Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,307,858 +C 2.1% coding (939/1203 nt) RCAP_rcc02140 ← extracellular ligand‑binding receptor

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,307,8581.→C 2.1% 2.2 ‑1.3 ‑1.9143coding (939/1203 nt)RCAP_rcc02140extracellular ligand‑binding receptor
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (82/58):  total (85/58)
Fisher's exact test strand distribution p-value = 2.72e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.24e-02

Only 100 of 178 total aligned reads displayed.

GCAGCGCCGTCAGATAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCGCCG.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGCGAA  >  NC_014034/2307758‑2307954
                                                                                                     |                                                                                                 
gcaggcccGTCAGATAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAg                                                                                                    <  1:3962234/95‑1 (MQ=255)
gCAGCGCCGTCAGATAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGGCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAg                                                                                                    >  1:2945798/1‑101 (MQ=255)
 cAGCGCCGTCAGATAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAg.c                                                                                                  >  1:292022/1‑101 (MQ=255)
  aGCGCCGTCAGATAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAg.cc                                                                                                 >  2:2889432/1‑101 (MQ=255)
  aGCGCCGTCAGATAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAg.cc                                                                                                 <  1:102049/101‑1 (MQ=255)
   gcgcCGTCAGATAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAg.ccc                                                                                                <  1:2129149/101‑2 (MQ=255)
    cgcCGTCAGATAGCGGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAg.cccg                                                                                               <  2:1715709/101‑3 (MQ=255)
        gTCAGATAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAg.cccggctt                                                                                           >  1:695203/1‑95 (MQ=255)
         tCAGATAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAg.cccggcttc                                                                                          >  1:1329053/1‑94 (MQ=255)
         tCAGATAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAg.cccggcttc                                                                                          <  2:2153090/101‑8 (MQ=255)
          cAGATAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCGGATAGCCCTCGAAGGAGACGAAg.cccggcttcg                                                                                         >  2:108352/1‑93 (MQ=255)
          cAGATAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAg.cccggcttcg                                                                                         <  2:4113889/101‑9 (MQ=255)
          cAGATAGCTGGAACGGGCCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGACg.cccggcttcg                                                                                         >  2:307041/1‑93 (MQ=255)
           aGATAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAg.cccggcttcgc                                                                                        >  1:3198683/1‑92 (MQ=255)
           aGATAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAg.cccggcttcgc                                                                                        <  2:1328953/101‑10 (MQ=255)
            gATAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAg.cccggcttcgcc                                                                                       <  1:1502406/101‑11 (MQ=255)
              tAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTcgccgc                                                                                    >  2:3987136/1‑101 (MQ=255)
               aGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCgccgcc                                                                                   <  2:1924322/101‑1 (MQ=255)
               aGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCgccgcc                                                                                   >  2:752315/1‑101 (MQ=255)
                gCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGccgccg                                                                                  >  1:205296/1‑101 (MQ=255)
                gCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGccgccg                                                                                  <  1:4210055/101‑1 (MQ=255)
                 cTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCgg                                                                                 >  2:3362445/1‑101 (MQ=255)
                  tGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGa                                                                                >  2:1349710/1‑101 (MQ=255)
                     aaCGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATcc                                                                             >  2:4163625/1‑101 (MQ=255)
                     aaCGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATcc                                                                             >  2:199081/1‑101 (MQ=255)
                     aaCGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATcc                                                                             >  2:3859222/1‑101 (MQ=255)
                      aCGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCa                                                                            >  2:241369/1‑101 (MQ=255)
                      aCGGGCCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCa                                                                            <  2:1766596/101‑1 (MQ=255)
                       cgggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAg                                                                           <  2:4139148/101‑1 (MQ=255)
                        gggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAgc                                                                          >  2:3643517/1‑101 (MQ=255)
                         ggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAgcg                                                                         >  2:159939/1‑101 (MQ=255)
                         ggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAgcg                                                                         >  1:362881/1‑101 (MQ=255)
                                ttCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAg                                                                  >  1:273719/1‑101 (MQ=255)
                                  cGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAgcg                                                                >  2:807047/1‑101 (MQ=255)
                                   ggCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCTGC.TTCGCCGCCGGATCCAGCGCCGCGAgcgc                                                               <  2:1455896/101‑1 (MQ=255)
                                     ccccGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCCAGcgacg                                                             >  1:954850/1‑101 (MQ=255)
                                      cccGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGcgccgc                                                            >  1:2308011/1‑101 (MQ=255)
                                      cccGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGcgccgc                                                            >  2:269380/1‑101 (MQ=255)
                                        cgccgcGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCGGATAGCCCTCGAAGGAGACCAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCt                                                          <  1:3613624/101‑1 (MQ=255)
                                        cgccgcGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCt                                                          >  1:4049705/1‑101 (MQ=255)
                                           cgcgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGAt                                                       >  2:3589721/1‑101 (MQ=255)
                                           cgcgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGAt                                                       <  2:3567290/101‑1 (MQ=255)
                                            gcgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATa                                                      >  2:745510/1‑101 (MQ=255)
                                             cgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAg                                                     <  1:3067016/101‑1 (MQ=255)
                                             cgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAg                                                     <  1:3016907/101‑1 (MQ=255)
                                              ggCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgc                                                    >  1:188371/1‑101 (MQ=255)
                                               gccagcgccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgcg                                                   <  1:226863/101‑1 (MQ=255)
                                               gccagcgccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgcg                                                   >  1:1319361/1‑101 (MQ=255)
                                                 cagcgccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgcgcg                                                 <  1:241414/101‑1 (MQ=255)
                                                    cgccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGcca                                              <  2:2532583/101‑1 (MQ=255)
                                                    cgccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGcca                                              >  1:2905575/1‑101 (MQ=255)
                                                     gccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGccac                                             <  1:3836784/101‑1 (MQ=255)
                                                         ccagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCaccacc                                         >  2:3633570/1‑101 (MQ=255)
                                                          cagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCg                                        >  2:2474268/1‑101 (MQ=255)
                                                          cagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCg                                        <  1:3819617/101‑1 (MQ=255)
                                                           agcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCgg                                       >  2:874502/1‑101 (MQ=255)
                                                             cgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGaa                                     >  2:853202/1‑101 (MQ=255)
                                                              gccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAAt                                    >  1:3705320/1‑101 (MQ=255)
                                                              gccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAAt                                    <  1:1158081/101‑1 (MQ=255)
                                                                cagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGt                                  >  2:1712707/1‑101 (MQ=255)
                                                                 agACGGCCGGTCAGATAGCCCTCGAAGGAGACGGAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTc                                 <  1:3643770/101‑1 (MQ=255)
                                                                 agACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTc                                 >  1:175042/1‑101 (MQ=255)
                                                                  gACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCg                                <  2:3177/101‑1 (MQ=255)
                                                                    cGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGgt                              >  1:630382/1‑101 (MQ=255)
                                                                      gCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC..TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGgtgt                            >  1:781193/1‑101 (MQ=255)
                                                                       ccGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTc                           <  1:853283/101‑1 (MQ=255)
                                                                        cGGTCAGATAGCCCTCGAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGNTAGCGCGCCACCACCGGAATGTCGGTGTCg                          >  1:194490/1‑101 (MQ=255)
                                                                         ggTCAGATAGCCCTCGAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGa                         >  1:539699/1‑101 (MQ=255)
                                                                          gTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTGGGTGTCGaa                        >  1:4036377/1‑101 (MQ=255)
                                                                          gTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGaa                        <  1:3589974/101‑1 (MQ=255)
                                                                           tCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAAc                       <  1:2350179/101‑1 (MQ=255)
                                                                           tCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAAc                       <  2:2438800/101‑1 (MQ=255)
                                                                            cAGATAGCCCTCGAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACg                      >  1:3429279/1‑101 (MQ=255)
                                                                            cAGATAGCCCTCGAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACg                      >  1:1253578/1‑101 (MQ=255)
                                                                             aGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACgg                     >  1:1424183/1‑101 (MQ=255)
                                                                              gATAGCCCTCGAAGGAGACGAAGCCCGGC..TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGACTGTCGGTGTCGAACGGa                    >  2:3807772/1‑101 (MQ=255)
                                                                               aTAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGAt                   <  2:1227527/101‑1 (MQ=255)
                                                                                tAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATa                  <  1:2045203/101‑1 (MQ=255)
                                                                                tAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATa                  <  2:781115/101‑1 (MQ=255)
                                                                                 aGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAg                 >  1:1351995/1‑101 (MQ=255)
                                                                                 aGCCCTCGAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAg                 >  1:3249874/1‑101 (MQ=255)
                                                                                    ccTCGAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGc              >  2:2212786/1‑101 (MQ=255)
                                                                                      tCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCAc            >  2:1303970/1‑101 (MQ=255)
                                                                                      tCGAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCAc            >  2:1432891/1‑101 (MQ=255)
                                                                                       cGAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACg           >  2:3439344/1‑101 (MQ=255)
                                                                                        gAAGGAGACGAAGCCCGGC..TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGa          >  2:2982759/1‑101 (MQ=255)
                                                                                        gAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGGCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGa          >  1:2807891/1‑101 (MQ=255)
                                                                                        gAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCGCGa          >  2:3605262/1‑101 (MQ=255)
                                                                                        gAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGa          >  1:1632646/1‑101 (MQ=255)
                                                                                        gAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGa          >  2:3680354/1‑101 (MQ=255)
                                                                                        gAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGa          >  1:1110896/1‑101 (MQ=255)
                                                                                         aaGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGAc         >  1:3713423/1‑101 (MQ=255)
                                                                                          aGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCAACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGAcc        >  1:3864106/1‑101 (MQ=255)
                                                                                           ggAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCt       <  2:1813478/101‑1 (MQ=255)
                                                                                            gagaCGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTg      >  2:3289550/1‑101 (MQ=255)
                                                                                             ggaCGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGc     <  2:3326436/100‑1 (MQ=255)
                                                                                             agaCGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGc     <  1:4163887/101‑1 (MQ=255)
                                                                                             agaCGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGACAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGGTAGGGCACGACCGGc     >  2:3784458/1‑101 (MQ=255)
                                                                                              gaCGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGCg    >  1:65863/1‑101 (MQ=255)
                                                                                                cGAAG.CCGGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGCGaa  <  2:767950/101‑1 (MQ=255)
                                                                                                     |                                                                                                 
GCAGCGCCGTCAGATAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCGCCG.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGCGAA  >  NC_014034/2307758‑2307954

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG