Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,367 +T 1.7% intergenic (‑163/‑142) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3671.→T 1.7% 4.5 ‑1.4 ‑0.8177intergenic (‑163/‑142)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (87/87):  total (90/87)
Fisher's exact test strand distribution p-value = 2.46e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.54e-01

Only 100 of 199 total aligned reads displayed.

GAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CG.C.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATA  >  NC_014034/2249268‑2249466
                                                                                                            |                                                                                                         
gaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGaa                                                                                                            <  2:911974/101‑1 (MQ=255)
gaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGaa                                                                                                            >  2:3752254/1‑101 (MQ=255)
 aagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.t                                                                                                          >  2:954070/1‑101 (MQ=255)
  agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.Tg                                                                                                         >  2:1251327/1‑101 (MQ=255)
     gggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGgcg                                                                                                      <  1:2264758/101‑1 (MQ=255)
     gggCTCTGCCCCCGTCCCGTGACGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGgcg                                                                                                      >  2:1753689/1‑101 (MQ=255)
       gCTCTGCCCCCGTCCCTTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCa                                                                                                    <  2:1105257/101‑1 (MQ=255)
        ctctGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..g                                                                                                 <  1:106933/101‑1 (MQ=255)
        ctctGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..g                                                                                                 <  1:1238753/101‑1 (MQ=255)
          ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..Gcc                                                                                               <  1:1853884/97‑1 (MQ=255)
            gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCag                                                                                             >  2:1915529/3‑101 (MQ=255)
            gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGGGAA.TGGCGCA..GCCag                                                                                             >  1:2243123/3‑101 (MQ=255)
               cccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAgg                                                                                          >  1:4129767/1‑101 (MQ=255)
                ccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAggg                                                                                         <  2:2269323/101‑1 (MQ=255)
                 cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAgggg                                                                                        >  2:3683041/1‑101 (MQ=255)
                 cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAgggg                                                                                        >  1:833390/1‑101 (MQ=255)
                    ccgatctcGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                                     <  2:425147/94‑1 (MQ=255)
                     ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                                                                    <  1:560713/101‑1 (MQ=255)
                      cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.g                                                                                  <  2:24645/101‑1 (MQ=255)
                      cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGCGAA..GCCAGAggggg                                                                                       >  2:2990221/8‑101 (MQ=18)
                       gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.g.c                                                                                <  2:1711979/101‑1 (MQ=255)
                       gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.g.c                                                                                >  2:1123088/1‑101 (MQ=255)
                       gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.g.c                                                                                >  2:4053323/1‑101 (MQ=255)
                       gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.g.c                                                                                <  1:1001720/101‑1 (MQ=255)
                        caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGCGAA..GCCAGAggggggg                                                                                     >  2:882955/6‑100 (MQ=18)
                         gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.g.cg.c                                                                             >  1:3537406/1‑101 (MQ=255)
                           cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CG.c.cc                                                                          >  1:995632/1‑101 (MQ=255)
                           cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CG.c.cc                                                                          >  1:1918090/1‑101 (MQ=255)
                           cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CG.c.cc                                                                          >  1:2850463/1‑101 (MQ=255)
                              gACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CG.c.ccccc                                                                       <  2:3593020/101‑2 (MQ=255)
                               aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGTGGGGCA.G.CG.c.ccccct                                                                      <  2:4129505/101‑3 (MQ=255)
                                 t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CG.c.cccccttc                                                                    >  1:1710117/1‑97 (MQ=255)
                                 t.CCCCCGAGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CG.c.cccccttc                                                                    >  2:1624239/1‑97 (MQ=255)
                                  ccccccGGGTTTTTTGGGGGAAGAGGAA...GGGG..GGTTTTCATTTTGCCAAAAAAATCCCGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CG.c.ccccctt                                                                     <  1:1422384/100‑4 (MQ=21)
                                  ccccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CG.c.cccccttc                                                                    <  2:2892488/100‑5 (MQ=255)
                                   cccccGGGATATTTGAGGCAAGAGGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CG.c.cccccttcg                                                                   <  2:450745/101‑6 (MQ=255)
                                    ccccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC..AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCA.G.CG.C.CCCCtg                                                                      >  2:1635175/1‑100 (MQ=0)
                                    ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTcgc                                                                   <  1:811842/101‑1 (MQ=255)
                                    ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTcgc                                                                   <  1:1547977/101‑1 (MQ=255)
                                     cccGGGATATTTGCAGCAAGGTGAAACCGGTT..GCCTTTCATCTGGCCCGAAATATCCC.GGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCA.G.CG.c.cctcctgc                                                                    <  1:291216/101‑7 (MQ=14)
                                     cccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTcgcg                                                                  <  2:3190971/101‑1 (MQ=255)
                                     cccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAAGATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTcgcg                                                                  >  1:3579042/1‑101 (MQ=255)
                                       cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGAGAAT.TGGCCAAAGGCCAAGAGGGGGCA.G.CG.C.CCCCtgacc                                                                   >  2:1879142/1‑97 (MQ=2)
                                       cGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCgcggg                                                                >  1:433227/1‑101 (MQ=255)
                                       cGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCgcggg                                                                >  1:2366116/1‑101 (MQ=255)
                                       cGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCgcggg                                                                <  2:3162182/101‑1 (MQ=255)
                                        gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCG.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCgcgggc                                                               <  2:2916758/101‑1 (MQ=255)
                                        gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCgcgggc                                                               >  1:1763632/1‑101 (MQ=255)
                                        gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCgcgggc                                                               <  2:410638/101‑1 (MQ=255)
                                            tatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGcc                                                           >  2:1689121/1‑101 (MQ=255)
                                             atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCcg                                                          >  1:3698587/1‑101 (MQ=255)
                                                 gAGGCAAGATGAA...GGGG..GTCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGAc                                                      <  2:1972989/101‑1 (MQ=255)
                                                  aGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGAcg                                                     >  2:3897094/1‑101 (MQ=255)
                                                   ggCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGAcgc                                                    >  1:910587/1‑101 (MQ=255)
                                                   ggCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGAcgc                                                    >  2:370932/1‑101 (MQ=255)
                                                      aaGAGGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGAcgcgcg                                                 <  1:2440432/101‑1 (MQ=255)
                                                       aGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACgcgcgc                                                >  1:4232831/1‑101 (MQ=255)
                                                          tGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgccgc                                             >  2:3799270/1‑101 (MQ=255)
                                                           gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa                                            >  1:735256/1‑101 (MQ=255)
                                                           gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa                                            <  2:3521903/101‑1 (MQ=255)
                                                           gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa                                            <  1:1461113/101‑1 (MQ=255)
                                                            aa...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCaa                                           >  2:2817288/1‑101 (MQ=255)
                                                             a...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                          <  1:1899413/101‑1 (MQ=255)
                                                             a...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                          <  2:1817901/101‑1 (MQ=255)
                                                                ggggg..ggTTTTCTTCTTGCGAAAAATTCCGG.GGGGGGTAAA.TGGCGCG..GCCGGGGGGGGGCG.GCCC.C.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                          <  1:3132242/100‑1 (MQ=255)
                                                                 gggg..ggCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa                                         >  2:1269296/1‑101 (MQ=255)
                                                                 gggg..ggCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGAGAA..GCCAGAGGGGGGCA.G.CG.CACGCCTTCGCGGGCGGGCGGGAAGCGcgccgccaaa                                         >  2:4008941/1‑97 (MQ=25)
                                                                  ggg..ggCTTTCATCTTGCCGAAAATATCCG.GGGGGGTGAA.TGGCTCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                                        <  1:382674/101‑1 (MQ=37)
                                                                  ggg..ggCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCCACAg                                        >  2:1053412/1‑101 (MQ=255)
                                                                       ggCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                                     <  2:2028296/101‑1 (MQ=255)
                                                                       ggCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                                     >  2:2568943/1‑101 (MQ=255)
                                                                        gCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCg                                    <  1:3207496/101‑1 (MQ=255)
                                                                        gCTTTCATCTTGCCGAAAATATCCC.GGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCg                                    <  1:3527858/101‑1 (MQ=255)
                                                                          tttCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                  >  1:1186225/1‑101 (MQ=255)
                                                                            tCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTTGTGcc                                >  2:2107527/1‑101 (MQ=255)
                                                                            tCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcc                                >  1:2272169/1‑101 (MQ=255)
                                                                             cATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                               <  1:2527817/101‑1 (MQ=255)
                                                                             cATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                               <  2:136097/101‑1 (MQ=255)
                                                                               tCTTGCCGAAAATATCCG.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCt                             <  1:2631767/101‑1 (MQ=255)
                                                                                 ttGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCGGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCTCCttt                           <  2:1677557/101‑1 (MQ=37)
                                                                                 ttGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                           <  1:1265657/101‑1 (MQ=255)
                                                                                  tGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTg                          >  1:1651336/1‑101 (MQ=255)
                                                                                  tGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTg                          >  1:2210826/1‑101 (MQ=255)
                                                                                  tGCCAAAAATATCCG.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTg                          <  2:2924812/101‑1 (MQ=38)
                                                                                   gCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGt                         >  2:3357811/1‑101 (MQ=255)
                                                                                    ccGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc                        >  1:3153813/1‑101 (MQ=255)
                                                                                       aaaaTATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGGGCCCCTTTGTCAtt                     >  1:1053140/1‑101 (MQ=255)
                                                                                        aaaTATCCC.GGGGGGTGAA.TGGCGCA..GCCAGGGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc                    <  1:3785762/101‑1 (MQ=255)
                                                                                                  ggggggTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAcc           <  1:208656/101‑1 (MQ=255)
                                                                                                    ggggTGAA.TGGCGCA..GCCAGAGGGGGCCACC.CC.C.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCa         <  2:514208/101‑1 (MQ=255)
                                                                                                     gggTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt        >  1:3355792/1‑101 (MQ=255)
                                                                                                      ggTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATg       <  2:3299626/101‑1 (MQ=255)
                                                                                                      ggTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATg       <  2:602448/101‑1 (MQ=255)
                                                                                                       gTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGt      >  2:148543/1‑101 (MQ=255)
                                                                                                       gTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGt      <  1:3028008/101‑1 (MQ=255)
                                                                                                       gTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGt      <  1:2610610/101‑1 (MQ=255)
                                                                                                        tGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTc     >  1:1124005/1‑101 (MQ=255)
                                                                                                         gAA.GGGCGCA..GCCAGGGGGGGGCA.GCCC.C.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa    <  1:3683295/101‑1 (MQ=255)
                                                                                                          aa.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt   >  1:779448/1‑101 (MQ=255)
                                                                                                           a.TGGCGCA..GCCAGAGGGGGGCA.G.CGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATa  <  1:3246538/101‑1 (MQ=255)
                                                                                                            |                                                                                                         
GAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.G.CG.C.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATA  >  NC_014034/2249268‑2249466

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG