Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,420,305 +G 1.8% intergenic (‑99/+93) RCAP_rcc02245 ← / ← cysE2 major membrane protein I/serine O‑acetyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,420,3050.→G 1.8% 2.2 0.0 ‑0.1167intergenic (‑99/+93)RCAP_rcc02245/cysE2major membrane protein I/serine O‑acetyltransferase
Reads supporting (aligned to +/- strand):  new base (1/2):  ref base (64/100):  total (65/102)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.33e-01

Only 100 of 171 total aligned reads displayed.

AGCCAGCGCGGCAGTGATCGTGGGCCAGGATACGGCGCCCGTCTTCGTGGCATTGGCAAAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAGTTTGACACCGGTTCGGTCATTCACGTTCTCCTTCTTGGGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGTTCTG  >  NC_014034/2420208‑2420395
                                                                                                                                                                                            
agccagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGt                                                                                  >  1:357224/1‑101 (MQ=255)
agccagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGt                                                                                  <  1:2389475/101‑1 (MQ=255)
agccagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGt                                                                                  <  2:2293406/101‑1 (MQ=255)
 gccagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTc                                                                                 <  1:2319560/101‑1 (MQ=255)
 gccagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTc                                                                                 >  1:1570967/1‑101 (MQ=255)
  ccagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCa                                                                                <  1:3220932/101‑1 (MQ=255)
  ccagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCa                                                                                <  1:2802461/101‑1 (MQ=255)
  ccagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCa                                                                                <  1:2491225/101‑1 (MQ=255)
    agcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCAtt                                                                              <  2:2218145/101‑1 (MQ=255)
    agcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCAtt                                                                              <  1:1821373/101‑1 (MQ=255)
     gcgcGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTTGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTc                                                                             <  2:87309/101‑1 (MQ=255)
      cgcgGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCCTTCa                                                                            >  1:3543862/1‑101 (MQ=255)
        gcggcgTGATCGTGTG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCAc                                                                           <  2:2089499/96‑1 (MQ=255)
        gcggcgTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCAc                                                                           <  1:1933878/96‑1 (MQ=255)
          ggcgTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCCG.TTGAC.GCGGTTCGGTCCTTCACGt                                                                         >  1:802435/4‑101 (MQ=255)
          ggcgTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGt                                                                         <  1:1397545/98‑1 (MQ=255)
          ggcgTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGt                                                                         <  2:2030357/98‑1 (MQ=255)
            ggTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTtc                                                                       <  1:3848156/100‑1 (MQ=255)
            cgTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTtc                                                                       >  2:2641121/2‑101 (MQ=255)
            cgTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTtc                                                                       <  2:1423616/100‑1 (MQ=255)
             gTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTtct                                                                      >  1:2232687/1‑101 (MQ=255)
              tGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTtctc                                                                     >  1:2516760/1‑101 (MQ=255)
              atctCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTtctc                                                                     <  2:1108867/98‑1 (MQ=255)
               gATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTcc                                                                    >  1:271863/1‑101 (MQ=255)
                aTCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCct                                                                   <  2:3667031/101‑1 (MQ=255)
                 tCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCctt                                                                  <  1:3000295/101‑1 (MQ=255)
                  cGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTtctcagat                                                                 >  1:1108961/1‑97 (MQ=255)
                  cGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCcttc                                                                 <  2:525656/101‑1 (MQ=255)
                  cGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCcttc                                                                 >  2:4110986/1‑101 (MQ=255)
                   gTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCcttct                                                                >  2:3586558/1‑101 (MQ=255)
                     tgggcAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCcttctt                                                               >  2:4075373/5‑101 (MQ=255)
                     tgggcAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCcttctt                                                               >  1:4112138/5‑101 (MQ=255)
                     tgggcAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGGTCGGTCCTTCACGTTCTCcttctt                                                               >  2:847714/5‑101 (MQ=255)
                       ggcAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgg                                                             >  1:94124/3‑101 (MQ=255)
                       ggcAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgg                                                             <  1:937322/99‑1 (MQ=255)
                       ggcAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgg                                                             >  1:1771099/3‑101 (MQ=255)
                        gcAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTggg                                                            <  2:2791135/100‑1 (MQ=255)
                         cAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgggt                                                           <  1:3133888/101‑2 (MQ=255)
                           aggatcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgggtg                                                          >  1:3003424/6‑99 (MQ=255)
                           aggatcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgggtg                                                          >  1:4224519/6‑99 (MQ=255)
                           aggatcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgggtg                                                          <  2:201288/96‑3 (MQ=255)
                            ggatcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgggtgc                                                         <  2:802357/97‑4 (MQ=255)
                            ggatcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgggtgc                                                         <  2:3343588/97‑4 (MQ=255)
                            ggatcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgggtgc                                                         >  1:1005245/5‑98 (MQ=255)
                             gatcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgggtgca                                                        <  2:3588435/98‑5 (MQ=255)
                             gatcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgggtgca                                                        >  1:1442464/4‑97 (MQ=255)
                               tcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GTGTGCAGa                                                     <  1:1380662/100‑1 (MQ=255)
                               tcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGa                                                     <  1:3313861/100‑1 (MQ=255)
                                   gcgccGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGaaaa                                                  >  2:3443459/4‑101 (MQ=255)
                                   gcgccGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGaaaa                                                  >  1:1311595/4‑101 (MQ=255)
                                     gccGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAAcc                                                <  1:1061718/100‑1 (MQ=255)
                                       cggcTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGGTCGGTCATTCACGTTCTCCTTCTT.TGGTGCAGAAAACCAt                                              >  1:2418897/4‑101 (MQ=255)
                                        gTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATc                                             <  1:4091862/101‑1 (MQ=255)
                                        gTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATc                                             <  2:3286161/101‑1 (MQ=255)
                                        gTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATc                                             >  1:2768266/1‑101 (MQ=255)
                                         tCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATcc                                            <  2:1263716/101‑1 (MQ=255)
                                         tCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATcc                                            <  1:1126657/101‑1 (MQ=255)
                                            tCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCcgc                                         >  2:1472077/1‑101 (MQ=255)
                                            tCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCcgc                                         >  2:2711817/1‑101 (MQ=255)
                                            tCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCcgc                                         <  1:2161363/101‑1 (MQ=255)
                                             ggTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCcgcg                                        <  1:471163/100‑1 (MQ=255)
                                             cGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCcgcg                                        >  1:306378/1‑101 (MQ=255)
                                             cGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCcgcg                                        <  2:444812/101‑1 (MQ=255)
                                              gTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCgg                                       <  1:1090802/101‑1 (MQ=255)
                                              gTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCgg                                       >  2:924049/1‑101 (MQ=255)
                                                ggCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGAc                                     >  2:2979695/1‑101 (MQ=255)
                                                 gCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGAcg                                    <  2:3421982/101‑1 (MQ=255)
                                                  cATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGAcgc                                   <  1:699940/101‑1 (MQ=255)
                                                   aTTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGAcgcg                                  <  1:3586811/101‑1 (MQ=255)
                                                   aTTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGAcgcg                                  <  2:2137728/101‑1 (MQ=255)
                                                    ttGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACgcgc                                 >  2:1881540/1‑101 (MQ=255)
                                                      tggcaaGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGcgcc                                <  2:1635288/97‑1 (MQ=255)
                                                        gcaaGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGcgccgc                              <  2:3405222/99‑1 (MQ=255)
                                                         caaGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCcgcg                             <  2:1360855/100‑1 (MQ=255)
                                                          aaGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCgg                            >  1:2554837/1‑101 (MQ=255)
                                                            gCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGa                          <  1:3416721/101‑1 (MQ=255)
                                                            gCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGa                          <  2:2143516/101‑1 (MQ=255)
                                                            gCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGa                          <  2:4055500/101‑1 (MQ=255)
                                                             cTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGAt                         >  2:3168959/1‑101 (MQ=255)
                                                                 cgggcggCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGc                     <  1:2275607/101‑1 (MQ=255)
                                                                  gggcggCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCa                    <  2:604339/101‑1 (MQ=255)
                                                                  gggcggCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCa                    <  2:3458734/101‑1 (MQ=255)
                                                                   ggcggcACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAg                   <  1:1472179/101‑1 (MQ=255)
                                                                    gcggcACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAgg                  <  1:3207871/101‑1 (MQ=255)
                                                                     cggcACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGt                 <  2:2410927/101‑1 (MQ=255)
                                                                      ggcACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGtt                <  2:3367280/101‑1 (MQ=255)
                                                                      ggcACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGtt                >  2:2936790/1‑101 (MQ=255)
                                                                       gcACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTg               <  2:271818/101‑1 (MQ=255)
                                                                         gcTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGaa             <  1:2763245/100‑1 (MQ=255)
                                                                         aCTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGaa             >  1:3347197/1‑101 (MQ=255)
                                                                          cTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGGTGaaa            >  2:1522495/1‑101 (MQ=255)
                                                                           tGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGaaaa           <  2:4095923/101‑1 (MQ=255)
                                                                           tGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGaaaa           <  1:1636696/101‑1 (MQ=255)
                                                                           tGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGaaaa           >  1:2546803/1‑101 (MQ=255)
                                                                            gTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAAt          <  1:2864830/101‑1 (MQ=255)
                                                                            gTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAAt          >  1:1697331/1‑101 (MQ=255)
                                                                              cGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAAtgt        >  2:4212648/1‑101 (MQ=255)
                                                                                   aGCGCCAG.TTGACG.CGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGGTGAAAATGTGTTCt   >  2:1356340/1‑101 (MQ=255)
                                                                                   aGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGTTCt   <  1:617311/101‑1 (MQ=255)
                                                                                    gcgcCAG.TTGACG.CGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGTTCtg  <  2:244721/101‑1 (MQ=255)
                                                                                                                                                                                            
AGCCAGCGCGGCAGTGATCGTGGGCCAGGATACGGCGCCCGTCTTCGTGGCATTGGCAAAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAGTTTGACACCGGTTCGGTCATTCACGTTCTCCTTCTTGGGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGTTCTG  >  NC_014034/2420208‑2420395

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG