Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,304,888 +G 100% coding (2687/2808 nt) RCAP_rcc02139 ← methyl‑accepting chemotaxis protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,304,8881.→G100.0% 615.0 172coding (2687/2808 nt)RCAP_rcc02139methyl‑accepting chemotaxis protein
Reads supporting (aligned to +/- strand):  new base (86/86):  ref base (0/0):  total (86/86)

Only 100 of 204 total aligned reads displayed.

GGCGGACAAGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATGC.GAAG.ACCG.CGTACGCTCCCGCA.G.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGG  >  NC_014034/2304787‑2304981
                                                                                                      |                                                                                                 
gggagACAAGGGAAAAATCGTGATAATCTTGCGAAATACTAGGCGGGATCCGTCTTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGc                                                                                                    <  1:3025630/97‑1 (MQ=255)
 gcggACAAGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGC.g                                                                                                  <  1:2135563/101‑1 (MQ=255)
  cggACAAGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGGGGCG.CCTGCCTGC.gg                                                                                                 >  1:4197654/1‑100 (MQ=255)
   ggACAAGGGCAAGATCCTGATAATCTTGCGCAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGC.gga                                                                                                <  1:407755/101‑3 (MQ=255)
   ggACAAGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGC.gga                                                                                                >  2:4012321/1‑99 (MQ=255)
   ggACAAGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGC.gga                                                                                                >  1:2722696/1‑99 (MQ=255)
   ggACAAGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGGGGCGCCC.GCATGC.gga                                                                                                >  1:3717152/1‑99 (MQ=255)
   ggACAAGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGGCAGCCGGCCTGCCGCAACGGCGGCGCCGGGGGCG.CCTGCCTGC.gga                                                                                                >  2:193312/1‑99 (MQ=255)
   gaccAAGGGCATAATCATGAGAATCTGGCGCAAACCTGGGCGGGACCCGGCGTGTCCGCGGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGC.gga                                                                                                <  2:2607163/98‑3 (MQ=255)
    gACAAGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGGGGCG.CCTGCATGC.ggaa                                                                                               >  2:922438/1‑98 (MQ=255)
     aCAAGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGC.ggaag                                                                                              <  1:1128931/101‑5 (MQ=255)
      cAAGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGGCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGCGGCGCCC.GCATGC.ggaaga                                                                                             >  1:2084992/1‑96 (MQ=255)
      cAAGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGC.ggaaga                                                                                             >  1:1573506/1‑96 (MQ=255)
       aaGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.Ac                                                                                            >  1:2177070/1‑101 (MQ=255)
        aGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.Acc                                                                                           >  2:3561094/1‑101 (MQ=255)
        aGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.Acc                                                                                           >  1:3365710/1‑101 (MQ=255)
          ggCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACcg.c                                                                                        <  2:2320930/101‑1 (MQ=255)
          ggCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACcg.c                                                                                        >  2:2260886/1‑101 (MQ=255)
           gCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACcg.cc                                                                                       <  1:1894968/101‑2 (MQ=255)
           gCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCGGCATGCGGAAG.ACcg.cc                                                                                       >  1:559141/1‑100 (MQ=255)
             aaGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACcg.ccgt                                                                                     >  2:564459/1‑98 (MQ=255)
             aaGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGGGGCG.CCTGCATGC.GGAG.aacccccgc                                                                                     >  1:3248383/1‑93 (MQ=255)
               gATCCTGATAATCTTGCGCAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACcg.ccgtca                                                                                   <  1:4012581/101‑6 (MQ=255)
                aTCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCCTGCGGAAG.ACcg.ccgtcag                                                                                  >  2:1940275/1‑95 (MQ=255)
                aTCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACcg.ccgtcag                                                                                  >  2:2797321/1‑95 (MQ=255)
                 tCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACcg.ccgtcagc                                                                                 >  1:3369033/1‑94 (MQ=255)
                  ccTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACcg.ccgtcagct                                                                                >  1:3995037/1‑93 (MQ=255)
                   cTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGGGCCGGTGGCG.CCTGCATGCGGAAG.ACcg.ccgtcagctc                                                                               >  2:607943/1‑92 (MQ=255)
                       tAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGc                                                                            >  2:1654165/1‑101 (MQ=255)
                        aaTCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGcc                                                                           >  1:3984558/1‑100 (MQ=255)
                            ttGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCA.g.ggg                                                                     >  2:3149537/1‑99 (MQ=255)
                            ttGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGAGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCA.g.ggt                                                                     >  1:3416738/1‑99 (MQ=255)
                            tggcgcAAAACGGGCCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCA.g.ggt                                                                     <  2:1858023/99‑3 (MQ=255)
                             ggcgcAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCA.g.ggtc                                                                    <  2:3943875/100‑4 (MQ=255)
                               cgcAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCA.g.ggtctg                                                                  <  2:737901/101‑6 (MQ=255)
                                gcAAATCTCGGCCGTCCCCGGCCTGTAAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCTCGCAGG.GTCTgc                                                                  <  1:2286781/101‑1 (MQ=255)
                                gcAAACCCCGGCCGGATCCGGCCTGTCCGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTgc                                                                  <  1:1815334/101‑1 (MQ=255)
                                gaaaacCTCGCCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTgc                                                                  <  2:135859/99‑1 (MQ=255)
                                    accTCGGCCGGATCCGCCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCt                                                              <  2:11309/99‑1 (MQ=255)
                                        cgcccGGATCGGGCCTGTCGGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGcc                                                          <  1:2249712/98‑1 (MQ=255)
                                        cGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGcc                                                          >  2:3353471/1‑101 (MQ=255)
                                         ggCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGccc                                                         >  1:2618640/1‑101 (MQ=255)
                                          gCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCca                                                        <  1:282529/101‑1 (MQ=255)
                                           ccGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCcag                                                       <  1:2715944/101‑1 (MQ=255)
                                            cGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCcagc                                                      >  2:627721/1‑101 (MQ=255)
                                             ggATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GGCTGCGCCTCGCCcagca                                                     >  2:1425162/1‑101 (MQ=255)
                                             ggACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCcagca                                                     <  2:1708936/101‑1 (MQ=255)
                                               aTCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCagcagc                                                   <  1:1310324/101‑1 (MQ=255)
                                                tCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAgcg                                                  <  2:3716898/101‑1 (MQ=255)
                                                tCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGCGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAgcg                                                  >  1:1681307/1‑101 (MQ=255)
                                                 ccGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAgcgc                                                 <  1:3124404/101‑1 (MQ=255)
                                                 ccGGCCTGCCGGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAgcgc                                                 <  2:1681192/101‑1 (MQ=255)
                                                  cGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGcc                                                >  1:1701061/1‑101 (MQ=255)
                                                  cGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGcc                                                >  1:197729/1‑101 (MQ=255)
                                                  cGGCCTGTCAGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGcc                                                <  2:4151736/101‑1 (MQ=255)
                                                   gcccggTCAGCGGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCt                                               <  2:1241249/96‑1 (MQ=255)
                                                    gCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTg                                              >  1:3135457/1‑101 (MQ=255)
                                                       tGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGt                                           <  2:3755411/101‑1 (MQ=255)
                                                        gTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTc                                          >  2:525847/1‑101 (MQ=255)
                                                         tCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTcg                                         >  2:2359472/1‑101 (MQ=255)
                                                         tCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTcg                                         <  2:1921177/101‑1 (MQ=255)
                                                          cacccGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGTGCCTCGCCCAGCAGCGCCCGCGTcgc                                        >  2:1753692/4‑101 (MQ=255)
                                                             ccGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGc                                     <  1:3444036/101‑1 (MQ=255)
                                                               ggCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcg                                   >  2:1282926/1‑101 (MQ=255)
                                                               ggCCGGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcg                                   <  1:3509846/101‑1 (MQ=255)
                                                                gCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcgc                                  <  1:964098/101‑1 (MQ=255)
                                                                gCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcgc                                  >  1:780438/1‑101 (MQ=255)
                                                                  cTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGt                                >  1:2587027/1‑101 (MQ=255)
                                                                     ccGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGtttt                             <  1:3472088/101‑1 (MQ=255)
                                                                     ccGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCCCGGCCGCGtttt                             <  1:2295905/101‑1 (MQ=255)
                                                                      cGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTc                            <  1:3149776/101‑1 (MQ=255)
                                                                      cGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTc                            <  1:1234868/101‑1 (MQ=255)
                                                                      cGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTc                            >  1:3586517/1‑101 (MQ=255)
                                                                      cGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGCTTTc                            >  2:4045153/1‑101 (MQ=255)
                                                                         aaCGGCGGCGCCGGGGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                         >  2:2013540/1‑101 (MQ=255)
                                                                          aCGGCGGCGCCGGTGGAG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCg                        <  1:712084/101‑1 (MQ=255)
                                                                            ggcggcGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGACCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGaa                      >  1:2948039/1‑101 (MQ=255)
                                                                               ggcgCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                   >  2:2629549/1‑101 (MQ=255)
                                                                               ggcgCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                   <  1:44556/101‑1 (MQ=255)
                                                                               gccgcCGGTGGCG.CCTGGATGC.GGAGAACCGCCGTCAACTCCGCCA.GTGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                   <  2:2084854/99‑1 (MQ=255)
                                                                                gcgcCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATc                  <  2:2178054/101‑1 (MQ=25)
                                                                                 cgcCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcg                 >  2:3045002/1‑101 (MQ=255)
                                                                                 cgcCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcg                 <  1:2456202/101‑1 (MQ=25)
                                                                                 cgcCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcg                 >  2:1593255/1‑101 (MQ=255)
                                                                                     ggTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc             <  1:2750481/101‑1 (MQ=25)
                                                                                     ggTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc             >  1:850/1‑101 (MQ=25)
                                                                                     ggTGGCG.CCTGCATGCGGAAG.ACCGCCGCCGGATCCCGCAGG.GGCTGCGCCTCGCCCAGAAGCGCCGGCGTCGCGCCCGCGTTTTCCTCGAACACcggcgc             >  2:2763359/1‑101 (MQ=255)
                                                                                      gTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCa            >  2:1041442/1‑101 (MQ=25)
                                                                                       tGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGACCATTGCCGCAt           >  1:1499310/1‑101 (MQ=255)
                                                                                          gcgccTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtg         >  1:3242709/4‑101 (MQ=25)
                                                                                           cgccTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgt        >  1:411985/3‑101 (MQ=21)
                                                                                           cgccTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgt        <  2:4069494/99‑1 (MQ=25)
                                                                                            gccTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgtg       <  2:3416493/100‑1 (MQ=25)
                                                                                            gccTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgtg       >  1:2210121/2‑101 (MQ=21)
                                                                                             ccTGCATGCGGAAG.CCCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGc      <  2:1890693/101‑1 (MQ=21)
                                                                                              cTGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCt     >  1:1769259/1‑101 (MQ=21)
                                                                                               tGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTg    <  2:3002786/101‑1 (MQ=25)
                                                                                               tGCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTg    <  2:271577/101‑1 (MQ=25)
                                                                                                gCATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTgg   <  1:1593364/101‑1 (MQ=25)
                                                                                                 cATGCGGAAG.ACCGCCGTCAGCTCCCGCAGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTggg  >  2:83888/1‑101 (MQ=21)
                                                                                                      |                                                                                                 
GGCGGACAAGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATGC.GAAG.ACCG.CGTACGCTCCCGCA.G.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGG  >  NC_014034/2304787‑2304981

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG