Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,449,577 +G 1.8% coding (56/1584 nt) oppA2 → oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,449,5771.→G 1.8% 2.1 ‑3.0 ‑0.0166coding (56/1584 nt)oppA2oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (59/104):  total (62/104)
Fisher's exact test strand distribution p-value = 5.05e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.45e-01

Only 100 of 204 total aligned reads displayed.

CGGGTCCTCCCTGCCCTCCCCACAGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGAAACGCTGGATCCGCACAAGAATTCGACCATCCCCGAGGCGAACCTG  >  NC_014034/2449477‑2449677
                                                                                                     |                                                                                                      
cGGGTCCTCCCTGCCCTCCCCACAGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGc                                                                                                         <  1:2462691/101‑1 (MQ=255)
 gggTCCTCCCTGCCCTCCCCACAGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.g                                                                                                       >  1:531272/1‑101 (MQ=255)
 gggTCCTCCCTGCCCTCCCCACAGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.g                                                                                                       <  2:1140828/101‑1 (MQ=255)
 gggTCCTCCCTGCCCTCCCCACAGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.g                                                                                                       >  2:1274772/1‑101 (MQ=255)
   gTCCTCCCTGCCCTCCCCACAGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCCCCGCCGCCTCGCTGATCGCGCTCATGGC.ggg                                                                                                     >  1:3258612/1‑101 (MQ=255)
    tcctccCTGCCCTCCCCACAGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGt                                                                                                    <  1:3693285/101‑1 (MQ=255)
      ctccCTGCCCTCCCCACAGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTgg                                                                                                  <  2:3192284/101‑1 (MQ=255)
      ctccCTGCCCTCCCCACAGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGgg                                                                                                  >  2:2586769/1‑101 (MQ=255)
       tccCTGCCCTCCCCACAGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGgc                                                                                                 <  2:4130828/101‑1 (MQ=255)
        cccTGCCCTCCCCACAGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGgcg                                                                                                <  1:3818178/101‑1 (MQ=255)
          cTGCCCTCCCCACAGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGGGGGC.t                                                                                             >  2:297433/1‑101 (MQ=255)
           tGCCCTCCCCACAGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.tt                                                                                            <  1:1612776/101‑1 (MQ=255)
            gCCCTCCCCACAGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.ttt                                                                                           <  2:2430023/101‑1 (MQ=255)
            gCCCTCCCCACAGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGgggggctt                                                                                            >  1:4160692/1‑95 (MQ=255)
            gCCCTCCCCACAGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCCGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.ttt                                                                                           <  2:3788885/101‑1 (MQ=255)
             cccTCCCCACAGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGGgcggcttg                                                                                           >  1:1673211/1‑96 (MQ=255)
                 ccccACAGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCg                                                                                      <  2:3551293/101‑1 (MQ=255)
                    cacaGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGaaa                                                                                   <  1:4231381/101‑1 (MQ=255)
                     gatctCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAAc                                                                                  <  2:314103/97‑1 (MQ=255)
                     acaGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAAc                                                                                  <  1:4041031/101‑1 (MQ=255)
                     acaGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGGGGGG.TTTTGGCGGAAc                                                                                  >  2:2752026/1‑101 (MQ=255)
                     acaGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGGGGGC.GTTGGCCGaaaa                                                                                  >  1:2269264/1‑100 (MQ=255)
                       aGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGGGC.GTTGGCCGGAAccc                                                                                >  2:1377099/1‑100 (MQ=255)
                       aGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCg                                                                                <  2:1089989/101‑1 (MQ=255)
                       aGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGGGGGC.TTTGGCCCAAAccc                                                                                >  2:1097517/1‑100 (MQ=255)
                       aGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGGGCGC.GTTGGCCGAAAccc                                                                                >  2:166751/1‑100 (MQ=255)
                         tCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGCGGGGGGGGGT.TTTGGCCGGAAcccg                                                                               >  1:141036/1‑99 (MQ=255)
                         tCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTg                                                                              <  2:2502509/101‑1 (MQ=255)
                         tCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGGGGGC.GTTGGGCGAAAACgcg                                                                              >  2:775227/1‑99 (MQ=255)
                          ccACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.CTTCGGCGAGAccccgc                                                                             >  1:2631414/1‑97 (MQ=255)
                            acaGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCtt                                                                           <  1:1377199/101‑1 (MQ=255)
                                agGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GAcc                                                                      <  2:918786/101‑1 (MQ=255)
                                agGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GAcc                                                                      <  2:1630353/101‑1 (MQ=255)
                                  gAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGGGCGC.TTTGGCCGAAACCGGGCTT.TAcccc                                                                    >  1:1296846/1‑99 (MQ=255)
                                     aCCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGGGCGC.TTTTGCCGAAACCGTGCTT.GACCGCGGc                                                                 >  2:3481/1‑101 (MQ=255)
                                     aCCCCCGCATGACCCTTCGCTTCAAGTCCCAGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGGGGGC.TTTGGGCGCAAACGTGCCT.GCCCGCGGc                                                                 >  1:3767380/1‑101 (MQ=255)
                                      cccccGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGca                                                                <  2:1704047/101‑1 (MQ=255)
                                      cccccGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGca                                                                <  2:3262909/101‑1 (MQ=255)
                                      cccccGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGca                                                                <  2:392833/101‑1 (MQ=255)
                                       ccccGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGGGGGC.GTTGGCCGAAACCGGGCTT.GACCGCGGcaa                                                               >  2:3679884/1‑101 (MQ=255)
                                        cccGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGcaac                                                              <  1:680084/101‑1 (MQ=255)
                                        cccGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGcaac                                                              <  1:58960/101‑1 (MQ=255)
                                          cGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGcaacaa                                                            <  1:1924712/101‑1 (MQ=255)
                                           gCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCaacaac                                                           <  1:2146582/101‑1 (MQ=255)
                                            cATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACg                                                          <  1:3395846/101‑1 (MQ=255)
                                             aTGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGGGGGC.TTTGGCCGAAACCCTGCTT.GACCGCGGcaaccaacg                                                         >  1:294076/1‑96 (MQ=255)
                                              taaaccTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGc                                                        <  1:1016118/97‑1 (MQ=255)
                                              tGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGc                                                        <  1:2627261/101‑1 (MQ=255)
                                              tGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGGGCGC.GTTGGCCGAAACCGAGATTCGACGCCGGC.ACAACGGc                                                        >  2:3402214/1‑101 (MQ=255)
                                               gACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCg                                                       <  2:431957/101‑1 (MQ=255)
                                               gACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGGGGGC.TTTGGCCGAAACCGTGCTT.GACCGCGGccaacacagcg                                                       >  1:133516/1‑91 (MQ=255)
                                               atctcTTCGCTTCAAGTCCCTGCTCCCCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCg                                                       <  1:3306119/97‑1 (MQ=255)
                                                    ttCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCg                                                  <  1:3221158/101‑1 (MQ=255)
                                                     tCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCgg                                                 <  2:3815585/101‑1 (MQ=255)
                                                       gCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGaa                                               <  1:4070130/101‑1 (MQ=255)
                                                       gCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGaa                                               <  2:2631222/101‑1 (MQ=255)
                                                       gCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGGGGGC.TTTGGCCGGAACCGGGCTT.TACCGCGGCAAAAACGGCGGccccgga                                               >  2:823831/1‑97 (MQ=255)
                                                        cTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGaaa                                              <  1:2659417/101‑1 (MQ=255)
                                                        cTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGGGCGCGTTGGGCGGAAACCG.GCTT.GACCCCGGCAACAAAGGGGACCCGGaaa                                              >  1:3992315/1‑101 (MQ=255)
                                                         ttCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGAAAc                                             <  1:1781831/101‑1 (MQ=255)
                                                         ttCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGGGCGC.TTTTGCCGAAACCCTGCTT.GACCGCGGGCACAACGGCGACCCCGGAAc                                             >  1:852655/1‑101 (MQ=255)
                                                          tCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGGGGGC.TTTGGGCGAAAACCGGCTT.GACCGCGGGAACAACAGGGCCCCCGGAACg                                            >  2:901665/1‑101 (MQ=255)
                                                           cAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGGGGGC.TTTTGCCGAAACCCTGCTT.GACCGCGGCAACAACGGCGACCCGGGAACGc                                           >  2:1552002/1‑101 (MQ=255)
                                                             aGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGCGGGGGGGCGC.TTTGGCGGAACCCGTGTTT.GACCCCGGCAAAAACGCGGCCCCGGAAAcccg                                          >  1:1428103/1‑98 (MQ=255)
                                                             aGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGAAACGCTg                                         <  2:3767123/101‑1 (MQ=255)
                                                             aGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGGGCGG.CTTGGCCGAAAACGTGCTT.GACCCCGGCAACAACGGCGACCCCGGAAcacgg                                         >  2:2277469/1‑97 (MQ=255)
                                                              gTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGAAACGCTgg                                        <  1:973568/101‑1 (MQ=255)
                                                                cccTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGGGGGC.TTTTGCCGAAAACGTGCTT.GACCGCGGCAACAACGGCGACCCCGAAACGCTgggg                                      >  1:2472516/1‑99 (MQ=255)
                                                                  cTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGAAACGCTGGATcc                                    <  1:1081203/101‑1 (MQ=255)
                                                                  cTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGAAACGCTGGATcc                                    <  2:417777/101‑1 (MQ=255)
                                                                  cTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGGGGGC.TTTGGGCGAAACCCGGCTT.TGCCCCGGCAAAAACCGAGGACCGGCAACGATGGagac                                    >  2:4198702/1‑98 (MQ=255)
                                                                   tGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGAAACGCTGGATCCg                                   <  2:497434/101‑1 (MQ=255)
                                                                   tGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGGGGGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCCACAACGGGGCCCCCGAAAAGCTGGATCCg                                   >  1:3167325/1‑101 (MQ=255)
                                                                    gCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGGGGGC.TTTTGCCGAAACCGTGCTT.TACCCCGGCAACAACGGCGGCCCGGGAAAGCTGGATCCGc                                  >  1:2771304/1‑101 (MQ=255)
                                                                     cTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGGGC.TTTTGCCCAAACCCTGCTT.GACCCCGGCAACAACAGCGACCCGGAAAAGCTGGATCCCca                                 >  2:3963409/1‑101 (MQ=255)
                                                                      tCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGGGGGGG.GTTTGCCGGAACCGTGCTT.GACCGCGGCCACAAAGGCGACCCGCAAACGCCGGGTCCGccc                                >  1:2715046/1‑99 (MQ=255)
                                                                        aCCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGAAACGCTGGATCCGCACaa                              <  1:1157707/101‑1 (MQ=255)
                                                                             cgccTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGAAACGCTGGATCCGCAAAAGAAtt                         <  1:3882617/101‑1 (MQ=255)
                                                                              gccTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGAAACGCTGGATCCGCACAAGAATTc                        <  2:3184274/101‑1 (MQ=255)
                                                                               ccTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGAAACGCTGGATCCGCACAAGAATTCg                       <  1:1685370/101‑1 (MQ=255)
                                                                               ccTCGCTGATCGCGCTCATGGC.GGGGGGGGC.TTTTGCCCCAACCGTGCTT.TACCCCGGCAACAACGGCGGCCCGGGAACGCCGGGTCCGCACAAGAAGTCg                       >  2:626150/1‑101 (MQ=255)
                                                                                cTCGCTGATCGCGCTCATGGC.GGGGGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCCACAACGGCGACCCCGGAAAGCCGGGTCCGCACAAGAATTCGa                      >  2:1156978/1‑101 (MQ=255)
                                                                                cTCGCTGATCGCGCTCATGGC.GGGGGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGAAACGCTGGATCCGCACAAGAATTCGa                      >  1:4075475/1‑101 (MQ=255)
                                                                                 tCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGAAACGCTGGATCCGCACAAGAATTCGaa                     <  2:4021952/101‑2 (MQ=255)
                                                                                 tCGCTGATCGCGCTCATGGC.GGGGGGGGC.TTTTGCCGAAACCCTGCTT.GACCGCGGCAACAAAGGCGACCCGGGAAAGCTGGATCCGCACAAGAATTCGAc                     >  1:363890/1‑101 (MQ=255)
                                                                                  cGCTGATCGCGCTCATGGC.GGGGGGCGC.TTTTGCCGAAACCGTGCTT.TACCGCGGCAACAACGGCGACCCGGAAACGCTGGATCCGCACCAGAATTCGAcc                    >  2:1739229/1‑101 (MQ=255)
                                                                                  cGCTGATCGCGCTCATGGC.GGGGGGCGC.TTTTGCCGAAACCCCGCTT.GACCGCGGCAACAACGGCGACCCCGAAACGCCGGATCCGCACAAGAAATCGAcc                    >  2:1968124/1‑101 (MQ=255)
                                                                                   gCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGAAACGCTGGATCCGCACAAGAATTCGACCa                   <  2:2855591/101‑1 (MQ=255)
                                                                                    cTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGAAACGCTGGATCCGCACAAGAATTCGACCAt                  <  1:3948917/101‑1 (MQ=255)
                                                                                    cTGATCGCGCTCATGGC.GGGGGGGGC.TTTTGCCGGAACCCTGCTT.GACCGCGGCAACAACGGCGACCCCGGAACGCTGGATCCGCACAAGAATTCGACCAt                  >  1:2595359/1‑101 (MQ=255)
                                                                                    cTGATCGCGCTCATGGC.GGGGGGCGC.TTTTGCCGAAACCGTGCTT.TACCGCGGCAACAACGGCGACCCGGAAACGCTGGATCCCCACAAAAAATCGAccct                  >  2:672312/1‑99 (MQ=255)
                                                                                       aTCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGAAACGCTGGATCCGCACAAGAATTCGACCATccc               <  2:2887774/101‑1 (MQ=255)
                                                                                       aTCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGAAACGCTGGATCCGCACAAGAATTCGACCATccc               <  2:2424320/101‑1 (MQ=255)
                                                                                        tCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGAAACGCTGGATCCGCACAAGAATTCGACCATcccc              <  2:203693/101‑1 (MQ=255)
                                                                                         cgcgCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGAAACGCTGGATCCGCACAAGAATTCGACCATCCCCg             <  2:1373383/101‑1 (MQ=255)
                                                                                         cgcgCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGAAACGCTGGATCCGCACAAGAATTCGACCATCCCCg             <  2:4072234/101‑1 (MQ=255)
                                                                                                aTGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGAAACGCTGGATCCGCACAAGAATTCGACCATCCCCGAGGCGaa      <  1:3680138/101‑1 (MQ=255)
                                                                                                 tGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGAAACGCTGGATCCGCACGAGAATTCGACCATCCCCGAGGCGAAc     >  1:3550272/1‑101 (MQ=255)
                                                                                                   gC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGAAACGCTGGATCCGCACAAGAATTCGACCATCCCCGAGGCGAACCt   <  1:3139335/101‑1 (MQ=255)
                                                                                                   gC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGAAACGCTGGATCCGCACAAGAATTCGACCATCCCCGAGGCGAACCt   <  1:2218813/101‑1 (MQ=255)
                                                                                                     |                                                                                                      
CGGGTCCTCCCTGCCCTCCCCACAGTCCACAGAGGAAACCCCCGCATGACCCTTCGCTTCAAGTCCCTGCTCACCGCCGCCTCGCTGATCGCGCTCATGGC.GGGTGGCGC.TTTGGCCGAAACCGTGCTT.GACCGCGGCAACAACGGCGACCCGGAAACGCTGGATCCGCACAAGAATTCGACCATCCCCGAGGCGAACCTG  >  NC_014034/2449477‑2449677

Base quality scores:  ATCG  < 3 ≤  ATCG  < 26 ≤  ATCG  < 32 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG