcg                             <  2:450745/101‑6 (MQ=255)
                                                                        ccccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGC.CAAAGGCCAAGA.GGGGGCAGCG.CCCCCtg                                >  2:1635175/1‑100 (MQ=0)
                                                                        ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTcgc                             >  1:2354331/1‑101 (MQ=255)
                                                                         cccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTcgcg                            <  2:3190971/101‑1 (MQ=255)
                                                                         cccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAAGATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTcgcg                            >  1:3579042/1‑101 (MQ=255)
                                                                           cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGC.CAAAGGCCAAGA.GGGGGCAGCG.CCCCCtgccc                             >  2:1996119/1‑97 (MQ=2)
                                                                            gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCgcgggc                         <  2:410638/101‑1 (MQ=255)
                                                                            gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCgcgggc                         >  2:3783320/1‑101 (MQ=255)
                                                                             ggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCgcgtgcg                        >  1:1361193/1‑101 (MQ=255)
                                                                                tatTTGCGGAAAGAGGAA...GGGGCATGTTTTCATCTTGCCCAAAATATCCC.GGGGGTGAATTGGCAAAA..GGCAAAGAGGGGGCAGCG.CCCCCtgccccgccg                        <  2:3139117/101‑10 (MQ=9)
                                                                                 atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTCCGCGGGCGGCcg                    >  1:991321/1‑101 (MQ=255)
                                                                                   ttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGGG.GGGGGCAGCGCCCCCCTTCGCGGCCGGCcgcg                  >  2:1268224/1‑101 (MQ=37)
                                                                                       ggCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAcgc              >  2:370932/1‑101 (MQ=255)
                                                                                        gCAAGGTGGA...GGGG..GGCTCTCATCGTGCCGAAAATGCCCCGGGGGGTGAA.TGGC.GCA..GCCAGGG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAcgcg             <  1:1681655/101‑1 (MQ=25)
                                                                                             aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGcgccg        >  2:3618452/1‑101 (MQ=255)
                                                                                               gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa      >  1:735256/1‑101 (MQ=255)
                                                                                                aa...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCaa     >  2:2817288/1‑101 (MQ=255)
                                                                                                 a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc    <  2:2262820/101‑1 (MQ=255)
                                                                                                 a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc    <  2:1817901/101‑1 (MQ=255)
                                                                                                     gggg..ggTTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa   <  2:899651/101‑1 (MQ=255)
                                                                                                      ggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCCACAg  >  2:1053412/1‑101 (MQ=255)
                                                                                                         |                                                                                                        
GATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAG  >  NC_014034/2249232‑2249428

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG