Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,304,910 +G 100% coding (2665/2808 nt) RCAP_rcc02139 ← methyl‑accepting chemotaxis protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,304,9101.→G100.0% 593.2 170coding (2665/2808 nt)RCAP_rcc02139methyl‑accepting chemotaxis protein
Reads supporting (aligned to +/- strand):  new base (68/100):  ref base (1/1):  total (69/101)

Only 100 of 193 total aligned reads displayed.

AATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATGC.GAAG.ACCG.CGTACGCTCCCG.C.A.G.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCACC  >  NC_014034/2304811‑2305004
                                                                                                         |                                                                                               
aaTCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.a                                                                                                  >  2:2792827/1‑101 (MQ=255)
 aTCTTGCGAAAAACCCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.A.g                                                                                                <  1:438263/101‑1 (MQ=255)
     tGCGCAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.A.g.ggtc                                                                                           <  2:3838919/101‑4 (MQ=255)
      gcgcAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.A.g.ggtct                                                                                          >  1:3392012/1‑97 (MQ=255)
      gcgcAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.A.g.gggct                                                                                          >  1:1890813/1‑97 (MQ=255)
        gcAAACCCCGGCCGGATCCGGCCTGTCCGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTgc                                                                                         <  1:1815334/101‑1 (MQ=255)
        gcAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTgc                                                                                         >  2:233861/1‑101 (MQ=255)
         cAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGACG.ACCGCCGTCAGCTCCCG.C.C.g.gggctgcc                                                                                       >  1:34419/1‑94 (MQ=255)
          aaaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTgcgc                                                                                       <  1:1247941/101‑1 (MQ=255)
            accTCGGCCGGATCCGCCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCt                                                                                     <  2:11309/99‑1 (MQ=255)
              cTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTcc                                                                                   >  1:2842068/1‑100 (MQ=255)
                cGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGcc                                                                                 >  2:3353471/1‑101 (MQ=255)
                 ggCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGccc                                                                                >  1:2618640/1‑101 (MQ=255)
                 ggCCGGATCCGGCCGGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGccc                                                                                <  2:442004/101‑1 (MQ=255)
                  gCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCca                                                                               <  1:2591276/101‑1 (MQ=255)
                  gCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCca                                                                               <  1:282529/101‑1 (MQ=255)
                   ccGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCcag                                                                              <  1:2715944/101‑1 (MQ=255)
                     ggATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCcagca                                                                            >  2:3471836/1‑101 (MQ=255)
                     ggATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCcagca                                                                            >  1:895121/1‑101 (MQ=255)
                     ggACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCcagca                                                                            <  2:1708936/101‑1 (MQ=255)
                      gATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCGGCTCCCG.C.AGG.GCCTGCGCCTCGCCctgcag                                                                           >  2:561861/1‑101 (MQ=255)
                       aTCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCagcagc                                                                          <  1:1310324/101‑1 (MQ=255)
                        tCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGCGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAgcg                                                                         >  1:1681307/1‑101 (MQ=255)
                         ccGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAgcgc                                                                        <  1:3124404/101‑1 (MQ=255)
                          cGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGcc                                                                       >  1:1591463/1‑101 (MQ=255)
                          cGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGcc                                                                       >  1:1701061/1‑101 (MQ=255)
                           ggCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCt                                                                      >  1:2260104/1‑101 (MQ=255)
                           gcccggTCAGCGGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCt                                                                      <  2:1241249/96‑1 (MQ=255)
                            gCCTGTCAGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTg                                                                     <  1:84469/101‑1 (MQ=255)
                              cgggcAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCg                                                                   <  1:3449385/97‑1 (MQ=255)
                                 tCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTcg                                                                <  2:1921177/101‑1 (MQ=255)
                                 tCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTcg                                                                >  2:2359472/1‑101 (MQ=255)
                                 tCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTcg                                                                >  2:737510/1‑101 (MQ=255)
                                 tCACCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTcg                                                                <  2:967615/101‑1 (MQ=255)
                                  cacccGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGTGCCTCGCCCAGCAGCGCCCGCGTcgc                                                               >  2:1753692/4‑101 (MQ=255)
                                   aGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgcg                                                              <  1:2054030/101‑1 (MQ=255)
                                   aGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgcg                                                              >  1:373031/1‑101 (MQ=255)
                                     ccGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGc                                                            <  2:3012732/101‑1 (MQ=255)
                                      cGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGcc                                                           <  1:2793046/101‑1 (MQ=255)
                                       ggCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcg                                                          >  2:2265053/1‑101 (MQ=255)
                                         ccTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcgcg                                                        >  2:1329757/1‑101 (MQ=255)
                                           tGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGtt                                                      >  1:4068347/1‑101 (MQ=255)
                                             ccGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCCCGGCCGCGtttt                                                    <  1:2289488/101‑1 (MQ=255)
                                              cGCACCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTc                                                   <  1:4000245/101‑1 (MQ=255)
                                              cGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTc                                                   >  1:1435506/1‑101 (MQ=255)
                                              cGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTc                                                   <  1:3149776/101‑1 (MQ=255)
                                                 aaCGGCGGCGCCGGTGGCGCCC.GCATGCGGAAG.ACCGCCGTCAGCTCCCC.C.AGG.GTCTGCGCCCCGCCCACCCGCGCCCGCGTCGCGGACGCGTTTTCCTc                                                >  1:1665048/1‑101 (MQ=255)
                                                 aaCGGCGGCGCCGGGGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                                >  2:2013540/1‑101 (MQ=255)
                                                  aCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCg                                               <  1:2750341/101‑1 (MQ=255)
                                                  aCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCg                                               <  2:3744698/101‑1 (MQ=255)
                                                  aCGGCGGCGCCGGTGGAG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCg                                               <  1:712084/101‑1 (MQ=255)
                                                    ggcggcGCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGACCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGaa                                             >  1:2948039/1‑101 (MQ=255)
                                                    ggcggcGCCGGGGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGaa                                             <  2:571331/101‑1 (MQ=255)
                                                      cggcgCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACa                                           <  1:3587679/101‑1 (MQ=255)
                                                       ggcgCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                                          <  1:537416/101‑1 (MQ=255)
                                                       ggcgCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                                          >  2:2629549/1‑101 (MQ=255)
                                                       ggcgCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                                          <  1:2362362/101‑1 (MQ=255)
                                                       ggcgCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                                          <  2:1362240/101‑1 (MQ=255)
                                                       ggcgCCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTAAGCTCCCG.CTG.G.GGCTGCGCCTCGTCCAGCAGCGGCAGCTTCGGGGCCGCGTTTTCCTCAAACAt                                          >  2:1667336/1‑101 (MQ=255)
                                                       gccgcCGGTGGCG.CCTGGATGC.GGAGAACCGCCGTCAACTCCGC.C.A.GTGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                                          <  2:2084854/99‑1 (MQ=255)
                                                        gcgcCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATc                                         <  2:86718/101‑1 (MQ=25)
                                                          gcCGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgc                                       >  2:1025015/1‑101 (MQ=25)
                                                           ccGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgcc                                      >  2:2214179/1‑101 (MQ=25)
                                                            cGGTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                                     <  1:3353716/101‑1 (MQ=25)
                                                             ggTGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc                                    <  2:1934148/101‑1 (MQ=25)
                                                             ggTGGCG.CCTGCATGCGGAAG.ACCGCCGCCGGATCCCG.C.AGG.GGCTGCGCCTCGCCCAGAAGCGCCGGCGTCGCGCCCGCGTTTTCCTCGAACACcggcgc                                    >  2:2763359/1‑101 (MQ=255)
                                                               tGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGACCATTGCCGCAt                                  >  1:1499310/1‑101 (MQ=255)
                                                               tGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAt                                  >  2:4068975/1‑101 (MQ=25)
                                                               tGGCG.CCTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAt                                  >  2:240655/1‑101 (MQ=25)
                                                                  gcgccTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtg                                >  1:1202668/4‑101 (MQ=21)
                                                                  gcgccTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtg                                >  1:3242709/4‑101 (MQ=25)
                                                                   cgccTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgt                               <  1:3500781/99‑1 (MQ=25)
                                                                   cgccTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgt                               >  1:411985/3‑101 (MQ=21)
                                                                    gccTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgtg                              <  1:2130628/100‑1 (MQ=25)
                                                                    gccTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgtg                              <  2:3416493/100‑1 (MQ=25)
                                                                     ccTGCATGCGGAAG.CCCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGc                             <  2:1890693/101‑1 (MQ=21)
                                                                      cTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCt                            >  1:1769259/1‑101 (MQ=21)
                                                                      cTGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCt                            >  2:3045022/1‑101 (MQ=21)
                                                                       tGCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTg                           <  2:271577/101‑1 (MQ=25)
                                                                        gCATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTgg                          >  1:221723/1‑101 (MQ=21)
                                                                         cATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTggg                         >  2:83888/1‑101 (MQ=21)
                                                                         cATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTggg                         <  2:4093316/101‑1 (MQ=25)
                                                                         cATGCGGAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTggg                         <  2:3994777/101‑1 (MQ=25)
                                                                          atgcggAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGt                        <  2:313351/96‑1 (MQ=21)
                                                                           tgcggAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTg                       >  2:1823243/5‑101 (MQ=21)
                                                                             cggAAG.ACCGCCGTCAGCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGAc                     <  1:3521958/99‑1 (MQ=21)
                                                                                     ccgccgtcagCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCa              <  1:3561347/92‑1 (MQ=21)
                                                                                       gccgtcagCTCCCGCC.G.G.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCAAACATCGCCGCATTGTGCTGGGTGACCTGATCAAg            <  2:2841849/94‑1 (MQ=21)
                                                                                         cgtcagCTCCCG.C.AGG.GTCTGCGCCTCGCCTAGCAGCGCCTGAGTCGCGGCCGCGTTTTCCTCGAACATCTCCGCATTGTGCTGGGTGACCTGATCAAGcg          <  2:1686498/96‑2 (MQ=21)
                                                                                         cgtcagCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCt          <  1:1096585/96‑1 (MQ=255)
                                                                                          gtcagCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTg         <  2:1591354/97‑1 (MQ=255)
                                                                                           tcagCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTgg        >  2:3166609/4‑101 (MQ=255)
                                                                                           tcagCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTgg        >  1:214619/4‑101 (MQ=255)
                                                                                           tcagCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTgg        <  2:1498324/98‑1 (MQ=255)
                                                                                            cagCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGt       >  1:2320872/3‑101 (MQ=255)
                                                                                             agCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGtt      <  1:1940401/100‑1 (MQ=255)
                                                                                              gCTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTc     <  1:3045227/101‑1 (MQ=255)
                                                                                               cTCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCa    >  2:1515101/1‑101 (MQ=255)
                                                                                                tCCCG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAc   <  1:2488161/101‑1 (MQ=255)
                                                                                                 cccG.C.AGG.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAcc  <  2:4197392/101‑1 (MQ=255)
                                                                                                         |                                                                                               
AATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATGC.GAAG.ACCG.CGTACGCTCCCG.C.A.G.GTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCACC  >  NC_014034/2304811‑2305004

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG