Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,493,952 +G 97.0% coding (252/252 nt) RCAP_rcc02319 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,493,9521.→G97.0% 4.7 ‑1.2 ‑0.5133coding (252/252 nt)RCAP_rcc02319hypothetical protein
Reads supporting (aligned to +/- strand):  new base (40/89):  ref base (0/4):  total (40/93)
Fisher's exact test strand distribution p-value = 3.15e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.01e-01

Only 100 of 171 total aligned reads displayed.

GAGCCCCGAAGAGCGCCGCGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.A..GGGG.GGG.CGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCG  >  NC_014034/2493853‑2494047
                                                                                                      |                                                                                                  
gAGCCCCGAAGAGCGCCGCGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.A..g                                                                                                  >  2:1658054/1‑101 (MQ=255)
  gCCCCGAAGAGCGCCGCGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGGGG.A.A..ggg                                                                                                >  2:3776462/1‑101 (MQ=255)
      cGAAGAGCGCCGCGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACCGGCCGAGG.A.A..gggg.ggg                                                                                           >  1:4031804/1‑101 (MQ=255)
       taaGAGCGCCGCGCCGTGTTGGCCTAGCCGCCCTCCCGCCCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGA.A.A..GGGG.GGg.c                                                                                         <  2:350654/100‑1 (MQ=255)
       gAAGAGCGCCGCGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGGACGACAGGCCGGGG.A.A..gggg.gggg                                                                                          >  2:2594424/1‑100 (MQ=255)
       gAAGAGCGCCGCGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.A..gggg.gggg                                                                                          <  1:2538861/101‑2 (MQ=255)
       gAAGAGCGCCGCGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAAG.A.A..gggg.gggg                                                                                          >  1:1353757/1‑100 (MQ=255)
        aaGGGCTCCGCGCGGTGATGGCCGATCGGCCCACCCGCGCTCCTCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.A..gggg.ggggc                                                                                         <  1:485270/101‑3 (MQ=255)
        aaGAGCGCCGCGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.A..gggg.ggggg                                                                                         >  2:3038860/1‑99 (MQ=255)
        aaGAGCGCCGCGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGG.A.A..gggg.ggggg                                                                                         >  2:1455340/1‑99 (MQ=255)
        aaGAGCGCCGCGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGAGGGCGCGCGGCACGACAGGCCGCGG.C.A..gggg.ggggc                                                                                         >  2:485213/1‑99 (MQ=255)
        aaGAGCGCCGCGCGGTGATGGCCGAGCGGCCCGCCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.A..gggg.ggggc                                                                                         <  2:3645735/101‑3 (MQ=255)
         agagCGCCGCGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.A..gggg.gggggg                                                                                        >  1:2354696/1‑98 (MQ=255)
         agagCGCCGCGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.A..gggg.ggggcg                                                                                        <  1:3957724/101‑4 (MQ=255)
            gcgcCGCGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTg                                                                                     <  1:1522254/101‑1 (MQ=255)
             cgccgcGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.A..gggg.gggggggcgg                                                                                    >  1:2178992/1‑94 (MQ=255)
               ccgcGCGGTGATGGCCGAGCTGCCCCCCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGccc                                                                                  <  2:448146/101‑1 (MQ=255)
                cgcgcgGTGATGGCCGAGCTGCCCCCCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGcccc                                                                                 <  1:3684789/101‑1 (MQ=255)
                cgcgcgGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGcccc                                                                                 <  2:207632/101‑1 (MQ=255)
                cgcgcgGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGcccc                                                                                 >  2:4113289/1‑101 (MQ=255)
                 gcgcgGTGGTGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGccccc                                                                                <  1:3470076/101‑1 (MQ=255)
                  cgcgGTGAGGGCCGAGCTGCCCCCCCGCGCGCCGCGTCTTGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGcccccc                                                                               <  1:1510523/101‑2 (MQ=255)
                   ggggggATGGCCGGGCTGCCCCCCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGccccccg                                                                              <  2:3595282/96‑3 (MQ=255)
                   gcgGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGGGT.A.AG.GGGG.GGG.GGCTGccccccg                                                                              >  1:2449543/1‑99 (MQ=255)
                    cgGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGGGTCA.G..GGGG.GGG.GGCTGccccccgt                                                                             >  2:3330965/1‑98 (MQ=255)
                       tGATGGCCGAGCTGCCCCCCCGCGCCCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCg                                                                          <  1:2137498/101‑1 (MQ=38)
                        gATGGCCGAGCTGCCCCCCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGc                                                                         <  1:3291161/101‑1 (MQ=38)
                          tGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCaa                                                                       <  1:1783186/101‑1 (MQ=38)
                             ccGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCGGCCCCCCCCCCGCCAGCg                                                                    >  1:3567160/1‑101 (MQ=255)
                                  cTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTc                                                               >  2:2180163/1‑101 (MQ=37)
                                   tGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.GGCTGCCCCCCGTCCGCCAGCGGACCcc                                                              >  1:3009047/1‑101 (MQ=37)
                                      ccACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTccccc                                                           <  2:1224431/101‑1 (MQ=37)
                                       cccccGCGCCCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTcccccc                                                          <  2:3945830/99‑1 (MQ=25)
                                        aCCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGG.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCCCAAGCGGACTCCCCCCg                                                         >  2:2553374/1‑101 (MQ=25)
                                          ccGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCggg                                                       <  1:581234/101‑1 (MQ=25)
                                          ccGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCggg                                                       <  2:2144297/101‑1 (MQ=25)
                                            gcgcgcCGCGTCTGGCCCAGCGCCGGGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGat                                                     <  1:2488481/101‑1 (MQ=25)
                                            gcgcgcCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGat                                                     <  2:1241319/101‑1 (MQ=25)
                                            gcgcgcCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGat                                                     <  1:2285558/101‑1 (MQ=25)
                                            gcgcgcCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGat                                                     <  1:1737812/101‑1 (MQ=25)
                                            gcgcgcCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGat                                                     <  2:2852394/101‑1 (MQ=25)
                                             cgcgcCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGGCCCCCAGCGGACTCCCCCCGGGgta                                                    >  2:157809/1‑101 (MQ=25)
                                              gcgcCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGatat                                                   <  2:2078909/101‑1 (MQ=25)
                                              gcgcCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGatat                                                   <  2:2478853/101‑1 (MQ=25)
                                              gccccgcGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGatat                                                   <  1:1455442/98‑1 (MQ=25)
                                                gccgcGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.ACG..GGGG.GGG.CGCTTCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATAttt                                                 <  1:678656/101‑1 (MQ=25)
                                                  cgcgTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgc                                               <  1:1675971/101‑1 (MQ=25)
                                                   gcgTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgca                                              <  1:2111405/101‑1 (MQ=25)
                                                    cgTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgcag                                             <  1:3663506/101‑1 (MQ=25)
                                                    cgTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgcag                                             <  1:4193476/101‑1 (MQ=25)
                                                     gTCTGGCCAAGCAGCGCGGCGGGCGCGCGGGGCGACAGCCTGAGT.A.A..GGGGTGGG.CGATGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgcagc                                            <  2:2670810/101‑1 (MQ=17)
                                                      tCTTGCCCAGCGCCGCGGCGGGCGCGCGGCCCGACAGGCCGAGT.A.A..GGGG.GGGTCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgcagca                                           <  2:4078883/101‑1 (MQ=21)
                                                      tCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgcagca                                           >  2:87518/1‑101 (MQ=25)
                                                      tCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgcagca                                           <  1:1658169/101‑1 (MQ=25)
                                                        tGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAg                                         <  1:2475969/101‑1 (MQ=21)
                                                        tGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAg                                         <  1:1563538/101‑1 (MQ=21)
                                                        tGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAg                                         <  2:599343/101‑1 (MQ=21)
                                                         ggCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCCAGCGGACTCCCCCCGGGATATTTGCAGCAAGa                                        >  1:1784659/1‑101 (MQ=21)
                                                          gCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGAt                                       <  1:3979039/101‑1 (MQ=21)
                                                           cccAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATg                                      <  2:573109/101‑1 (MQ=21)
                                                           cccAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATg                                      <  2:4193359/101‑1 (MQ=21)
                                                             cAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGGTATTTGCAGCAAGATGaa                                    >  1:936623/1‑101 (MQ=21)
                                                             cAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaa                                    <  1:311703/101‑1 (MQ=21)
                                                               gcgcCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAc                                  <  2:3840071/101‑1 (MQ=21)
                                                               gcgcCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CCCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAc                                  <  1:967855/101‑1 (MQ=21)
                                                                 gccgcGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCg                                <  2:2745107/101‑1 (MQ=21)
                                                                 gccgcGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCg                                >  2:2630897/1‑101 (MQ=21)
                                                                 gccgcGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCg                                <  2:2226720/101‑1 (MQ=21)
                                                                  ccgcGGCGGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCgg                               <  1:93742/101‑1 (MQ=21)
                                                                    gcggcgGGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAt                             <  1:3278494/101‑1 (MQ=21)
                                                                     cggcggGCGCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATg                            <  2:1528402/101‑1 (MQ=21)
                                                                      ggcgggcgCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGc                           <  1:2661231/101‑1 (MQ=21)
                                                                         gggcgCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCCGGCTCCCCCCGGGATATTTGCAGCAAGAGGAAACCGGATGCCtt                        >  2:1852319/1‑101 (MQ=21)
                                                                          ggcgCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                       <  2:2677322/101‑1 (MQ=21)
                                                                          ggcgCGCGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                       <  2:3798128/101‑1 (MQ=21)
                                                                           gcgcgcGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                      >  1:2611616/1‑101 (MQ=21)
                                                                           gcgcgcGGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                      <  2:1834049/101‑1 (MQ=21)
                                                                           gcgcgcGGCACGACAGGCCGAGG.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                      <  2:1972186/101‑1 (MQ=18)
                                                                            cgcgcgGCACGACAGGCCGAGT.A.AGGGGGG.GGG.CGCTGCCCCCCGCCCGCAAGCGGACTCCCCCCGGGAATTTTGCAGCAAAATGAAACCGGAGGCCTTTc                      >  1:1059554/1‑101 (MQ=18)
                                                                            cgcgcgGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                     <  1:3896555/101‑1 (MQ=21)
                                                                            cgcgcgGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                     <  1:337161/101‑1 (MQ=21)
                                                                            cccgcgGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                     <  1:1388558/99‑1 (MQ=18)
                                                                             gcgcgGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGACTTTCAt                    >  2:734027/1‑101 (MQ=18)
                                                                               gcgGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                  >  1:3138380/1‑101 (MQ=21)
                                                                               gcgGCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                  <  2:1549294/101‑1 (MQ=21)
                                                                                cggccggACAGGCCGAGT.A.AG.GGGG.GGG.CCCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                 <  1:2413190/95‑1 (MQ=18)
                                                                                  gCACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGc               >  2:1165122/1‑101 (MQ=21)
                                                                                   cACGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGcc              >  1:1014986/1‑101 (MQ=21)
                                                                                    aCGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGGCTTTCATCTTGccc             >  2:3040367/1‑101 (MQ=18)
                                                                                    aCGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc             <  2:2354520/101‑1 (MQ=21)
                                                                                     cGACAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg            >  1:1888872/1‑101 (MQ=21)
                                                                                        cAGGCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa         <  2:3183986/101‑1 (MQ=21)
                                                                                          ggCCGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata       <  1:3201927/101‑1 (MQ=21)
                                                                                             cGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc    <  1:746828/101‑1 (MQ=21)
                                                                                             cGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc    <  1:3496873/101‑1 (MQ=21)
                                                                                             cGAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc    >  1:1615732/1‑101 (MQ=18)
                                                                                              gAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc   <  1:275202/101‑1 (MQ=18)
                                                                                              gAGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCCTGCCCGAAATATccc   >  2:4030906/1‑101 (MQ=18)
                                                                                               aGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg  <  1:2718827/101‑1 (MQ=18)
                                                                                               aGT.A.AG.GGGG.GGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg  <  1:1137456/101‑1 (MQ=18)
                                                                                                      |                                                                                                  
GAGCCCCGAAGAGCGCCGCGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.A.A..GGGG.GGG.CGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCG  >  NC_014034/2493853‑2494047

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG