Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,391 +C 94.4% intergenic (‑187/‑118) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3911.→C94.4% 17.4 ‑0.3 ‑0.2143intergenic (‑187/‑118)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (62/73):  ref base (3/5):  total (65/78)
Fisher's exact test strand distribution p-value = 7.28e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.80e-01

Only 100 of 174 total aligned reads displayed.

TGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCG.C.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTCC  >  NC_014034/2249291‑2249486
                                                                                                               |                                                                                                  
 gCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.gcg.c                                                                                                   >  1:3537406/1‑101 (MQ=255)
   cGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCG.c.c.c                                                                                               >  1:2850463/1‑101 (MQ=255)
   cGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCG.c.c.c                                                                                               >  1:995632/1‑101 (MQ=255)
    gggctccccccGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCA.gcg.c                                                                                                   <  1:3059470/95‑1 (MQ=17)
     ggACTCCCCCGGGATATTTGAGGCAAGATGAC...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCG.c.c.ccc                                                                                             >  1:3808942/1‑101 (MQ=255)
         tCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCG.c.c.ccccttc                                                                                         >  1:1710117/1‑97 (MQ=255)
         ccccccGGGTTTTTTGGGGGAAGAGGAA...GGGG..GGTTTTCATTTTGCCAAAAAAATCCCGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCG.c.c.cccctt                                                                                          <  1:1422384/100‑4 (MQ=21)
          cccccGGGATATTTGAGGCAAGAGGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCG.c.c.ccccttcg                                                                                        <  2:450745/101‑6 (MQ=255)
           ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.cgc                                                                                       <  1:1547977/101‑1 (MQ=255)
           ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.cgc                                                                                       <  2:1633285/101‑1 (MQ=255)
           ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.cgc                                                                                       >  1:2354331/1‑101 (MQ=255)
            cccGGGATATTTGCAGCAAGGTGAAACCGGTT..GCCTTTCATCTGGCCCGAAATATCCC.GGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCA.GCG.c.c.ctcctgc                                                                                         <  1:291216/101‑7 (MQ=14)
            cccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAAGATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.cgcg                                                                                      >  1:3579042/1‑101 (MQ=255)
             ccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.Cgcgg                                                                                     >  1:82437/1‑101 (MQ=255)
              cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGAGAAT.TGGCCAAAGGCCAAGAGGGGGCA.GCG.C.C.CCCtgacc                                                                                        >  2:1879142/1‑97 (MQ=2)
              cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC..AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCA.GCG.C.C.CCCtgccc                                                                                        >  2:1663196/1‑97 (MQ=0)
              cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC..AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCA.GCG.C.C.CCCtgccc                                                                                        >  2:1996119/1‑97 (MQ=2)
              cGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.Cgcggg                                                                                    >  1:636164/1‑101 (MQ=255)
              cGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.Cgcggg                                                                                    <  2:3162182/101‑1 (MQ=255)
               gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCG.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.Cgcgggc                                                                                   <  2:2916758/101‑1 (MQ=255)
               gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.Cgcgggc                                                                                   <  2:410638/101‑1 (MQ=255)
               gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.Cgcgggc                                                                                   >  1:1763632/1‑101 (MQ=255)
                ggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.Cgcgggcg                                                                                  <  2:2533023/101‑1 (MQ=255)
                    atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCcg                                                                              >  1:3698587/1‑101 (MQ=255)
                      ttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGGGGGGGGCA.GCGCC.C.CCCTT.CGCGGCCGGCcgcg                                                                            >  2:1268224/1‑101 (MQ=37)
                         aGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGAcg                                                                         >  2:3897094/1‑101 (MQ=255)
                          ggCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGAcgc                                                                        >  1:910587/1‑101 (MQ=255)
                            cAAGATGAA...GGGG..GGCTTTCATCTTGCCGAGAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGAcgcgc                                                                      >  1:3765924/1‑101 (MQ=255)
                             aaGATGAG...GGGG..GGCTTTCATCTTGCCGAAAATAGCGC.GGGGGGTGAA.TGGCGCA..GCGAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGAcgcgcg                                                                     <  2:1066977/101‑1 (MQ=37)
                             aaGAGGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGAcgcgcg                                                                     <  1:2440432/101‑1 (MQ=255)
                              aGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACgcgcgc                                                                    >  2:1588134/1‑101 (MQ=255)
                              aGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACgcgcgc                                                                    >  1:4232831/1‑101 (MQ=255)
                                aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGcgccg                                                                  >  2:3618452/1‑101 (MQ=255)
                                 tGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGcgccgc                                                                 >  2:3799270/1‑101 (MQ=255)
                                    a...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAAc                                                              <  2:1817901/101‑1 (MQ=255)
                                        gggg..ggCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGAGAA..GCCAGAGGGGGGCA.GCG.CAC.GCCTT.CGCGGGCGGGCGGGAAGCGcgccgccaaa                                                             >  2:4008941/1‑97 (MQ=25)
                                         ggg..ggCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCCACAg                                                            >  2:1053412/1‑101 (MQ=255)
                                                 tttCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                                      >  1:1186225/1‑101 (MQ=255)
                                                 tttCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                                      <  1:1915655/101‑1 (MQ=255)
                                                  ttCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGAGCA..GCCAGAGGGGGGCA.GCG.C.CACCCTC.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGGCGTGc                                                     >  1:1510896/1‑101 (MQ=37)
                                                   tCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTTGTGcc                                                    >  2:2107527/1‑101 (MQ=255)
                                                    cATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                                                   <  2:136097/101‑1 (MQ=255)
                                                    cATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                                                   <  2:3179523/101‑1 (MQ=255)
                                                      tCTTGCCGAAAATATCCG.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCt                                                 <  1:2631767/101‑1 (MQ=255)
                                                      tCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCt                                                 <  2:2302495/101‑1 (MQ=255)
                                                       cTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                                <  1:3529238/101‑1 (MQ=255)
                                                       cTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGCCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                                >  1:2265057/1‑101 (MQ=255)
                                                        ttGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCGGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCTCCttt                                               <  2:1677557/101‑1 (MQ=37)
                                                        ttGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                                               <  1:1265657/101‑1 (MQ=255)
                                                         tggcGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTg                                              >  1:2420108/4‑101 (MQ=255)
                                                         tGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTg                                              >  1:1651336/1‑101 (MQ=255)
                                                         tGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTg                                              >  2:2885210/1‑101 (MQ=255)
                                                         tGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTg                                              >  1:2210826/1‑101 (MQ=255)
                                                         tGCCAAAAATATCCG.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTg                                              <  2:2924812/101‑1 (MQ=38)
                                                          gCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGt                                             >  2:3357811/1‑101 (MQ=255)
                                                           ccGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc                                            >  1:3153813/1‑101 (MQ=255)
                                                              aaaaTATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGGGCCCCTTTGTCAtt                                         >  1:1053140/1‑101 (MQ=255)
                                                                 atatCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCNTNCtn                                      >  1:1058683/1‑100 (MQ=255)
                                                                   ttCCG.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc                                    <  1:3364653/100‑1 (MQ=255)
                                                                      cc.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCa                                 <  1:4053584/101‑1 (MQ=255)
                                                                      cc.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGGGCCCCTTTGTCATTCTGTCGCa                                 >  1:3286465/1‑101 (MQ=255)
                                                                       c.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc                                <  2:2787310/101‑1 (MQ=255)
                                                                          gggggTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                              <  2:1917964/101‑1 (MQ=255)
                                                                          gggggTAAA.GGGCGCA..GCCGGGGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                              <  2:3553598/101‑1 (MQ=255)
                                                                           ggggTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCa                             >  2:3715464/1‑101 (MQ=255)
                                                                           ggggTGAA.TGGCGCA..GCCAGAGGGGGCCACCCC.C.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCa                             <  2:514208/101‑1 (MQ=255)
                                                                            gggTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt                            <  2:982947/101‑1 (MQ=255)
                                                                            gggTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt                            >  1:1931481/1‑101 (MQ=255)
                                                                            gggTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt                            <  2:1706323/101‑1 (MQ=255)
                                                                             ggTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATg                           <  2:602448/101‑1 (MQ=255)
                                                                             ggTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATg                           <  2:3299626/101‑1 (MQ=255)
                                                                              gTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGt                          <  1:3028008/101‑1 (MQ=255)
                                                                               tGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTc                         >  1:1124005/1‑101 (MQ=255)
                                                                               tGAA.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTc                         >  2:1436569/1‑101 (MQ=255)
                                                                                ttccgatctcA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTc                         <  2:4117340/92‑1 (MQ=255)
                                                                                 aa.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt                       >  1:779448/1‑101 (MQ=255)
                                                                                  a.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATa                      >  1:1882425/1‑101 (MQ=255)
                                                                                  a.TGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATa                      <  1:3246538/101‑1 (MQ=255)
                                                                                    tGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa                     <  2:3818028/101‑1 (MQ=255)
                                                                                    tGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa                     <  2:289922/101‑1 (MQ=255)
                                                                                    tGGCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa                     <  1:675004/101‑1 (MQ=255)
                                                                                     ggCGCA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAg                    <  2:1882305/101‑1 (MQ=255)
                                                                                       cgcA..GCCAGAGGGGGGGA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGATCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCt                  >  2:1289080/1‑101 (MQ=255)
                                                                                       cgcA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCt                  <  1:2475575/101‑1 (MQ=255)
                                                                                        gcA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTg                 <  1:1251423/101‑1 (MQ=255)
                                                                                         cA..GCCAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTgg                <  2:2768367/101‑1 (MQ=255)
                                                                                               cAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGc            >  1:2576725/1‑101 (MQ=255)
                                                                                               cAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGc            >  1:3135784/1‑101 (MQ=255)
                                                                                               cAGAGGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGCCATTCTGTCGCACCCATGTCATAAGCTGGCCGc            >  2:2037613/1‑101 (MQ=255)
                                                                                                agagGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCa           >  1:3947271/1‑101 (MQ=255)
                                                                                                  ggggggcCA.CCC.C.C.CCCTTCCGGGGGCGCCCGCGCCGCGCGCCGAAACAGGTCGTCCCCCTTTGTCATTCTGTCGCCCCCATGTCATAAGCTGGCCGCATc         <  2:421156/100‑1 (MQ=255)
                                                                                                  agGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGCCGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATc         >  1:738113/1‑101 (MQ=255)
                                                                                                  agGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATc         <  1:4102452/101‑1 (MQ=255)
                                                                                                  agGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATc         >  1:2171786/1‑101 (MQ=255)
                                                                                                  agGGGGGCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATc         <  1:334245/101‑1 (MQ=255)
                                                                                                     ggggCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGAt      <  1:484674/101‑1 (MQ=255)
                                                                                                     ggggCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGAt      <  1:3752511/101‑1 (MQ=255)
                                                                                                        gCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTc   >  2:2990073/1‑101 (MQ=255)
                                                                                                        gCA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTc   <  2:84115/101‑1 (MQ=255)
                                                                                                         cA.GCGCC.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTcc  <  2:3537153/101‑1 (MQ=255)
                                                                                                               |                                                                                                  
TGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCA.GCG.C.C.CCCTT.CGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTCC  >  NC_014034/2249291‑2249486

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG