Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,287,698 +G 37.5% intergenic (‑95/+140) RCAP_rcc02125 ← / ← acsA1 hypothetical protein/acetate‑‑CoA ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,287,6982.→G37.5% 5.9 0.0 ‑0.28intergenic (‑95/+140)RCAP_rcc02125/acsA1hypothetical protein/acetate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (5/0):  total (8/0)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.59e-01

TTCCTCCTCGTTGAAGTCGTCCGCTCCGGCTTGCGCGGCGCGGTTTGGCCGTGTTGCCCCGGCCGTTGCTCTTGACCCGCCCGTAGCGGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTAGTT.GCGCCAGGAAAAAGCACAGA.CTTTTGCCCGTTCACCCTGGCGCAAATCTCCC  >  NC_014034/2287609‑2287786
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ttCCTCCTCGTTGAAGTCGTCCGCTCCGGCTTGCGCGGCGCGGTTTGGCCGTGTTGCCCCGGCCGTTGCTCTTGACCCGCCCGTAGCGGC..cggcgggctgg                                                                                 >  1:1506348/1‑93 (MQ=0)
       tcGTTGAAGTCGTCCGCTCCGGCTTGCGCGGCGCGGTTTTGCCGTGTTGCCCCGGCCGTTGCTCTTGACCCGCCCGTAGCGGT..GGGGGGGTTCcacccccc                                                                          >  2:659757/1‑100 (MQ=1)
         gTTGAAGTCGTCCGCTCCGGCTTGCGCGGCGCGGTTTGGCCGTGTTGCCCCGGCCGTTGCTCTTGACCCGCCCGTAGCGGC..GGGGGGGTTCcacccccccc                                                                        >  1:578350/1‑98 (MQ=1)
                           ggCTTGCGCGGCGCGGTTTGGCCGTGTTGCCCCGGCCGTTGCTCTTGACCCGCCCGTAGCGGCGGGGGGGTTTCA.ACCCCCCCCCCCCCCCGGCgcgtggg                                                       >  1:949384/1‑95 (MQ=1)
                                      cgcggTTTGGCCGTGTTGCCCCGGCCGTTGCTCTTGACCCGCCCGTAGCGGC..GGGGGGGTCTCACCCCCCCACCCCCCCGGCGGGGTG.CGGCCCGGaaaaa                                          >  2:722696/1‑101 (MQ=1)
                                      cgcggTTTGGCCGTGTTGCCCCGGCCGTTGCTCTTGACCCGCCCGTAGCGGC..GGGGGGGTCACACCCCCCCACCCCCCTGGGGTGGTT.GTGGCGGGgaaaa                                          >  2:2645341/1‑101 (MQ=1)
                                           tttGGCCGTGTTGCCCCGGCCGTTGCTCTTGACCCGCCCGTAGCGGCGGGGGGGTTTCACACCCCCCCCCCCCCGGGGCGGGTTG.GCCCCGGGaaaaaacc                                       >  1:3337690/1‑98 (MQ=0)
                                            ttGGCCGTGTTGCCCCGGCCGTTGCTCTTGACCCGCCCGTAGCGGCGGGGGGGTTTCACCCCCCCCCCCCCCCCGGGGGGGGTT.GC.CCGGGaaaaaaaaaa                                     >  2:2180847/1‑96 (MQ=0)
                                                                   gCTCTTGACCCGCCCGTAGCGGCG.GGGGGGTTCCCCCCCCCGC.CCCCCCCGGCGGGGTTGGCCCCAGGAAAAAAAAAGAA.CTTTTTCCCTTTCCccccgcg             >  1:3518148/1‑97 (MQ=0)
                                                                             cgcccgTAGCGGC..GGGGGGGTTTCACCCCCCCCCCCCCCCGGAGGAGGT.GCGCCCGGGAAAAACAAAGACCTTTTCCCCCTTC.CCCCCGCGCACAACTccc  >  2:2155086/1‑101 (MQ=0)
                                                                                          |                                                                                           
TTCCTCCTCGTTGAAGTCGTCCGCTCCGGCTTGCGCGGCGCGGTTTGGCCGTGTTGCCCCGGCCGTTGCTCTTGACCCGCCCGTAGCGGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTAGTT.GCGCCAGGAAAAAGCACAGA.CTTTTGCCCGTTCACCCTGGCGCAAATCTCCC  >  NC_014034/2287609‑2287786

Base quality scores:  ATCG  < 3 ≤  ATCG  < 10 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG