Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,252,630 +G 100% coding (223/1050 nt) ltaE → threonine aldolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,252,6301.→G100.0% 459.6 120coding (223/1050 nt)ltaEthreonine aldolase
Reads supporting (aligned to +/- strand):  new base (50/70):  ref base (0/0):  total (50/70)

Only 100 of 143 total aligned reads displayed.

GCATCTGCGCGAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCT.GGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCAAGCTGGTGCTGG  >  NC_014034/2252530‑2252723
                                                                                                     |                                                                                             
gCATCTGCGCGAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCt                                                                                                <  1:1773140/101‑1 (MQ=255)
gCATCTGCGCGAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCt                                                                                                <  1:4051749/101‑1 (MQ=255)
 cATCTGCGCGAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCT.g                                                                                              >  1:2653559/1‑101 (MQ=255)
 cATCTGCGCGAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCT.g                                                                                              >  1:2787482/1‑101 (MQ=255)
  aTCTGCGCGAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCT.gg                                                                                             <  1:1249967/101‑1 (MQ=255)
  aTCTGCGCGAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCT.gg                                                                                             >  2:476619/1‑101 (MQ=255)
    ntGCNCGAGATTTTCGAGGCGCCGCAGGCCGTGATCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCT.gggg                                                                                           <  1:3650872/100‑1 (MQ=255)
    cTGCGCGAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCT.gggg                                                                                           <  1:2963246/101‑1 (MQ=255)
      gcgcGAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCT.gggggg                                                                                         >  1:2712202/1‑101 (MQ=255)
       cgcgAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCT.ggggggg                                                                                        <  1:3847542/101‑2 (MQ=255)
        gcgAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCT.gggggggc                                                                                       <  2:3103702/101‑3 (MQ=255)
        gcgAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCT.gggggggc                                                                                       <  2:3627927/101‑3 (MQ=255)
         cgAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCT.gggggggcg                                                                                      <  2:1026307/101‑4 (MQ=255)
          gagaTTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCT.gggggggcga                                                                                     >  2:4232729/1‑97 (MQ=255)
          gagaTTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCT.gggggggcga                                                                                     >  2:3117570/1‑97 (MQ=255)
             aTTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGAttt                                                                                   >  2:1368997/1‑101 (MQ=255)
              ttttCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGAtttt                                                                                  <  1:2333960/101‑1 (MQ=255)
              ttttCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGAtttt                                                                                  >  2:1464933/1‑101 (MQ=255)
              ttttCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGAtttt                                                                                  >  1:1231819/1‑101 (MQ=255)
              ttttCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGAtttt                                                                                  >  2:938917/1‑101 (MQ=255)
                ttCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCt                                                                                >  1:3882935/1‑101 (MQ=255)
                 tCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTg                                                                               >  2:71139/1‑101 (MQ=255)
                  cGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGGTTTTCTGt                                                                              >  1:1931117/1‑101 (MQ=255)
                    aGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCa                                                                            <  2:397844/101‑1 (MQ=255)
                    aGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCa                                                                            >  1:1586407/1‑101 (MQ=255)
                    aGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCa                                                                            <  1:102233/101‑1 (MQ=255)
                    aGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCa                                                                            <  1:3371355/101‑1 (MQ=255)
                     ggCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCAc                                                                           <  2:343299/101‑1 (MQ=255)
                       cgccgcAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCg                                                                         <  2:2682452/101‑1 (MQ=255)
                         ccgcAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCa                                                                       >  1:1468131/1‑101 (MQ=255)
                           gcAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAAc                                                                     <  1:1524560/101‑1 (MQ=255)
                           gcAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAAc                                                                     <  1:1167953/101‑1 (MQ=255)
                            cAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCGGTCACCGCAACg                                                                    >  2:1849572/1‑101 (MQ=255)
                             aGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGc                                                                   >  1:1388472/1‑101 (MQ=255)
                             aGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGc                                                                   <  2:175158/101‑1 (MQ=255)
                              ggCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGcc                                                                  <  2:2817925/101‑1 (MQ=255)
                               gCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGccc                                                                 <  1:1282560/101‑1 (MQ=255)
                               gCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGacc                                                                 <  2:3921076/101‑1 (MQ=255)
                                gcGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCa                                                                <  2:466994/100‑1 (MQ=255)
                                  gTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCata                                                              <  2:3053677/101‑1 (MQ=255)
                                  gTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCata                                                              <  1:3028361/101‑1 (MQ=255)
                                   tGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCatat                                                             >  1:3059541/1‑101 (MQ=255)
                                   tGGTCTATATGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCatat                                                             >  2:122175/1‑101 (MQ=255)
                                    ggTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATc                                                            >  2:273594/1‑101 (MQ=255)
                                    ggTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATc                                                            <  2:1586298/101‑1 (MQ=255)
                                    ggTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATc                                                            <  2:4202788/101‑1 (MQ=255)
                                    cttCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATc                                                            <  1:4152543/99‑1 (MQ=255)
                                      tCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGa                                                          >  1:442580/1‑101 (MQ=255)
                                        tATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAgg                                                        <  2:3572873/101‑1 (MQ=255)
                                           cTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAg                                                     >  2:2138144/1‑101 (MQ=255)
                                           cTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAg                                                     <  2:1258935/101‑1 (MQ=255)
                                           cTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAg                                                     <  2:1231723/101‑1 (MQ=255)
                                           cTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAg                                                     <  2:4187970/101‑1 (MQ=255)
                                             ggtggCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGAc                                                   >  1:4028724/1‑101 (MQ=255)
                                                ggCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGaa                                                <  2:1410151/101‑1 (MQ=255)
                                                 gCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAAt                                               <  1:1017868/101‑1 (MQ=255)
                                                 gCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAAt                                               <  1:3203923/101‑1 (MQ=255)
                                                  cGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATg                                              <  2:791205/101‑1 (MQ=255)
                                                  cGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATg                                              <  1:362136/101‑1 (MQ=255)
                                                   gACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATgc                                             >  1:2608938/1‑101 (MQ=255)
                                                   gACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATgc                                             <  2:2945354/101‑1 (MQ=255)
                                                    aCGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATgcg                                            >  2:1467343/1‑101 (MQ=255)
                                                      gggCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATgcggc                                          >  1:1312819/1‑101 (MQ=255)
                                                        gCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGgcgc                                        >  1:3241544/1‑101 (MQ=255)
                                                          tccgatctgAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGccc                                      <  1:3053800/93‑1 (MQ=255)
                                                          aCGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGccc                                      <  2:1025694/101‑1 (MQ=255)
                                                          aCGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGccc                                      <  1:2340904/101‑1 (MQ=255)
                                                            ggcggcGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGccccc                                    >  2:2512128/1‑101 (MQ=255)
                                                            ggcggcGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGccccc                                    <  1:939002/101‑1 (MQ=255)
                                                            ggcggcGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGccccc                                    <  1:1707964/101‑1 (MQ=255)
                                                             gcggcgAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCg                                   >  2:901962/1‑101 (MQ=255)
                                                                gcgAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAAt                                <  1:4043445/101‑1 (MQ=255)
                                                                gcgAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAAt                                <  2:1703349/101‑1 (MQ=255)
                                                                 cgAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAAtt                               <  2:4028463/101‑1 (MQ=255)
                                                                  gAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGcccagatcgga                              >  2:3053575/1‑93 (MQ=255)
                                                                  gAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTc                              <  2:2820313/101‑1 (MQ=255)
                                                                  gAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTc                              >  2:2088211/1‑101 (MQ=255)
                                                                     tGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTac                           <  2:3746438/101‑1 (MQ=255)
                                                                      gcgcTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTaca                          <  1:3509561/101‑1 (MQ=255)
                                                                       cgcTCTCCTGTGCGTTGCTTACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTacac                         <  2:2444947/101‑1 (MQ=255)
                                                                         ctctCCTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTTACCGCAAAGCACATGTCGAGGGAGCCGAATGCGGAGGCCCCGAATTCttgacgg                       >  1:1986344/1‑95 (MQ=255)
                                                                             ccTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACcggcgg                   >  1:3375531/1‑101 (MQ=255)
                                                                             ccTGTGCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACcggcgg                   >  1:640233/1‑101 (MQ=255)
                                                                               tgtgCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCt                 >  1:2470600/1‑101 (MQ=255)
                                                                               tgtgCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCt                 <  1:776837/101‑1 (MQ=255)
                                                                               tgtgCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCt                 >  1:2468027/1‑101 (MQ=255)
                                                                                gtgCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTc                <  1:2150326/101‑1 (MQ=255)
                                                                                 tgCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTcc               <  1:2138289/101‑1 (MQ=255)
                                                                                 tgCGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTcc               <  2:401726/101‑1 (MQ=255)
                                                                                   cGGTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCaa             <  1:476676/101‑1 (MQ=255)
                                                                                    ggTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCAAg            >  1:3267239/1‑101 (MQ=255)
                                                                                     gTGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCAAgc           >  1:676011/1‑101 (MQ=255)
                                                                                      tGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCAAgct          <  2:3326360/101‑1 (MQ=255)
                                                                                      tGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCAAgct          >  1:4160375/1‑101 (MQ=255)
                                                                                      tGCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCAAgct          <  1:1012583/101‑1 (MQ=255)
                                                                                       gCTGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCAAgctg         >  2:2388228/1‑101 (MQ=255)
                                                                                         tGACCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCAAgctggt       >  1:675534/1‑101 (MQ=255)
                                                                                           aCCCAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCAAgctggtgc     <  2:1800334/101‑1 (MQ=255)
                                                                                             ccAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCAAgctggtgctg   >  1:2642504/1‑101 (MQ=255)
                                                                                              cAGCCCTGGGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCAAgctggtgctgg  <  1:2088349/101‑1 (MQ=255)
                                                                                                     |                                                                                             
GCATCTGCGCGAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCT.GGGGGGCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCAAGCTGGTGCTGG  >  NC_014034/2252530‑2252723

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG