Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_014034 | 2,287,698 | +G | 37.5% | intergenic (‑95/+140) | RCAP_rcc02125 ← / ← acsA1 | hypothetical protein/acetate‑‑CoA ligase |
Read alignment evidence... | ||||||||||
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seq id | position | change | freq | score | reads | annotation | genes | product | ||
* | NC_014034 | 2,287,698 | 2 | .→G | 37.5% | 5.9 0.0 ‑0.2 | 8 | intergenic (‑95/+140) | RCAP_rcc02125/acsA1 | hypothetical protein/acetate‑‑CoA ligase |
Reads supporting (aligned to +/- strand): new base (3/0): ref base (5/0): total (8/0) | ||||||||||
Fisher's exact test strand distribution p-value = 1.00e+00 | ||||||||||
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.59e-01 |
TTCCTCCTCGTTGAAGTCGTCCGCTCCGGCTTGCGCGGCGCGGTTTGGCCGTGTTGCCCCGGCCGTTGCTCTTGACCCGCCCGTAGCGGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTAGTT.GCGCCAGGAAAAAGCACAGA.CTTTTGCCCGTTCACCCTGGCGCAAATCTCCC > NC_014034/2287609‑2287786
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ttCCTCCTCGTTGAAGTCGTCCGCTCCGGCTTGCGCGGCGCGGTTTGGCCGTGTTGCCCCGGCCGTTGCTCTTGACCCGCCCGTAGCGGC..cggcgggctgg > 1:1506348/1‑93 (MQ=0)
tcGTTGAAGTCGTCCGCTCCGGCTTGCGCGGCGCGGTTTTGCCGTGTTGCCCCGGCCGTTGCTCTTGACCCGCCCGTAGCGGT..GGGGGGGTTCcacccccc > 2:659757/1‑100 (MQ=1)
gTTGAAGTCGTCCGCTCCGGCTTGCGCGGCGCGGTTTGGCCGTGTTGCCCCGGCCGTTGCTCTTGACCCGCCCGTAGCGGC..GGGGGGGTTCcacccccccc > 1:578350/1‑98 (MQ=1)
ggCTTGCGCGGCGCGGTTTGGCCGTGTTGCCCCGGCCGTTGCTCTTGACCCGCCCGTAGCGGCGGGGGGGTTTCA.ACCCCCCCCCCCCCCCGGCgcgtggg > 1:949384/1‑95 (MQ=1)
cgcggTTTGGCCGTGTTGCCCCGGCCGTTGCTCTTGACCCGCCCGTAGCGGC..GGGGGGGTCTCACCCCCCCACCCCCCCGGCGGGGTG.CGGCCCGGaaaaa > 2:722696/1‑101 (MQ=1)
cgcggTTTGGCCGTGTTGCCCCGGCCGTTGCTCTTGACCCGCCCGTAGCGGC..GGGGGGGTCACACCCCCCCACCCCCCTGGGGTGGTT.GTGGCGGGgaaaa > 2:2645341/1‑101 (MQ=1)
tttGGCCGTGTTGCCCCGGCCGTTGCTCTTGACCCGCCCGTAGCGGCGGGGGGGTTTCACACCCCCCCCCCCCCGGGGCGGGTTG.GCCCCGGGaaaaaacc > 1:3337690/1‑98 (MQ=0)
ttGGCCGTGTTGCCCCGGCCGTTGCTCTTGACCCGCCCGTAGCGGCGGGGGGGTTTCACCCCCCCCCCCCCCCCGGGGGGGGTT.GC.CCGGGaaaaaaaaaa > 2:2180847/1‑96 (MQ=0)
gCTCTTGACCCGCCCGTAGCGGCG.GGGGGGTTCCCCCCCCCGC.CCCCCCCGGCGGGGTTGGCCCCAGGAAAAAAAAAGAA.CTTTTTCCCTTTCCccccgcg > 1:3518148/1‑97 (MQ=0)
cgcccgTAGCGGC..GGGGGGGTTTCACCCCCCCCCCCCCCCGGAGGAGGT.GCGCCCGGGAAAAACAAAGACCTTTTCCCCCTTC.CCCCCGCGCACAACTccc > 2:2155086/1‑101 (MQ=0)
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TTCCTCCTCGTTGAAGTCGTCCGCTCCGGCTTGCGCGGCGCGGTTTGGCCGTGTTGCCCCGGCCGTTGCTCTTGACCCGCCCGTAGCGGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTAGTT.GCGCCAGGAAAAAGCACAGA.CTTTTGCCCGTTCACCCTGGCGCAAATCTCCC > NC_014034/2287609‑2287786
Base quality scores: 
ATCG
< 3 ≤
ATCG
< 10 ≤
ATCG
< 23 ≤
ATCG
< 33 ≤
ATCG
< 41 ≤
ATCG
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